ES2093640T3 - Produccion de hemoglobina y sus analogos mediante bacterias y levadura. - Google Patents

Produccion de hemoglobina y sus analogos mediante bacterias y levadura.

Info

Publication number
ES2093640T3
ES2093640T3 ES90610036T ES90610036T ES2093640T3 ES 2093640 T3 ES2093640 T3 ES 2093640T3 ES 90610036 T ES90610036 T ES 90610036T ES 90610036 T ES90610036 T ES 90610036T ES 2093640 T3 ES2093640 T3 ES 2093640T3
Authority
ES
Spain
Prior art keywords
alpha
beta
hemoglobin
globin
expressed
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
ES90610036T
Other languages
English (en)
Inventor
Stephen J Hoffman
Douglas L Looker
Mary S Rosendal
Gary L Stetler
Michael Wagenbach
Kiyoshi Nagai
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Medical Research Council
Somatogen Inc
Original Assignee
Medical Research Council
Somatogen Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Medical Research Council, Somatogen Inc filed Critical Medical Research Council
Application granted granted Critical
Publication of ES2093640T3 publication Critical patent/ES2093640T3/es
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K47/00—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient
    • A61K47/50—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates
    • A61K47/51—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates the non-active ingredient being a modifying agent
    • A61K47/62—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates the non-active ingredient being a modifying agent the modifying agent being a protein, peptide or polyamino acid
    • A61K47/64—Drug-peptide, drug-protein or drug-polyamino acid conjugates, i.e. the modifying agent being a peptide, protein or polyamino acid which is covalently bonded or complexed to a therapeutically active agent
    • A61K47/6445—Haemoglobin
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/795—Porphyrin- or corrin-ring-containing peptides
    • C07K14/805—Haemoglobins; Myoglobins
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/02—Fusion polypeptide containing a localisation/targetting motif containing a signal sequence
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/50—Fusion polypeptide containing protease site

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Plant Pathology (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Mycology (AREA)
  • Medicinal Chemistry (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Epidemiology (AREA)
  • Animal Behavior & Ethology (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Detergent Compositions (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Polysaccharides And Polysaccharide Derivatives (AREA)

Abstract

SUBUNIDADES DE ALFA-HEMOGLOBINA QUE ACTUAN COMO UN NUEVO POLIPEPTIDO DE DI-ALFA-GLOBINA RECOMBINANTE QUE CONSTA DE LAS DOS SUBUNIDADES ALFA CONECTADAS DIRECTAMENTE MEDIANTE UN ENLACE DE PEPTIDO O INDIRECTAMENTE MEDIANTE UN AMINOACIDO FLEXIBLE O AGENTE DE UNION DE PEPTIDOS. LA DI-ALFA-GLOBINA PUEDE COMBINARSE "IN VIVO" O "IN VITRO" CON BETA-GLOBINA Y HEMO PARA FORMAR HEMOGLOBINA. LA HEMOGLOBINA HUMANA TETRAMERICA Y LA DI-ALFA/BETA2 HEMOGLOBINA SE PRODUCEN EN S. CEREVISIAE MEDIANTE TRES TIPOS DE VECTORES DE EXPRESION: 1) DOS PLASMIDOS SEPARADOS QUE CONTIENEN RESPECTIVAMENTE GENES DE ALFA- Y BETA-GLOBINA EXPRESADOS EN CEPAS DIPLOIDES, 2) UN PLASMIDO QUE CONSTA DE GENES DE ALFA- Y BETA-GLOBINA EXPRESADOS EN CEPAS HAPLOIDES O DIPLOIDES, 3) UN PLASMIDO QUE CONSTA DE GENES DE DI-ALFA- Y BETA-GLOBINA EXPRESADOS EN CEPAS HAPLOIDES. LA FORMA TETRAMERICA O LAS SUBUNIDADES SEPARADAS PUEDEN RECUPERARSE DE LA FRACCION SOLUBLE. POR LO TANTO, PUEDEN PRODUCIRSE 3 TIPOS DE MOLECULAS DEL TIPO HEMOGLOBINA:DI-ALFA/BETA, DI-BETA/DOS ALFA O DI-ALFA/DI-BETA, CON UNA VIDA LARGA O MEDIA.
ES90610036T 1989-05-10 1990-05-10 Produccion de hemoglobina y sus analogos mediante bacterias y levadura. Expired - Lifetime ES2093640T3 (es)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US34962389A 1989-05-10 1989-05-10
US37416189A 1989-06-30 1989-06-30
US37911689A 1989-07-13 1989-07-13

Publications (1)

Publication Number Publication Date
ES2093640T3 true ES2093640T3 (es) 1997-01-01

Family

ID=27407902

Family Applications (2)

Application Number Title Priority Date Filing Date
ES95110064T Expired - Lifetime ES2204926T3 (es) 1989-05-10 1990-05-10 Procedimiento de preparacion de hemoglobina y derivados de hemoglobina a partir de manipulacion de bacterias y levaduras.
ES90610036T Expired - Lifetime ES2093640T3 (es) 1989-05-10 1990-05-10 Produccion de hemoglobina y sus analogos mediante bacterias y levadura.

Family Applications Before (1)

Application Number Title Priority Date Filing Date
ES95110064T Expired - Lifetime ES2204926T3 (es) 1989-05-10 1990-05-10 Procedimiento de preparacion de hemoglobina y derivados de hemoglobina a partir de manipulacion de bacterias y levaduras.

Country Status (18)

Country Link
EP (3) EP1380645A2 (es)
JP (1) JP3013902B2 (es)
KR (1) KR920701434A (es)
AT (2) ATE142692T1 (es)
AU (1) AU635744B2 (es)
CA (1) CA2050601C (es)
DE (2) DE69034091T2 (es)
DK (1) DK0402300T3 (es)
ES (2) ES2204926T3 (es)
FI (2) FI105104B (es)
GR (1) GR3021106T3 (es)
HU (1) HUT61591A (es)
IE (1) IE970086A1 (es)
IL (1) IL94361A (es)
NO (2) NO304027B1 (es)
NZ (1) NZ233642A (es)
SG (1) SG47987A1 (es)
WO (1) WO1990013645A1 (es)

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US6022849A (en) * 1987-05-16 2000-02-08 Baxter Biotech Technology Saarl Mutant recombinant hemoglobins containing heme pocket mutations
US6204009B1 (en) 1988-05-16 2001-03-20 BAXTER BIOTECH TECHNOLOGY SàRL Nucleic acids encoding mutant recombinant hemoglobins containing heme pocket mutations
US5844090A (en) * 1994-05-09 1998-12-01 Somatogen, Inc. Modified hemoglobin-like compounds
US5545727A (en) * 1989-05-10 1996-08-13 Somatogen, Inc. DNA encoding fused di-alpha globins and production of pseudotetrameric hemoglobin
US5599907A (en) * 1989-05-10 1997-02-04 Somatogen, Inc. Production and use of multimeric hemoglobins
US6150506A (en) * 1989-05-10 2000-11-21 Baxter Biotech Technology Sarl Modified hemoglobin-like compounds and methods of purifying same
WO1991013158A1 (en) * 1990-02-28 1991-09-05 Delta Biotechnology Limited Protein production in yeast
AU7744491A (en) * 1990-04-16 1991-11-11 Strohtech, Inc. Expression of recombinant hemoglobin in yeast
US6172039B1 (en) 1990-04-16 2001-01-09 Apex Bioscience, Inc. Expression of recombinant hemoglobin and hemoglobin variants in yeast
JPH06504056A (ja) * 1990-12-20 1994-05-12 ザ・ユニバーシティ・オブ・アラバマ・リサーチ・ファウンデーション トランスジェニック架橋ヘモグロビン
US5610137A (en) * 1990-12-20 1997-03-11 The Uab Research Foundation Transgenic, cross-linked hemoglobin
US5714345A (en) * 1990-12-24 1998-02-03 Pharmaceutical Proteins Limited Increased expression of a gene by a second transferred mammary gland specific sequence transgenic
GB9028062D0 (en) * 1990-12-24 1991-02-13 Agricultural & Food Res Production of transgenic animals
WO1993008831A1 (en) * 1991-10-30 1993-05-13 Strohtech, Inc. Expression of recombinant hemoglobin and hemoglobin variants in yeast
EP0611306B1 (en) * 1991-11-08 1998-07-08 Somatogen, Inc. Hemoglobins as drug delivery agents
FR2688784B1 (fr) * 1992-03-18 1995-06-30 Pasteur Merieux Serums Vacc Transporteur d'oxygene.
US6610654B2 (en) 1993-03-31 2003-08-26 Wellstat Therapeutics Corporation Inhibitor of stem cell proliferation and uses thereof
EP0691988B1 (en) 1993-03-31 2002-10-02 Pro-Neuron, Inc. Inhibitor of stem cell proliferation and uses thereof
US5939391A (en) * 1993-03-31 1999-08-17 Pro-Neuron, Inc. Hemoglobin alpha chain peptide fragments useful for inhibiting stem cell proliferation
US5554638A (en) * 1993-05-24 1996-09-10 Duke University Methods for improving therapeutic effectiveness of agents for the treatment of solid tumors and other disorders
US5612310A (en) * 1993-05-24 1997-03-18 Duke University Methods for improving therapeutic effectiveness of agents for the treatment of solid tumors and other disorders
US5840851A (en) * 1993-07-23 1998-11-24 Plomer; J. Jeffrey Purification of hemoglobin
US5665869A (en) * 1993-11-15 1997-09-09 Somatogen, Inc. Method for the rapid removal of protoporphyrin from protoporphyrin IX-containing solutions of hemoglobin
US6140071A (en) * 1994-01-27 2000-10-31 Somatogen, Inc. Proteins with mutations to decrease N-terminal methylation
US6242417B1 (en) 1994-03-08 2001-06-05 Somatogen, Inc. Stabilized compositions containing hemoglobin
US5631219A (en) * 1994-03-08 1997-05-20 Somatogen, Inc. Method of stimulating hematopoiesis with hemoglobin
WO1997004110A1 (en) * 1995-07-14 1997-02-06 Somatogen, Inc. Methods for increasing protein expression
US5824511A (en) * 1995-08-01 1998-10-20 University Technology Corporation Method for enhancing the production of hemoproteins
CA2231245A1 (en) * 1995-10-23 1997-05-01 Mark S. Hargrove Hemoglobin mutants that reduce heme loss
US6812207B1 (en) 1995-10-23 2004-11-02 William Marsh Rice University Hemoglobin mutants that reduce heme loss
CA2236794A1 (en) * 1995-11-30 1997-06-05 Bruce A. Kerwin Method for control of functionality during cross-linking of hemoglobins
WO1997023631A2 (en) * 1995-12-22 1997-07-03 Somatogen, Inc. Globins containing binding domains
EP0868521A2 (en) 1995-12-22 1998-10-07 Somatogen Inc. Globins containing binding domains
US5861483A (en) * 1996-04-03 1999-01-19 Pro-Neuron, Inc. Inhibitor of stem cell proliferation and uses thereof
EP0954586A1 (en) * 1996-10-18 1999-11-10 Baxter Biotech Technology S.A.R.L. Methods of reducing the levels of protoporphyrin ix in recombinant hemoglobin preparations
EP1950298A3 (en) 1997-05-02 2008-12-24 Baxter Biotech Technology S.A.R.L. Hemoglobin mutants with increased soluble expression and/or reduced nitric oxide scavenging
DE10036491A1 (de) 2000-07-25 2002-02-07 Roche Diagnostics Gmbh Expression von Alkalischer Phosphatase in Hefe
DK1368483T3 (da) 2001-03-09 2012-11-05 Genentech Inc Fremgangsmåde til fremstilling af polypeptider
US7494974B2 (en) 2006-10-24 2009-02-24 Ikor, Inc. Carboxymethylated cross-linked tetrameric hemoglobin
US7504377B2 (en) 2006-10-23 2009-03-17 Ikor, Inc. Nitric oxide-blocked cross-linked tetrameric hemoglobin
EP2178551A4 (en) * 2007-07-24 2010-07-21 Sanare Invest As NOVEL POLYPEPTIDES WITH ANTITUMOR EFFECT
EP2058398A1 (en) * 2007-11-09 2009-05-13 Nipro Corporation Production of recombinant human hemoglobin using pichia yeast
US10752672B1 (en) * 2019-02-01 2020-08-25 Cheer Global Limited Recombinant hemoglobins and methods of preparation and use thereof
CN114929736A (zh) * 2020-01-10 2022-08-19 尹特荣生物科技株式会社 一种利用大肠杆菌制备猪肌红蛋白的方法
EP4090677A4 (en) * 2020-01-10 2024-01-10 Intron Biotechnology, Inc. METHOD FOR PRODUCING BOvine MYOGLOBIN USING ESCHERICHIA COLI
CN116203144B (zh) * 2022-05-11 2023-10-27 重庆医科大学附属儿童医院 测定血红蛋白中各型珠蛋白链比率的方法及其应用
EP4628511A4 (en) * 2022-11-30 2026-04-15 Kangma Healthcode Shanghai Biotech Co Ltd Recombinant hemoglobin
CN121046493A (zh) * 2024-05-30 2025-12-02 康码（上海）生物科技有限公司 一种无细胞合成血红素蛋白或肽链的方法

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Also Published As

Publication number Publication date
DE69028448D1 (de) 1996-10-17
WO1990013645A1 (en) 1990-11-15
DE69034091T2 (de) 2004-04-22
NO973848D0 (no) 1997-08-21
ES2204926T3 (es) 2004-05-01
EP0402300B1 (en) 1996-09-11
SG47987A1 (en) 1998-04-17
NO914387L (no) 1991-11-08
FI915279A0 (fi) 1991-11-08
ATE246246T1 (de) 2003-08-15
EP0700997A1 (en) 1996-03-13
EP0402300A2 (en) 1990-12-12
EP1380645A2 (en) 2004-01-14
EP0402300A3 (en) 1991-01-30
HU905111D0 (en) 1992-02-28
IL94361A (en) 1995-07-31
NO315568B1 (no) 2003-09-22
JPH05503627A (ja) 1993-06-17
CA2050601C (en) 2000-09-19
DE69028448T2 (de) 1997-02-06
KR920701434A (ko) 1992-08-11
GR3021106T3 (en) 1996-12-31
FI105104B (fi) 2000-06-15
FI20000025L (fi) 2000-01-05
DE69034091D1 (de) 2003-09-04
IL94361A0 (en) 1991-03-10
JP3013902B2 (ja) 2000-02-28
NO914387D0 (no) 1991-11-08
AU635744B2 (en) 1993-04-01
NO973848L (no) 1997-08-21
HUT61591A (en) 1993-01-28
FI107938B (fi) 2001-10-31
EP0700997B1 (en) 2003-07-30
IE970086A1 (en) 2000-02-23
ATE142692T1 (de) 1996-09-15
CA2050601A1 (en) 1990-11-11
NO304027B1 (no) 1998-10-12
AU5675690A (en) 1990-11-29
NZ233642A (en) 1992-12-23
DK0402300T3 (da) 1996-09-30

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