ES2073451T3 - Vectores de clonacion y de expresion en una cepa de actinomiceto, procedimiento de transformacion de dicha cepa, cepa de actinomiceto obtenida y preparacion de proteinas. - Google Patents

Vectores de clonacion y de expresion en una cepa de actinomiceto, procedimiento de transformacion de dicha cepa, cepa de actinomiceto obtenida y preparacion de proteinas.

Info

Publication number
ES2073451T3
ES2073451T3 ES89401347T ES89401347T ES2073451T3 ES 2073451 T3 ES2073451 T3 ES 2073451T3 ES 89401347 T ES89401347 T ES 89401347T ES 89401347 T ES89401347 T ES 89401347T ES 2073451 T3 ES2073451 T3 ES 2073451T3
Authority
ES
Spain
Prior art keywords
strain
expression
cloning
actinomicet
actinomicate
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
ES89401347T
Other languages
English (en)
Inventor
Tamara Smokvina
Frederic Boccard
Michel Guerineau
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Centre National de la Recherche Scientifique CNRS
Original Assignee
Centre National de la Recherche Scientifique CNRS
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Centre National de la Recherche Scientifique CNRS filed Critical Centre National de la Recherche Scientifique CNRS
Application granted granted Critical
Publication of ES2073451T3 publication Critical patent/ES2073451T3/es
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2408—Glucanases acting on alpha -1,4-glucosidic bonds
    • C12N9/2411—Amylases
    • C12N9/2414—Alpha-amylase (3.2.1.1.)
    • C12N9/2417—Alpha-amylase (3.2.1.1.) from microbiological source
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • C12N15/76—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for Actinomyces; for Streptomyces
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90—Stable introduction of foreign DNA into chromosome

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Medicinal Chemistry (AREA)
  • Mycology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Saccharide Compounds (AREA)

Abstract

VECTOR DE CLONAJE Y DE EXPRESION DE UNA SECUENCIA DE ADN CODIFICADA PARA UNA PROTEINA DETERMINADA EN UNA CEPA DE ACTIOMICETE. SE CARACTERIZA POR TENER: UNA SECUENCIA DE ENLACE ATT; - UNA SECUENCIA DE ADN CODIFICADA PARA UNA SECUENCIA INT FUNCIONAL EN DICHA CEPA; Y - UNA SECUENCIA DE ADN CODIFICADA PARA DICHA PROTEINA ESPECIFICA Y PARA LOS ELEMENTOS QUE ASEGURAN LA EXPRESION DE DICHA SECUENCIA EN DICHA CEPA.
ES89401347T 1988-05-18 1989-05-16 Vectores de clonacion y de expresion en una cepa de actinomiceto, procedimiento de transformacion de dicha cepa, cepa de actinomiceto obtenida y preparacion de proteinas. Expired - Lifetime ES2073451T3 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
FR8806638A FR2631634B1 (fr) 1988-05-18 1988-05-18 Vecteurs de clonage et d'expression dans une souche d'actinomycete, procede de transformation de cette souche, souche d'actynomycete obtenue et preparation de proteines

Publications (1)

Publication Number Publication Date
ES2073451T3 true ES2073451T3 (es) 1995-08-16

Family

ID=9366396

Family Applications (1)

Application Number Title Priority Date Filing Date
ES89401347T Expired - Lifetime ES2073451T3 (es) 1988-05-18 1989-05-16 Vectores de clonacion y de expresion en una cepa de actinomiceto, procedimiento de transformacion de dicha cepa, cepa de actinomiceto obtenida y preparacion de proteinas.

Country Status (8)

Country Link
US (1) US5688689A (es)
EP (1) EP0350341B1 (es)
JP (1) JP2913406B2 (es)
AT (1) ATE122399T1 (es)
CA (1) CA1340746C (es)
DE (1) DE68922533T2 (es)
ES (1) ES2073451T3 (es)
FR (1) FR2631634B1 (es)

Families Citing this family (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2713658B1 (fr) * 1993-12-08 1996-04-12 Rhone Poulenc Rorer Sa Séquences d'acides nucléiques régulatrices et utilisations.
US6825012B2 (en) 1995-02-23 2004-11-30 Gencell S.A. DNA molecules, preparation and use in gene therapy
FR2731014B1 (fr) * 1995-02-23 1997-03-28 Rhone Poulenc Rorer Sa Molecules d'adn, preparation et utilisation en therapie genique
US7255989B1 (en) 1999-11-29 2007-08-14 Aventis Pharma S.A. Method for obtaining nucleic acids from an environment sample, resulting nucleic acids and use in synthesis of novel compounds
US6861513B2 (en) 2000-01-12 2005-03-01 Schering Corporation Everninomicin biosynthetic genes
US7220567B2 (en) 2000-05-17 2007-05-22 Schering Corporation Isolation of Micromonospora carbonacea var africana pMLP1 integrase and use of integrating function for site-specific integration into Micromonospora halophitica and Micromonospora carbonacea chromosome
FR2921663A1 (fr) 2007-10-02 2009-04-03 Bluestar Silicones France Soc Polyorganosiloxanes a fonction piperidine depourvus de toxicite par contact cutane et utilisation de ces derniers dans des compositions cosmetiques
DE102011118018B4 (de) 2011-10-25 2017-10-26 Plasmidfactory Gmbh & Co. Kg Minicircles mit Transpositionskassetten und ihre Verwendung zur Transformation von Zellen
DE102013220859B4 (de) 2013-10-15 2016-09-08 Plasmidfactory Gmbh & Co. Kg Minicircles mit viralen Expressionskassetten und ihre Verwendung zur Transformation von Zellen zur Erzeugung rekombinanter Viren oder viraler Genvektoren

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4743546A (en) * 1985-02-13 1988-05-10 Biotechnica International, Inc. Controlled gene excision
ATE131535T1 (de) * 1986-02-28 1995-12-15 Smithkline Beecham Corp Das streptomyces-gal-operon
DE3614310A1 (de) * 1986-04-28 1987-10-29 Hoechst Ag Verfahren zur isolierung mutierter gene sowie der entsprechenden wildtyp-gene

Also Published As

Publication number Publication date
FR2631634B1 (fr) 1991-06-14
FR2631634A1 (fr) 1989-11-24
ATE122399T1 (de) 1995-05-15
US5688689A (en) 1997-11-18
DE68922533D1 (de) 1995-06-14
EP0350341A1 (fr) 1990-01-10
EP0350341B1 (fr) 1995-05-10
JPH0269185A (ja) 1990-03-08
CA1340746C (fr) 1999-09-14
DE68922533T2 (de) 1995-10-19
JP2913406B2 (ja) 1999-06-28

Similar Documents

Publication Publication Date Title
ES2056817T3 (es) Transformacion de trichoderma.
ES2149149T1 (es) Proteinas de fijacion de interleuquina-18, su preparacion y utilizacion.
AR243234A1 (es) Un gen quimerico modificado que codifica una proteina insecticida de bacillus thuringiensis,un vector que lo contiene, y un metodo para mejorar la expresion de un gen heterologo.
ES2181674T3 (es) Dominios de union en las proteinas notch y delta.
ES8401133A1 (es) Metodo para preparar albumina de suero humano o una proteina de fusion de la misma.
ES2153037T3 (es) Lacasas purificadas de scytalidium y acidos nucleicos que las codifican.
DK0765394T3 (da) Oprensede Myceliopthora-laccaser og nukleinsyrer der koder for disse
BR9713294A (pt) "anticorpos contra proteìna relacionada a hormÈnio de paratiróide humano"
DK0700445T3 (da) Stressproteiner og anvendelser deraf
ES2196031T3 (es) Derivados peptidicos terapeutico.
CO4810339A1 (es) Nuevas estructuras artificiales de proteinas de fusion y metodo de preparacion
ES8600780A1 (es) Materiales y metodos de exploracion y amplificacion en celulas hospedantes eucarioticas utilizando dihidrofolato-reduc- tasa resistente a metotrexato.
ES2009906A6 (es) Procedimiento de preparacion de transformantes bacterianos.
DK0479948T3 (da) Sammensætninger og fremgangsmåder til syntese af natriuretisk protein-receptor B og anvendelsesfremgangsmåder
DE59708952D1 (de) Fusionspolypeptid zur beeinflussung von wechselwirkungen zwischen proteinen
NZ205241A (en) Synthetic human (pre)prorelaxin; synthetic signal, a, b and c peptide chains thereof; preparation of human preprorelaxin, prorelaxin and relaxin by recombinant dna or other techniques
ES2129487T3 (es) Peptidos sinteticos, anticuerpos dirigidos contra ellos y su utilizacion.
DK0532587T3 (da) Fremgangsmåder til trans-destabilisering af specifikke proteiner in vivo
DK3688A (da) Pankreatisk sekretorisk trypsininhibitor og varianter deraf fremstillet af en rekombinant vaert, fremgangsmaade, ekspressionsvektor og rekombinant vaert samt farmaceutisk anvendelse deraf
ES2191031T3 (es) Proteinas intracelulares de enlace (pil) y sus utilizaciones.
DE69434384D1 (de) Metalkomplexbildner
ES2073451T3 (es) Vectores de clonacion y de expresion en una cepa de actinomiceto, procedimiento de transformacion de dicha cepa, cepa de actinomiceto obtenida y preparacion de proteinas.
ES548617A0 (es) Procedimiento para disociar peptidos y proteinas en el enla-ce metionilo
NO971059L (no) Modifiserte human-C3-proteiner
DE3881023D1 (de) Proteine, expressionsvektoren, die fuer solche proteine kodierende dna enthalten und zellen, die solche expressionsvektoren enthalten.

Legal Events

Date Code Title Description
FG2A Definitive protection

Ref document number: 350341

Country of ref document: ES