ES2069061T3 - Reactivos y procedimiento para la determinacion de la actividad de proteasas de retrovirus. - Google Patents

Reactivos y procedimiento para la determinacion de la actividad de proteasas de retrovirus.

Info

Publication number
ES2069061T3
ES2069061T3 ES90870250T ES90870250T ES2069061T3 ES 2069061 T3 ES2069061 T3 ES 2069061T3 ES 90870250 T ES90870250 T ES 90870250T ES 90870250 T ES90870250 T ES 90870250T ES 2069061 T3 ES2069061 T3 ES 2069061T3
Authority
ES
Spain
Prior art keywords
reagents
activity
determination
procedure
fluorogenic
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
ES90870250T
Other languages
English (en)
Inventor
Garland Ross Marshall
Mihaly Vadkerti Toth
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
University of Washington
Washington University in St Louis WUSTL
Original Assignee
University of Washington
Washington University in St Louis WUSTL
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by University of Washington, Washington University in St Louis WUSTL filed Critical University of Washington
Application granted granted Critical
Publication of ES2069061T3 publication Critical patent/ES2069061T3/es
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/005—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from viruses
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/34—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
    • C12Q1/37—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase involving peptidase or proteinase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2740/00—Reverse transcribing RNA viruses
    • C12N2740/00011—Details
    • C12N2740/10011—Retroviridae
    • C12N2740/16011—Human Immunodeficiency Virus, HIV
    • C12N2740/16211—Human Immunodeficiency Virus, HIV concerning HIV gagpol
    • C12N2740/16222—New viral proteins or individual genes, new structural or functional aspects of known viral proteins or genes

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biophysics (AREA)
  • Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Molecular Biology (AREA)
  • Wood Science & Technology (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Virology (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Analytical Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Peptides Or Proteins (AREA)
  • Enzymes And Modification Thereof (AREA)

Abstract

SE REVELA UN NUEVO SUSTRATO FLUOROGENICO PARA PROTASA RETROVIRAL, P.EJ. PROTASA HIV, CON LA ESTRUCTURA QUIMICA DE X-THR-LLE-NLE-PHE(Y)-GLN-ARG -NH SUB 2 EN LA QUE X ES UN GRUPO FLUOROGENICO E Y ES UN ACEPTOR PARA EL GRUPO FLUOROGENICO; Y SU USO EN UN METODO FLUOROGENICO PARA DETERMINAR LA PROTASA RETROVIRAL.
ES90870250T 1989-12-28 1990-12-27 Reactivos y procedimiento para la determinacion de la actividad de proteasas de retrovirus. Expired - Lifetime ES2069061T3 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US07/458,060 US5011910A (en) 1989-12-28 1989-12-28 Reagent and method for determining activity of retroviral protease

Publications (1)

Publication Number Publication Date
ES2069061T3 true ES2069061T3 (es) 1995-05-01

Family

ID=23819193

Family Applications (1)

Application Number Title Priority Date Filing Date
ES90870250T Expired - Lifetime ES2069061T3 (es) 1989-12-28 1990-12-27 Reactivos y procedimiento para la determinacion de la actividad de proteasas de retrovirus.

Country Status (8)

Country Link
US (1) US5011910A (es)
EP (1) EP0435845B1 (es)
JP (1) JP2909225B2 (es)
AT (1) ATE116334T1 (es)
CA (1) CA2033254C (es)
DE (1) DE69015603T2 (es)
DK (1) DK0435845T3 (es)
ES (1) ES2069061T3 (es)

Families Citing this family (30)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
SE8801702D0 (sv) * 1988-05-05 1988-05-05 Pharmacia Ab Sett vid bestemning av enzymatisk aktivitet
DE3842197A1 (de) * 1988-12-15 1990-06-21 Hoechst Ag Rasch spaltbares substrat fuer die hiv-protease
US5171662A (en) * 1990-09-13 1992-12-15 The Upjohn Company Method of detecting HIV protease activity
DE69333270T2 (de) 1992-03-11 2004-08-05 Narhex Ltd. Aminderivate von oxo- und hydroxy- substituierten kohlenwasserstoffen
US6071895A (en) * 1992-03-11 2000-06-06 Narhex Limited Polar-substituted hydrocarbons
JPH07504654A (ja) * 1992-03-11 1995-05-25 ナルヘックス　リミテッド オキソ及びヒドロキシ置換炭化水素のアミン誘導体
US5888992A (en) * 1992-03-11 1999-03-30 Narhex Limited Polar substituted hydrocarbons
GB9310978D0 (en) * 1993-05-27 1993-07-14 Zeneca Ltd Compounds
US5506115A (en) * 1994-04-29 1996-04-09 G. D. Searle & Co. Reagent and method for determining activity of herpes protease
US5605809A (en) * 1994-10-28 1997-02-25 Oncoimmunin, Inc. Compositions for the detection of proteases in biological samples and methods of use thereof
US5976857A (en) * 1996-01-26 1999-11-02 Boehringer Mannheim Corporation Cross-linked polypeptide fragments of β-galactosidase
EP0817789B1 (en) * 1996-01-26 2002-01-02 Roche Diagnostics Corporation Bis-maleimido cross-linking agents
US5763196A (en) * 1996-01-26 1998-06-09 Boehringer Mannheim Corporation Assays using cross-linked polypeptide fragments of β-galactosidase
EP0906334A1 (en) * 1996-04-24 1999-04-07 Peptide Therapeutics Limited Auto-deconvoluting combinatorial libraries
US6893868B2 (en) * 1997-02-20 2005-05-17 Onco Immunin, Inc. Homo-doubly labeled compositions for the detection of enzyme activity in biological samples
US6037137A (en) 1997-02-20 2000-03-14 Oncoimmunin, Inc. Fluorogenic peptides for the detection of protease activity
US7312302B2 (en) 1997-02-20 2007-12-25 Oncolmmunin, Inc. Compositions for the detection of enzyme activity in biological samples and methods of use thereof
US20060040319A1 (en) * 2000-01-14 2006-02-23 Tom Muir Multiple sensor-containing active modified polypeptides, preparation and uses thereof
GB0024351D0 (en) * 2000-10-04 2000-11-22 Amersham Pharm Biotech Uk Ltd Dye-labelled peptide and method
US7473678B2 (en) * 2004-10-14 2009-01-06 Biomimetic Therapeutics, Inc. Platelet-derived growth factor compositions and methods of use thereof
WO2007061889A2 (en) * 2005-11-17 2007-05-31 Biomimetic Therapeutics, Inc. Maxillofacial bone augmentation using rhpdgf-bb and a biocompatible matrix
EP1991282B1 (en) * 2006-02-09 2013-06-19 BioMimetic Therapeutics, LLC Compositions and methods for treating bone
CA2656278C (en) 2006-06-30 2016-02-09 Biomimetic Therapeutics, Inc. Compositions and methods for treating rotator cuff injuries
US9161967B2 (en) * 2006-06-30 2015-10-20 Biomimetic Therapeutics, Llc Compositions and methods for treating the vertebral column
US8106008B2 (en) 2006-11-03 2012-01-31 Biomimetic Therapeutics, Inc. Compositions and methods for arthrodetic procedures
ES2422259T3 (es) * 2008-02-07 2013-09-10 Biomimetic Therapeutics Inc Composiciones para la osteogénesis por distracción
KR20110067035A (ko) 2008-09-09 2011-06-20 바이오미메틱 세라퓨틱스, 인크. 건 및 인대 손상의 치료를 위한 혈소판-유래 성장 인자 조성물 및 방법
BRPI0923015A2 (pt) * 2008-12-19 2015-12-15 Biomimetic Therapeutics Inc enxertos ósseos com atividade de protease reduzida e métodos de seleção e uso
AU2010221089A1 (en) * 2009-03-05 2011-09-15 Biomimetic Therapeutics, Inc. Platelet-derived growth factor compositions and methods for the treatment of osteochondral defects
CN107080834A (zh) 2010-02-22 2017-08-22 生物模拟治疗有限责任公司 用于治疗腱病的血小板衍生生长因子组合物和方法

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3384555A (en) * 1965-11-30 1968-05-21 Army Usa Direct fluorometric method for measuring dehydrogenase activity
DE3244030A1 (de) * 1982-11-27 1984-05-30 Behringwerke Ag, 3550 Marburg Chromogene verbindungen, verfahren zu ihrer herstellung und ihre verwendung
DE3531778A1 (de) * 1985-09-06 1987-03-12 Behringwerke Ag Verfahren zur bestimmung von proteaseinhibitoren

Also Published As

Publication number Publication date
JPH04210598A (ja) 1992-07-31
CA2033254C (en) 2001-11-20
DK0435845T3 (da) 1995-05-01
DE69015603D1 (de) 1995-02-09
US5011910A (en) 1991-04-30
EP0435845B1 (en) 1994-12-28
CA2033254A1 (en) 1991-06-29
ATE116334T1 (de) 1995-01-15
DE69015603T2 (de) 1995-06-22
EP0435845A1 (en) 1991-07-03
JP2909225B2 (ja) 1999-06-23

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