ES2104616T3 - Procedimiento de adn recombinante y vectores de uso en el mismo. - Google Patents

Procedimiento de adn recombinante y vectores de uso en el mismo.

Info

Publication number
ES2104616T3
ES2104616T3 ES90916069T ES90916069T ES2104616T3 ES 2104616 T3 ES2104616 T3 ES 2104616T3 ES 90916069 T ES90916069 T ES 90916069T ES 90916069 T ES90916069 T ES 90916069T ES 2104616 T3 ES2104616 T3 ES 2104616T3
Authority
ES
Spain
Prior art keywords
gene
glutamine
vectors
recombinant dna
dna procedure
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
ES90916069T
Other languages
English (en)
Inventor
Christopher Robert Bebbington
Geoffrey Thomas Yarranton
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Rio Tinto Switzerland AG
Original Assignee
Alusuisse Holdings AG
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Alusuisse Holdings AG filed Critical Alusuisse Holdings AG
Application granted granted Critical
Publication of ES2104616T3 publication Critical patent/ES2104616T3/es
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells

Landscapes

  • Genetics & Genomics (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Saccharide Compounds (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Heat-Exchange Devices With Radiators And Conduit Assemblies (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

SE DESCRIBE UN METODO, PARA OBTENER UNA CELULA EUCARIOTICA QUE CONTIENE EN SU ADN MULTIPLES COPIAS DE UN GEN GS, QUE COMPRENDE: LA TRANSFORMACION DE UN AUXOTROPO DE GLUTAMINA EUCARIOTICO CON UN GEN GS; LA SELECCION DE CELULAS CAMBIANTES QUE CONTIENEN EL GEN GS; Y EL CULTIVO DE LAS CELULAS CAMBIANTES ELEGIDAS EN UN MEDIO QUE CARECE DE GLUTAMINA O EN EL QUE LA CANTIDAD DE GLUTAMINA SE REDUCE PROGRESIVAMENTE, CARACTERIZANDOSE EL GEN GS EN QUE, O LAS CONDICIONES EMPLEADAS DURANTE LAS FASES DEL CULTIVO SON TALES QUE, EL GEN GS SE COPIA TAN DEBILMENTE QUE SE ELIGEN LAS CELULAS EN LAS QUE SE HA AMPLIADO EL GEN GS.
ES90916069T 1989-10-25 1990-10-25 Procedimiento de adn recombinante y vectores de uso en el mismo. Expired - Lifetime ES2104616T3 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
GB898924021A GB8924021D0 (en) 1989-10-25 1989-10-25 Recombinant dna method and vectors for the use therein

Publications (1)

Publication Number Publication Date
ES2104616T3 true ES2104616T3 (es) 1997-10-16

Family

ID=10665141

Family Applications (1)

Application Number Title Priority Date Filing Date
ES90916069T Expired - Lifetime ES2104616T3 (es) 1989-10-25 1990-10-25 Procedimiento de adn recombinante y vectores de uso en el mismo.

Country Status (11)

Country Link
EP (1) EP0491875B1 (es)
JP (1) JP3073009B2 (es)
AT (1) ATE153383T1 (es)
AU (1) AU6629990A (es)
CA (1) CA2071543C (es)
DE (1) DE69030768T2 (es)
DK (1) DK0491875T3 (es)
ES (1) ES2104616T3 (es)
GB (1) GB8924021D0 (es)
GR (1) GR3023510T3 (es)
WO (1) WO1991006657A1 (es)

Families Citing this family (26)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7597886B2 (en) 1994-11-07 2009-10-06 Human Genome Sciences, Inc. Tumor necrosis factor-gamma
JP4503706B2 (ja) 1996-06-07 2010-07-14 ポニアード ファーマシューティカルズ，インコーポレイティド グリコシル化が改質されたヒト化抗体
EP1274720A4 (en) 2000-04-12 2004-08-18 Human Genome Sciences Inc FUSION PROTEINS CONTAINING ALBUMIN
US20030031675A1 (en) 2000-06-06 2003-02-13 Mikesell Glen E. B7-related nucleic acids and polypeptides useful for immunomodulation
WO2001096528A2 (en) 2000-06-15 2001-12-20 Human Genome Sciences, Inc. Human tumor necrosis factor delta and epsilon
DE60236646D1 (de) 2001-04-13 2010-07-22 Human Genome Sciences Inc Anti-VEGF-2 Antikörper
CN100594935C (zh) 2001-05-25 2010-03-24 人体基因组科学有限公司 免疫特异性结合trail受体的抗体
US20080194481A1 (en) 2001-12-21 2008-08-14 Human Genome Sciences, Inc. Albumin Fusion Proteins
PT1463751E (pt) 2001-12-21 2013-08-26 Human Genome Sciences Inc Proteínas de fusão de albumina
CA2475382A1 (en) 2002-02-07 2003-08-14 Delta Biotechnology Limited Albumin-fused anti-angiogenesis peptides
JP5010923B2 (ja) 2004-02-09 2012-08-29 ヒューマン ジノーム サイエンシーズ， インコーポレイテッド アルブミン融合蛋白質
EP1786463A4 (en) 2004-03-26 2009-05-20 Human Genome Sciences Inc ANTIBODY AGAINST NOGO RECEPTOR
US9505823B2 (en) 2006-08-07 2016-11-29 TEV A Biopharmaceuticals USA, Inc. Albumin-insulin fusion proteins
US9540426B2 (en) 2009-10-06 2017-01-10 Bristol-Myers Squibb Company Mammalian cell culture processes for protein production
EP2325322A1 (en) 2009-11-23 2011-05-25 4-Antibody AG Retroviral vector particles and methods for their generation and use
WO2012095514A1 (en) 2011-01-14 2012-07-19 Vivalis Recombinant protein production system
ES2676878T3 (es) 2011-03-03 2018-07-25 Zymeworks Inc. Diseño de armazón de heteromultímero multivalente y constructos
WO2013078118A1 (en) 2011-11-21 2013-05-30 Bristol-Myers Squibb Company Methods for determining the susceptibility of a virus to an attachment inhibitor
EP2848687B1 (en) 2012-04-27 2017-09-13 JCR Pharmaceuticals Co., Ltd. Novel expression vector
EP2674495A1 (en) * 2012-06-14 2013-12-18 Sanofi CHO expression system
AU2013289881B2 (en) 2012-07-13 2018-01-18 Zymeworks Bc Inc. Multivalent heteromultimer scaffold design and constructs
JP2015531244A (ja) 2012-10-15 2015-11-02 ブリストル−マイヤーズ スクイブ カンパニーＢｒｉｓｔｏｌ−Ｍｙｅｒｓ Ｓｑｕｉｂｂ Ｃｏｍｐａｎｙ タンパク質を製造するための哺乳動物細胞培養方法
AU2014262566A1 (en) 2013-05-08 2015-11-12 Zymeworks Inc. Bispecific HER2 and HER3 antigen binding constructs
CA2874083C (en) 2014-12-05 2024-01-02 Universite Laval Tdp-43-binding polypeptides useful for the treatment of neurodegenerative diseases
US10590457B2 (en) 2015-02-11 2020-03-17 Bristol Myers-Squibb Company Compositions for cell culture and methods of using the same
WO2017083224A1 (en) 2015-11-09 2017-05-18 Bristol-Myers Squibb Company Methods to manipulate quality attributes of polypeptides produced in cho cells

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB8601597D0 (en) * 1986-01-23 1986-02-26 Wilson R H Nucleotide sequences
EP0319206A3 (en) * 1987-11-30 1990-04-18 Berlex Laboratories, Inc. Gene amplification
GB8809129D0 (en) * 1988-04-18 1988-05-18 Celltech Ltd Recombinant dna methods vectors and host cells

Also Published As

Publication number Publication date
GR3023510T3 (en) 1997-08-29
ATE153383T1 (de) 1997-06-15
JPH05504050A (ja) 1993-07-01
GB8924021D0 (en) 1989-12-13
CA2071543A1 (en) 1991-04-26
EP0491875A1 (en) 1992-07-01
AU6629990A (en) 1991-05-31
WO1991006657A1 (en) 1991-05-16
DK0491875T3 (da) 1997-06-16
JP3073009B2 (ja) 2000-08-07
CA2071543C (en) 2005-02-22
EP0491875B1 (en) 1997-05-21
DE69030768D1 (de) 1997-06-26
DE69030768T2 (de) 1997-10-16

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