ES2084857T3 - Procedimiento para la conversion de una proteina fusionada de analogos activos de la motilina humana y purificacion de estos analogos. - Google Patents
Procedimiento para la conversion de una proteina fusionada de analogos activos de la motilina humana y purificacion de estos analogos.Info
- Publication number
- ES2084857T3 ES2084857T3 ES92104331T ES92104331T ES2084857T3 ES 2084857 T3 ES2084857 T3 ES 2084857T3 ES 92104331 T ES92104331 T ES 92104331T ES 92104331 T ES92104331 T ES 92104331T ES 2084857 T3 ES2084857 T3 ES 2084857T3
- Authority
- ES
- Spain
- Prior art keywords
- fused protein
- analogs
- purification
- human
- motiline
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
- 108090000623 proteins and genes Proteins 0.000 title abstract 4
- 102000004169 proteins and genes Human genes 0.000 title abstract 4
- 238000006243 chemical reaction Methods 0.000 title abstract 2
- 238000000034 method Methods 0.000 title abstract 2
- 238000000746 purification Methods 0.000 title abstract 2
- LVRVABPNVHYXRT-BQWXUCBYSA-N 52906-92-0 Chemical class C([C@H](N)C(=O)N[C@H](C(=O)N1CCC[C@H]1C(=O)N[C@@H]([C@@H](C)CC)C(=O)N[C@@H](CC=1C=CC=CC=1)C(=O)N[C@@H]([C@@H](C)O)C(=O)N[C@@H](CC=1C=CC(O)=CC=1)C(=O)NCC(=O)N[C@@H](CCC(O)=O)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCC(N)=O)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](CCSC)C(=O)N[C@@H](CCC(N)=O)C(=O)N[C@@H](CCC(O)=O)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CCC(O)=O)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](CC(N)=O)C(=O)N[C@@H](CCCCN)C(=O)NCC(=O)N[C@@H](CCC(N)=O)C(O)=O)C(C)C)C1=CC=CC=C1 LVRVABPNVHYXRT-BQWXUCBYSA-N 0.000 abstract 4
- BDAGIHXWWSANSR-UHFFFAOYSA-N methanoic acid Natural products OC=O BDAGIHXWWSANSR-UHFFFAOYSA-N 0.000 abstract 2
- 244000005700 microbiome Species 0.000 abstract 2
- OSWFIVFLDKOXQC-UHFFFAOYSA-N 4-(3-methoxyphenyl)aniline Chemical compound COC1=CC=CC(C=2C=CC(N)=CC=2)=C1 OSWFIVFLDKOXQC-UHFFFAOYSA-N 0.000 abstract 1
- FFEARJCKVFRZRR-BYPYZUCNSA-N L-methionine Chemical compound CSCC[C@H](N)C(O)=O FFEARJCKVFRZRR-BYPYZUCNSA-N 0.000 abstract 1
- 239000007864 aqueous solution Substances 0.000 abstract 1
- 238000004587 chromatography analysis Methods 0.000 abstract 1
- ATDGTVJJHBUTRL-UHFFFAOYSA-N cyanogen bromide Chemical compound BrC#N ATDGTVJJHBUTRL-UHFFFAOYSA-N 0.000 abstract 1
- 238000011033 desalting Methods 0.000 abstract 1
- 235000019253 formic acid Nutrition 0.000 abstract 1
- 230000002209 hydrophobic effect Effects 0.000 abstract 1
- 238000001696 ion exchange chromatographie Methods 0.000 abstract 1
- 229930182817 methionine Natural products 0.000 abstract 1
- 238000006386 neutralization reaction Methods 0.000 abstract 1
- 238000004366 reverse phase liquid chromatography Methods 0.000 abstract 1
- 239000000243 solution Substances 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/575—Hormones
- C07K14/63—Motilins
Landscapes
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Biophysics (AREA)
- Medicinal Chemistry (AREA)
- Zoology (AREA)
- Biochemistry (AREA)
- Toxicology (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Gastroenterology & Hepatology (AREA)
- Molecular Biology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Endocrinology (AREA)
- Peptides Or Proteins (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
Abstract
PROCESO PARA LA CONVERSION DE PROTEINA FUNDIDA EN ANALOGOS DE MOTILINA HUMANA, TIPO ACTIVO, Y PURIFICAR LOS ANALOGOS. SE DESCRIBE UN PROCESO PARA CONVERTIR UNA PROTEINA FUNDIDA QUE CONTIENE ANALOGOS DE MOTILINA HUMANA DOBLEMENTE REPETIDA UNIDOS A UN RESIDUO DE METIONINA ENTRE ANALOGOS DE MOTILINA VECINA, QUE SE EXPRESA EN UN MICROORGANISMO RESPECTO A ANALOGOS DE MOTILINA BIOLOGICAMENTE ACTIVA MEDIANTE SEPARACION QUIMICA DE LA PROTEINA FUNDIDA Y LA PURIFICACION DE LOS ANALOGOS DE MOTILINA BIOLOGICAMENTE ACTIVA. LA PROTEINA FUNDIDA EXPRESADA EN EL MICROORGANISMO TRANSFORMADO SE SEPARA TRATANDO LA MISMA CON BROMURO DE CIANOGENO EN UNA SOLUCION ACUOSA DE ACIDO FORMICO. TRAS LA NEUTRALIZACION DE LA SOLUCION DE LA REACCION RESULTANTE, SE LLEVA A CABO LA PURIFICACION EN COMBINACION CON OPERACIONES DE DESTILACION Y OPERACIONES DE CROMATOGRAFIA DE UN HIDROFOBICO, CROMATOGRAFIAS DE INTERCAMBIO DE IONES Y FASE INVERSA.
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP3063208A JPH04299990A (ja) | 1991-03-27 | 1991-03-27 | モチリンアナログの精製方法 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| ES2084857T3 true ES2084857T3 (es) | 1996-05-16 |
Family
ID=13222553
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| ES92104331T Expired - Lifetime ES2084857T3 (es) | 1991-03-27 | 1992-03-12 | Procedimiento para la conversion de una proteina fusionada de analogos activos de la motilina humana y purificacion de estos analogos. |
Country Status (6)
| Country | Link |
|---|---|
| EP (1) | EP0505846B1 (es) |
| JP (1) | JPH04299990A (es) |
| AT (1) | ATE132166T1 (es) |
| DE (1) | DE69207037T2 (es) |
| DK (1) | DK0505846T3 (es) |
| ES (1) | ES2084857T3 (es) |
Families Citing this family (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH05112464A (ja) * | 1991-04-09 | 1993-05-07 | Sanwa Kagaku Kenkyusho Co Ltd | 安定化された ［Ｌｅｕ１３−ｍｏｔｉｌｉｎ−Ｈｓｅ 医薬組成物 |
| KR20010005701A (ko) * | 1997-03-24 | 2001-01-15 | 리스 데브라 케이. | 모틸린 상동체 |
| WO2000017336A1 (en) * | 1998-09-19 | 2000-03-30 | Sang Jun Lee | Dna cassette encoding a multimer of a biologically active peptide and a cleavable linker attached thereto and process for preparing the biologically active peptide |
| CN100335499C (zh) * | 2004-10-22 | 2007-09-05 | 吴建中 | C端氨基酸内酯修饰型胸腺素α1及其应用 |
Family Cites Families (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2753274B2 (ja) * | 1988-08-24 | 1998-05-18 | 株式会社三和化学研究所 | モチリン様ポリペプチドの製法並びにそのための組換えｄｎａ及び発現用プラスミド |
| EP0378078A1 (en) * | 1989-01-06 | 1990-07-18 | Sanwa Kagaku Kenkyusho Co., Ltd. | Motilin-like polypeptide and use thereof |
-
1991
- 1991-03-27 JP JP3063208A patent/JPH04299990A/ja active Pending
-
1992
- 1992-03-12 DE DE69207037T patent/DE69207037T2/de not_active Expired - Fee Related
- 1992-03-12 ES ES92104331T patent/ES2084857T3/es not_active Expired - Lifetime
- 1992-03-12 EP EP92104331A patent/EP0505846B1/en not_active Expired - Lifetime
- 1992-03-12 AT AT92104331T patent/ATE132166T1/de not_active IP Right Cessation
- 1992-03-12 DK DK92104331.1T patent/DK0505846T3/da active
Also Published As
| Publication number | Publication date |
|---|---|
| DE69207037T2 (de) | 1996-09-05 |
| JPH04299990A (ja) | 1992-10-23 |
| DK0505846T3 (da) | 1996-05-20 |
| DE69207037D1 (de) | 1996-02-08 |
| EP0505846A1 (en) | 1992-09-30 |
| EP0505846B1 (en) | 1995-12-27 |
| ATE132166T1 (de) | 1996-01-15 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| FG2A | Definitive protection |
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