ES2113873T3 - Aparato de electroforesis en gel horizontal. - Google Patents

Aparato de electroforesis en gel horizontal.

Info

Publication number
ES2113873T3
ES2113873T3 ES91307761T ES91307761T ES2113873T3 ES 2113873 T3 ES2113873 T3 ES 2113873T3 ES 91307761 T ES91307761 T ES 91307761T ES 91307761 T ES91307761 T ES 91307761T ES 2113873 T3 ES2113873 T3 ES 2113873T3
Authority
ES
Spain
Prior art keywords
gel
gel electrophoresis
electrophoresis apparatus
sample introduction
horizontal gel
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
ES91307761T
Other languages
English (en)
Inventor
Robert L Brumley Jr
Lloyd M Smith
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Wisconsin Alumni Research Foundation
Original Assignee
Wisconsin Alumni Research Foundation
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Wisconsin Alumni Research Foundation filed Critical Wisconsin Alumni Research Foundation
Application granted granted Critical
Publication of ES2113873T3 publication Critical patent/ES2113873T3/es
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/416—Systems
    • G01N27/447—Systems using electrophoresis
    • G01N27/44704—Details; Accessories
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/416—Systems
    • G01N27/447—Systems using electrophoresis
    • G01N27/44756—Apparatus specially adapted therefor

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Molecular Biology (AREA)
  • Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Electrochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Peptides Or Proteins (AREA)
  • Colloid Chemistry (AREA)
  • Sampling And Sample Adjustment (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Devices For Indicating Variable Information By Combining Individual Elements (AREA)
  • Electrochromic Elements, Electrophoresis, Or Variable Reflection Or Absorption Elements (AREA)

Abstract

SE DESCRIBE UN CONJUNTO DE ELECTROFORESIS HORIZONTAL DISEÑADO PARA UTILIZAR GELES MUY FINOS DE POLIACRILAMIDA. EL CONJUNTO PERMITE LA REFRIGERACION POR AGUA DEL GEL DURANTE LA ELECTROFORESIS PARA QUE SE PUEDAN APLICAR ALTOS VOLTAJES AL GEL SIN DEGRADACION DE POLIMEROS. EL MOLDE DE GEL SE ELABORA A PARTIR DE UN JUEGO DE PLACAS DE VIDRIO SUJETADOS EN SU LUGAR DEFINIENDO UN MOLDE DE GEL ENTRE ELLAS. EN UNA EJEMPLIFICACION, SE SEPARA EL SITIO DE INTRODUCCION DE CAMPO DEL SITIO DE INTRODUCCION DE MUESTRA DE MANERA QUE EL CAMPO ELECTRICO SEA GENERALMENTE LINEAL EN LA ZONA DE INTRODUCCION DE LA MUESTRA.
ES91307761T 1990-12-20 1991-08-22 Aparato de electroforesis en gel horizontal. Expired - Lifetime ES2113873T3 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US07/630,675 US5137613A (en) 1990-12-20 1990-12-20 Horizontal gel electrophoresis apparatus

Publications (1)

Publication Number Publication Date
ES2113873T3 true ES2113873T3 (es) 1998-05-16

Family

ID=24528123

Family Applications (1)

Application Number Title Priority Date Filing Date
ES91307761T Expired - Lifetime ES2113873T3 (es) 1990-12-20 1991-08-22 Aparato de electroforesis en gel horizontal.

Country Status (7)

Country Link
US (1) US5137613A (es)
EP (1) EP0492769B1 (es)
JP (1) JPH04335152A (es)
AT (1) ATE161632T1 (es)
CA (1) CA2049749A1 (es)
DE (1) DE69128522T2 (es)
ES (1) ES2113873T3 (es)

Families Citing this family (18)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5242568A (en) * 1992-01-14 1993-09-07 Fotodyne Incorporated Electrophoresis apparatus
US5338426A (en) * 1992-08-28 1994-08-16 Applied Biosystems, Inc. Horizontal polyacrylamide gel electrophoresis
US5449446A (en) * 1994-03-09 1995-09-12 Verma; Sumeet Multi-purpose electrophoresis apparatus
US5507934A (en) * 1994-11-01 1996-04-16 Visible Genetics Inc. Apparatus for preparing gels for use in electrophoretic separations and similar applications
US6017434A (en) 1995-05-09 2000-01-25 Curagen Corporation Apparatus and method for the generation, separation, detection, and recognition of biopolymer fragments
US6485625B1 (en) 1995-05-09 2002-11-26 Curagen Corporation Apparatus and method for the generation, separation, detection, and recognition of biopolymer fragments
US5549806A (en) * 1996-02-21 1996-08-27 Chen; Stephen L. Device and method of direct water cooling for horizontal submarine gel electrophoresis
US5759375A (en) * 1996-05-17 1998-06-02 Purdue Research Foundation Miniaturized disposable gels for DNA analysis
US6207354B1 (en) 1999-04-07 2001-03-27 International Business Machines Coporation Method of making an organic chip carrier package
US20010037940A1 (en) * 2000-01-28 2001-11-08 Lih-Bin Shih Apparatus for horizontal gel electrophoresis and method of using same
JP2002048765A (ja) * 2000-08-03 2002-02-15 Masabumi Youda 水平面型ゲル電気泳動装置用試料導入装置、水平面型ゲル電気泳動システム、その試料導入方法およびその試料採取方法
WO2002055189A2 (en) * 2001-01-12 2002-07-18 Syngenta Participations Ag Nanoporous membrane reactor for miniaturized reactions and enhanced reaction kinetics
US20020127148A1 (en) * 2001-03-09 2002-09-12 Sanjay Kumar Gel processing and transfer device
AUPR800701A0 (en) * 2001-09-28 2001-10-25 Proteome Systems Ltd Cassette for electrophoresis
EP1353172B1 (de) * 2002-04-12 2006-03-15 Tecan Trading AG Kassette, System und 2D-Gelelektrophorese-Verfahren zum Trennen von Molekülen
US7303715B1 (en) 2002-12-04 2007-12-04 Owl Separation Systems, Inc. Horizontal gel casting device and method
US20040171167A1 (en) * 2002-12-13 2004-09-02 Affymetrix, Inc. Chip-in-a-well scanning
US20080164149A1 (en) * 2007-01-05 2008-07-10 Artz Matthew R Rapid gel electrophoresis system

Family Cites Families (19)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3888759A (en) * 1973-05-25 1975-06-10 Yeda Res & Dev Flat plate electrophoresis
JPS5416281A (en) * 1978-04-07 1979-02-06 Fuji Glass Method of melting and closing branch tube of ampule
DE3024288C2 (de) * 1980-06-27 1982-03-25 Europäisches Laboratorium für Molekularbiologie (EMBL), 6900 Heidelberg Verfahren und Vorrichtung zur Herstellung dünner Schichten
DE3046729C2 (de) * 1980-12-11 1985-05-15 Europäisches Laboratorium für Molekularbiologie (EMBL), 6900 Heidelberg Thermostatische Platte für eine Elektrophoreseeinrichtung
FR2496889A1 (fr) * 1980-12-22 1982-06-25 Hours Michel Cuve de migration et de reaction utilisable pour des analyses par electrophorese
US4588491A (en) * 1984-02-27 1986-05-13 International Biotechnologies, Inc. Horizontal gel electrophoresis device
US4693804A (en) * 1984-12-19 1987-09-15 Board Of Regents, The University Of Texas System Apparatus for bidimensional electrophoretic separations
US4612106A (en) * 1984-12-24 1986-09-16 Kromer Heiner M Cooling system for a slab gel electrophoresis apparatus
US4608146A (en) * 1985-04-09 1986-08-26 Bio-Rad Laboratories, Inc. Horizontal electrophoresis cell for rapid assembly
US4756809A (en) * 1986-02-04 1988-07-12 Oncor, Inc. Process for conducting electrophoresis and transfer
US4849078A (en) * 1986-09-25 1989-07-18 Oncor, Inc. Process for conducting electrophoresis and transfer
US4773984A (en) * 1987-02-02 1988-09-27 Life Technologies, Inc. Vertical gel slab electrophoresis apparatus
US4909977A (en) * 1987-05-20 1990-03-20 Bios Corporation Method for molding thin gel slabs horizontally with integrally molded large volume sample wells
US4795541A (en) * 1987-05-20 1989-01-03 Bios Corporation Method and apparatus for molding thin gel slabs horizontally with integrally molded large volume sample wells
JPS63317756A (ja) * 1987-06-19 1988-12-26 Fuji Photo Film Co Ltd 電気泳動装置
US4865714A (en) * 1987-07-17 1989-09-12 Beckman Instruments, Inc. Electrophoretic gel cooled cell
US4913791A (en) * 1987-08-28 1990-04-03 Bios Corporation Blot frame and method of handling
US4830726A (en) * 1988-02-03 1989-05-16 The Wistar Institute Separation of large DNA molecules in alternating asymmetric electric fields
US4919784A (en) * 1989-08-16 1990-04-24 Eg&G, Inc. Electrophorresis cassette spacer

Also Published As

Publication number Publication date
CA2049749A1 (en) 1992-06-21
DE69128522D1 (de) 1998-02-05
ATE161632T1 (de) 1998-01-15
DE69128522T2 (de) 1998-08-13
EP0492769A2 (en) 1992-07-01
EP0492769A3 (en) 1993-11-18
JPH04335152A (ja) 1992-11-24
US5137613A (en) 1992-08-11
EP0492769B1 (en) 1997-12-29

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