ES2106170T3 - Metodo para la extraccion de componentes intracelulares. - Google Patents

Metodo para la extraccion de componentes intracelulares.

Info

Publication number
ES2106170T3
ES2106170T3 ES92902694T ES92902694T ES2106170T3 ES 2106170 T3 ES2106170 T3 ES 2106170T3 ES 92902694 T ES92902694 T ES 92902694T ES 92902694 T ES92902694 T ES 92902694T ES 2106170 T3 ES2106170 T3 ES 2106170T3
Authority
ES
Spain
Prior art keywords
extractant
solution
intracellular components
extraction
cyclodextrin
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
ES92902694T
Other languages
English (en)
Inventor
Arne Lundin
John George Anson
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Merck Patent GmbH
Original Assignee
Merck Patent GmbH
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Merck Patent GmbH filed Critical Merck Patent GmbH
Application granted granted Critical
Publication of ES2106170T3 publication Critical patent/ES2106170T3/es
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/66—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving luciferase
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00—Chemistry: molecular biology and microbiology
    • Y10S435/81—Packaged device or kit
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
    • Y10T436/00—Chemistry: analytical and immunological testing
    • Y10T436/10—Composition for standardization, calibration, simulation, stabilization, preparation or preservation; processes of use in preparation for chemical testing
    • Y10T436/107497—Preparation composition [e.g., lysing or precipitation, etc.]

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Analytical Chemistry (AREA)
  • Biotechnology (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biophysics (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Medicines Containing Plant Substances (AREA)

Abstract

UN METODO PARA PREPARAR UN EXTRACTO DE UN COMPONENTE INTRACELULAR DONDE LAS CELULAS SON CONTACTADAS CON UN EXTACTANTE PARA GENERAR UNA SOLUCION DE EXTRACTO, SE CARACTERIZA POR CONTACTAR LA SOLUCION CON UNA CICLODEXTRINA PARA NEUTRALIZAR EL EXTRACTANTR.LOS MEJORES EXTRACTANTES SON LOS CATIONICOS O DE OTRO TIPO.LA CICLODEXTRINA SE UTILIZA PREFERENTEMENTE EN SOLUCION.LOS MEJORES COMPONENTES INTRACELULARES SON LOS ATP, LOS CUALES PUEDEN, TRAS LA NEUTRALIZACION DE LOS EXTACTANTES,PASAR POR UN ENSAYO REGULADOR DE LLAMA DE LUCIFERASA; Y LOS ADN O ARN QUE PUEDEN, TRAS LA NEUTRALIZACION DEL SURFACTANTE,SER AMPLIADOS O POSTERIORMENTE PROCESADOS DE OTRAS FORMAS.
ES92902694T 1991-01-10 1992-01-10 Metodo para la extraccion de componentes intracelulares. Expired - Lifetime ES2106170T3 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
GB919100551A GB9100551D0 (en) 1991-01-10 1991-01-10 Method and reagent for eliminating analytical interference in enzymatic analysis from substances used for extraction of intracellular metabolites

Publications (1)

Publication Number Publication Date
ES2106170T3 true ES2106170T3 (es) 1997-11-01

Family

ID=10688253

Family Applications (1)

Application Number Title Priority Date Filing Date
ES92902694T Expired - Lifetime ES2106170T3 (es) 1991-01-10 1992-01-10 Metodo para la extraccion de componentes intracelulares.

Country Status (9)

Country Link
US (1) US5558986A (es)
EP (1) EP0566625B1 (es)
JP (1) JP3140057B2 (es)
AT (1) ATE156861T1 (es)
DE (1) DE69221604T2 (es)
DK (1) DK0566625T3 (es)
ES (1) ES2106170T3 (es)
GB (1) GB9100551D0 (es)
WO (1) WO1992012253A1 (es)

Families Citing this family (33)

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Publication number Priority date Publication date Assignee Title
US5705345A (en) * 1991-01-10 1998-01-06 Amersham International Plc Methods and kits for preparing nucleic acids using cyclodextrin
US5538870A (en) * 1994-09-21 1996-07-23 Boehringer Mannheim Corporation Method for preparing nucleic acids for analysis and kits useful therefor
JP3118528B2 (ja) 1996-01-26 2000-12-18 東邦レーヨン株式会社 ステアリングホイールおよびその製造方法
US5905029A (en) * 1997-02-19 1999-05-18 Fritz Berthold Method for rapid hygiene testing
US5916802A (en) * 1997-02-19 1999-06-29 Fritz Berthold Device for measuring ATP
DE69831522T2 (de) 1997-03-03 2006-06-14 Amersham Biosciences Uk Ltd In-situ Zellextraktion und Assay-Verfahren
JP3773151B2 (ja) 1997-12-03 2006-05-10 キッコーマン株式会社 細胞内成分の分析法
JP3749628B2 (ja) 1997-12-26 2006-03-01 キッコーマン株式会社 ルシフェラーゼおよびそれを用いる細胞内ａｔｐの測定法
JP4616990B2 (ja) * 1998-02-02 2011-01-19 キアジエン・ノース・アメリカン・ホールデイングス・インコーポレーテツド Ｄｎａを単離し、増幅し、そして特性化するための方法
DE69935850T2 (de) * 1998-02-16 2008-01-10 Ge Healthcare Uk Ltd., Little Chalfont Verfahren zur messung der gesamkonzentration eines analytes
US20050089918A1 (en) * 1998-02-23 2005-04-28 Amersham Biosciences Uk Limited In-situ cell extraction and assay method
DE19838429C2 (de) * 1998-08-24 2001-07-26 Fraunhofer Ges Forschung Anordnung zum schnellen Nachweis von mikrobiologischen oder biochemischen Stoffen
NL1010224C2 (nl) * 1998-09-30 2000-03-31 Packard Biosciene B V Werkwijze voor het detecteren van ATP.
US6653147B2 (en) 2000-03-31 2003-11-25 Neogen Corporation Apparatus and method for chemiluminescent assays
US6927851B2 (en) 2000-03-31 2005-08-09 Neogen Corporation Methods and apparatus to improve the sensitivity and reproducibility of bioluminescent analytical methods
US6660489B2 (en) * 2000-12-01 2003-12-09 Becton, Dickinson And Company ATP extraction method
US7083911B2 (en) * 2001-02-16 2006-08-01 Promega Corporation Method for detection of ATP
JP2005502363A (ja) * 2001-09-11 2005-01-27 メルク　パテント　ゲゼルシャフト　ミット　ベシュレンクテル　ハフトング 酵素アッセイのためのラテラルフローテストフォーマット
AU2002348287A1 (en) * 2001-11-16 2003-06-10 Pierce Biotechnology, Inc. Phosphine-containing formulations for chemiluminescent luciferase assays
BRPI0214680B1 (pt) 2001-12-06 2018-09-18 Biocontrol Systems, Inc. instrumento para uso em monitorar um produto, um ingrediente, um ambiente ou processo, instrumento para detectar emissão de luz de uma amostra e método de monitorar uma amostra de um produto, um ingrediente, um processo ou ambiente
US20030209653A1 (en) * 2002-04-24 2003-11-13 Biocontrol Systems, Inc. Sample collection and testing system
WO2005019235A2 (en) * 2003-08-15 2005-03-03 Celsis Inc. Use of novel compounds to release nucleotides from living cells
US20050070701A1 (en) * 2003-09-29 2005-03-31 Hochstetler Spencer Erich Detection of living cells in polymers or pigments
US7422868B2 (en) * 2004-07-02 2008-09-09 Promega Corporation Microbial ATP extraction and detection system
US7556933B2 (en) * 2004-10-01 2009-07-07 Luminultra Technologies Ltd. Reagent system and process for adenosine triphosphate monitoring
EP1955077B1 (en) 2005-12-02 2012-06-13 Sirtris Pharmaceuticals, Inc. Mass spectrometry assays for acetyltransferase/deacetylase activity
WO2008045121A1 (en) * 2006-10-13 2008-04-17 Charm Sciences, Inc. Method and apparatus for reducing luminescent test result interferences
US7829280B2 (en) * 2007-01-25 2010-11-09 Luminultra Technologies Ltd. Process for reducing interferences in luciferase enzyme assays
US20100116735A1 (en) * 2008-11-13 2010-05-13 Yu F Philip Method of monitoring and controlling biological activity in boiler condensate and feedwater systems
US20110256592A1 (en) * 2008-12-12 2011-10-20 Eurogentec S.A Use of cyclodextrins to improve the specificity, sensitivity and yield of nucleic acid amplification reactions
US9446406B2 (en) 2012-06-29 2016-09-20 Biocontrol Systems, Inc. Sample collection and bioluminescent analysis system
US20160010139A1 (en) * 2013-03-06 2016-01-14 Ruhof Corporation Devices and methods for testing the cleanliness of medical instruments
EP4317451A4 (en) * 2021-04-01 2025-03-12 Fujirebio Inc. Method for treating biological sample

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB1604249A (en) * 1977-05-31 1981-12-02 Kolehmainen S Selective measurement of somatic and microbial cells
US4956274A (en) * 1987-04-06 1990-09-11 Microgenics Corporation Reagent stabilization in enzyme-donor and acceptor assay
EP0301847A1 (en) * 1987-07-29 1989-02-01 Microgenics Corporation Cleaning using cyclodextrin
GB8722252D0 (en) * 1987-09-22 1987-10-28 Brewing Res Found Atp extraction
US5032503A (en) * 1988-06-22 1991-07-16 Microgenics Corporation Liquid single reagent for air enzyme complementation assay
US5231015A (en) * 1989-10-18 1993-07-27 Eastman Kodak Company Methods of extracting nucleic acids and pcr amplification without using a proteolytic enzyme

Also Published As

Publication number Publication date
DE69221604D1 (de) 1997-09-18
WO1992012253A1 (en) 1992-07-23
JPH06504200A (ja) 1994-05-19
DK0566625T3 (da) 1998-03-09
EP0566625B1 (en) 1997-08-13
JP3140057B2 (ja) 2001-03-05
GB9100551D0 (en) 1991-02-20
EP0566625A1 (en) 1993-10-27
DE69221604T2 (de) 1998-03-12
ATE156861T1 (de) 1997-08-15
US5558986A (en) 1996-09-24

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