ES2096584T3 - Polipeptidos y proteinas variantes que poseen afinidad potenciada por matrices de afinidad de metal inmovilizado. - Google Patents

Polipeptidos y proteinas variantes que poseen afinidad potenciada por matrices de afinidad de metal inmovilizado.

Info

Publication number
ES2096584T3
ES2096584T3 ES90870109T ES90870109T ES2096584T3 ES 2096584 T3 ES2096584 T3 ES 2096584T3 ES 90870109 T ES90870109 T ES 90870109T ES 90870109 T ES90870109 T ES 90870109T ES 2096584 T3 ES2096584 T3 ES 2096584T3
Authority
ES
Spain
Prior art keywords
affinity
proteins
enhanced
variant polypeptides
immobilized metal
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
ES90870109T
Other languages
English (en)
Inventor
Barry Lant Haymore
Gary Stephen Bild
Gwen Grabowski Krivi
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Monsanto Co
Original Assignee
Monsanto Co
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Monsanto Co filed Critical Monsanto Co
Application granted granted Critical
Publication of ES2096584T3 publication Critical patent/ES2096584T3/es
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/575—Hormones
    • C07K14/61—Growth hormone [GH], i.e. somatotropin
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/107—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/575—Hormones
    • C07K14/65—Insulin-like growth factors, i.e. somatomedins, e.g. IGF-1, IGF-2

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Molecular Biology (AREA)
  • Endocrinology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Medicinal Chemistry (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Zoology (AREA)
  • Toxicology (AREA)
  • Diabetes (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Peptides Or Proteins (AREA)
  • Feed For Specific Animals (AREA)
  • Fodder In General (AREA)

Abstract

PROTEINAS Y POLIPEPTIDOS VARIANTES CON UNA MEJOR AFINIDAD, ES DECIR, MAYOR FUERZA COHESIVA, PARA RESINAS DE METAL DE AFINIDAD INMOVILIZADA, CON UN RESIDUO DE ACIDO ASPARTICO O HISTIDINA EN UNA O MAS POSICIONES QUE, EN UNA PROTEINA NATURAL ESTAN OCUPADAS POR OTROS RESIDUOS AMINOACIDOS, DE FORMA QUE LA PROTEINA VARIANTE POSEE UNA SECUENCIA DE AMINOACIDO METALICA. LA SECUENCIA ESPECIFICA DEPENDE DE LA ESTRUCTURA SECUNDARIA ASOCIADA A LA PORCION DE LA PROTEINA O POLIPEPTIDO QUE INCLUYA DICHA SECUENCIA.
ES90870109T 1989-07-21 1990-07-16 Polipeptidos y proteinas variantes que poseen afinidad potenciada por matrices de afinidad de metal inmovilizado. Expired - Lifetime ES2096584T3 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US07/383,778 US5115102A (en) 1989-07-21 1989-07-21 Variant proteins and polypeptides possessing enhanced affinity for immobilized-metal affinity matrices

Publications (1)

Publication Number Publication Date
ES2096584T3 true ES2096584T3 (es) 1997-03-16

Family

ID=23514691

Family Applications (1)

Application Number Title Priority Date Filing Date
ES90870109T Expired - Lifetime ES2096584T3 (es) 1989-07-21 1990-07-16 Polipeptidos y proteinas variantes que poseen afinidad potenciada por matrices de afinidad de metal inmovilizado.

Country Status (9)

Country Link
US (1) US5115102A (es)
EP (1) EP0409814B1 (es)
JP (1) JP3576161B2 (es)
AT (1) ATE147433T1 (es)
CA (1) CA2021722C (es)
DE (1) DE69029615T2 (es)
DK (1) DK0409814T3 (es)
ES (1) ES2096584T3 (es)
GR (1) GR3023052T3 (es)

Families Citing this family (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5821088A (en) * 1990-05-11 1998-10-13 Siga Pharmaceuticals, Inc. Use of gram-positive bacteria to express recombinant proteins
WO1992008788A1 (en) * 1990-11-19 1992-05-29 The Board Of Trustees Of The University Of Illinois Mutant orientable proteins and coated substrates
US5646016A (en) * 1991-02-06 1997-07-08 Genetics Institute, Inc. Peptide and protein fusions to thioredoxin, thioredoxin-like molecules, and modified thioredoxin-like molecules
DE4105480A1 (de) * 1991-02-21 1992-08-27 Boehringer Mannheim Gmbh Verbesserte aktivierung von rekombinanten proteinen
JPH10504971A (ja) * 1994-09-08 1998-05-19 カイロン コーポレイション インスリン様増殖因子の改善された生産方法
US6068993A (en) * 1997-07-02 2000-05-30 Biobras Sa Vector for expression of heterologous protein and methods for extracting recombinant protein and for purifying isolated recombinant insulin
US5932102A (en) * 1998-01-12 1999-08-03 Schering Corporation Immobilized metal, affinity chromatography
DE19822823A1 (de) * 1998-05-20 2000-02-10 Basf Ag Neue Peptidfragmente zur Reinigung von Proteinen
US20100233678A1 (en) * 2007-11-13 2010-09-16 Beadling Leslie C Tunable affinity ligands for the separation and detection of target substances

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4569794A (en) * 1984-12-05 1986-02-11 Eli Lilly And Company Process for purifying proteins and compounds useful in such process
WO1988008028A1 (en) * 1987-04-06 1988-10-20 Genex Corporation The engineering of electrostatic interactions at metal ion binding sites for the stabilization of proteins

Also Published As

Publication number Publication date
JP3576161B2 (ja) 2004-10-13
GR3023052T3 (en) 1997-07-30
EP0409814B1 (en) 1997-01-08
US5115102A (en) 1992-05-19
CA2021722A1 (en) 1991-01-22
DK0409814T3 (da) 1997-06-30
EP0409814A1 (en) 1991-01-23
DE69029615T2 (de) 1997-06-12
ATE147433T1 (de) 1997-01-15
JPH03101693A (ja) 1991-04-26
CA2021722C (en) 2002-12-17
DE69029615D1 (de) 1997-02-20

Similar Documents

Publication Publication Date Title
DK0397633T3 (da) Fibronectinbindende protein samt fremstilling deraf
DE69433301D1 (de) Methoden zur erhöhung der biologischen aktivität von chemokinen
NZ503850A (en) April - a novel protein with growth effects
DK0758383T3 (da) Oplöselige polypeptidfraktioner af LAG-3-proteinet; fremgangsmåde til fremstilling; terapeutisk sammensætning; antiidiotypisk antistof
PT1121382E (pt) Proteinas de fusao do interferao beta e as respectivas utilizacoes
DE3587814D1 (de) Verfahren zum erhalten von dns, rns, peptiden, polypeptiden oder proteinen durch dns-rekombinant-verfahren.
IL138608A0 (en) Antibody variants and fragments thereof
FI864427A0 (fi) Menetelmä polypeptidin valmistamiseksi, joka on komplementaarinen ainakin osalle alkuperäistä peptidiä tai proteiinia, ja komplementaarisen polypeptidin käyttö
DE68917323D1 (de) Expressions-Vektor für Fusions-Proteine und Immunogen-Proteine.
IL134011A0 (en) Adipocyte-specific protein homologs
ATE143375T1 (de) Methode zur quantitativen bestimmung von thyrotropin (tsh) oder anti-thryrotropin-rezeptor autoantikörper (anti-tshr) unter verwendung eines rekombinanten thyrotropin rezeptor polypeptides und kit zur durchführung dieser methode
DK0409814T3 (da) Variantproteiner og -polypeptider, der har forøget affinitet over for immobiliseret-metalaffinitetsmatricer
PT641861E (pt) Processo de producao de polipeptidos proteinas ou peptidos hidrofobicos
NO882380L (no) Fibfonektin-bindende protein, samt fremstilling derav.
DK0863155T3 (da) Suppression af T-celle-proliferation under anvendelse af peptidfragmenter af myelin basisk protein
PT728208E (pt) Endo-transglicosilase para o xiloglucano do tomate
NO922421D0 (no) Collagenbindende protein, samt fremstilling derav
EP0309566A4 (en) Stlv-iii-related polypeptides, diagnostic systems and assay methods.
ES2074591T3 (es) Expresion de polipeptidos de vih 1 y 2 y su utilizacion.
DE69015372D1 (de) Protein-testverfahren.
NO900321D0 (no) Herdbar blanding som omfatter en aminoharpikskomponent.
DK1342729T3 (da) Antistoffer og antisense-molekyler for G-koblet receptor der udviser selektiv affinitet for ATP
DK450187D0 (da) Fremgangsmaade til fremstilling af proteiner
JPS6467197A (en) Enzymatically inactive and immunologically active b-galatosidase-mutagenic protein, its preparation, immulogical assay of serum protein, and enzyme-immunoassay reagent
NO900323D0 (no) Herdbar blanding som omfatter en aminoharpikskomponent.

Legal Events

Date Code Title Description
FG2A Definitive protection

Ref document number: 409814

Country of ref document: ES