ES2088495T3 - Procedimiento mejorado de purificacion de proteinas recombinantes y compuestos utiles en dicho procedimiento. - Google Patents

Procedimiento mejorado de purificacion de proteinas recombinantes y compuestos utiles en dicho procedimiento.

Info

Publication number
ES2088495T3
ES2088495T3 ES91907916T ES91907916T ES2088495T3 ES 2088495 T3 ES2088495 T3 ES 2088495T3 ES 91907916 T ES91907916 T ES 91907916T ES 91907916 T ES91907916 T ES 91907916T ES 2088495 T3 ES2088495 T3 ES 2088495T3
Authority
ES
Spain
Prior art keywords
procedure
disclosed
fusion proteins
purification
recombinant proteins
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
ES91907916T
Other languages
English (en)
Inventor
Satish K Sharma
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Pharmacia and Upjohn Co
Original Assignee
Upjohn Co
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Upjohn Co filed Critical Upjohn Co
Application granted granted Critical
Publication of ES2088495T3 publication Critical patent/ES2088495T3/es
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K7/00—Peptides having 5 to 20 amino acids in a fully defined sequence; Derivatives thereof
    • C07K7/04—Linear peptides containing only normal peptide links
    • C07K7/06—Linear peptides containing only normal peptide links having 5 to 11 amino acids
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/005—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from viruses
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K7/00—Peptides having 5 to 20 amino acids in a fully defined sequence; Derivatives thereof
    • C07K7/04—Linear peptides containing only normal peptide links
    • C07K7/08—Linear peptides containing only normal peptide links having 12 to 20 amino acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/62—DNA sequences coding for fusion proteins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/20—Fusion polypeptide containing a tag with affinity for a non-protein ligand
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2740/00—Reverse transcribing RNA viruses
    • C12N2740/00011—Details
    • C12N2740/10011—Retroviridae
    • C12N2740/16011—Human Immunodeficiency Virus, HIV
    • C12N2740/16311—Human Immunodeficiency Virus, HIV concerning HIV regulatory proteins
    • C12N2740/16322—New viral proteins or individual genes, new structural or functional aspects of known viral proteins or genes

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Biophysics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Virology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)

Abstract

SE DESCRIBEN PROTEINAS DE FUSION RECOMBINANTES QUE COMPRENDEN UNA PARTE DE POLIPEPTIDO BIOLOGICAMENTE ACTIVO Y UN PEPTIDO DE QUELACION DE METAL. LAS PROTEINAS DE FUSION CONTIENEN PEPTIDOS DE QUELACION DE METAL QUE TIENEN, AL MENOS, TRES Y, PREFERIBLEMENTE, SEIS RESIDUOS DE HISTIDINA ALTERNANTES. TAMBIEN SE DESCRIBEN PROTEINAS DE FUSION QUE CONTIENEN PEPTIDOS DE QUELACION DE METAL QUE SON SUBSTRATOS PARA LA DIPEPTIDILPEPTIDASA I. ADICIONALMENTE, SE DESCRIBE UN METODO DE OBTENCION DE LOS POLIPEPTIDOS DESEADOS PRODUCIENDO PROTEINAS DE FUSION RECOMBINANTES Y PURIFICANDOLAS CON IONES DE METAL INMOVILIZADOS. ASIMISMO SE DESCRIBE UN EQUIPO PARA LA PURIFICACION DE LOS POLIPEPTIDOS DESEADOS.
ES91907916T 1990-04-09 1991-03-11 Procedimiento mejorado de purificacion de proteinas recombinantes y compuestos utiles en dicho procedimiento. Expired - Lifetime ES2088495T3 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US50660590A 1990-04-09 1990-04-09

Publications (1)

Publication Number Publication Date
ES2088495T3 true ES2088495T3 (es) 1996-08-16

Family

ID=24015282

Family Applications (1)

Application Number Title Priority Date Filing Date
ES91907916T Expired - Lifetime ES2088495T3 (es) 1990-04-09 1991-03-11 Procedimiento mejorado de purificacion de proteinas recombinantes y compuestos utiles en dicho procedimiento.

Country Status (9)

Country Link
EP (1) EP0527778B1 (es)
JP (1) JP3346563B2 (es)
AT (1) ATE139572T1 (es)
AU (1) AU7685791A (es)
DE (1) DE69120409T2 (es)
DK (1) DK0527778T3 (es)
ES (1) ES2088495T3 (es)
GR (1) GR3021095T3 (es)
WO (1) WO1991015589A1 (es)

Families Citing this family (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
HUT69963A (en) * 1990-12-13 1995-09-28 Upjohn Co Non-naturally-occuring fusion proteins and process for preparing them
AU2445495A (en) * 1994-05-09 1995-11-29 Unizyme Laboratories A/S An enzymatic process for producing a desired protein from an amino terminal extended protein
ES2108652B1 (es) * 1996-04-19 1998-07-16 Antibioticos Sa Un procedimiento para modificar la enzima 7/3-(4-carboxibutanamido) cefalosporinacilasa y purificarla en un solo paso cromatografico.
CN1953992A (zh) 2004-06-23 2007-04-25 Usv有限公司 源自胎盘和脑垂体同种型的嵌合体人生长激素和获得所述嵌合体的方法
FR2892725B1 (fr) * 2005-10-31 2011-03-04 Flamel Tech Sa Acides polyglutamiques fonctionnalises par des derives de l'histidine et des groupements hydrophobes et leurs applications notamment therapeutiques
ES2832541T3 (es) * 2007-08-06 2021-06-10 Max Planck Gesellschaft Uso de un quelante inmovilizado para la purificación de polipéptidos recombinantes mediante cromatografía de iones metálicos inmovilizados
CN105273059B (zh) * 2015-11-27 2018-07-20 福州大学 一种章鱼钙螯合蛋白肽及其制备方法
JP7597346B2 (ja) * 2020-02-19 2024-12-10 国立大学法人山口大学 タグペプチド及びポリペプチドの精製方法

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4569794A (en) * 1984-12-05 1986-02-11 Eli Lilly And Company Process for purifying proteins and compounds useful in such process
CA1340522C (en) * 1987-03-10 1999-05-04 Heinz Dobeli Fusion proteins containing neighbouring histidines for improved purification
FI896261A7 (fi) * 1987-06-24 1989-12-22 Novo Nordisk As Menetelmä proteiinin tai polypeptidin valmistamiseksi, polypeptidiä ko odaava DNA-sekvenssi, DNA-sekvenssin sisältämä mikro-organismi sekä po lypeptidi ja sen käyttö farmaseuttisena valmisteena

Also Published As

Publication number Publication date
JP3346563B2 (ja) 2002-11-18
DK0527778T3 (da) 1996-10-21
AU7685791A (en) 1991-10-30
EP0527778B1 (en) 1996-06-19
DE69120409D1 (de) 1996-07-25
DE69120409T2 (de) 1996-11-28
GR3021095T3 (en) 1996-12-31
JPH05505940A (ja) 1993-09-02
ATE139572T1 (de) 1996-07-15
WO1991015589A1 (en) 1991-10-17
EP0527778A1 (en) 1993-02-24

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