ES2099249T3 - Gel binario mejorado de galactomanano-agarosa para electroforesis de acidos nucleicos. - Google Patents

Gel binario mejorado de galactomanano-agarosa para electroforesis de acidos nucleicos.

Info

Publication number
ES2099249T3
ES2099249T3 ES92905091T ES92905091T ES2099249T3 ES 2099249 T3 ES2099249 T3 ES 2099249T3 ES 92905091 T ES92905091 T ES 92905091T ES 92905091 T ES92905091 T ES 92905091T ES 2099249 T3 ES2099249 T3 ES 2099249T3
Authority
ES
Spain
Prior art keywords
gel
nucleic acids
galactomanano
agarosa
electrophoresis
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
ES92905091T
Other languages
English (en)
Inventor
Daniel Perlman
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Brandeis University
Original Assignee
Brandeis University
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Brandeis University filed Critical Brandeis University
Application granted granted Critical
Publication of ES2099249T3 publication Critical patent/ES2099249T3/es
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/416—Systems
    • G01N27/447—Systems using electrophoresis
    • G01N27/44704—Details; Accessories
    • G01N27/44747—Composition of gel or of carrier mixture
    • C—CHEMISTRY; METALLURGY
    • C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
    • C08L—COMPOSITIONS OF MACROMOLECULAR COMPOUNDS
    • C08L5/00—Compositions of polysaccharides or of their derivatives not provided for in groups C08L1/00 or C08L3/00
    • C08L5/12—Agar or agar-agar, i.e. mixture of agarose and agaropectin; Derivatives thereof
    • C—CHEMISTRY; METALLURGY
    • C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
    • C08L—COMPOSITIONS OF MACROMOLECULAR COMPOUNDS
    • C08L5/00—Compositions of polysaccharides or of their derivatives not provided for in groups C08L1/00 or C08L3/00
    • C08L5/14—Hemicellulose; Derivatives thereof
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S522/00—Synthetic resins or natural rubbers -- part of the class 520 series
    • Y10S522/911—Specified treatment involving megarad or less

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Molecular Biology (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Analytical Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Engineering & Computer Science (AREA)
  • Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Biochemistry (AREA)
  • Polymers & Plastics (AREA)
  • Medicinal Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Electrochemistry (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biophysics (AREA)
  • Genetics & Genomics (AREA)
  • Dispersion Chemistry (AREA)
  • Microbiology (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Compositions Of Macromolecular Compounds (AREA)
  • Polysaccharides And Polysaccharide Derivatives (AREA)
  • Saccharide Compounds (AREA)
  • Colloid Chemistry (AREA)
  • Processes Of Treating Macromolecular Substances (AREA)

Abstract

UN GEL ELECTROFORETICO DE AGAROSA, UN METODO PARA LA FORMACION DEL GEL Y UN GALACTOMANAN CLARIFICADO, IRRADIADO CON RAYOS GAMMA, INTERMEDIO, SOLIDO (GAMMACGM) PARA UTILIZARLO CON EL GEL. EL GEL SE FORMA MEDIANTE LA ADICION DE GAMMA-CGM PARA CLARIFICAR OPTICAMENTE EL GEL Y PODER UTILIZARLO EN UNA RESOLUCION Y FRACCIONAMIENTO ELECTROFORETICO DE OLIGONUCLEOTIDOS DE DIFERENTE PESO MOLECULAR. EL GEL INCLUYE AGAROSA, UN TAMPON ELECTROFORETICO PARA ACIDOS NUCLEICOS Y UN CAUCHO VEGETAL CGM SOMETIDO A ENTRE 0,1 Y 4,0 MEGARADS DE RADIACION GAMMA IONIZANTE.
ES92905091T 1991-01-24 1992-01-24 Gel binario mejorado de galactomanano-agarosa para electroforesis de acidos nucleicos. Expired - Lifetime ES2099249T3 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US07/645,203 US5230832A (en) 1991-01-24 1991-01-24 Galactomannan-agarose binary gel for nucleic acid electrophoresis

Publications (1)

Publication Number Publication Date
ES2099249T3 true ES2099249T3 (es) 1997-05-16

Family

ID=24588054

Family Applications (1)

Application Number Title Priority Date Filing Date
ES92905091T Expired - Lifetime ES2099249T3 (es) 1991-01-24 1992-01-24 Gel binario mejorado de galactomanano-agarosa para electroforesis de acidos nucleicos.

Country Status (8)

Country Link
US (1) US5230832A (es)
EP (1) EP0569494B1 (es)
JP (1) JPH0786503B2 (es)
AT (1) ATE150664T1 (es)
DE (1) DE69218586T2 (es)
DK (1) DK0569494T3 (es)
ES (1) ES2099249T3 (es)
WO (1) WO1992012789A1 (es)

Families Citing this family (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5885432A (en) * 1992-11-05 1999-03-23 Soane Biosciences Un-crosslinked polymeric media for electrophoresis
DE19920688A1 (de) * 1999-05-05 2000-11-23 Manfred Kuhn Radiolytisch modifizierte Heteropolysaccharide
US6586590B1 (en) * 2000-07-03 2003-07-01 Marine Bioproducts International Clarified hydrocolloids of undiminished properties and method of producing same
US7408057B2 (en) * 2000-07-03 2008-08-05 Marine Bioproducts Intenational Clarified hydrocolloids of undiminished properties and method of producing same
GB0316742D0 (en) * 2003-07-17 2003-08-20 Fermentas Uab Electrophoretic gels and their manufacture
JP7072830B2 (ja) * 2017-07-26 2022-05-23 国立大学法人広島大学 高分離能を有する電気泳動用ゲルの製造方法およびその製造キット
JP6966071B2 (ja) * 2017-11-29 2021-11-10 国立大学法人広島大学 ゲル分離能向上剤の製造方法およびゲル分離能向上剤の利用
US11691122B2 (en) 2018-11-02 2023-07-04 Advanced Aesthetic Technologies, Inc. Irradiated agarose, compositions thereof, and related methods
CN112996551B (zh) * 2018-11-02 2023-08-29 先进美学科技公司 辐照琼脂糖、其组合物及相关方法

Family Cites Families (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3034974A (en) * 1958-01-28 1962-05-15 Gen Foods Corp Polysaccharide extraction by irradiation
FR2357188A2 (fr) * 1976-07-09 1978-02-03 Ceca Sa Perfectionnements aux compositions gelifiantes pour produits alimentaires
US4290911A (en) * 1979-02-07 1981-09-22 Fmc Corporation Agarose composition, aqueous gel and method of making same
US4321121A (en) * 1981-01-19 1982-03-23 Beckman Instruments, Inc. Electrophoretic technique for separating lactate dehydrogenase isoenzymes and improved electrophoretic gel for use therein
US4704198A (en) * 1984-04-27 1987-11-03 E. I. Du Pont De Nemours And Company Porosity gradient electrophoresis gel
IT1213384B (it) * 1986-11-24 1989-12-20 Lab Derivati Organici Mediolan Processo per la preparazione controllata di gilcosaminoglicani a basso peso molecolare.
DK0454763T3 (da) * 1989-01-13 1996-11-25 Fmc Corp Adskillelsesgeler og gelsystemer på polysaccharidbasis til stabelelektroforese og fremgangsmåde til støbning af en elektroforesegel
US4983268A (en) * 1989-08-03 1991-01-08 Fmc Corporation High gel strength low electroendosmosis agarose
EP0455841A1 (en) * 1990-05-05 1991-11-13 Studiengesellschaft Kohle mbH Method for the production of gel filled capillaries for capillary gel electrophoresis

Also Published As

Publication number Publication date
WO1992012789A1 (en) 1992-08-06
DE69218586D1 (de) 1997-04-30
JPH0786503B2 (ja) 1995-09-20
US5230832A (en) 1993-07-27
DE69218586T2 (de) 1997-07-03
EP0569494A1 (en) 1993-11-18
ATE150664T1 (de) 1997-04-15
EP0569494A4 (es) 1994-01-19
JPH06503174A (ja) 1994-04-07
DK0569494T3 (da) 1997-04-14
EP0569494B1 (en) 1997-03-26

Similar Documents

Publication Publication Date Title
NO872232D0 (no) Fettinversjons-gelelektroforese.
DE3687834D1 (de) Verfahren zum schnellen aufloesen von polymergelen.
DE3585172D1 (de) Strahlungsverfahren zur herstellung von elektrophoretischem gel.
DE3784240D1 (de) Gamma strahlensterilisierungsverfahren fuer polyvinylalkoholgel.
IT1230182B (it) Metodo ed apparato per riparare o fabbricare un oggetto.avente un supporto ed una sporgenza solidali
FI935772L (fi) Menetelmä etyleenipolymeerinkoostumuksen valmistamiseksi, etyleenipolymeerikoostumus ja sen käyttö
BR8501789A (pt) Matriz de extrusao com multiplos coletores e processo utilizando uma matriz de extrusao com multiplos coletores
FI883501A7 (fi) Menetelmä akryylipolymeerigeelin jatkuvaksi valmistamiseksi
ES2099249T3 (es) Gel binario mejorado de galactomanano-agarosa para electroforesis de acidos nucleicos.
FI955714A0 (fi) Parannettu menetelmä hydrofiilisten painovärien poistamiseksi
IT1242071B (it) Gel per la barba e metodo per prepararlo.
FR2724589B1 (fr) Procede et appareil de moulage par injection et soufflage
DE58903308D1 (de) Verfahren zur herstellung von 1-olefinpolymeren.
DE68914638D1 (de) Polyaluminosilikat-mikrogel, verfahren und zusammensetzung.
DE69025498D1 (de) Verfahren und Vorrichtung zur Übersetzung von Sätzen mit adverbialen Sätzen
EP0817717A4 (en) DEVICE AND METHOD FOR INJECTION MOLDING AND BLOW MOLDING
DE3778681D1 (de) Destillationsverfahren und vorrichtung.
IT1272087B (it) Metodo e macchina per la realizzazione di ruote dentate.
FI884096A7 (fi) Menetelmä ja laite suurikokoisten makromolekyylien erotteluun elektroforeesilla.
DE69633115D1 (de) Stromquelle und verfahren zum induktionserwärmen von gegenstanden
EP0585098A3 (en) Sign recognition device and method and sign translation system using it.
DE3689276D1 (de) Verfahren zur Aktualisierung der Informationen in einem Adressenübersetzungspufferspeicher.
DE58904328D1 (de) Verfahren zur desinfektion von medizinischen abformmassen.
EP0603820A3 (en) Method and device for making characters.
BR9607578A (pt) Método e composição polimerizável para proteção das lentes de matéria plástica dos faróis de veículos

Legal Events

Date Code Title Description
FG2A Definitive protection

Ref document number: 569494

Country of ref document: ES