ES2081679T3 - Un procedimiento para clonar dna metilado en un huesped bacteriano incapaz de restriccion por el huesped. - Google Patents
Un procedimiento para clonar dna metilado en un huesped bacteriano incapaz de restriccion por el huesped.Info
- Publication number
- ES2081679T3 ES2081679T3 ES93200367T ES93200367T ES2081679T3 ES 2081679 T3 ES2081679 T3 ES 2081679T3 ES 93200367 T ES93200367 T ES 93200367T ES 93200367 T ES93200367 T ES 93200367T ES 2081679 T3 ES2081679 T3 ES 2081679T3
- Authority
- ES
- Spain
- Prior art keywords
- guest
- incapable
- restriction
- bacterial
- procedure
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
- 238000010367 cloning Methods 0.000 title abstract 3
- 230000001580 bacterial effect Effects 0.000 title abstract 2
- 238000000034 method Methods 0.000 title abstract 2
- 108091028043 Nucleic acid sequence Proteins 0.000 abstract 3
- 241000644323 Escherichia coli C Species 0.000 abstract 1
- 239000013600 plasmid vector Substances 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/70—Vectors or expression systems specially adapted for E. coli
- C12N15/73—Expression systems using phage (lambda) regulatory sequences
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6827—Hybridisation assays for detection of mutation or polymorphism
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Genetics & Genomics (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Microbiology (AREA)
- General Health & Medical Sciences (AREA)
- Molecular Biology (AREA)
- Biophysics (AREA)
- Physics & Mathematics (AREA)
- Biochemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Plant Pathology (AREA)
- Analytical Chemistry (AREA)
- Immunology (AREA)
- Crystallography & Structural Chemistry (AREA)
- Virology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Enzymes And Modification Thereof (AREA)
Abstract
ESTA INVENCION SE REFIERE A UN PROCESO PARA LA CLONACION DE UNA SECUENCIA DE ADN EN DONDE UN VECTOR QUE CONTIENE DICHA SECUENCIA DE ADN SE MULTIPLICA EN UN HUESPED BACTERIANO ADECUADO. COMO HUESPED BACTERIANO SE UTILIZA LA CEPA BACTERIANA QUE ES INCAPAZ DE RESTRINGIR HUESPEDES, TAL COMO UNA CEPA C DE ESCHERICHIA COLI. PREFERENTEMENTE, COMO VECTOR SE UTILIZA UN VECTOR BACTERIOFAGO QUE SEA UN VEHICULO DE CLONACION ADECUADO PARA EL HUESPED EN CUESTION, REALIZANDOSE EL PASO DE EMPAQUETAMIENTO IN VIVO EN REVESTIMIENTOS DE FAGOS MEDIANTE UN EXTRACTO DE EMPAQUETAMIENTO DE FAGOS OBTENIDO A PARTIR DE UNA CEPA BACTERIANA QUE ES INCAPAZ DE RESTRINGIR HUESPEDES. LA INVENCION SE PUEDE UTILIZAR EN UN PROCESO PARA DETECTAR LAS MUTACIONES PRODUCIDAS EN UNO O MAS GENES MARCADORES INTRODUCIDOS EN UN GENOMA MAMIFERO MEDIANTE LA UTILIZACION DE UN VECTOR, CUYO PROCESO CONSISTE EN SEPARAR EL ADN DE LAS CELULAS DEL MAMIFERO TRANSGENICO O DE LAS CELULAS MAMIFERAS, RECUPERAR EL VECTOR DE DICHO ADN, MULTIPLICAR EL VECTOR EN UN HUESPED BACTERIANO ADECUADO QUE RESULTA DEFICIENTE RESPECTO A AL MENOS UNO DE LOS GENES MARCADORES Y ANALIZAR LA EXPRESION DEL GEN MARCADOR. PREFERENTEMENTE, EL ADN AISLADO DE LAS CELULAS DEL MAMIFERO TRANSGENICO O DE LAS CELULAS MAMIFERAS SE PREPURIFICA A BASE DE FRAGMENTAR EL ADN MEDIANTE UNA ENZIMA DE RESTRICCION QUE NO CONTENGA UN LADO CORTANTE DENTRO DEL VECTOR, SE SEPARAN LOS FRAGMENTOS RESULTANTES EN BASE A UN CRITERIO ADECUADO, TAL COMO POR TAMAÑOS Y SE RECOGEN LOS FRAGMENTOS QUE CONTIENEN EL VECTOR, TRAS LO CUAL SE RECUPERA EL VECTOR DE DICHO ADN PREPURIFICADO.
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| NL8801826A NL8801826A (nl) | 1988-07-19 | 1988-07-19 | Werkwijze voor het detecteren van mutaties in markergenen. |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| ES2081679T3 true ES2081679T3 (es) | 1996-03-16 |
Family
ID=19852648
Family Applications (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| ES93200367T Expired - Lifetime ES2081679T3 (es) | 1988-07-19 | 1989-07-18 | Un procedimiento para clonar dna metilado en un huesped bacteriano incapaz de restriccion por el huesped. |
| ES89201895T Expired - Lifetime ES2061943T3 (es) | 1988-07-19 | 1989-07-18 | Un procedimiento para el rescate de adn y para detectar mutaciones en genes marcadores. |
Family Applications After (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| ES89201895T Expired - Lifetime ES2061943T3 (es) | 1988-07-19 | 1989-07-18 | Un procedimiento para el rescate de adn y para detectar mutaciones en genes marcadores. |
Country Status (8)
| Country | Link |
|---|---|
| US (1) | US5470706A (es) |
| EP (3) | EP0548056B2 (es) |
| JP (1) | JPH0829093B2 (es) |
| AT (2) | ATE132529T1 (es) |
| DE (2) | DE68912228T2 (es) |
| ES (2) | ES2081679T3 (es) |
| GR (1) | GR3018927T3 (es) |
| NL (1) | NL8801826A (es) |
Families Citing this family (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| NL9100567A (nl) * | 1991-04-02 | 1992-11-02 | Ingeny Bv | Werkwijze voor het detecteren van mutaties, transgeen zoogdier, transgene zoogdiercel, en werkwijze voor het onderzoeken van stoffen of omstandigheden op mutagene eigenschappen. |
| EP0671955A4 (en) * | 1992-02-14 | 1997-04-09 | Stratagene Inc | MUTAGENESE TEST USING NON-HUMAN BEINGS CONTAINING TEST DNA SEQUENCES. |
| AU4220093A (en) * | 1992-04-27 | 1993-11-29 | Indiana University Foundation | Live animal mutagenesis systems for testing mutagenic agents in vivo |
| WO1994017208A1 (en) * | 1993-01-21 | 1994-08-04 | President And Fellows Of Harvard College | Methods and diagnostic kits utilizing mammalian stress promoters to determine toxicity of a compound |
| AU4216099A (en) | 1998-05-31 | 1999-12-20 | University Of Georgia Research Foundation, Inc., The | Bacteriophage-based transgenic fish for mutation detection |
| WO2000024246A2 (en) | 1998-10-26 | 2000-05-04 | The University Of Georgia Research Foundation, Inc. | Transgenic fish carrying plasmid for mutation detection and methods |
| GB9924973D0 (en) * | 1999-10-21 | 1999-12-22 | Cobra Therapeutics Ltd | Preparation of cellular components that are substantially free of chromosomal DNA |
| CZ20033143A3 (en) * | 2001-05-21 | 2004-04-14 | Aclara Biosciences, Inc. | Methods and compositions for analyzing proteins |
Family Cites Families (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4753874A (en) * | 1985-07-08 | 1988-06-28 | Board Of Trustees Of The Leland Stanford Junior University | Rapid mutation testing system for human cells |
| EP0289121B1 (en) * | 1987-05-01 | 1995-12-27 | Stratagene | Mutagenesis testing using transgenic non-human animals carrying test DNA sequences |
-
1988
- 1988-07-19 NL NL8801826A patent/NL8801826A/nl not_active Application Discontinuation
-
1989
- 1989-07-18 JP JP1187193A patent/JPH0829093B2/ja not_active Expired - Lifetime
- 1989-07-18 EP EP93200367A patent/EP0548056B2/en not_active Expired - Lifetime
- 1989-07-18 ES ES93200367T patent/ES2081679T3/es not_active Expired - Lifetime
- 1989-07-18 AT AT93200367T patent/ATE132529T1/de not_active IP Right Cessation
- 1989-07-18 AT AT89201895T patent/ATE100151T1/de not_active IP Right Cessation
- 1989-07-18 EP EP89201895A patent/EP0353812B1/en not_active Expired - Lifetime
- 1989-07-18 ES ES89201895T patent/ES2061943T3/es not_active Expired - Lifetime
- 1989-07-18 EP EP95201753A patent/EP0683238A3/en not_active Withdrawn
- 1989-07-18 DE DE68912228T patent/DE68912228T2/de not_active Expired - Lifetime
- 1989-07-18 DE DE68925369T patent/DE68925369T3/de not_active Expired - Lifetime
-
1993
- 1993-01-29 US US08/013,198 patent/US5470706A/en not_active Expired - Lifetime
-
1996
- 1996-02-07 GR GR960400333T patent/GR3018927T3/el unknown
Also Published As
| Publication number | Publication date |
|---|---|
| DE68925369D1 (de) | 1996-02-15 |
| ATE132529T1 (de) | 1996-01-15 |
| JPH0829093B2 (ja) | 1996-03-27 |
| US5470706A (en) | 1995-11-28 |
| EP0353812A2 (en) | 1990-02-07 |
| DE68912228D1 (de) | 1994-02-24 |
| DE68912228T2 (de) | 1994-08-04 |
| GR3018927T3 (en) | 1996-05-31 |
| EP0548056B2 (en) | 2001-04-04 |
| EP0353812B1 (en) | 1994-01-12 |
| ES2061943T3 (es) | 1994-12-16 |
| EP0548056A1 (en) | 1993-06-23 |
| ATE100151T1 (de) | 1994-01-15 |
| NL8801826A (nl) | 1990-02-16 |
| EP0683238A3 (en) | 1995-12-20 |
| EP0353812A3 (en) | 1990-03-07 |
| EP0683238A2 (en) | 1995-11-22 |
| DE68925369T3 (de) | 2001-08-09 |
| EP0548056B1 (en) | 1996-01-03 |
| JPH02142471A (ja) | 1990-05-31 |
| DE68925369T2 (de) | 1996-05-15 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| FG2A | Definitive protection |
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