ES2091876T3 - Metodo de produccion de un polinucleotido para uso en amplificacion con un unico cebador. - Google Patents

Metodo de produccion de un polinucleotido para uso en amplificacion con un unico cebador.

Info

Publication number
ES2091876T3
ES2091876T3 ES91306550T ES91306550T ES2091876T3 ES 2091876 T3 ES2091876 T3 ES 2091876T3 ES 91306550 T ES91306550 T ES 91306550T ES 91306550 T ES91306550 T ES 91306550T ES 2091876 T3 ES2091876 T3 ES 2091876T3
Authority
ES
Spain
Prior art keywords
extended
primer
polynucleotide
extender probe
duplex
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
ES91306550T
Other languages
English (en)
Inventor
Samuel Rose
Linda M Western
Martin Becker
Edwin F Ullman
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Siemens Healthcare Diagnostics GmbH Germany
Original Assignee
Behringwerke AG
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Behringwerke AG filed Critical Behringwerke AG
Application granted granted Critical
Publication of ES2091876T3 publication Critical patent/ES2091876T3/es
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6853—Nucleic acid amplification reactions using modified primers or templates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/686—Polymerase chain reaction [PCR]

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Biomedical Technology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Plant Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)

Abstract

SE DESCRIBE UN METODO PARA PRODUCIR UN POLIDEOXINUCLEOTIDO DE UNA SOLA HEBRA TENIENDO DOS SEGMENTOS QUE NO SON CONTINUOS Y COMPLEMENTARIOS UNO CON OTRO. EL METODO COMPRENDE LOS PASOS DE PROPORCIONAR EN COMBINACION (1) UN POLINUCLEOTIDO TENIENDO DOS SECUENCIAS NUCLEOTIDAS NO CONTINUAS NI COMPLEMENTARIAS S1 Y S2 DONDE S2 ES 5'' DE S1 Y ES AL MENOS DE UNA LONGITUD DE DIEZ DEOXINUCLEOTIDOS Y (2) UNA PRUEBA DE EXTENSION COMPUESTA DE DOS SECUENCIAS DEOXINUCLEOTIDAS, DONDE LA SECUENCIA EN EL EXTREMO 3'' DE LA PRUEBA DE EXTENSION ES HIBRIDIZABLE CON S1 Y EL OTRO DE LA SECUENCIA DEOXINUCLEOTIDO ES HOMOLOGO A S2 Y (B) EXTENDIENDO LA PRUEBA DE EXTENSION A LO LARGO DEL POLINUCLEOTIDO. EL METODO PUEDE COMPRENDER TAMBIEN PROVISTO EN LA COMBINACION UN PRIMER POLIDEOXINUCLEOTIDO CAPAZ DE HIBRIDIZAR AL MENOS EN SU EXTREMO-3'' CON UNA SECUENCIA NUCLEOTIDA COMPLEMENTARIA A S2 BAJO CONDICIONES DONDE (1) LA PRUEBA DE EXTENSION EXTENDIDA SE VUELVE DE UNA SOLA HEBRA, (2) EL PRIMER POLIDEOXINUCLEOTIDO HIBRIDIZA CON,Y SE EXTIENDE A LO LARGO DE LA PRUEBA DE EXTENSION EXTENDIDA PARA FORMAR UN DUPLEX COMPRENDIENDO EL PRIMER EXTENDIDO, (3) EL PRIMER EXTENDIDO SE DISOCIA DEL DUPLEX, Y (4) EL PRIMERO HIBRIDIZA CON Y SE EXTIENDE A LO LARGO DEL PRIMER EXTENDIDO PARA FORMAR UN DUPLEX COMPRENDIENDO EL PRIMER EXTENDIDO, Y REPITIENDO LOS PASOS (3) Y (4). EL METODO ENCUENTRA UNAS APLICACIONES PARTICULARES EN LA DETECCION DE ANALITICOS POLINUCLEOTIDOS.
ES91306550T 1990-07-19 1991-07-18 Metodo de produccion de un polinucleotido para uso en amplificacion con un unico cebador. Expired - Lifetime ES2091876T3 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US07/555,323 US5595891A (en) 1990-07-19 1990-07-19 Method for producing a polynucleotide for use in single primer amplification

Publications (1)

Publication Number Publication Date
ES2091876T3 true ES2091876T3 (es) 1996-11-16

Family

ID=24216831

Family Applications (1)

Application Number Title Priority Date Filing Date
ES91306550T Expired - Lifetime ES2091876T3 (es) 1990-07-19 1991-07-18 Metodo de produccion de un polinucleotido para uso en amplificacion con un unico cebador.

Country Status (7)

Country Link
US (1) US5595891A (es)
EP (1) EP0469755B1 (es)
JP (1) JPH0584079A (es)
AT (1) ATE143697T1 (es)
CA (1) CA2047342A1 (es)
DE (1) DE69122457T2 (es)
ES (1) ES2091876T3 (es)

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ZA929319B (en) * 1991-12-11 1993-05-24 Igen Inc Method for exponential amplification of nucleic acid by a single unpaired primer.
AU3728093A (en) * 1992-02-19 1993-09-13 Public Health Research Institute Of The City Of New York, Inc., The Novel oligonucleotide arrays and their use for sorting, isolating, sequencing, and manipulating nucleic acids
GB9210176D0 (en) † 1992-05-12 1992-06-24 Cemu Bioteknik Ab Chemical method
GB9210177D0 (en) * 1992-05-12 1992-06-24 Cemu Bioteknik Ab Loop structures
ATE152180T1 (de) * 1992-07-31 1997-05-15 Behringwerke Ag Verfahren zur einführung von definierten sequenzen am 3' ende von polynukleotiden
WO1994003624A1 (en) * 1992-08-04 1994-02-17 Auerbach Jeffrey I Methods for the isothermal amplification of nucleic acid molecules
US6261808B1 (en) * 1992-08-04 2001-07-17 Replicon, Inc. Amplification of nucleic acid molecules via circular replicons
US6294323B1 (en) * 1993-04-14 2001-09-25 Behringwerke Ag Self initiating single primer amplification of nucleic acids
US5470723A (en) * 1993-05-05 1995-11-28 Becton, Dickinson And Company Detection of mycobacteria by multiplex nucleic acid amplification
US5422252A (en) * 1993-06-04 1995-06-06 Becton, Dickinson And Company Simultaneous amplification of multiple targets
US5681697A (en) * 1993-12-08 1997-10-28 Chiron Corporation Solution phase nucleic acid sandwich assays having reduced background noise and kits therefor
US5710000A (en) * 1994-09-16 1998-01-20 Affymetrix, Inc. Capturing sequences adjacent to Type-IIs restriction sites for genomic library mapping
WO1997004131A1 (en) * 1995-07-21 1997-02-06 Forsyth Dental Infirmary For Children Single primer amplification of polynucleotide hairpins
ES2279530T3 (es) * 1995-11-25 2007-08-16 Roche Diagnostics Gmbh Metodo para generar multiples acidos nucleicos bicatenarios.
WO1997023646A1 (en) 1995-12-22 1997-07-03 Behringwerke Aktiengesellschaft Detection of differences in nucleic acids
US6326143B1 (en) 1996-11-22 2001-12-04 Roche Diagnostics Gmbh Method for generating multiple double stranded nucleic acids
US8445664B2 (en) * 1998-06-24 2013-05-21 Enzo Diagnostics, Inc. Kits for amplifying and detecting nucleic acid sequences
US6743605B1 (en) * 1998-06-24 2004-06-01 Enzo Life Sciences, Inc. Linear amplification of specific nucleic acid sequences
US6410278B1 (en) * 1998-11-09 2002-06-25 Eiken Kagaku Kabushiki Kaisha Process for synthesizing nucleic acid
US6232104B1 (en) 1999-08-17 2001-05-15 Dade Behring Inc. Detection of differences in nucleic acids by inhibition of spontaneous DNA branch migration
US6692918B2 (en) 1999-09-13 2004-02-17 Nugen Technologies, Inc. Methods and compositions for linear isothermal amplification of polynucleotide sequences
CA2384838C (en) 1999-09-13 2006-07-18 Nugen Technologies, Inc. Methods and compositions for linear isothermal amplification of polynucleotide sequences
JP4721603B2 (ja) * 1999-11-08 2011-07-13 栄研化学株式会社 変異および／または多型の検出方法
WO2001034838A1 (en) 1999-11-08 2001-05-17 Eiken Kagaku Kabushiki Kaisha Method of detecting variation or polymorphism
EP2253717B1 (en) * 2000-04-07 2014-04-30 Eiken Kagaku Kabushiki Kaisha Method of amplifying nucleic acid by using double-stranded nucleic acid as template
US7846733B2 (en) * 2000-06-26 2010-12-07 Nugen Technologies, Inc. Methods and compositions for transcription-based nucleic acid amplification
ATE455186T1 (de) * 2000-06-26 2010-01-15 Nugen Technologies Inc Methoden und zusammensetzungen zur auf transkription basierenden vervielfältigung von nukleinsäuren
WO2002048401A2 (en) 2000-12-11 2002-06-20 Alexion Pharmaceuticals, Inc. Nested oligonucleotides containing hairpin for nucleic acid amplification
WO2002048402A2 (en) 2000-12-13 2002-06-20 Nugen Technologies, Inc. Methods and compositions for generation of multiple copies of nucleic acid sequences and methods of detection thereof
CA2439074A1 (en) * 2001-03-09 2002-09-19 Nugen Technologies, Inc. Methods and compositions for amplification of rna sequences
KR20030082535A (ko) * 2001-03-09 2003-10-22 뉴젠 테크놀로지스 인코포레이티드 Ｒｎａ 서열의 증폭을 위한 방법 및 조성물
ATE448320T1 (de) * 2001-09-19 2009-11-15 Alexion Pharma Inc Manipulierte matrizen und ihre verwendung bei der single-primer-amplifikation
US7414111B2 (en) * 2001-09-19 2008-08-19 Alexion Pharmaceuticals, Inc. Engineered templates and their use in single primer amplification
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CA2012982A1 (en) * 1989-03-27 1990-09-27 Frank W. Hobbs Process for rapid nucleic acid detection by incorporating a reporter moiety into amplified target nucleic acid

Also Published As

Publication number Publication date
US5595891A (en) 1997-01-21
ATE143697T1 (de) 1996-10-15
EP0469755B1 (en) 1996-10-02
DE69122457T2 (de) 1997-03-20
EP0469755A1 (en) 1992-02-05
CA2047342A1 (en) 1992-01-20
DE69122457D1 (de) 1996-11-07
JPH0584079A (ja) 1993-04-06

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