ES2096671T3 - Procedimiento para la preparacion de glutarilacilasa en grandes cantidades. - Google Patents

Procedimiento para la preparacion de glutarilacilasa en grandes cantidades.

Info

Publication number
ES2096671T3
ES2096671T3 ES92104583T ES92104583T ES2096671T3 ES 2096671 T3 ES2096671 T3 ES 2096671T3 ES 92104583 T ES92104583 T ES 92104583T ES 92104583 T ES92104583 T ES 92104583T ES 2096671 T3 ES2096671 T3 ES 2096671T3
Authority
ES
Spain
Prior art keywords
glutarilacilase
procedure
preparation
large quantities
dsm
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
ES92104583T
Other languages
English (en)
Inventor
Klaus-Peter Dr Koller
Gunther Johannes Dr Riess
Werner Dr Aretz
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Hoechst AG
Original Assignee
Hoechst AG
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Hoechst AG filed Critical Hoechst AG
Application granted granted Critical
Publication of ES2096671T3 publication Critical patent/ES2096671T3/es
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/78—Hydrolases (3) acting on carbon to nitrogen bonds other than peptide bonds (3.5)
    • C12N9/80—Hydrolases (3) acting on carbon to nitrogen bonds other than peptide bonds (3.5) acting on amide bonds in linear amides (3.5.1)

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Biomedical Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Saccharide Compounds (AREA)

Abstract

EL GEN CODIFICANTE DE LA GLUTARILACILASA (GA) CONTENIDA EN EL PLASMIDO PCM145 (DSM 6409) PERMITE LA EXPRESION DE GA EN E.COLI EN GRANDES RENDIMIENTOS.
ES92104583T 1991-03-18 1992-03-17 Procedimiento para la preparacion de glutarilacilasa en grandes cantidades. Expired - Lifetime ES2096671T3 (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE4108823 1991-03-18
DE4136389A DE4136389A1 (de) 1991-03-18 1991-11-05 Verfahren zur herstellung von glutarylacylase in grossen mengen

Publications (1)

Publication Number Publication Date
ES2096671T3 true ES2096671T3 (es) 1997-03-16

Family

ID=25902010

Family Applications (1)

Application Number Title Priority Date Filing Date
ES92104583T Expired - Lifetime ES2096671T3 (es) 1991-03-18 1992-03-17 Procedimiento para la preparacion de glutarilacilasa en grandes cantidades.

Country Status (15)

Country Link
EP (1) EP0504798B1 (es)
JP (1) JP3366664B2 (es)
KR (1) KR100211002B1 (es)
CN (1) CN1065292A (es)
AT (1) ATE145238T1 (es)
CA (1) CA2063176C (es)
CY (1) CY2115B1 (es)
CZ (1) CZ284405B6 (es)
DE (2) DE4136389A1 (es)
DK (1) DK0504798T3 (es)
ES (1) ES2096671T3 (es)
GR (1) GR3021610T3 (es)
HK (1) HK1006853A1 (es)
IL (1) IL101258A0 (es)
SK (1) SK280242B6 (es)

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE4337787A1 (de) * 1993-11-05 1995-05-11 Hoechst Ag Sauerstoffabhängige Fermentation von Mikroorganismen
IT1269180B (it) * 1994-01-14 1997-03-21 Mini Ricerca Scient Tecnolog Processo per la produzione dell'enzima acido 7beta- (4-carbossibutanamido)-cefalosporanico acilasi
DE4437420A1 (de) * 1994-10-19 1996-04-25 Hoechst Ag Verbessertes, induktionsfreies Verfahren zur gentechnischen Herstellung von Glutarylamidase
US5766871A (en) * 1996-11-27 1998-06-16 Food Industry Research And Development Institute Screening and characterization of glutaryl-7-aminocephalosporanic acid acylase
DE19915938A1 (de) 1999-04-09 2000-10-19 Aventis Pharma Gmbh Herstellung von pankreatischer Procarboxypeptidase B, Isoformen und Muteinen davon und ihre Verwendung
DE19924632B4 (de) * 1999-05-28 2006-04-13 Sanofi-Aventis Deutschland Gmbh Verfahren zur Verzögerung der Desaktivierung von Glutarylamidase während einer Enzymkatalyse

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
HU194307B (en) * 1983-09-16 1988-01-28 Richter Gedeon Vegyeszet Process for producing plasmidvectors of high copy number
DE3926076A1 (de) * 1989-04-21 1990-10-25 Boehringer Mannheim Gmbh Rekombinante dna und expressionsvektor

Also Published As

Publication number Publication date
JP3366664B2 (ja) 2003-01-14
SK280242B6 (sk) 1999-10-08
KR920018222A (ko) 1992-10-21
EP0504798B1 (de) 1996-11-13
CS80492A3 (en) 1992-10-14
DE59207509D1 (de) 1996-12-19
ATE145238T1 (de) 1996-11-15
CY2115B1 (en) 2002-04-26
GR3021610T3 (en) 1997-02-28
DE4136389A1 (de) 1992-10-08
DE4136389C2 (es) 1993-07-01
JPH05207879A (ja) 1993-08-20
CZ284405B6 (cs) 1998-11-11
CN1065292A (zh) 1992-10-14
KR100211002B1 (ko) 1999-07-15
DK0504798T3 (da) 1997-04-07
IL101258A0 (en) 1992-11-15
EP0504798A1 (de) 1992-09-23
HK1006853A1 (en) 1999-03-19
CA2063176A1 (en) 1992-09-19
CA2063176C (en) 2004-06-01

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