ES2093939T3 - Metodo para preparar placa de gel acuoso de poliacrilamida para electroforesis. - Google Patents

Metodo para preparar placa de gel acuoso de poliacrilamida para electroforesis.

Info

Publication number
ES2093939T3
ES2093939T3 ES93400260T ES93400260T ES2093939T3 ES 2093939 T3 ES2093939 T3 ES 2093939T3 ES 93400260 T ES93400260 T ES 93400260T ES 93400260 T ES93400260 T ES 93400260T ES 2093939 T3 ES2093939 T3 ES 2093939T3
Authority
ES
Spain
Prior art keywords
electrophoresis
gel
container
gel plate
polyacrylamide gel
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
ES93400260T
Other languages
English (en)
Inventor
Nobuyoshi Ebata
Kazuaki Notsu
Akiko Udagawa
Mieko Shiratori
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Sekisui Medical Co Ltd
Original Assignee
Daiichi Pure Chemicals Co Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Daiichi Pure Chemicals Co Ltd filed Critical Daiichi Pure Chemicals Co Ltd
Application granted granted Critical
Publication of ES2093939T3 publication Critical patent/ES2093939T3/es
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/416—Systems
    • G01N27/447—Systems using electrophoresis
    • G01N27/44704—Details; Accessories

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Molecular Biology (AREA)
  • Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Electrochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Addition Polymer Or Copolymer, Post-Treatments, Or Chemical Modifications (AREA)
  • Peptides Or Proteins (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

SE DESCRIBE UN METODO PARA LA PREPARACION POR LOTES DE PLACA DE GELES ACUOSOS DE POLIACRILAMIDA PARA ELECTROFORESIS. EL METODO COMPRENDE LA COLOCACION DE LOS DIFERENTES SOPORTES DE LAS PLACAS DE GEL (6) UNOS AL LADO DE OTROS EN UN CONTENEDOR (1) DONDE SE FORMA EL GEL ESTANCO AL AIRE, LA ELIMINACION DEL OXIGENO DEL CONTENEDOR O LA INTRODUCCION DE UN GAS EXENTO DE OXIGENO EN DICHO CONTENEDOR, LA CARGA DE UNA SOLUCION FORMADORA DE GEL DENTRO DE DICHO CONTENEDOR, Y LA GELATINIZACION DE LA SOLUCION. SE PUEDE PRODUCIR FACILMENTE UNA GRAN CANTIDAD DE PLACAS DE GEL DE POLIACRILAMIDA DE ELEVADA CALIDAD PARA ELECTROFORESIS CON UNA CALIDAD HOMOGENEA Y UN ELEVADO PODER DE RESOLUCION, EN UN METODO POR LOTES AUTOMATIZADO EN UN CORTO PERIODO DE TIEMPO.
ES93400260T 1992-02-07 1993-02-03 Metodo para preparar placa de gel acuoso de poliacrilamida para electroforesis. Expired - Lifetime ES2093939T3 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
JP4022705A JP3071927B2 (ja) 1992-02-07 1992-02-07 電気泳動用ゲルプレートの製造方法

Publications (1)

Publication Number Publication Date
ES2093939T3 true ES2093939T3 (es) 1997-01-01

Family

ID=12090276

Family Applications (1)

Application Number Title Priority Date Filing Date
ES93400260T Expired - Lifetime ES2093939T3 (es) 1992-02-07 1993-02-03 Metodo para preparar placa de gel acuoso de poliacrilamida para electroforesis.

Country Status (9)

Country Link
US (1) US5350552A (es)
EP (1) EP0555143B1 (es)
JP (1) JP3071927B2 (es)
AT (1) ATE143500T1 (es)
CA (1) CA2088548C (es)
DE (1) DE69304941T2 (es)
DK (1) DK0555143T3 (es)
ES (1) ES2093939T3 (es)
GR (1) GR3021961T3 (es)

Families Citing this family (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5433837A (en) * 1993-08-27 1995-07-18 E. I. Du Pont De Nemours And Company Gel cassette for enhanced electrophoretic separation and processes for the preparation thereof
EP0684468A3 (en) * 1994-05-27 1997-03-26 Eastman Kodak Co Condensation-free electrophoresis device.
US5507934A (en) * 1994-11-01 1996-04-16 Visible Genetics Inc. Apparatus for preparing gels for use in electrophoretic separations and similar applications
US6110344A (en) * 1994-11-01 2000-08-29 Visible Genetics, Inc. Gel-forming insert for electrophoresis gels
US5520790A (en) * 1995-04-11 1996-05-28 Life Technologies, Inc. Gel casting system
US5827418A (en) * 1996-10-11 1998-10-27 Hoefer Pharmacia Biotech, Inc. Electrophoresis cassette
US6136172A (en) * 1997-06-30 2000-10-24 Visible Genetics Inc. Gel-forming insert for electrophoresis gels
AUPP290298A0 (en) * 1998-04-09 1998-05-07 Life Therapeutics Limited Improved electrophoresis gel and gel-forming apparatus
AU740130B2 (en) * 1998-04-09 2001-11-01 Life Therapeutics Limited Electrophoresis gel and gel-forming apparatus
US6187250B1 (en) * 1998-08-19 2001-02-13 James Champagne Continuous gel casting method and apparatus
JP4587580B2 (ja) * 2001-02-26 2010-11-24 独立行政法人科学技術振興機構 電気泳動用強化ゲルとその製造方法
US20050249882A1 (en) * 2004-05-04 2005-11-10 Shaorong Liu Coatings for molecule transport and separations
JP2009156767A (ja) * 2007-12-27 2009-07-16 National Institute Of Advanced Industrial & Technology タンパク質アレイ基板
EP4039285A1 (en) * 2021-02-04 2022-08-10 ETH Zurich Controlling the tribological properties of gels

Family Cites Families (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3551541A (en) * 1969-12-11 1970-12-29 Carlo Rossetti Method of polymerizing monomeric material into cast polymer sheets and the apparatus for the casting of said polymeric sheets
US4169036A (en) * 1978-03-24 1979-09-25 The United States Of America As Represented By The United States Department Of Energy System for loading slab-gel holders for electrophoresis separation
US4416761A (en) * 1982-07-27 1983-11-22 The United States Of America As Represented By The Department Of Health And Human Services Multi slab gel casting electrophoresis apparatus
US4810456A (en) * 1986-12-24 1989-03-07 Hewlett-Packard Company Method of preventing shrinkage defects in electrophoretic gel columns
US4909977A (en) * 1987-05-20 1990-03-20 Bios Corporation Method for molding thin gel slabs horizontally with integrally molded large volume sample wells
JPH01165949A (ja) * 1987-12-22 1989-06-29 Hideyuki Nishizawa 電気泳動用繊維強化ゲルの作成法
JPH01165948A (ja) * 1987-12-22 1989-06-29 Hideyuki Nishizawa 電気泳動用繊維強化ゲルの作成装置
EP0334615B1 (en) * 1988-03-22 1994-11-17 Erik Nieman Electrophoresis apparatus
JPH0477868A (ja) * 1990-07-14 1992-03-11 Tsutomu Sato 画像情報の整理方法
DE9110289U1 (de) * 1990-08-29 1991-12-19 Michov, Budin, Dr. Dr., 8050 Freising Homogene Elektrophoreseplatte zur Durchführung einer Proteintrennung ....
US5069773A (en) * 1991-02-06 1991-12-03 Frangioni John V Electrophoretic gel forming system
US5188790A (en) * 1991-04-30 1993-02-23 Owl Scientific Plastics, Inc. Casting method for forming a gel matrix

Also Published As

Publication number Publication date
JPH05223779A (ja) 1993-08-31
US5350552A (en) 1994-09-27
DE69304941T2 (de) 1997-02-20
DE69304941D1 (de) 1996-10-31
EP0555143A1 (en) 1993-08-11
GR3021961T3 (en) 1997-03-31
EP0555143B1 (en) 1996-09-25
CA2088548A1 (en) 1993-08-08
DK0555143T3 (da) 1996-12-23
JP3071927B2 (ja) 2000-07-31
CA2088548C (en) 2003-05-13
ATE143500T1 (de) 1996-10-15

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