ES2129241T3 - Un aparato para realizar una reaccion por ciclos magneticos. - Google Patents

Un aparato para realizar una reaccion por ciclos magneticos.

Info

Publication number
ES2129241T3
ES2129241T3 ES95943789T ES95943789T ES2129241T3 ES 2129241 T3 ES2129241 T3 ES 2129241T3 ES 95943789 T ES95943789 T ES 95943789T ES 95943789 T ES95943789 T ES 95943789T ES 2129241 T3 ES2129241 T3 ES 2129241T3
Authority
ES
Spain
Prior art keywords
reaction
perform
nucleic acid
amplification
magnetic cycles
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
ES95943789T
Other languages
English (en)
Inventor
Alec Mian
Stephen G Kieffer-Higgins
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Gamera Bioscience Corp
Original Assignee
Gamera Bioscience Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Gamera Bioscience Corp filed Critical Gamera Bioscience Corp
Application granted granted Critical
Publication of ES2129241T3 publication Critical patent/ES2129241T3/es
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B03—SEPARATION OF SOLID MATERIALS USING LIQUIDS OR USING PNEUMATIC TABLES OR JIGS; MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS; SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS
    • B03C—MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS; SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS
    • B03C1/00—Magnetic separation
    • B03C1/02—Magnetic separation acting directly on the substance being separated
    • B03C1/035—Open gradient magnetic separators, i.e. separators in which the gap is unobstructed, characterised by the configuration of the gap
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B03—SEPARATION OF SOLID MATERIALS USING LIQUIDS OR USING PNEUMATIC TABLES OR JIGS; MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS; SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS
    • B03C—MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS; SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS
    • B03C1/00—Magnetic separation
    • B03C1/02—Magnetic separation acting directly on the substance being separated
    • B03C1/28—Magnetic plugs and dipsticks
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6846—Common amplification features
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/686—Polymerase chain reaction [PCR]
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • G01N35/0098—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor involving analyte bound to insoluble magnetic carrier, e.g. using magnetic separation
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
    • Y10T436/00—Chemistry: analytical and immunological testing
    • Y10T436/20—Oxygen containing
    • Y10T436/206664—Ozone or peroxide
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
    • Y10T436/00—Chemistry: analytical and immunological testing
    • Y10T436/20—Oxygen containing
    • Y10T436/207497—Molecular oxygen

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Analytical Chemistry (AREA)
  • Biotechnology (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Immunology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
  • Liquid Crystal (AREA)
  • Analysing Materials By The Use Of Radiation (AREA)
  • Hard Magnetic Materials (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Heterocyclic Carbon Compounds Containing A Hetero Ring Having Nitrogen And Oxygen As The Only Ring Hetero Atoms (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)

Abstract

LA INVENCION SE REFIERE A UN APARATO PARA REALIZAR UN PROCESO PARA LA AMPLIFICACION DE SECUENCIAS ESPECIFICAS DE ACIDO NUCLEICO BASADO EN LA SEPARACION DE HEBRAS DE ACIDO NUCLEICO MEDIANTE UN CAMPO ELECTROMAGNETICO. ESTE METODO DE SEPARACION PERMITE EL USO DE POLIMERASAS MESOFILICAS EN EL PROCESO DE AMPLIFICACION, AUMENTANDO ASI LA VELOCIDAD Y FIDELIDAD DEL PROCESO DE AMPLIFICACION, ASI COMO EL TAMAÑO DEL ACIDO NUCLEICO OBJETIVO QUE SE PUEDE AMPLIFICAR.
ES95943789T 1994-12-09 1995-12-08 Un aparato para realizar una reaccion por ciclos magneticos. Expired - Lifetime ES2129241T3 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US08/353,573 US5686271A (en) 1994-06-09 1994-12-09 Apparatus for performing magnetic cycle reaction

Publications (1)

Publication Number Publication Date
ES2129241T3 true ES2129241T3 (es) 1999-06-01

Family

ID=23389720

Family Applications (1)

Application Number Title Priority Date Filing Date
ES95943789T Expired - Lifetime ES2129241T3 (es) 1994-12-09 1995-12-08 Un aparato para realizar una reaccion por ciclos magneticos.

Country Status (11)

Country Link
US (1) US5686271A (es)
EP (1) EP0796345B1 (es)
JP (1) JPH10510158A (es)
CN (1) CN1175282A (es)
AT (1) ATE178098T1 (es)
AU (1) AU691076B2 (es)
CA (1) CA2207388A1 (es)
DE (1) DE69508617T2 (es)
DK (1) DK0796345T3 (es)
ES (1) ES2129241T3 (es)
WO (1) WO1996017959A2 (es)

Families Citing this family (34)

* Cited by examiner, † Cited by third party
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GB9620934D0 (en) 1996-10-08 1996-11-27 Molecular Drives Limited Multi-well containers
US5916776A (en) * 1997-08-27 1999-06-29 Sarnoff Corporation Amplification method for a polynucleotide
EP1020534B2 (en) * 1998-11-09 2011-01-05 Eiken Kagaku Kabushiki Kaisha Process for synthesizing nucleic acid
DE19907470A1 (de) * 1999-02-12 2000-08-17 Thomas Lurz Verfahren zur Auftrennung von in Lösung befindlichen doppelsträngigen Nukleinsäuren in einzelsträngige Nukleinsäuren
US20040129555A1 (en) * 1999-04-09 2004-07-08 Marchitto Kevin S. Energy enhanced reaction catalysis and uses thereof
US7088650B1 (en) 1999-08-23 2006-08-08 Worthington Mark O Methods and apparatus for optical disc data acquisition using physical synchronization markers
EP1134294A3 (en) * 2000-03-16 2004-06-30 Kabushiki Kaisha Toshiba Method for producing immobilized nucleic acid strand
US6672458B2 (en) * 2000-05-19 2004-01-06 Becton, Dickinson And Company System and method for manipulating magnetically responsive particles fluid samples to collect DNA or RNA from a sample
ES2319101T3 (es) * 2000-05-19 2009-05-04 Becton Dickinson And Company Sistema y procedimiento de manipulacion de particulas magneticamente sensibles en muestras de fluido para extraer el adn o el arn de una muestra.
DE10036486A1 (de) * 2000-07-25 2002-02-14 Biotix Gmbh Verfahren zur Auftrennung von in Lösung befindlichen doppelstränigen Nukleinsäuren in einzelsträngige Nukleinsäuren
US7998746B2 (en) * 2000-08-24 2011-08-16 Robert Otillar Systems and methods for localizing and analyzing samples on a bio-sensor chip
KR100384924B1 (ko) * 2000-10-12 2003-05-22 이석근 순환성 자기장을 이용한 유전자 발현 조절장치 및 그 방법
KR100401389B1 (ko) * 2000-10-27 2003-10-17 김희태 핵산 검출 방법, 이를 위한 키트 및 이 키트를 구동시키기위한 구동장치
KR20020032755A (ko) * 2000-10-27 2002-05-04 김희태 핵산 증폭 산물-프로브간의 혼성화반응 효율을 증대시킨핵산 검출 방법
US20030003464A1 (en) * 2000-11-27 2003-01-02 Phan Brigitte C. Dual bead assays including optical biodiscs and methods relating thereto
US20040248093A1 (en) * 2000-11-27 2004-12-09 Coombs James Howard Magneto-optical bio-discs and systems including related methods
US20020172980A1 (en) * 2000-11-27 2002-11-21 Phan Brigitte Chau Methods for decreasing non-specific binding of beads in dual bead assays including related optical biodiscs and disc drive systems
US7079468B2 (en) 2000-12-08 2006-07-18 Burstein Technologies, Inc. Optical discs for measuring analytes
US7054258B2 (en) 2000-12-08 2006-05-30 Nagaoka & Co., Ltd. Optical disc assemblies for performing assays
EP1493014A2 (en) 2001-04-11 2005-01-05 Burstein Technologies, Inc. Multi-parameter assays including analysis discs and methods relating thereto
EP1506413B1 (en) 2002-05-17 2016-07-06 Becton Dickinson and Company Automated system for isolating, amplyifying and detecting a target nucleic acid sequence
CN1230531C (zh) 2002-12-09 2005-12-07 清华大学 从样品中分离细胞粒子的方法
CN1223680C (zh) * 2002-12-10 2005-10-19 清华大学 扩增靶细胞或病毒的核酸的方法
TWI342395B (en) 2002-12-20 2011-05-21 Ibm Method for producing a monolayer of molecules on a surface and biosensor with such a monolayer
US7601491B2 (en) 2003-02-06 2009-10-13 Becton, Dickinson And Company Pretreatment method for extraction of nucleic acid from biological samples and kits therefor
JP4501430B2 (ja) * 2004-01-08 2010-07-14 カシオ計算機株式会社 検査装置、検査方法及び合成装置
WO2013012276A2 (ko) * 2011-07-19 2013-01-24 주식회사 진바이오시스 수소자기반응 유전자조절기 및 유전자조절 방법
ITTO20120302A1 (it) 2012-04-05 2013-10-06 St Microelectronics Srl Dispositivo integrato e metodo per la reazione a catena della polimerasi quantitativa in tempo reale
US9044729B2 (en) 2012-07-27 2015-06-02 International Park Of Creativity Methods and devices for electromagnetic amplification of nucleic acids
AU2013202805B2 (en) 2013-03-14 2015-07-16 Gen-Probe Incorporated System and method for extending the capabilities of a diagnostic analyzer
US10011841B2 (en) 2013-08-02 2018-07-03 International Park Of Creativity Methods and devices for electromagnetic ligation of nucleic acids and electromagnetic transformation of cells
CN107349885A (zh) * 2017-08-28 2017-11-17 华南理工大学 一种固体碱催化反应器
CN108465550B (zh) * 2018-02-12 2020-09-15 徐州德坤电气科技有限公司 一种连续运动介质的杂质或异物分离装置
CN108480039B (zh) * 2018-02-12 2020-09-15 徐州德坤电气科技有限公司 一种连续运动介质的杂质或异物分离装置控制方法

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NO168811C (no) * 1989-05-31 1992-04-08 Dynal As Separasjonsanordning for separering av magnetiserbare partikler
GB8927744D0 (en) * 1989-12-07 1990-02-07 Diatec A S Process and apparatus
BR9206705A (pt) * 1991-11-01 1995-11-21 Adelaide Children S Hospital Processo de amplificação de fase sólida
GB9201481D0 (en) * 1992-01-23 1992-03-11 Scient Generics Ltd Treatment of nucleic acid material
US5401632A (en) * 1992-07-16 1995-03-28 Wisconsin Alumni Research Foundation Triple helix purification and sequencing
AU690124B2 (en) * 1993-06-09 1998-04-23 Gamera Bioscience Corporation Magnetic cycle reaction

Also Published As

Publication number Publication date
EP0796345A2 (en) 1997-09-24
JPH10510158A (ja) 1998-10-06
WO1996017959A3 (en) 1996-08-29
DK0796345T3 (da) 1999-05-25
ATE178098T1 (de) 1999-04-15
DE69508617T2 (de) 1999-07-29
DE69508617D1 (de) 1999-04-29
AU691076B2 (en) 1998-05-07
CA2207388A1 (en) 1996-06-13
CN1175282A (zh) 1998-03-04
AU4517596A (en) 1996-06-26
WO1996017959A2 (en) 1996-06-13
EP0796345B1 (en) 1999-03-24
US5686271A (en) 1997-11-11

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