ES2107997T3 - Procedimiento de uso de vectores de expresion eucarioticos que comprenden el potenciador del virus bk. - Google Patents

Procedimiento de uso de vectores de expresion eucarioticos que comprenden el potenciador del virus bk.

Info

Publication number
ES2107997T3
ES2107997T3 ES87303083T ES87303083T ES2107997T3 ES 2107997 T3 ES2107997 T3 ES 2107997T3 ES 87303083 T ES87303083 T ES 87303083T ES 87303083 T ES87303083 T ES 87303083T ES 2107997 T3 ES2107997 T3 ES 2107997T3
Authority
ES
Spain
Prior art keywords
improver
present
euchariotic
tandem
expression vectors
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
ES87303083T
Other languages
English (en)
Inventor
Brian William Grinnel
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Eli Lilly and Co
Original Assignee
Eli Lilly and Co
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Eli Lilly and Co filed Critical Eli Lilly and Co
Application granted granted Critical
Publication of ES2107997T3 publication Critical patent/ES2107997T3/es
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
    • C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
    • C12N9/6424—Serine endopeptidases (3.4.21)
    • C12N9/6464—Protein C (3.4.21.69)
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P7/00—Drugs for disorders of the blood or the extracellular fluid
    • A61P7/02—Antithrombotic agents; Anticoagulants; Platelet aggregation inhibitors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
    • C12Y304/21—Serine endopeptidases (3.4.21)
    • C12Y304/21069—Protein C activated (3.4.21.69)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2799/00—Uses of viruses
    • C12N2799/02—Uses of viruses as vector
    • C12N2799/021—Uses of viruses as vector for the expression of a heterologous nucleic acid
    • C12N2799/022—Uses of viruses as vector for the expression of a heterologous nucleic acid where the vector is derived from an adenovirus
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2830/00—Vector systems having a special element relevant for transcription
    • C12N2830/001—Vector systems having a special element relevant for transcription controllable enhancer/promoter combination
    • C12N2830/002—Vector systems having a special element relevant for transcription controllable enhancer/promoter combination inducible enhancer/promoter combination, e.g. hypoxia, iron, transcription factor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2830/00—Vector systems having a special element relevant for transcription
    • C12N2830/30—Vector systems having a special element relevant for transcription being an enhancer not forming part of the promoter region
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2830/00—Vector systems having a special element relevant for transcription
    • C12N2830/42—Vector systems having a special element relevant for transcription being an intron or intervening sequence for splicing and/or stability of RNA
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2830/00—Vector systems having a special element relevant for transcription
    • C12N2830/55—Vector systems having a special element relevant for transcription from bacteria
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2830/00—Vector systems having a special element relevant for transcription
    • C12N2830/60—Vector systems having a special element relevant for transcription from viruses
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2830/00—Vector systems having a special element relevant for transcription
    • C12N2830/80—Vector systems having a special element relevant for transcription from vertebrates
    • C12N2830/85—Vector systems having a special element relevant for transcription from vertebrates mammalian

Landscapes

  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Diabetes (AREA)
  • Physics & Mathematics (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Veterinary Medicine (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Hematology (AREA)
  • Public Health (AREA)
  • Animal Behavior & Ethology (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Saccharide Compounds (AREA)
  • Peptides Or Proteins (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)

Abstract

LA PRESENTE INVENCION ES UN METODO DE UTILIZAR MEJORADOR BK EN TANDEM CON UN FAVORECEDOR EUCARIOTICO PARA FAVORECER LA TRANSCRIPCION DEL ADN QUE CODIFICA A UNA SUSTANCIA UTIL. EL METODO DE LA PRESENTE INVENCION REQUIERE LA PRESENCIA DEL PRODUCTO DE GEN E1A PARA LA MAXIMA EXPRESION DE LA SUSTANCIA UTIL. LA PRESENTE INVENCION COMPRENDE TAMBIEN VARIOS VECTORES DE EXPRESION UTILES QUE COMPRENDEN EL MEJORADOR BK EN TANDEM CON EL ADENOVIRUS 2, ULTIMO FAVORECEDOR POSICIONADO PARA ESTIMULAR LA EXPRESION DE UNA DIVERSIDAD DE PROTEINAS, TALES COMO PROTEINA G, CLORANFENICOL ACETILTRANSFERASA Y ACTIVADOR DE PLASMINOGENO TISULAR. LA PRESENTE INVENCION COMPRENDE, ADEMAS, UN METODO PARA INCREMENTAR LA ACTIVIDAD DEL MEJORADOR BK, QUE IMPLICA LA COLOCACION DEL MEJORADOR BK INMEDIATAMENTE AGUAS ARRIBA DEL FAVORECEDOR EUCARIOTICO UTILIZADO EN TANDEM CON EL MEJORADOR BK PARA ESTIMULAR LA EXPRESION DE UNA SUSTANCIA UTIL.
ES87303083T 1986-04-09 1987-04-09 Procedimiento de uso de vectores de expresion eucarioticos que comprenden el potenciador del virus bk. Expired - Lifetime ES2107997T3 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US84999986A 1986-04-09 1986-04-09

Publications (1)

Publication Number Publication Date
ES2107997T3 true ES2107997T3 (es) 1997-12-16

Family

ID=25307027

Family Applications (3)

Application Number Title Priority Date Filing Date
ES87303083T Expired - Lifetime ES2107997T3 (es) 1986-04-09 1987-04-09 Procedimiento de uso de vectores de expresion eucarioticos que comprenden el potenciador del virus bk.
ES95202327T Expired - Lifetime ES2237755T3 (es) 1986-04-09 1987-04-09 Procedimiento de uso de vectores de expresion eucariotas que comprenden el potenciador del virus bk.
ES93201077T Expired - Lifetime ES2227514T3 (es) 1986-04-09 1987-04-09 Procedimiento de uso de vectores de expresion eurocariotica que comprende un estimulante del virus bk.

Family Applications After (2)

Application Number Title Priority Date Filing Date
ES95202327T Expired - Lifetime ES2237755T3 (es) 1986-04-09 1987-04-09 Procedimiento de uso de vectores de expresion eucariotas que comprenden el potenciador del virus bk.
ES93201077T Expired - Lifetime ES2227514T3 (es) 1986-04-09 1987-04-09 Procedimiento de uso de vectores de expresion eurocariotica que comprende un estimulante del virus bk.

Country Status (10)

Country Link
EP (3) EP0576049A1 (es)
JP (5) JP3162056B2 (es)
AU (1) AU605479B2 (es)
CA (2) CA1318619C (es)
DE (3) DE3752134T2 (es)
DK (1) DK176387A (es)
ES (3) ES2107997T3 (es)
HU (1) HU211391B (es)
IE (3) IE81065B1 (es)
IL (5) IL98887A (es)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB8613481D0 (en) * 1986-06-04 1986-07-09 Diatech Ltd Translation of mrna
EP3020808B1 (en) * 2013-07-11 2019-09-11 Takara Bio Inc. Method for manufacturing adeno-associated virus
EP3105311A1 (en) 2014-02-10 2016-12-21 Univercells NV System, apparatus and method for biomolecules production
EP3168298B1 (en) 2014-07-10 2019-04-17 Takara Bio Inc. Production method for non-enveloped virus particles
WO2016111343A1 (ja) 2015-01-09 2016-07-14 タカラバイオ株式会社 非エンベロープウイルス粒子の製造方法

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB8327860D0 (en) * 1983-10-18 1983-11-16 Fujisawa Pharmaceutical Co Monoclonal antiprotein c antibody
JPH0789930B2 (ja) * 1984-03-08 1995-10-04 大正製薬株式会社 ウイルス性転写促進配列
US4775624A (en) * 1985-02-08 1988-10-04 Eli Lilly And Company Vectors and compounds for expression of human protein C
EP0349598A1 (en) * 1987-03-13 1990-01-10 Schering Corporation Method for stimulating gene expression
JPH0660120A (ja) * 1992-08-06 1994-03-04 Sharp Corp 可変長データの格納方法及び検索装置

Also Published As

Publication number Publication date
HK1009605A1 (en) 1999-06-04
DE3752134T2 (de) 1998-03-19
IE870912L (en) 1987-10-09
DK176387A (da) 1987-11-13
HK1021861A1 (en) 2000-07-07
EP0576049A1 (en) 1993-12-29
JPS6312296A (ja) 1988-01-19
ES2237755T3 (es) 2005-08-01
IL98887A (en) 1992-12-01
DE3752382D1 (de) 2005-04-14
CA1318619C (en) 1993-06-01
ES2227514T3 (es) 2005-04-01
DE3752382T2 (de) 2005-12-29
AU7117587A (en) 1987-10-15
IL82093A (en) 1992-12-01
HUT43633A (en) 1987-11-30
HU211391B (en) 1995-11-28
IE980258A1 (en) 1987-10-09
EP0699764B1 (en) 2005-03-09
EP0584845A1 (en) 1994-03-02
IL98886A0 (en) 1992-07-15
JPH11346789A (ja) 1999-12-21
JPH099980A (ja) 1997-01-14
DE3752376D1 (de) 2004-10-14
DE3752134D1 (de) 1997-12-04
JP2877228B2 (ja) 1999-03-31
AU605479B2 (en) 1991-01-17
JP2958286B2 (ja) 1999-10-06
IL98887A0 (en) 1992-07-15
IL82093A0 (en) 1987-10-30
IE980257A1 (en) 1987-10-09
JPH1057081A (ja) 1998-03-03
IL98886A (en) 1992-12-01
EP0584845B1 (en) 2004-09-08
DK176387D0 (da) 1987-04-07
IE81065B1 (en) 2000-01-12
JP2001145496A (ja) 2001-05-29
EP0699764A1 (en) 1996-03-06
JP3162056B2 (ja) 2001-04-25
CA1340384C (fr) 1999-02-09

Similar Documents

Publication Publication Date Title
EP0776331A4 (en) HIGHER ORDER STRUCTURE AND BINDING OF PEPTIDE NUCLEIC ACIDS
ES8703930A1 (es) Procedimiento de preparar hirudina y analogos a partir de levadura
BR9713294A (pt) "anticorpos contra proteìna relacionada a hormÈnio de paratiróide humano"
ES2038579T1 (es) Una celula de levadura del genero schwanniovices.
MY102472A (en) Preparation of funtional human urokinase proteins.
EP1009814A4 (en) KDR, A HUMAN TYROSIN KINASE RECEPTOR
DK1394179T3 (da) Fremgangsmåde til fremstilling af erythropoietin fri for animalske proteiner
ES2147553T3 (es) Vectores virales recombinantes para la expresion en unas celulas musculares.
ATE298371T1 (de) Nicht-primaten lentivirale vektoren und verpackungssysteme
NO20013701D0 (no) HER-2/neu-fusjonsproteiner
ES2191011T3 (es) Acido nucleico, vector de expresion y composiciones para la identificacion y sintesis de sialiltransferasas recombinantes.
ATE426024T1 (de) C-c chemokin rezeptor 3: ckr-3 oder eos-l2
NO960309L (no) Agonister og antagonister for humaninterleukin-10
ES2062993T3 (es) Factor de crecimiento humano i semejante a la preproinsulina.
AU592749B2 (en) Cloning of dna for protozoal antigens
ES2010727A6 (es) Metodo para producir lineas celulares que producen anticuerpos monoclonales reactivos frente a determinantes antigenicos de gp110 o de p25 de hiv.
ES2104596T3 (es) Nuevos polipeptidos, secuencias de adn que permiten su expresion, metodo de preparacion, y utilizacion.
ES2054612T3 (es) Secuencias de adn que codifican para proteinas con actividad biologica de los inhibidores husi tipo-i y procedimientos tecnologicos geneticos para la preparacion de estas proteinas.
AR241794A1 (es) Procedimiento para obtener inteerleucina-2 humana, en una celula de ovario de hamster de la china.
CA1341437C (en) METHOD OF USING EUKARYOTIC EXPRESSION VECTORS COMPRISING A BK VIRUS AMPLIFIER
HUP9901035A2 (hu) Idegen DNS-t tartalmazó parapoxvírusok, előállításuk és alkalmazásuk vakcinákban
PE50997A1 (es) Producto del gen de la obesidad
ES2107997T3 (es) Procedimiento de uso de vectores de expresion eucarioticos que comprenden el potenciador del virus bk.
TR199701394T1 (xx) Gen ekspresyonu i�in promot�rler
DE69830910D1 (de) Kaliumkanal-proteine zur verwendung in gentherapie zur verminderung von erektiler dysfunktion

Legal Events

Date Code Title Description
FG2A Definitive protection

Ref document number: 245949

Country of ref document: ES