ES2131279T3 - Inmunoensayo de tipo sandwich para n-peptido. - Google Patents

Inmunoensayo de tipo sandwich para n-peptido.

Info

Publication number
ES2131279T3
ES2131279T3 ES95308892T ES95308892T ES2131279T3 ES 2131279 T3 ES2131279 T3 ES 2131279T3 ES 95308892 T ES95308892 T ES 95308892T ES 95308892 T ES95308892 T ES 95308892T ES 2131279 T3 ES2131279 T3 ES 2131279T3
Authority
ES
Spain
Prior art keywords
peptide
monoclonal antibody
mixture
recognizing
sandwich type
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
ES95308892T
Other languages
English (en)
Inventor
Yoshito Numata
Hidehisa Asada
Keiji Dohi
Takahiro Fukunaga
Yasushi Taniguchi
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Shionogi and Co Ltd
Original Assignee
Shionogi and Co Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Shionogi and Co Ltd filed Critical Shionogi and Co Ltd
Application granted granted Critical
Publication of ES2131279T3 publication Critical patent/ES2131279T3/es
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6893—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids related to diseases not provided for elsewhere
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C07K16/26—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from animals or humans against hormones ; against hormone releasing or inhibiting factors
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/435—Assays involving biological materials from specific organisms or of a specific nature from animals; from humans
    • G01N2333/575—Hormones
    • G01N2333/58—Atrial natriuretic factor complex; Atriopeptin; Atrial natriuretic peptide [ANP]; Brain natriuretic peptide [BNP, proBNP]; Cardionatrin; Cardiodilatin
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2474/00—Immunochemical assays or immunoassays characterised by detection mode or means of detection
    • G01N2474/20—Immunohistochemistry assay
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S436/00—Chemistry: analytical and immunological testing
    • Y10S436/807—Apparatus included in process claim, e.g. physical support structures
    • Y10S436/808—Automated or kit
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S436/00—Chemistry: analytical and immunological testing
    • Y10S436/811—Test for named disease, body condition or organ function
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S530/00—Chemistry: natural resins or derivatives; peptides or proteins; lignins or reaction products thereof
    • Y10S530/808—Materials and products related to genetic engineering or hybrid or fused cell technology, e.g. hybridoma, monoclonal products
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S530/00—Chemistry: natural resins or derivatives; peptides or proteins; lignins or reaction products thereof
    • Y10S530/808—Materials and products related to genetic engineering or hybrid or fused cell technology, e.g. hybridoma, monoclonal products
    • Y10S530/809—Fused cells, e.g. hybridoma
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S530/00—Chemistry: natural resins or derivatives; peptides or proteins; lignins or reaction products thereof
    • Y10S530/827—Proteins from mammals or birds
    • Y10S530/829—Blood

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Immunology (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Biomedical Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Organic Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biotechnology (AREA)
  • Food Science & Technology (AREA)
  • Microbiology (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Analytical Chemistry (AREA)
  • Cell Biology (AREA)
  • Genetics & Genomics (AREA)
  • Biophysics (AREA)
  • Endocrinology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)

Abstract

LA PRESENTE INVENCION SE REFIERE METODOS ALTERNATIVOS PARA LLEVAR A CABO UN INMUNOENSAYO PARA LA DETERMINACION RAPIDA Y FACIL DE UN N-PEPTIDO MEDIANTE LA UTILIZACION DE DOS TIPOS DE ANTICUERPOS MONOCLONALES CAPACES DE RECONOCER DIFERENTES PORCIONES DEL N-PEPTIDO. EL METODO PARA DETERMINAR EL N-PEPTIDO O UN PRECURSOR DEL MISMO CONSISTE EN LOS PASOS SIGUIENTES: SE INCUBA UNA MEZCLA QUE CONTENGA UNA MUESTRA Y UN PRIMER ANTICUERPO MONOCLONAL MARCADO QUE RECONOZCA UNA PORCION DEL N-PEPTIDO; SE AÑADE A LA MEZCLA UN SEGUNDO ANTICUERPO MONOCLONAL QUE RECONOZCA UNA PORCION DEL N-PEPTIDO, TRAS LO QUE SE SIGUE CON LA INCUBACION; Y SE DETECTA EL COMPLEJO DE ANTIGENO Y ANTICUERPO RESULTANTE EN LA MEZCLA. ALTERNATIVAMENTE, EL METODO CONSISTE EN LOS PASOS SIGUIENTES: SE INCUBA UNA MEZCLA QUE CONTENGA UNA MUESTRA, UN PRIMER ANTICUERPO MONOCLONAL QUE RECONOZCA UNA PORCION DEL N-PEPTIDO Y UN SEGUNDO ANTICUERPO MONOCLONAL MARCADO QUE RECONOZCA OTRA PORCION DEL N-PEPTIDO; Y SE DETECTA EL COMPLEJO DE ANTIGENO YANTICUERPO RESULTANTE.
ES95308892T 1994-12-09 1995-12-07 Inmunoensayo de tipo sandwich para n-peptido. Expired - Lifetime ES2131279T3 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
JP30645394 1994-12-09

Publications (1)

Publication Number Publication Date
ES2131279T3 true ES2131279T3 (es) 1999-07-16

Family

ID=17957191

Family Applications (1)

Application Number Title Priority Date Filing Date
ES95308892T Expired - Lifetime ES2131279T3 (es) 1994-12-09 1995-12-07 Inmunoensayo de tipo sandwich para n-peptido.

Country Status (8)

Country Link
US (3) US5702910A (es)
EP (1) EP0721105B1 (es)
KR (1) KR100375564B1 (es)
AT (1) ATE180058T1 (es)
DE (1) DE69509626T2 (es)
ES (1) ES2131279T3 (es)
FI (1) FI119990B (es)
NO (1) NO319913B1 (es)

Families Citing this family (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
BR0316560A (pt) * 2002-11-26 2005-10-04 Nobex Corp Conjugado de composto natriurético, formulação farmacêutica, método de tratar uma condição, método de preparação do conjugado de composto natriurético, conjugado de peptìdeo pró-polinatriurético modificado, análogo de peptìdeo natriurético, análogo de hbnp, composto, e, método de preparar um composto
US7648962B2 (en) * 2002-11-26 2010-01-19 Biocon Limited Natriuretic compounds, conjugates, and uses thereof
DE10316583A1 (de) * 2003-04-10 2004-10-28 B.R.A.H.M.S Aktiengesellschaft Bestimmung eines midregionalen Proadrenomedullin-Teilpeptids in biologischen Flüssigkeiten zu diagnostischen Zwecken, sowie Immunoassays für die Durchführung einer solchen Bestimmung
WO2006076471A2 (en) * 2005-01-12 2006-07-20 Nobex Corporation Bnp conjugates and methods of use
DE102007010834A1 (de) 2007-03-03 2008-09-04 Brahms Aktiengesellschaft Diagnose und Risikostratifizierung von Herzinsuffizienz für NYHA I Patienten
DE102007022367A1 (de) 2007-05-07 2008-11-20 B.R.A.H.M.S Ag Verfahren zur Bestimmung von aminoterminalen pro-ANP bei übergewichtigen Patienten
EP2180322A1 (en) * 2008-10-22 2010-04-28 BRAHMS Aktiengesellschaft Prognostic biomarkers for the progression of primary chronic kidney disease
JP2014210761A (ja) * 2013-04-05 2014-11-13 株式会社シバヤギ 抗イヌｎ末端プロ心房性ナトリウム利尿ペプチド抗体ならびにそれを用いた免疫学的測定方法、および免疫学的測定用キット

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4618600A (en) * 1984-04-19 1986-10-21 Biotechnology Research Associates, J.V. Novel polypeptide diuretic/vasodilators
JPH01216997A (ja) * 1988-02-24 1989-08-30 Fujisawa Pharmaceut Co Ltd 新規セフェム化合物ならびにその製造法
JP2724315B2 (ja) * 1988-02-29 1998-03-09 塩野義製薬株式会社 α−ＡＮＰを認識するモノクローナル抗体およびα−ＡＮＰの免疫学的測定法
GB8815796D0 (en) * 1988-07-02 1988-08-10 Bendix Ltd Gas compressing system
JP2561513B2 (ja) * 1988-07-04 1996-12-11 塩野義製薬株式会社 γ−ＡＮＰを認識するモノクローナル抗体

Also Published As

Publication number Publication date
US5859203A (en) 1999-01-12
US5702910A (en) 1997-12-30
NO954979D0 (no) 1995-12-07
US5827674A (en) 1998-10-27
KR960024367A (ko) 1996-07-20
EP0721105A1 (en) 1996-07-10
NO319913B1 (no) 2005-10-03
FI955894L (fi) 1996-06-10
FI955894A0 (fi) 1995-12-08
EP0721105B1 (en) 1999-05-12
ATE180058T1 (de) 1999-05-15
KR100375564B1 (ko) 2003-05-12
NO954979L (no) 1996-06-10
DE69509626T2 (de) 2000-01-13
FI119990B (fi) 2009-05-29
DE69509626D1 (de) 1999-06-17

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