ES2091876T3 - Metodo de produccion de un polinucleotido para uso en amplificacion con un unico cebador. - Google Patents

Metodo de produccion de un polinucleotido para uso en amplificacion con un unico cebador.

Info

Publication number
ES2091876T3
ES2091876T3 ES91306550T ES91306550T ES2091876T3 ES 2091876 T3 ES2091876 T3 ES 2091876T3 ES 91306550 T ES91306550 T ES 91306550T ES 91306550 T ES91306550 T ES 91306550T ES 2091876 T3 ES2091876 T3 ES 2091876T3
Authority
ES
Spain
Prior art keywords
extended
primer
polynucleotide
extender probe
duplex
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
ES91306550T
Other languages
English (en)
Inventor
Samuel Rose
Linda M Western
Martin Becker
Edwin F Ullman
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Siemens Healthcare Diagnostics GmbH Germany
Original Assignee
Behringwerke AG
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Behringwerke AG filed Critical Behringwerke AG
Application granted granted Critical
Publication of ES2091876T3 publication Critical patent/ES2091876T3/es
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6853—Nucleic acid amplification reactions using modified primers or templates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/686—Polymerase chain reaction [PCR]

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Biomedical Technology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Plant Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)

Abstract

SE DESCRIBE UN METODO PARA PRODUCIR UN POLIDEOXINUCLEOTIDO DE UNA SOLA HEBRA TENIENDO DOS SEGMENTOS QUE NO SON CONTINUOS Y COMPLEMENTARIOS UNO CON OTRO. EL METODO COMPRENDE LOS PASOS DE PROPORCIONAR EN COMBINACION (1) UN POLINUCLEOTIDO TENIENDO DOS SECUENCIAS NUCLEOTIDAS NO CONTINUAS NI COMPLEMENTARIAS S1 Y S2 DONDE S2 ES 5'' DE S1 Y ES AL MENOS DE UNA LONGITUD DE DIEZ DEOXINUCLEOTIDOS Y (2) UNA PRUEBA DE EXTENSION COMPUESTA DE DOS SECUENCIAS DEOXINUCLEOTIDAS, DONDE LA SECUENCIA EN EL EXTREMO 3'' DE LA PRUEBA DE EXTENSION ES HIBRIDIZABLE CON S1 Y EL OTRO DE LA SECUENCIA DEOXINUCLEOTIDO ES HOMOLOGO A S2 Y (B) EXTENDIENDO LA PRUEBA DE EXTENSION A LO LARGO DEL POLINUCLEOTIDO. EL METODO PUEDE COMPRENDER TAMBIEN PROVISTO EN LA COMBINACION UN PRIMER POLIDEOXINUCLEOTIDO CAPAZ DE HIBRIDIZAR AL MENOS EN SU EXTREMO-3'' CON UNA SECUENCIA NUCLEOTIDA COMPLEMENTARIA A S2 BAJO CONDICIONES DONDE (1) LA PRUEBA DE EXTENSION EXTENDIDA SE VUELVE DE UNA SOLA HEBRA, (2) EL PRIMER POLIDEOXINUCLEOTIDO HIBRIDIZA CON,Y SE EXTIENDE A LO LARGO DE LA PRUEBA DE EXTENSION EXTENDIDA PARA FORMAR UN DUPLEX COMPRENDIENDO EL PRIMER EXTENDIDO, (3) EL PRIMER EXTENDIDO SE DISOCIA DEL DUPLEX, Y (4) EL PRIMERO HIBRIDIZA CON Y SE EXTIENDE A LO LARGO DEL PRIMER EXTENDIDO PARA FORMAR UN DUPLEX COMPRENDIENDO EL PRIMER EXTENDIDO, Y REPITIENDO LOS PASOS (3) Y (4). EL METODO ENCUENTRA UNAS APLICACIONES PARTICULARES EN LA DETECCION DE ANALITICOS POLINUCLEOTIDOS.
ES91306550T 1990-07-19 1991-07-18 Metodo de produccion de un polinucleotido para uso en amplificacion con un unico cebador. Expired - Lifetime ES2091876T3 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US07/555,323 US5595891A (en) 1990-07-19 1990-07-19 Method for producing a polynucleotide for use in single primer amplification

Publications (1)

Publication Number Publication Date
ES2091876T3 true ES2091876T3 (es) 1996-11-16

Family

ID=24216831

Family Applications (1)

Application Number Title Priority Date Filing Date
ES91306550T Expired - Lifetime ES2091876T3 (es) 1990-07-19 1991-07-18 Metodo de produccion de un polinucleotido para uso en amplificacion con un unico cebador.

Country Status (7)

Country Link
US (1) US5595891A (es)
EP (1) EP0469755B1 (es)
JP (1) JPH0584079A (es)
AT (1) ATE143697T1 (es)
CA (1) CA2047342A1 (es)
DE (1) DE69122457T2 (es)
ES (1) ES2091876T3 (es)

Families Citing this family (52)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5525462A (en) * 1991-05-02 1996-06-11 Toyo Boseki Kabushiki Kaisha Nucleic acid sequence amplification method, detection method, and reagent kit therefor
CA2080305A1 (en) * 1991-10-11 1993-04-12 Linda M. Western Method for producing a polynucleotide for use in single primer amplification and phosphorothioate-containing oligonucleotides as primers in nucleic acid amplification
ZA929319B (en) * 1991-12-11 1993-05-24 Igen Inc Method for exponential amplification of nucleic acid by a single unpaired primer.
WO1993017126A1 (en) * 1992-02-19 1993-09-02 The Public Health Research Institute Of The City Of New York, Inc. Novel oligonucleotide arrays and their use for sorting, isolating, sequencing, and manipulating nucleic acids
GB9210176D0 (en) * 1992-05-12 1992-06-24 Cemu Bioteknik Ab Chemical method
GB9210177D0 (en) * 1992-05-12 1992-06-24 Cemu Bioteknik Ab Loop structures
DE69310179T2 (de) * 1992-07-31 1997-07-31 Behringwerke Ag Verfahren zur einführung von definierten sequenzen am 3' ende von polynukleotiden
WO1994003624A1 (en) * 1992-08-04 1994-02-17 Auerbach Jeffrey I Methods for the isothermal amplification of nucleic acid molecules
US6261808B1 (en) * 1992-08-04 2001-07-17 Replicon, Inc. Amplification of nucleic acid molecules via circular replicons
US6294323B1 (en) * 1993-04-14 2001-09-25 Behringwerke Ag Self initiating single primer amplification of nucleic acids
US5422252A (en) * 1993-06-04 1995-06-06 Becton, Dickinson And Company Simultaneous amplification of multiple targets
US5470723A (en) * 1993-05-05 1995-11-28 Becton, Dickinson And Company Detection of mycobacteria by multiplex nucleic acid amplification
US5681697A (en) * 1993-12-08 1997-10-28 Chiron Corporation Solution phase nucleic acid sandwich assays having reduced background noise and kits therefor
US5710000A (en) * 1994-09-16 1998-01-20 Affymetrix, Inc. Capturing sequences adjacent to Type-IIs restriction sites for genomic library mapping
WO1997004131A1 (en) * 1995-07-21 1997-02-06 Forsyth Dental Infirmary For Children Single primer amplification of polynucleotide hairpins
JP2000500976A (ja) * 1995-11-25 2000-02-02 ベーリンガー マンハイム ゲーエムベーハー 多数の二本鎖核酸の生成方法
AU1565397A (en) * 1995-12-22 1997-07-17 Behringwerke Aktiengesellschaft Detection of differences in nucleic acids
US6326143B1 (en) 1996-11-22 2001-12-04 Roche Diagnostics Gmbh Method for generating multiple double stranded nucleic acids
US8445664B2 (en) * 1998-06-24 2013-05-21 Enzo Diagnostics, Inc. Kits for amplifying and detecting nucleic acid sequences
US6743605B1 (en) * 1998-06-24 2004-06-01 Enzo Life Sciences, Inc. Linear amplification of specific nucleic acid sequences
ATE374833T1 (de) 1998-11-09 2007-10-15 Eiken Chemical Verfahren zur synthese von nukleinsäuren
US6232104B1 (en) 1999-08-17 2001-05-15 Dade Behring Inc. Detection of differences in nucleic acids by inhibition of spontaneous DNA branch migration
MXPA02002656A (es) 1999-09-13 2003-10-14 Nugen Technologies Inc Metodos y composiciones para la ampliacion lineal isotermica de secuencias de polinucleotidos.
US6692918B2 (en) * 1999-09-13 2004-02-17 Nugen Technologies, Inc. Methods and compositions for linear isothermal amplification of polynucleotide sequences
EP1231281B1 (en) 1999-11-08 2006-02-01 Eiken Kagaku Kabushiki Kaisha Method of detecting variation or polymorphism
HK1052203A1 (zh) * 1999-11-08 2003-09-05 荣研化学株式会社 检测突变和/或多态性的方法
ES2546450T3 (es) * 2000-04-07 2015-09-23 Eiken Kagaku Kabushiki Kaisha Método para amplificar y detectar un ácido nucleico usando ácido nucleico bicatenario como molde
US7846733B2 (en) * 2000-06-26 2010-12-07 Nugen Technologies, Inc. Methods and compositions for transcription-based nucleic acid amplification
EP1356094B1 (en) * 2000-06-26 2010-01-13 Nugen Technologies, Inc. Methods and compositions for transcription-based nucleic acid amplification
AU2002230734A1 (en) 2000-12-11 2002-06-24 Alexion Pharmaceuticals, Inc. Nested oligonucleotides containing hairpin for nucleic acid amplification
WO2002048402A2 (en) 2000-12-13 2002-06-20 Nugen Technologies, Inc. Methods and compositions for generation of multiple copies of nucleic acid sequences and methods of detection thereof
WO2002072773A2 (en) * 2001-03-09 2002-09-19 Nugen Technologies, Inc. Methods and compositions for amplification of rna sequences
JP4542312B2 (ja) 2001-03-09 2010-09-15 ニューゲン テクノロジーズ， インコーポレイテッド Ｒｎａ配列の増幅のための方法および組成物
US7414111B2 (en) * 2001-09-19 2008-08-19 Alexion Pharmaceuticals, Inc. Engineered templates and their use in single primer amplification
US7306906B2 (en) * 2001-09-19 2007-12-11 Alexion Pharmaceuticals, Inc. Engineered templates and their use in single primer amplification
US7045319B2 (en) * 2001-10-30 2006-05-16 Ribomed Biotechnologies, Inc. Molecular detection systems utilizing reiterative oligonucleotide synthesis
US20040054162A1 (en) * 2001-10-30 2004-03-18 Hanna Michelle M. Molecular detection systems utilizing reiterative oligonucleotide synthesis
US20110151438A9 (en) 2001-11-19 2011-06-23 Affymetrix, Inc. Methods of Analysis of Methylation
AU2002366098A1 (en) * 2001-11-19 2003-06-10 Parallele Bioscience Inc Multiplex pcr
GB0218215D0 (en) * 2002-08-06 2002-09-11 Tepnel Medical Ltd Amplification of nucleic acids
JP2006523465A (ja) 2003-04-14 2006-10-19 ニューゲン テクノロジーズ， インコーポレイテッド ランダムにプライミングされる複合プライマーを用いる大規模増幅
WO2005042709A2 (en) * 2003-10-29 2005-05-12 Ribomed Biotechnologies, Inc. Compositions, methods and detection technologies for reiterative oligonucleotide synthesis
WO2007030759A2 (en) * 2005-09-07 2007-03-15 Nugen Technologies, Inc. Improved nucleic acid amplification procedure
US8137936B2 (en) * 2005-11-29 2012-03-20 Macevicz Stephen C Selected amplification of polynucleotides
US20090203531A1 (en) * 2008-02-12 2009-08-13 Nurith Kurn Method for Archiving and Clonal Expansion
GB2470672B (en) 2008-03-21 2012-09-12 Nugen Technologies Inc Methods of RNA amplification in the presence of DNA
CA2724343A1 (en) 2008-05-15 2009-11-19 Ribomed Biotechnologies, Inc. Methods and reagents for detecting cpg methylation with a methyl cpg binding protein (mbp)
WO2010009060A2 (en) * 2008-07-13 2010-01-21 Ribomed Biotechnologies, Inc. Molecular beacon-based methods for detection of targets using abscription
RU2011141720A (ru) 2009-03-15 2013-04-27 Рибомед Байотекнолоджиз, Инк. Молекулярное обнаружение, основанное на абскрипции
CA2686250C (en) 2009-11-12 2016-10-11 Fountainhead, Llc Floating treatment streambed
CN105026573A (zh) 2012-11-28 2015-11-04 艾布维兹生物公司 用于单引物扩增的基因特异模板的制备
WO2017106731A1 (en) 2015-12-18 2017-06-22 Monsanto Technology Llc Methods for producing corn plants with northern leaf blight resistance and compositions thereof

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4683195A (en) * 1986-01-30 1987-07-28 Cetus Corporation Process for amplifying, detecting, and/or-cloning nucleic acid sequences
US4868104A (en) * 1985-09-06 1989-09-19 Syntex (U.S.A.) Inc. Homogeneous assay for specific polynucleotides
US5066584A (en) * 1988-09-23 1991-11-19 Cetus Corporation Methods for generating single stranded dna by the polymerase chain reaction
US5508178A (en) * 1989-01-19 1996-04-16 Rose; Samuel Nucleic acid amplification using single primer
ATE142272T1 (de) * 1989-01-19 1996-09-15 Behringwerke Ag Nukleinsäure-amplifikation unter verwendung eines einzelprimers
CA2012982A1 (en) * 1989-03-27 1990-09-27 Frank W. Hobbs Process for rapid nucleic acid detection by incorporating a reporter moiety into amplified target nucleic acid

Also Published As

Publication number Publication date
US5595891A (en) 1997-01-21
DE69122457D1 (de) 1996-11-07
EP0469755A1 (en) 1992-02-05
DE69122457T2 (de) 1997-03-20
JPH0584079A (ja) 1993-04-06
ATE143697T1 (de) 1996-10-15
EP0469755B1 (en) 1996-10-02
CA2047342A1 (en) 1992-01-20

Similar Documents

Publication Publication Date Title
ES2091876T3 (es) Metodo de produccion de un polinucleotido para uso en amplificacion con un unico cebador.
ES2112302T3 (es) Metodo para producir un polinucleotido para uso en amplificacion con un unico cebador y oligonucleotidos que contienen fosforotioato como cebadores para la amplificacion de acidos nucleicos.
ATE114651T1 (de) Alkynylamino-nukleotide.
ATE121416T1 (de) Oligonukleotid-analoge.
ATE152180T1 (de) Verfahren zur einführung von definierten sequenzen am 3' ende von polynukleotiden
ES2093633T3 (es) Amplificacion de acidos nucleicos utilizando un unico cebador.
DE3885027D1 (de) Amplifikationsverfahren zum Nachweis von Polynukleotide.
FI863965A0 (fi) Foerfarande foer styrning av en anlaeggning saosom dammsugare, centraldammsugare, maskinellt luftkonditioneringssystem eller motsvarande.
DE3776966D1 (de) Funktionelle gruppen tragende liposome und verfahren zu deren herstellung.
DE3785632D1 (de) Verfahren zur herstellung von kornorientierten silizium-stahlblechen mit sehr niedrigen walzverlusten.
ES8609472A1 (es) Una sonda de deteccion de acidos nucleicos
DE3777603D1 (de) Verfahren zur herstellung einer halbleiteranordnung mit einem halbleitersubstrat, das feldoxidzonen an seiner oberflaeche enthaelt.
HUP9802918A2 (hu) Eljárás skizofrénia diagnosztizálására
IT1232975B (it) Differenziale con anelli di stacco sfalsati angolarmente.
DE3774035D1 (de) Verfahren zur herstellung von pech, verwertbar zur herstellung von kohlenstoffkoerpern.
DE3750087D1 (de) Dekorblatt, sowie verfahren zur herstellung desselben.
DE3787998D1 (de) ART-Nukleotidsegmente, -Vektoren, -Zellinien, Verfahren zur Herstellung und Verwendung.
FI874631A0 (fi) Arrangemang vid lindragningsanordning anordnad i aendan av en upplaeggnings- och/eller transportlinje.
DE3764691D1 (de) Mesomorphe polymere mit seitenketten mit hoher dielektrischer anisotropie und verfahren zu deren herstellung.
NO870612D0 (no) Enkel hybridiseringsanalyse i opploesningsfase for deteksjon av polynukleotidsekvenser.
ES2093688T3 (es) Deteccion especifica del mycobacterium tuberculosis.
AU6932991A (en) Probability estimation based on decision history
DE68903915D1 (de) Verfahren zur herstellung von diorganopolysiloxan-gruppen enthaltenden thermostabilen copolyimidamiden.
FI874057A0 (fi) Anvaendning av manganoxidkonkretioner eller manganhaltiga djuphavsmalmer i katalysatorer foer minskning av kvaeveoxider i avgaser.
DE3778329D1 (de) Verfahren zur herstellung von 2,6-dichlorobenzonitril.

Legal Events

Date Code Title Description
FG2A Definitive protection

Ref document number: 469755

Country of ref document: ES