ES2091777T3 - Procedimiento para preparar un ensayo inmunologico de transferencia western. - Google Patents

Procedimiento para preparar un ensayo inmunologico de transferencia western.

Info

Publication number
ES2091777T3
ES2091777T3 ES90108710T ES90108710T ES2091777T3 ES 2091777 T3 ES2091777 T3 ES 2091777T3 ES 90108710 T ES90108710 T ES 90108710T ES 90108710 T ES90108710 T ES 90108710T ES 2091777 T3 ES2091777 T3 ES 2091777T3
Authority
ES
Spain
Prior art keywords
preparing
procedure
immunological test
western transfer
hiv
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
ES90108710T
Other languages
English (en)
Inventor
Emerson W Chan
Werner Schulze
William Gerard Robey
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Abbott Laboratories
Original Assignee
Abbott Laboratories
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Abbott Laboratories filed Critical Abbott Laboratories
Application granted granted Critical
Publication of ES2091777T3 publication Critical patent/ES2091777T3/es
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54306—Solid-phase reaction mechanisms
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/558—Immunoassay; Biospecific binding assay; Materials therefor using diffusion or migration of antigen or antibody
    • G01N33/561—Immunoelectrophoresis
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
    • G01N33/56983—Viruses
    • G01N33/56988—HIV or HTLV
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00—Chemistry: molecular biology and microbiology
    • Y10S435/974—Aids related test
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S930/00—Peptide or protein sequence
    • Y10S930/01—Peptide or protein sequence
    • Y10S930/22—Viral peptide or viral protein
    • Y10S930/221—Retrovirus related, or human immunodeficiency virus related, or simian immunodeficiency virus related

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Immunology (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Urology & Nephrology (AREA)
  • Hematology (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Biotechnology (AREA)
  • Food Science & Technology (AREA)
  • Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Cell Biology (AREA)
  • Virology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • AIDS & HIV (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Electrochemistry (AREA)
  • Peptides Or Proteins (AREA)
  • Transition And Organic Metals Composition Catalysts For Addition Polymerization (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
  • Steroid Compounds (AREA)

Abstract

LA PRESENTE INVENCION COMPRENDE UN METODO PERFECCIONADO DE INMUNOENSAYO, QUE IMPLICA LA TRANSFERENCIA SIMULTANEA DE ANTIGENOS MULTIPLES SOBRE UN SOPORTE SOLIDO UNICO. LOS ANTIGENOS QUE PUEDEN UTILIZARSE MEDIANTE ESTE METODO PUEDEN OBTENERSE POR MEDIOS NATURALES O MEDIANTE RECOMBINACIONES, Y SE PUEDEN PURIFICAR SOBRE UNA VARIEDAD DE GEL DE ELECTROFORESIS. ESTE METODO RESULTA ESPECIALMENTE UTIL PARA PROPORCIONAR UN ENSAYO DE COMPONENTES MULTIPLES, EXTREMADAMENTE SENSIBLE,, UTILIZANDO POLIPEPTIDOS ANTIGENICOS ESENCIALMENTE PUROS, CAPACES DE DETECTAR LA PRESENCIA DE ANTICUERPOS A LOS VIRUS HIV-1 O HIV-2 (DEL SIDA) EN PACIENTES INFECTADOS.
ES90108710T 1989-05-09 1990-05-09 Procedimiento para preparar un ensayo inmunologico de transferencia western. Expired - Lifetime ES2091777T3 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US35018089A 1989-05-09 1989-05-09

Publications (1)

Publication Number Publication Date
ES2091777T3 true ES2091777T3 (es) 1996-11-16

Family

ID=23375548

Family Applications (1)

Application Number Title Priority Date Filing Date
ES90108710T Expired - Lifetime ES2091777T3 (es) 1989-05-09 1990-05-09 Procedimiento para preparar un ensayo inmunologico de transferencia western.

Country Status (9)

Country Link
US (1) US5356772A (es)
EP (1) EP0397129B1 (es)
JP (1) JP2945074B2 (es)
KR (1) KR900018680A (es)
AT (1) ATE140540T1 (es)
AU (1) AU637097B2 (es)
CA (1) CA2016313C (es)
DE (1) DE69027812T2 (es)
ES (1) ES2091777T3 (es)

Families Citing this family (28)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5120662A (en) * 1989-05-09 1992-06-09 Abbott Laboratories Multilayer solid phase immunoassay support and method of use
EP0473065A3 (en) * 1990-08-29 1992-08-26 Abbott Laboratories Simultaneous assay for detecting two or more analytes
WO1994027150A1 (fr) * 1993-05-10 1994-11-24 Nissui Pharmaceutical Co., Ltd. Procede de dosage de plus d'un ligand immunologique, reactif de dosage et kit utilise a cet effet
EP0627625A1 (en) * 1993-06-04 1994-12-07 Abbott Laboratories Immunoassay for differential diagnosis
US5731161A (en) * 1995-04-24 1998-03-24 Allergan, Inc. Botulinum toxin antibody detection assay
US6020122A (en) * 1995-06-07 2000-02-01 Abbott Laboratories Hepatitis C virus second envelope (HCV-E2) glycoprotein expression system
IT1290804B1 (it) 1996-08-16 1998-12-11 Abbott Lab Saggio western blot perfezionato per l'individuazione di anticorpi specifici contro il virus hcmv
US6680208B1 (en) 1999-11-19 2004-01-20 Becton, Dickinson And Company Rapid protein identification using antibody mixtures
US6933109B2 (en) * 2000-12-22 2005-08-23 Large Scale Proteomics Corporation Rapid particle detection
EP1853696B1 (en) 2005-02-24 2019-04-17 Life Technologies (Israel) Ltd. Electro-blotting devices, systems, and kits, and methods for their use
US7749717B2 (en) * 2006-10-19 2010-07-06 Abbott Laboratories Methods for the detection and diagnosis of Trypanosoma cruzi infection
US8075755B2 (en) * 2007-12-13 2011-12-13 Bio-Rad Laboratories, Inc. Polymeric sorbent sheets as ion reservoirs for electroblotting
JP6058263B2 (ja) 2008-04-11 2017-01-11 バーグ リミテッド ライアビリティ カンパニー 癌細胞においてアポトーシスを誘導する方法および使用
CN102150045A (zh) 2008-07-11 2011-08-10 生命技术公司 增强型电泳检测固相支持物上的分析物
US8715476B2 (en) 2009-12-11 2014-05-06 Bio-Rad Laboratories, Inc. Instrument for independent electrotransfer in multiple cassettes
WO2011119920A2 (en) 2010-03-25 2011-09-29 Oregon Health & Science University Cmv glycoproteins and recombinant vectors
ES2667425T3 (es) 2011-06-10 2018-05-10 Oregon Health & Science University Glucoproteínas y vectores recombinantes de CMV
AU2012216792A1 (en) 2011-09-12 2013-03-28 International Aids Vaccine Initiative Immunoselection of recombinant vesicular stomatitis virus expressing HIV-1 proteins by broadly neutralizing antibodies
EP2586461A1 (en) 2011-10-27 2013-05-01 Christopher L. Parks Viral particles derived from an enveloped virus
EP2679596B1 (en) 2012-06-27 2017-04-12 International Aids Vaccine Initiative HIV-1 env glycoprotein variant
WO2014018721A1 (en) 2012-07-25 2014-01-30 Bio-Rad Laboratories, Inc. Targeted delivery of reagents through patterned transfer sheets
WO2014062885A1 (en) 2012-10-17 2014-04-24 Bio-Rad Laboratories, Inc. Image capture for large analyte arrays
US9291596B2 (en) 2013-02-12 2016-03-22 Pierce Biotechnology, Inc. Electroblot transfer buffer
USD738527S1 (en) 2013-05-28 2015-09-08 Life Technologies Corporation Electroblotting apparatus
US20150065381A1 (en) 2013-09-05 2015-03-05 International Aids Vaccine Initiative Methods of identifying novel hiv-1 immunogens
EP2873423B1 (en) 2013-10-07 2017-05-31 International Aids Vaccine Initiative Soluble hiv-1 envelope glycoprotein trimers
US10174292B2 (en) 2015-03-20 2019-01-08 International Aids Vaccine Initiative Soluble HIV-1 envelope glycoprotein trimers
EP3072901A1 (en) 2015-03-23 2016-09-28 International Aids Vaccine Initiative Soluble hiv-1 envelope glycoprotein trimers

Family Cites Families (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4452901A (en) * 1980-03-20 1984-06-05 Ciba-Geigy Corporation Electrophoretically transferring electropherograms to nitrocellulose sheets for immuno-assays
EP0063810B1 (en) * 1981-04-29 1986-03-05 Ciba-Geigy Ag New devices and kits for immunological analysis
NZ228756A (en) * 1984-04-23 1990-10-26 Us Health Detection of hiv antigen using competition immune assay or western blot assay
ZA855031B (en) * 1984-08-31 1986-02-26 New York Blood Center Inc Assay for simultaneous detection of antigen and antibody in given serum
US4843011A (en) * 1985-08-01 1989-06-27 Akzo N.V. Monoclonal antibodies for binding HTLV-III proteins, and cell lines for their production
CH669265A5 (de) * 1985-12-18 1989-02-28 Celldynamics Ag Multiindikator-teststreifen fuer immunologische analysen, ihre herstellung und verwendung.
US4980279A (en) * 1986-04-15 1990-12-25 Scripps Clinic And Research Foundation Cellular fibronectin: polypeptides, anti-polypeptide antibodies and assay methods
US4816387A (en) * 1986-06-06 1989-03-28 Bio-Research Laboratories, Inc. Method for detection of antibodies to HTLV-III and diagnostic test kit useful therewith
DE3854665T2 (de) * 1987-07-13 1996-03-21 Verigen Inc Verfahren zum schnellen und empfindlichen nachweis von hiv-1-antikörpern.
JPH01113663A (ja) * 1987-10-28 1989-05-02 Eisai Co Ltd Ａｔｌｖ抗体の測定試薬および測定方法

Also Published As

Publication number Publication date
JPH0328765A (ja) 1991-02-06
CA2016313A1 (en) 1990-11-09
EP0397129A3 (en) 1991-11-13
DE69027812T2 (de) 1997-02-13
KR900018680A (ko) 1990-12-22
ATE140540T1 (de) 1996-08-15
CA2016313C (en) 2002-12-31
EP0397129B1 (en) 1996-07-17
US5356772A (en) 1994-10-18
JP2945074B2 (ja) 1999-09-06
AU5474290A (en) 1990-11-15
AU637097B2 (en) 1993-05-20
EP0397129A2 (en) 1990-11-14
DE69027812D1 (de) 1996-08-22

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