ES2072235T3 - Metodo y reactivo para determinar variaciones especificas de nucleotidos. - Google Patents

Metodo y reactivo para determinar variaciones especificas de nucleotidos.

Info

Publication number
ES2072235T3
ES2072235T3 ES91903942T ES91903942T ES2072235T3 ES 2072235 T3 ES2072235 T3 ES 2072235T3 ES 91903942 T ES91903942 T ES 91903942T ES 91903942 T ES91903942 T ES 91903942T ES 2072235 T3 ES2072235 T3 ES 2072235T3
Authority
ES
Spain
Prior art keywords
detection step
variable nucleotide
incorporation
primer
reagent
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
ES91903942T
Other languages
English (en)
Other versions
ES2072235T1 (es
Inventor
Hans Soderlund
Ann-Christine Syvanen
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Sangtec Medical AB
Original Assignee
Sangtec Medical AB
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Sangtec Medical AB filed Critical Sangtec Medical AB
Publication of ES2072235T1 publication Critical patent/ES2072235T1/es
Application granted granted Critical
Publication of ES2072235T3 publication Critical patent/ES2072235T3/es
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6883—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6858—Allele-specific amplification
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6881—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for tissue or cell typing, e.g. human leukocyte antigen [HLA] probes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6883—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material
    • C12Q1/6886—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material for cancer
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/70—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving virus or bacteriophage
    • C12Q1/701—Specific hybridization probes
    • C12Q1/702—Specific hybridization probes for retroviruses
    • C12Q1/703—Viruses associated with AIDS
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/156—Polymorphic or mutational markers

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Engineering & Computer Science (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Genetics & Genomics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • Virology (AREA)
  • Pathology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Hospice & Palliative Care (AREA)
  • Oncology (AREA)
  • AIDS & HIV (AREA)
  • Cell Biology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Heterocyclic Carbon Compounds Containing A Hetero Ring Having Oxygen Or Sulfur (AREA)
  • Measuring Pulse, Heart Rate, Blood Pressure Or Blood Flow (AREA)
  • Investigating Or Analyzing Non-Biological Materials By The Use Of Chemical Means (AREA)
  • Compositions Of Macromolecular Compounds (AREA)
  • Saccharide Compounds (AREA)
  • Nitrogen And Oxygen Or Sulfur-Condensed Heterocyclic Ring Systems (AREA)

Abstract

LA DETECCION DE NUCLEOTIDO(S) VARIABLE(S) SE BASA EN UNA EXTENSION IMPRIMADORA E INCORPORACION DE TRIFOSFATOS DE NUCLEOSIDOS DETECTABLES. SI SE SELECCIONAN LOS IMPRIMADORES DEL PASO DE DETECCION DE LA REGION JUSTO ADYACENTE AL NUCLEOTIDO VARIABLE, ESTA VARIACION SE PUEDE DETECTAR TRAS LA INCORPORACION DE UN SOLO TRIFOSFATO DE NUCLEOSIDO. SE AÑADEN TRIFOSFATOS DE NUCLEOSIDOS ETIQUETADOS QUE COINCIDAN CON EL NUCLEOTIDO VARIABLE Y SE MIDE LA INCORPORACION DE UNA ETIQUETA EN EL IMPRIMADOR DEL PASO DE DETECCION. LA SELECCION DEL IMPRIMADOR DEL PASO DE DETECCION ES IMPORTANTE PARA EL METODO DE ESTA INVENCION Y DEPENDE DE LA SECUENCIA DE NUCLEOTIDOS DE INTERES. LOS IMPRIMADORES DEL PASO DE DETECCION SE SELECCIONAN PREFERENTEMENTE DE MODO QUE EXTIENDAN LA REGION INMEDIATAMENTE HACIA EL TERMINAL 3'' DESDE EL NUCLEOTIDO VARIABLE A DETECTAR. ESTE METODO ES UTIL PARA IDENTIFICAR LAS MUTACIONES Y VARIACIONES GENETICAS EN PUNTOS ESPECIFICOS.
ES91903942T 1990-02-16 1991-02-15 Metodo y reactivo para determinar variaciones especificas de nucleotidos. Expired - Lifetime ES2072235T3 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US48200590A 1990-02-16 1990-02-16

Publications (2)

Publication Number Publication Date
ES2072235T1 ES2072235T1 (es) 1995-07-16
ES2072235T3 true ES2072235T3 (es) 1999-07-16

Family

ID=23914258

Family Applications (1)

Application Number Title Priority Date Filing Date
ES91903942T Expired - Lifetime ES2072235T3 (es) 1990-02-16 1991-02-15 Metodo y reactivo para determinar variaciones especificas de nucleotidos.

Country Status (18)

Country Link
EP (1) EP0648280B1 (es)
JP (1) JP2786011B2 (es)
AT (1) ATE180019T1 (es)
AU (1) AU642709B2 (es)
CA (1) CA2071537C (es)
DE (2) DE648280T1 (es)
DK (1) DK0648280T3 (es)
ES (1) ES2072235T3 (es)
FI (1) FI102297B (es)
GR (2) GR950300047T1 (es)
HU (1) HU211058B (es)
IE (1) IE910525A1 (es)
IL (1) IL97222A (es)
NO (1) NO311529B1 (es)
NZ (1) NZ237134A (es)
PT (1) PT96776B (es)
WO (1) WO1991013075A2 (es)
ZA (1) ZA911152B (es)

Families Citing this family (80)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5547839A (en) 1989-06-07 1996-08-20 Affymax Technologies N.V. Sequencing of surface immobilized polymers utilizing microflourescence detection
US6013431A (en) 1990-02-16 2000-01-11 Molecular Tool, Inc. Method for determining specific nucleotide variations by primer extension in the presence of mixture of labeled nucleotides and terminators
US6004744A (en) 1991-03-05 1999-12-21 Molecular Tool, Inc. Method for determining nucleotide identity through extension of immobilized primer
WO1992016180A2 (en) * 1991-03-13 1992-10-01 Siska Diagnostics, Inc. Detection of 3'-azido-3'-deoxythymidine resistance
US7026115B1 (en) 1991-09-24 2006-04-11 Keygene N.V. Selective restriction fragment amplification: fingerprinting
US20100267023A1 (en) 1992-09-24 2010-10-21 Keygene N.V. Selective restriction fragment amplification: fingerprinting
GB9208000D0 (en) * 1992-04-10 1992-05-27 Univ London Quantitative viral assay
US5750335A (en) * 1992-04-24 1998-05-12 Massachusetts Institute Of Technology Screening for genetic variation
ATE198358T1 (de) * 1992-04-27 2001-01-15 Dartmouth College Detektion von gensequenzen in biologischen flüssigkeiten
GB9210176D0 (en) * 1992-05-12 1992-06-24 Cemu Bioteknik Ab Chemical method
US5981176A (en) * 1992-06-17 1999-11-09 City Of Hope Method of detecting and discriminating between nucleic acid sequences
CA2115342C (en) * 1992-06-17 2003-08-26 Robert B. Wallace A method of detecting and discriminating between nucleic acid sequences
DE69232846T2 (de) * 1992-06-17 2003-08-21 City Of Hope, Duarte Verfahren zum nachweis und unterscheiden von nukleinsäuresequenzen
US5710028A (en) * 1992-07-02 1998-01-20 Eyal; Nurit Method of quick screening and identification of specific DNA sequences by single nucleotide primer extension and kits therefor
ATE231881T1 (de) * 1992-07-02 2003-02-15 Savyon Diagnostics Ltd Verfahren zur einfachen nukleotid-primer- verlängerung zum nachweis spezifischer allele und dafür geeigneter kit
US5605798A (en) 1993-01-07 1997-02-25 Sequenom, Inc. DNA diagnostic based on mass spectrometry
US6153379A (en) * 1993-06-22 2000-11-28 Baylor College Of Medicine Parallel primer extension approach to nucleic acid sequence analysis
US7001722B1 (en) 1993-06-22 2006-02-21 Baylor College Of Medicine Parallel primer extension approach to nucleic acid sequence analysis
US6071699A (en) 1996-06-07 2000-06-06 California Institute Of Technology Nucleic acid mediated electron transfer
CA2111503A1 (en) * 1993-12-15 1995-06-16 Mcgill University Apolipoprotein e polymorphism and alzheimer's disease
US6428955B1 (en) 1995-03-17 2002-08-06 Sequenom, Inc. DNA diagnostics based on mass spectrometry
GB9507238D0 (en) 1995-04-07 1995-05-31 Isis Innovation Detecting dna sequence variations
US5830655A (en) 1995-05-22 1998-11-03 Sri International Oligonucleotide sizing using cleavable primers
GB9517914D0 (en) * 1995-09-02 1995-11-01 Tepnel Medical Ltd Identification of bases in nucleic acid sequences
NO954667D0 (no) * 1995-11-17 1995-11-17 Dagfinn Oegreid Fremgangsmåte til deteksjon av Ki-ras mutasjoner
WO1997035033A1 (en) 1996-03-19 1997-09-25 Molecular Tool, Inc. Method for determining the nucleotide sequence of a polynucleotide
EP0933431A1 (en) * 1996-07-11 1999-08-04 Wakunaga Pharmaceutical Co., Ltd. Method for examining nucleic acids and examination kits
EP2264045B1 (en) 1996-08-14 2015-10-21 Life Technologies Corporation Stable compositions for nucleic acid amplification and sequencing
GB9620209D0 (en) 1996-09-27 1996-11-13 Cemu Bioteknik Ab Method of sequencing DNA
US7381525B1 (en) 1997-03-07 2008-06-03 Clinical Micro Sensors, Inc. AC/DC voltage apparatus for detection of nucleic acids
US7045285B1 (en) 1996-11-05 2006-05-16 Clinical Micro Sensors, Inc. Electronic transfer moieties attached to peptide nucleic acids
US6096273A (en) 1996-11-05 2000-08-01 Clinical Micro Sensors Electrodes linked via conductive oligomers to nucleic acids
US6140053A (en) 1996-11-06 2000-10-31 Sequenom, Inc. DNA sequencing by mass spectrometry via exonuclease degradation
EP1164203B1 (en) 1996-11-06 2007-10-10 Sequenom, Inc. DNA Diagnostics based on mass spectrometry
US6133436A (en) * 1996-11-06 2000-10-17 Sequenom, Inc. Beads bound to a solid support and to nucleic acids
GB9626815D0 (en) 1996-12-23 1997-02-12 Cemu Bioteknik Ab Method of sequencing DNA
US5945284A (en) * 1997-05-27 1999-08-31 The Perkin-Elmer Corporation Length determination of nucleic acid repeat sequences by discontinuous primer extension
US6309829B1 (en) 1997-05-27 2001-10-30 Pe Corporation (Ny) Length determination of nucleic acid repeat sequences by discontinuous primer extension
US6566101B1 (en) 1997-06-16 2003-05-20 Anthony P. Shuber Primer extension methods for detecting nucleic acids
US5888778A (en) * 1997-06-16 1999-03-30 Exact Laboratories, Inc. High-throughput screening method for identification of genetic mutations or disease-causing microorganisms using segmented primers
GB9715034D0 (en) * 1997-07-18 1997-09-24 Zeneca Ltd Assay
US7105353B2 (en) 1997-07-18 2006-09-12 Serono Genetics Institute S.A. Methods of identifying individuals for inclusion in drug studies
EP0892068A1 (en) * 1997-07-18 1999-01-20 Genset Sa Method for generating a high density linkage disequilibrium-based map of the human genome
US7745142B2 (en) 1997-09-15 2010-06-29 Molecular Devices Corporation Molecular modification assays
US7070921B2 (en) 2000-04-28 2006-07-04 Molecular Devices Corporation Molecular modification assays
US7632651B2 (en) 1997-09-15 2009-12-15 Mds Analytical Technologies (Us) Inc. Molecular modification assays
US6982431B2 (en) 1998-08-31 2006-01-03 Molecular Devices Corporation Sample analysis systems
US6537751B1 (en) 1998-04-21 2003-03-25 Genset S.A. Biallelic markers for use in constructing a high density disequilibrium map of the human genome
CA2245039A1 (en) * 1998-08-13 2000-02-13 Vito Scalia Primer-specific and mispair extension assay for identifying gene variation
JP2002523111A (ja) * 1998-08-28 2002-07-30 サンテック モレキュラー ダイアグノスティックス エービー 特定医薬を代謝することに対する患者の能力を測定する方法
DE19855469C2 (de) * 1998-12-01 2001-01-11 Michael Esrich Verfahren zur Bestimmung des Apolipoprotein-E-Genotyps in einer menschlichen Probe
US6503718B2 (en) 1999-01-10 2003-01-07 Exact Sciences Corporation Methods for detecting mutations using primer extension for detecting disease
US6280947B1 (en) 1999-08-11 2001-08-28 Exact Sciences Corporation Methods for detecting nucleotide insertion or deletion using primer extension
US6573047B1 (en) 1999-04-13 2003-06-03 Dna Sciences, Inc. Detection of nucleotide sequence variation through fluorescence resonance energy transfer label generation
WO2000063437A2 (en) 1999-04-20 2000-10-26 Illumina, Inc. Detection of nucleic acid reactions on bead arrays
US20060275782A1 (en) 1999-04-20 2006-12-07 Illumina, Inc. Detection of nucleic acid reactions on bead arrays
US7056661B2 (en) 1999-05-19 2006-06-06 Cornell Research Foundation, Inc. Method for sequencing nucleic acid molecules
JP2001245698A (ja) * 1999-11-22 2001-09-11 Xiao Bing Wang 核酸検出法
IL149676A0 (en) 1999-12-02 2002-11-10 Dna Sciences Inc Methods and kits for determining nucleotide variations
US6518024B2 (en) 1999-12-13 2003-02-11 Motorola, Inc. Electrochemical detection of single base extension
US6762018B1 (en) 1999-12-23 2004-07-13 Tetragen Sa Analysis of nucleotide polymorphisms at a site
ATE412774T1 (de) 2000-02-16 2008-11-15 Illumina Inc Parallele genotypisierung mehrerer patientenproben
US6355433B1 (en) 2000-06-02 2002-03-12 Dna Sciences, Inc. Determination of nucleotide sequence variations through limited primer extension
US6958214B2 (en) 2000-07-10 2005-10-25 Sequenom, Inc. Polymorphic kinase anchor proteins and nucleic acids encoding the same
SG115374A1 (en) * 2000-07-17 2005-10-28 Xiao Bing Wang Detection of sequence variation of nucleic acid by shifted termination analysis
CH699253B1 (de) * 2000-09-18 2010-02-15 Eidgenoessische Forschungsanst Verfahren zur Charakterisierung und/oder Identifikation von Genomen.
AU2002227156A1 (en) 2000-12-01 2002-06-11 Visigen Biotechnologies, Inc. Enzymatic nucleic acid synthesis: compositions and methods for altering monomer incorporation fidelity
US6428964B1 (en) 2001-03-15 2002-08-06 Exact Sciences Corporation Method for alteration detection
US6803201B2 (en) 2002-01-24 2004-10-12 Stratagene Compositions and methods for polynucleotide sequence determination
WO2003093296A2 (en) 2002-05-03 2003-11-13 Sequenom, Inc. Kinase anchor protein muteins, peptides thereof, and related methods
JP4143756B2 (ja) 2002-06-21 2008-09-03 財団法人名古屋産業科学研究所 心筋梗塞のリスク診断方法
DE10245145B4 (de) * 2002-09-27 2004-12-02 IPK-Institut für Pflanzengenetik und Kulturpflanzenforschung Verfahren zum Nachweis von SNPs auf polydimensionalen Microarrays
AU2003280603A1 (en) 2002-10-29 2004-05-25 Kabushiki Kaisha Dnaform Method of amplifying nucleic acid
US8206902B2 (en) 2003-12-25 2012-06-26 Riken Method of amplifying nucleic acid and method of detecting mutated nucleic acid using the same
US7170050B2 (en) 2004-09-17 2007-01-30 Pacific Biosciences Of California, Inc. Apparatus and methods for optical analysis of molecules
CA2579150C (en) 2004-09-17 2014-11-25 Pacific Biosciences Of California, Inc. Apparatus and method for analysis of molecules
US9109256B2 (en) 2004-10-27 2015-08-18 Esoterix Genetic Laboratories, Llc Method for monitoring disease progression or recurrence
US9777314B2 (en) 2005-04-21 2017-10-03 Esoterix Genetic Laboratories, Llc Analysis of heterogeneous nucleic acid samples
EP4230747A3 (en) 2008-03-28 2023-11-15 Pacific Biosciences Of California, Inc. Compositions and methods for nucleic acid sequencing
WO2012049279A1 (en) 2010-10-14 2012-04-19 Universitaet Des Saarlandes MEANS AND METHODS APPLYING SINGLE NUCLEOTIDE PRIMER EXTENSION WITH ION PAIR-, REVERSED-PHASE HPLC (SIRPH) FOR THE DIAGNOSIS OF SNPs

Family Cites Families (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB8311018D0 (en) * 1983-04-22 1983-05-25 Amersham Int Plc Detecting mutations in dna
US4851331A (en) * 1986-05-16 1989-07-25 Allied Corporation Method and kit for polynucleotide assay including primer-dependant DNA polymerase
AU622104B2 (en) * 1987-03-11 1992-04-02 Sangtec Molecular Diagnostics Ab Method of assaying of nucleic acids, a reagent combination and kit therefore
FI80476C (fi) * 1987-10-09 1990-06-11 Orion Yhtymae Oy Foerbaettrat hybridisationsfoerfarande, vid foerfarandet anvaent redskap och reagensfoerpackning.
IE61148B1 (en) * 1988-03-10 1994-10-05 Ici Plc Method of detecting nucleotide sequences
DE68916671T2 (de) * 1988-03-18 1995-03-02 Baylor College Medicine Mutationsnachweis durch kompetitiven Oligonukleotid-Priming.
AU3539089A (en) * 1988-04-08 1989-11-03 Salk Institute For Biological Studies, The Ligase-based amplification method
AU3694689A (en) * 1988-04-28 1989-11-24 Mark H. Skolnick Amplified sequence polymorphisms (asps)
AU629845B2 (en) * 1988-08-30 1992-10-15 Abbott Laboratories Detection and amplification of target nucleic acid sequences
CA2002076A1 (en) * 1988-11-21 1990-05-21 Brent A. Burdick Diagnostic kit and method using a solid phase capture means for detecting nucleic acids
DK175170B1 (da) * 1988-11-29 2004-06-21 Sangtec Molecular Diagnostics Fremgangsmåde og reagenskombination til bestemmmelse af nukleotidsekvenser
IE66572B1 (en) * 1989-02-13 1996-01-24 Geneco Pty Ltd Detection of a nucleic acid sequence or a change therein
AU5640090A (en) * 1989-03-21 1990-11-05 Collaborative Research Inc. A dna diagnostic test using an exonuclease activity

Also Published As

Publication number Publication date
ATE180019T1 (de) 1999-05-15
GR950300047T1 (en) 1995-07-31
AU642709B2 (en) 1993-10-28
EP0648280A1 (en) 1995-04-19
NO923116L (no) 1992-08-10
NO311529B1 (no) 2001-12-03
WO1991013075A3 (en) 1991-10-17
WO1991013075A2 (en) 1991-09-05
NZ237134A (en) 1992-06-25
DE648280T1 (de) 1995-11-30
IL97222A0 (en) 1992-05-25
CA2071537A1 (en) 1991-08-17
FI102297B1 (fi) 1998-11-13
JPH05504477A (ja) 1993-07-15
FI923653A0 (fi) 1992-08-14
EP0648280B1 (en) 1999-05-12
GR3030790T3 (en) 1999-11-30
NO923116D0 (no) 1992-08-10
HU211058B (en) 1995-10-30
IE910525A1 (en) 1991-08-28
ES2072235T1 (es) 1995-07-16
ZA911152B (en) 1991-11-27
DK0648280T3 (da) 1999-11-01
CA2071537C (en) 2003-02-11
PT96776B (pt) 1998-05-29
FI102297B (fi) 1998-11-13
DE69131233T2 (de) 1999-11-04
IL97222A (en) 1995-08-31
FI923653L (fi) 1992-08-14
HU910516D0 (en) 1991-09-30
PT96776A (pt) 1991-10-31
DE69131233D1 (de) 1999-06-17
AU7235191A (en) 1991-09-18
HUT61330A (en) 1992-12-28
JP2786011B2 (ja) 1998-08-13

Similar Documents

Publication Publication Date Title
ES2072235T3 (es) Metodo y reactivo para determinar variaciones especificas de nucleotidos.
Huang et al. A protein from human placental nuclei binds preferentially to 5-methylcytosine-rich DNA
Gray et al. The intracellular phosphorylation of (−)-2′-deoxy-3′-thiacytidine (3TC) and the incorporation of 3TC 5′-monophosphate into DNA by HIV-1 reverse transcriptase and human DNA polymerase γ
DE69702649D1 (de) Fluoreszenz-nachweistest für homogene pcr-hybridisierungssysteme
ATE432284T1 (de) Einen starren linker enthaltende nukleotide
ATE247716T1 (de) Ligase/polymerase-vermittelte analyse genetischer elemente von einzelnukleotid-polymorphismen und ihre verwendung in der genetischen analyse
DK0547056T3 (da) Fremgangsmåde til bestemmelse af polymeraseaktivitet
BR9911395A (pt) Método para detecção de mutações selecionadas por droga no gene protease de hiv
Coupar et al. The Mechanism by which Heparin Stimulates Transcription in Isolated Rat Liver Nuclei: Polyribonucleotide Elongation Rates and the Number of Transcribing RNA Polymerase Molecules Present
DK0443823T3 (da) Mængdebestemmelse af en DNA-polymerase og et testsæt dertil
SE8804344D0 (sv) Saett och substrat foer att aktivitetsbestaemma polymeraser
WO1996011937A1 (en) Porphyrin labeling of polynucleotides
Yamaguchi et al. A photolabile 2′, 3′-dideoxyuridylate analog bearing an aryl (trifluoromethyl) diazirine moiety: photoaffinity labeling of HIV-1 reverse transcriptase
JP4188228B2 (ja) Ｄｎａ重合の測定方法およびその方法の応用
Hoggard et al. Influence of prior exposure to zidovudine on stavudine phosphorylation in vivo and ex vivo
AU2002303065A1 (en) A method for measuring DNA polymerization and applications of the method
Delahunty et al. Studies on primer binding of HIV-1 reverse transcriptase using a fluorescent probe
Coene et al. A microanalytical procedure for determination of the base composition of DNA
Ghe et al. Photometric and electrochemical detection of purine and pyrimidine compounds in reversed-phase and ion-exchange high-pressure liquid chromatography
Baillon et al. Continuous microspectrophotometric measurement of DNA polymerase activity: application to the Klenow fragment of Escherichia coli DNA polymerase I and human immunodeficiency virus type 1 reverse transcriptase.
Shipilov et al. A conserved asparagine makes an essential contact to an RNA adenosine or cytidine
Czarnecki Interactions of 8-azido-2'-deoxyadenosine 5'-triphosphate, a photoaffinity probe, with a DNA-dependent DNA polymerase

Legal Events

Date Code Title Description
FG2A Definitive protection

Ref document number: 648280

Country of ref document: ES