ES2134206T3 - Metodo para detectar visualmente la presencia de un virus en muestras clinicas. - Google Patents

Metodo para detectar visualmente la presencia de un virus en muestras clinicas.

Info

Publication number
ES2134206T3
ES2134206T3 ES92902876T ES92902876T ES2134206T3 ES 2134206 T3 ES2134206 T3 ES 2134206T3 ES 92902876 T ES92902876 T ES 92902876T ES 92902876 T ES92902876 T ES 92902876T ES 2134206 T3 ES2134206 T3 ES 2134206T3
Authority
ES
Spain
Prior art keywords
virus
clinical samples
enzyme
chromogen
precipitate
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
ES92902876T
Other languages
English (en)
Inventor
Avraham Liav
James F Maher
Craig D Shimasaki
C Worth Clinkscales
Michael D Roark
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Oklahoma Medical Research Foundation
Original Assignee
Oklahoma Medical Research Foundation
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Oklahoma Medical Research Foundation filed Critical Oklahoma Medical Research Foundation
Application granted granted Critical
Publication of ES2134206T3 publication Critical patent/ES2134206T3/es
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
    • C12Q1/04—Determining presence or kind of microorganism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2334/00—O-linked chromogens for determinations of hydrolase enzymes, e.g. glycosidases, phosphatases, esterases
    • C12Q2334/30—Naphthol derivatives, e.g. alpha-naphthyl-esters, i.e. alpha-NE, beta-naphthyl-esters, i.e. beta-NE
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2334/00—O-linked chromogens for determinations of hydrolase enzymes, e.g. glycosidases, phosphatases, esterases
    • C12Q2334/70—O-linked chromogens for determinations of hydrolase enzymes, e.g. glycosidases, phosphatases, esterases the product, e.g. phenol, naphthol being diazotised in situ, e.g. with Fast Red

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Analytical Chemistry (AREA)
  • Toxicology (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

UN ENSAYO RAPIDO Y DIRECTO PARA DETECTAR UN VIRUS QUE TIENE UN ENZIMA CARACTERISTICO EN LA MUESTRA CLINICA.LA MUESTRA ES PUESTA EN CONTACTO CON UNA SOLUCION QUE TIENE UN SUSTRATO PARA EL ENZIMA Y QUE INCLUYE UN CROMOGEN OBTENIDO DEL SUSTRATO POR EL ENZIMA.UN AGENTE PRECIPITADOR REACCIONA CON EL CROMOGEN LIBERADO PARA FORMAR UN PRECIPITADO,LA SOLUCION SE FILTRA PARA CONCENTRAR EL PRECIPITADO Y SE PUEDE OBSERVAR EL PRECIPITADO CONCENTRADO POR EL COLOR CARACTERISTICO DEL CROMOGEN.
ES92902876T 1990-12-31 1991-12-30 Metodo para detectar visualmente la presencia de un virus en muestras clinicas. Expired - Lifetime ES2134206T3 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US63584990A 1990-12-31 1990-12-31

Publications (1)

Publication Number Publication Date
ES2134206T3 true ES2134206T3 (es) 1999-10-01

Family

ID=24549372

Family Applications (1)

Application Number Title Priority Date Filing Date
ES92902876T Expired - Lifetime ES2134206T3 (es) 1990-12-31 1991-12-30 Metodo para detectar visualmente la presencia de un virus en muestras clinicas.

Country Status (9)

Country Link
EP (1) EP0566633B1 (es)
AT (1) ATE182925T1 (es)
AU (1) AU656162B2 (es)
CA (1) CA2104683C (es)
DE (1) DE69131504T2 (es)
DK (1) DK0566633T3 (es)
ES (1) ES2134206T3 (es)
GR (1) GR3030926T3 (es)
WO (1) WO1992012256A1 (es)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
IL125662A0 (en) 1996-03-01 1999-04-11 Biota Scient Management Method of detecting influenza virus and compounds for use therein
US6512100B1 (en) 1997-10-27 2003-01-28 Ibbex, Inc. Chromogenic substrates of sialidase and methods of making and using the same
US6667161B1 (en) 1997-10-27 2003-12-23 Ibbex, Inc. Chromogenic substrates of sialidase of bacterial, viral, protozoa, and vertebrate origin and methods of making and using the same
JP2003522113A (ja) * 1998-10-27 2003-07-22 ユーエービー リサーチ ファンデイション シアリダーゼの発色基質とその製造法および使用法
DE10028837A1 (de) 2000-06-15 2001-12-20 Roche Diagnostics Gmbh Verfahren zum Nachweis von Influenza A/B-Viren

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3950322A (en) * 1973-08-27 1976-04-13 Research Corporation Fluorogenic substrate glycosides
JPS5925699A (ja) * 1982-08-03 1984-02-09 Torii Yakuhin Kk 酵素活性測定方法
US4810636A (en) * 1986-12-09 1989-03-07 Miles Inc. Chromogenic acridinone enzyme substrates
DK0507854T3 (da) * 1989-12-29 1997-05-12 Oklahoma Med Res Found Fremgangsmåder til diagnosticering af human-influenza og chromogene N-acetylneuraminsyresubstrater, der er modificerede i 4-positionen, til anvendelse deri

Also Published As

Publication number Publication date
ATE182925T1 (de) 1999-08-15
CA2104683A1 (en) 1992-07-01
EP0566633A1 (en) 1993-10-27
DE69131504D1 (de) 1999-09-09
EP0566633A4 (en) 1994-12-14
DK0566633T3 (da) 2000-03-06
AU9152991A (en) 1992-08-17
DE69131504T2 (de) 1999-11-18
GR3030926T3 (en) 1999-11-30
AU656162B2 (en) 1995-01-27
CA2104683C (en) 2004-06-29
WO1992012256A1 (en) 1992-07-23
EP0566633B1 (en) 1999-08-04

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