ES2091267T3 - Substratos enzimaticos de acridinona cromogena. - Google Patents

Substratos enzimaticos de acridinona cromogena.

Info

Publication number
ES2091267T3
ES2091267T3 ES91113550T ES91113550T ES2091267T3 ES 2091267 T3 ES2091267 T3 ES 2091267T3 ES 91113550 T ES91113550 T ES 91113550T ES 91113550 T ES91113550 T ES 91113550T ES 2091267 T3 ES2091267 T3 ES 2091267T3
Authority
ES
Spain
Prior art keywords
enzyme
hydroxy
acridinone
enzyme substrate
acridin
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
ES91113550T
Other languages
English (en)
Inventor
Paul F Corey
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Bayer AG
Bayer Corp
Original Assignee
Bayer AG
Bayer Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Bayer AG, Bayer Corp filed Critical Bayer AG
Application granted granted Critical
Publication of ES2091267T3 publication Critical patent/ES2091267T3/es
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/34—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
    • C12Q1/40—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase involving amylase
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07D—HETEROCYCLIC COMPOUNDS
    • C07D219/00—Heterocyclic compounds containing acridine or hydrogenated acridine ring systems
    • C07D219/04—Heterocyclic compounds containing acridine or hydrogenated acridine ring systems with hetero atoms or with carbon atoms having three bonds to hetero atoms with at the most one bond to halogen, e.g. ester or nitrile radicals, directly attached to carbon atoms of the ring system
    • C07D219/06—Oxygen atoms
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07D—HETEROCYCLIC COMPOUNDS
    • C07D221/00—Heterocyclic compounds containing six-membered rings having one nitrogen atom as the only ring hetero atom, not provided for by groups C07D211/00 - C07D219/00
    • C07D221/02—Heterocyclic compounds containing six-membered rings having one nitrogen atom as the only ring hetero atom, not provided for by groups C07D211/00 - C07D219/00 condensed with carbocyclic rings or ring systems
    • C07D221/20—Spiro-condensed ring systems
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07F—ACYCLIC, CARBOCYCLIC OR HETEROCYCLIC COMPOUNDS CONTAINING ELEMENTS OTHER THAN CARBON, HYDROGEN, HALOGEN, OXYGEN, NITROGEN, SULFUR, SELENIUM OR TELLURIUM
    • C07F9/00—Compounds containing elements of Groups 5 or 15 of the Periodic Table
    • C07F9/02—Phosphorus compounds
    • C07F9/547—Heterocyclic compounds, e.g. containing phosphorus as a ring hetero atom
    • C07F9/553—Heterocyclic compounds, e.g. containing phosphorus as a ring hetero atom having one nitrogen atom as the only ring hetero atom
    • C07F9/576—Six-membered rings
    • C07F9/64—Acridine or hydrogenated acridine ring systems
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H17/00—Compounds containing heterocyclic radicals directly attached to hetero atoms of saccharide radicals
    • C07H17/02—Heterocyclic radicals containing only nitrogen as ring hetero atoms
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/34—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/54—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving glucose or galactose
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2334/00—O-linked chromogens for determinations of hydrolase enzymes, e.g. glycosidases, phosphatases, esterases
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/90—Enzymes; Proenzymes
    • G01N2333/914—Hydrolases (3)
    • G01N2333/924—Hydrolases (3) acting on glycosyl compounds (3.2)
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00—Chemistry: molecular biology and microbiology
    • Y10S435/805—Test papers

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Microbiology (AREA)
  • General Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Biophysics (AREA)
  • Emergency Medicine (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
  • Saccharide Compounds (AREA)
  • Other In-Based Heterocyclic Compounds (AREA)
  • Polysaccharides And Polysaccharide Derivatives (AREA)
  • Peptides Or Proteins (AREA)
  • Heterocyclic Carbon Compounds Containing A Hetero Ring Having Oxygen Or Sulfur (AREA)
  • Nitrogen And Oxygen Or Sulfur-Condensed Heterocyclic Ring Systems (AREA)

Abstract

COMPUESTOS DE SUSTRATOS DE ENZIMAS DE ACRIDINONA CROMOGENICA QUE CONTIENEN CROMOGENES DE 7-HIDROXI-9H-ACRIDINA-2-ONA DERIVADOS EN LA POSICION 7 -HIDROXI CON UN GRUPO QUE PUEDE DIVIDIRSE ENZIMATICAMENTE Y DISUSTITUIDO EN LA POSICION 9 CON GRUPOS ALQUILO O ARILO, QUE PUEDEN SER IGUALES O DIFERENTES, PREFERIBLEMENTE ALQUILO INFERIOR O FENILO, RESPECTIVAMENTE, O JUNTOS FORMAN UN RESIDUO DE CICLOHEXA-2, 5-DIEN-4-ONA O UN RESIDUO DE 4 -HIDROXICICLOHEXILO, E INTERMEDIOS DE 7-HIDROXI-1, 3-DIHALO-9, 9-DIMETIL -ACRIDIN-2-ONA UTILES PARA PREPARAR LOS NUEVOS COMPUESTOS DE SUSTRATO DE ENZIMA DE ACRIDINONA CROMOGENICA Y METODOS PARA ELLO. EL GRUPO QUE PUEDE DIVIDIRSE ENZIMATICAMENTE ES UN RADICAL DE UN COMPUESTO Y-OH QUE COMPRENDE UNA MITAD DE ENZIMA ESPECIFICA QUE PUEDE DIVIDIRSE MEDIANTE UNA ENZIMA ESPECIFICA EN LA QUE SE LIBERA UNA FORMA DESPROTONADA DEL CROMOGEN CON UNA ABSORBENCIA MAXIMA EN UNA SOLUCION BASICA QUE ES SUSTANCIALMENTE MAYOR QUE LA ABSORBENCIA MAXIMA DEL COMPUESTO DE SUSTRATO DE ENZIMADE ACRIDINONA CROMOGENICA PARA FACILITAR UN CAMBIO DISTINTO EN LA ABSORBENCIA QUE PUEDE SER MEDIDO CON PRECISION Y CORRELACIONADO CON LA CANTIDAD DE ENZIMA PRESENTE EN UNA MUESTRA DE ANALISIS LIQUIDO.
ES91113550T 1986-12-09 1987-11-27 Substratos enzimaticos de acridinona cromogena. Expired - Lifetime ES2091267T3 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US93985586A 1986-12-09 1986-12-09

Publications (1)

Publication Number Publication Date
ES2091267T3 true ES2091267T3 (es) 1996-11-01

Family

ID=25473855

Family Applications (1)

Application Number Title Priority Date Filing Date
ES91113550T Expired - Lifetime ES2091267T3 (es) 1986-12-09 1987-11-27 Substratos enzimaticos de acridinona cromogena.

Country Status (11)

Country Link
EP (1) EP0459536B1 (es)
JP (1) JPH0662569B2 (es)
AT (1) ATE142202T1 (es)
AU (1) AU585472B2 (es)
DE (2) DE3779066D1 (es)
DK (1) DK643887A (es)
ES (1) ES2091267T3 (es)
IE (1) IE60183B1 (es)
IL (1) IL84666A (es)
NO (1) NO175308C (es)
ZA (1) ZA879223B (es)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7186696B2 (en) * 2002-03-07 2007-03-06 Toyama University Reagent and method for measuring amylase
US7183069B2 (en) 2002-03-07 2007-02-27 Toyama University Reagent, method and apparatus for measuring amylase activity
FR2850378B1 (fr) * 2003-01-29 2005-03-04 Bio Merieux Substrats enzymatiques, milieux de culture les contenant, utilisation pour detecter une activite aminopeptidase et/ou differencier des bacteries a gram+ par rapport a des bacteries a gram-
CA2581174A1 (en) * 2004-09-16 2006-03-30 Applera Corporation Fluorescent dye compounds, conjugates and uses thereof
US20120171684A1 (en) 2010-12-29 2012-07-05 Life Technologies Corporation Ddao compounds as fluorescent reference standards

Also Published As

Publication number Publication date
EP0459536B1 (en) 1996-09-04
ZA879223B (en) 1988-08-31
NO875008D0 (no) 1987-12-01
JPH01131192A (ja) 1989-05-24
IE60183B1 (en) 1994-06-15
DE3779066D1 (en) 1992-06-17
NO175308B (no) 1994-06-20
DE3751896D1 (de) 1996-10-10
IE873331L (en) 1988-06-09
IL84666A (en) 1992-02-16
DK643887A (da) 1988-06-10
ATE142202T1 (de) 1996-09-15
NO875008L (no) 1988-06-10
AU585472B2 (en) 1989-06-15
EP0459536A1 (en) 1991-12-04
DE3751896T2 (de) 1997-01-16
DK643887D0 (da) 1987-12-08
AU8206087A (en) 1988-06-09
IL84666A0 (en) 1988-05-31
JPH0662569B2 (ja) 1994-08-17
NO175308C (no) 1994-09-28

Similar Documents

Publication Publication Date Title
ES2039225T3 (es) Sustratos enzimatizados a base de acridinonas cromogenas.
AU621510B2 (en) Composition, device and method of assaying for a peroxidatively active substance
AU623038B2 (en) Improved composition, device and method of assaying for peroxidatively active substances
DK0649535T3 (da) Fremgangsmåde til at forbedre målepræcisionen i optisk biosensor assays med detektering af rumligt dæmpede bølger
JPS56134999A (en) Method and reagent for measuring biological activity of heparin in serum
AU585472B2 (en) Chromogenic acridinone enzyme substrates
CA1265424C (en) FLUOROGENIC INDICATORS BASED ON DIHYDROQUINOLONE AND DIHYDROCOUMARIN FOR HYDROGEN PEROXIDE
DE3473475D1 (en) Process for determining substrate or enzymatic activity
DE3663684D1 (de) Determination of prokallikrein
ZA906998B (en) Process and reagent for the specific determination of pancreatic alpha-amylase
DE69215808D1 (de) Bestimmungsverfahren für Serum-Cholinesterase-Aktivität
SE8402305D0 (sv) Sett att bestemma katepsin b i nervaro av andra proteolytiska enzymer, och dertill anvendbara foreningar
JPS5794300A (en) Novel measuring method
JPS57181699A (en) Determination of drug sensitivity
JPS5736997A (ja) Anjiotenshinhenkankosonokatsuseisokuteiho
ES469000A3 (es) Un metodo fluorometrico para la determinacion de la activi- dad de transferasas y proteasas en muestras de fluidos bio- logicos
SU1415692A3 (ru) Способ получения красителя для определения активности фермента
SU1027615A1 (ru) Способ определени активности протеаз

Legal Events

Date Code Title Description
FG2A Definitive protection

Ref document number: 459536

Country of ref document: ES