ES2093633T3 - Amplificacion de acidos nucleicos utilizando un unico cebador. - Google Patents

Amplificacion de acidos nucleicos utilizando un unico cebador.

Info

Publication number
ES2093633T3
ES2093633T3 ES90300528T ES90300528T ES2093633T3 ES 2093633 T3 ES2093633 T3 ES 2093633T3 ES 90300528 T ES90300528 T ES 90300528T ES 90300528 T ES90300528 T ES 90300528T ES 2093633 T3 ES2093633 T3 ES 2093633T3
Authority
ES
Spain
Prior art keywords
polynucleotide
single stranded
sequence
analyte
stranded polynucleotide
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
ES90300528T
Other languages
English (en)
Inventor
Samuel Rose
Thomas C Goodman
Linda M Western
Martin Becker
Edwin F Ullman
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Siemens Healthcare Diagnostics GmbH Germany
Original Assignee
Behringwerke AG
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Behringwerke AG filed Critical Behringwerke AG
Application granted granted Critical
Publication of ES2093633T3 publication Critical patent/ES2093633T3/es
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/682—Signal amplification
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6853—Nucleic acid amplification reactions using modified primers or templates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6862—Ligase chain reaction [LCR]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • C12Q1/689—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for bacteria

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Biotechnology (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
  • Investigating Or Analysing Biological Materials (AREA)

Abstract

SE DESCUBRE UN METODO PARA DETERMINAR LA PRESENCIA DE UN INDICADOR POLINUCLEOTIDO EN UNA MUESTRA SUSCEPTIBLE DE CONTENER TAL INDICADOR. EL METODO COMPRENDE (A) FORMACION COMO RESULTADO DE LA PRESENCIA DE UN INDICADOR DE UN POLINUCLEOTIDO SENCILLO QUE COMPRENDE UNA SECUENCIA DE ENLACE DEL POLINUCLEOTIDO OBJETO FLANQUEADO POR UNA PRIMERA Y SEGUNDA SECUENCIAS DE POLINUCLEOTIDOS QUE DIFIEREN DE LA SECUENCIA DEL INDICADOR O UNA SECUENCIA COMPLEMENTARIA DE LA SECUENCIA DEL INDICADOR (B) FORMACION DE MULTIPLES COPIAS DEL POLINUCLEOTIDO SENCILLO, Y (C) DETECCION DEL POLINUCLEOTIDO SENCILLO. TAMBIEN SE DESCUBRE UN METODO PARA PRODUCIR AL MENOS UNA COPIA DE UN POLINUCLEOTIDO SENCILLO. EL METODO COMPRENDE (A) FORMACION EN PRESENCIA DE TRIFOSFATOS, NUCLEOTIDOS Y POLIMERASA POLINUCLEOTIDA DEPENDIENTE DE UNA EXTENSION DE UN POLINUCLEOTIDO IMPRIMADOR DE AL MENOS LOS 3'' EXTREMOS DE LOS CUALES TIENE AL MENOS UNA SECUENCIA DE BASE 10 HIDRIZABLE CON UNA SEGUNDA SECUENCIA FLANQUEANDO LOS 3'' EXTREMOS DEL POLINUCLEOTIDO SENCILLO, SIENDO LA SEGUNDA SECUENCIA PARCIAL O TOTALMENTE COMPLEMENTARIA CON AL MENOS UNA PRIMERA SECUENCIA DE BASE 10 FLANQUEANDO LOS 5'' EXTREMOS DEL POLINUCLEOTIDO SENCILLO, (B) DISOCIACION DEL IMPRIMADOR POLINUCLEOTIDO EXTENDIDO Y DEL POLINUCLEOTIDO SENCILLO, (C) REPETICION DEL PASO (A) Y (D) DISOCIACION DEL POLINUCLEOTIDO IMPRIMADOR EXTENDIDO Y DE LA COPIA DEL POLINUCLEOTIDO SENCILLO.
ES90300528T 1989-01-19 1990-01-18 Amplificacion de acidos nucleicos utilizando un unico cebador. Expired - Lifetime ES2093633T3 (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US29928289A 1989-01-19 1989-01-19
US39979589A 1989-08-29 1989-08-29

Publications (1)

Publication Number Publication Date
ES2093633T3 true ES2093633T3 (es) 1997-01-01

Family

ID=26971136

Family Applications (1)

Application Number Title Priority Date Filing Date
ES90300528T Expired - Lifetime ES2093633T3 (es) 1989-01-19 1990-01-18 Amplificacion de acidos nucleicos utilizando un unico cebador.

Country Status (7)

Country Link
US (2) US5827649A (es)
EP (1) EP0379369B1 (es)
JP (1) JP3109810B2 (es)
AT (1) ATE142272T1 (es)
CA (1) CA2008096A1 (es)
DE (1) DE69028325T2 (es)
ES (1) ES2093633T3 (es)

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CA2046713A1 (en) * 1990-10-16 1992-04-17 Richard M. Martinelli Amplification of midivariant dna templates
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ES2112302T3 (es) 1991-10-11 1998-04-01 Behringwerke Ag Metodo para producir un polinucleotido para uso en amplificacion con un unico cebador y oligonucleotidos que contienen fosforotioato como cebadores para la amplificacion de acidos nucleicos.
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US7553619B2 (en) 2002-02-08 2009-06-30 Qiagen Gmbh Detection method using dissociated rolling circle amplification
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JP4728663B2 (ja) * 2004-03-22 2011-07-20 シスメックス株式会社 標的物質検出用プローブセット及び標的物質検出方法
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Also Published As

Publication number Publication date
EP0379369B1 (en) 1996-09-04
EP0379369A3 (en) 1991-07-03
US5827649A (en) 1998-10-27
JP3109810B2 (ja) 2000-11-20
EP0379369A2 (en) 1990-07-25
DE69028325D1 (de) 1996-10-10
DE69028325T2 (de) 1997-04-17
US6124090A (en) 2000-09-26
ATE142272T1 (de) 1996-09-15
JPH02268683A (ja) 1990-11-02
CA2008096A1 (en) 1990-07-19

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