ES2137468T3 - Oligonucleotido utilizable como iniciador en un procedimiento de amplificacion basado en una replica con desplazamiento de cadena. - Google Patents

Oligonucleotido utilizable como iniciador en un procedimiento de amplificacion basado en una replica con desplazamiento de cadena.

Info

Publication number
ES2137468T3
ES2137468T3 ES95402411T ES95402411T ES2137468T3 ES 2137468 T3 ES2137468 T3 ES 2137468T3 ES 95402411 T ES95402411 T ES 95402411T ES 95402411 T ES95402411 T ES 95402411T ES 2137468 T3 ES2137468 T3 ES 2137468T3
Authority
ES
Spain
Prior art keywords
replica
initiator
procedure based
amplification procedure
chain displacement
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
ES95402411T
Other languages
English (en)
Inventor
Philippe Cleuziat
Francoise Guillou-Bonnici
Pierre Levasseur
Francois Mallet
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Biomerieux SA
Original Assignee
Biomerieux SA
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Biomerieux SA filed Critical Biomerieux SA
Application granted granted Critical
Publication of ES2137468T3 publication Critical patent/ES2137468T3/es
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6853—Nucleic acid amplification reactions using modified primers or templates

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)

Abstract

LA INVENCION SE REFIERE A OLIGONUCLEOTIDOS COMPRENDIENDO, DESDE 5'' HACIA 3'', UNA PRIMERA PARTE QUE DISPONE DE UN SEGMENTO ENTRE ELLOS Y UN SEGMENTO QUE FORMA UNA ESTRUCTURA DE TRONCO DE BUCLE, CON UNA SEGUNDA PARTE DE AUTOAPAREAMIENTO NO SUSCEPTIBLE. LA PRIMERA PARTE COMPRENDE AL MENOS UN AGENTE DE BLOQUEO, CON CAPACIDAD DE BLOQUEAR LA REPLICA MEDIANTE UNA POLIMERASA DEL OLIGONUCLEOTIDO, DE FORMA QUE AL MENOS UNA PARTE DEL SEGMENTO SEA REPLICADA Y DE MANERA QUE EL SEGMENTO INTERMEDIO NO SEA REPLICADO NI TOTAL Y PARCIALMENTE. ESTE OLIGONUCLEOTIDO ES UTILIZADO COMO CEBO EN UN PROCESO DE AMPLIFICACION CICLICA DE UNA SECUENCIA CICLICA DE UN ACIDO NUCLEICO.
ES95402411T 1994-10-28 1995-10-27 Oligonucleotido utilizable como iniciador en un procedimiento de amplificacion basado en una replica con desplazamiento de cadena. Expired - Lifetime ES2137468T3 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
FR9413010A FR2726277B1 (fr) 1994-10-28 1994-10-28 Oligonucleotide utilisable comme amorce dans une methode d'amplification basee sur une replication avec deplacement de brin

Publications (1)

Publication Number Publication Date
ES2137468T3 true ES2137468T3 (es) 1999-12-16

Family

ID=9468373

Family Applications (1)

Application Number Title Priority Date Filing Date
ES95402411T Expired - Lifetime ES2137468T3 (es) 1994-10-28 1995-10-27 Oligonucleotido utilizable como iniciador en un procedimiento de amplificacion basado en una replica con desplazamiento de cadena.

Country Status (8)

Country Link
US (1) US5874260A (es)
EP (1) EP0713922B1 (es)
AT (1) ATE184324T1 (es)
CA (1) CA2161577C (es)
DE (1) DE69512003T2 (es)
DK (1) DK0713922T3 (es)
ES (1) ES2137468T3 (es)
FR (1) FR2726277B1 (es)

Families Citing this family (59)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6261808B1 (en) * 1992-08-04 2001-07-17 Replicon, Inc. Amplification of nucleic acid molecules via circular replicons
WO1994003624A1 (en) * 1992-08-04 1994-02-17 Auerbach Jeffrey I Methods for the isothermal amplification of nucleic acid molecules
US5854033A (en) 1995-11-21 1998-12-29 Yale University Rolling circle replication reporter systems
US8445664B2 (en) 1998-06-24 2013-05-21 Enzo Diagnostics, Inc. Kits for amplifying and detecting nucleic acid sequences
US6743605B1 (en) * 1998-06-24 2004-06-01 Enzo Life Sciences, Inc. Linear amplification of specific nucleic acid sequences
US6410278B1 (en) 1998-11-09 2002-06-25 Eiken Kagaku Kabushiki Kaisha Process for synthesizing nucleic acid
JP3974441B2 (ja) * 1998-11-09 2007-09-12 栄研化学株式会社 核酸の合成方法
GB9917816D0 (en) * 1999-07-29 1999-09-29 Cytocell Ltd Chemically modified probes used to regulate nucleic acid amplification assays
JP4721603B2 (ja) * 1999-11-08 2011-07-13 栄研化学株式会社 変異および／または多型の検出方法
KR100612551B1 (ko) * 1999-11-08 2006-08-11 에이켄 카가꾸 가부시끼가이샤 핵산의 합성방법
WO2001034838A1 (en) * 1999-11-08 2001-05-17 Eiken Kagaku Kabushiki Kaisha Method of detecting variation or polymorphism
EP2253717B1 (en) * 2000-04-07 2014-04-30 Eiken Kagaku Kabushiki Kaisha Method of amplifying nucleic acid by using double-stranded nucleic acid as template
US20060166227A1 (en) * 2000-06-20 2006-07-27 Stephen Kingsmore Protein expression profiling
US6323009B1 (en) * 2000-06-28 2001-11-27 Molecular Staging, Inc. Multiply-primed amplification of nucleic acid sequences
US6573051B2 (en) 2001-03-09 2003-06-03 Molecular Staging, Inc. Open circle probes with intramolecular stem structures
US8137911B2 (en) 2001-05-22 2012-03-20 Cellscript, Inc. Preparation and use of single-stranded transcription substrates for synthesis of transcription products corresponding to target sequences
US7553619B2 (en) * 2002-02-08 2009-06-30 Qiagen Gmbh Detection method using dissociated rolling circle amplification
WO2004022784A2 (en) * 2002-09-06 2004-03-18 Johns Hopkins Singapore Pte Ltd Strand specific detection and quantification
US7413857B2 (en) * 2002-11-21 2008-08-19 Epicentre Technologies Methods for using riboprimers for strand displacement replication of target sequences
US20060094016A1 (en) * 2002-12-02 2006-05-04 Niall Gormley Determination of methylation of nucleic acid sequences
AU2003295085A1 (en) * 2002-12-02 2004-06-23 Solexa Limited Recovery of original template
US9487823B2 (en) * 2002-12-20 2016-11-08 Qiagen Gmbh Nucleic acid amplification
US6977153B2 (en) 2002-12-31 2005-12-20 Qiagen Gmbh Rolling circle amplification of RNA
US8043834B2 (en) * 2003-03-31 2011-10-25 Qiagen Gmbh Universal reagents for rolling circle amplification and methods of use
US20040248103A1 (en) * 2003-06-04 2004-12-09 Feaver William John Proximity-mediated rolling circle amplification
US7314714B2 (en) * 2003-12-19 2008-01-01 Affymetrix, Inc. Method of oligonucleotide synthesis
US7575863B2 (en) 2004-05-28 2009-08-18 Applied Biosystems, Llc Methods, compositions, and kits comprising linker probes for quantifying polynucleotides
EP1791982B1 (en) * 2004-09-21 2011-01-05 Life Technologies Corporation TWO-COLOR REAL-TIME/END-POINT QUANTITATION OF MICRORNAS (MIRNAs)
US8309303B2 (en) * 2005-04-01 2012-11-13 Qiagen Gmbh Reverse transcription and amplification of RNA with simultaneous degradation of DNA
US20070026430A1 (en) * 2005-06-30 2007-02-01 Applera Corporation Proximity probing of target proteins comprising restriction and/or extension
ATE510930T1 (de) * 2005-08-02 2011-06-15 Rubicon Genomics Inc Zusammensetzungen und verfahren zur bearbeitung und amplifikation von dna mit verwendung mehrerer enzyme in einer einzigen reaktion
EP1762627A1 (de) 2005-09-09 2007-03-14 Qiagen GmbH Verfahren zur Aktivierung einer Nukleinsäure für eine Polymerase-Reaktion
DE102006020885A1 (de) * 2006-05-05 2007-11-08 Qiagen Gmbh Einführung von Sequenzelementen in Nukleinsäuren
US7833716B2 (en) 2006-06-06 2010-11-16 Gen-Probe Incorporated Tagged oligonucleotides and their use in nucleic acid amplification methods
JP2008029333A (ja) * 2006-07-03 2008-02-14 Fujifilm Corp 新規遺伝子増幅法に用いられるプライマー
EP2484781B1 (en) 2006-08-01 2017-08-23 Applied Biosystems, LLC Detection of analytes and nucleic acids
WO2008096146A1 (en) 2007-02-07 2008-08-14 Solexa Limited Preparation of templates for methylation analysis
US20090215057A1 (en) * 2007-11-19 2009-08-27 Reimo Tetzner Analysis molecule and method for the analysis of a nucleotide position in a nucleic acid
US8405379B1 (en) * 2008-09-18 2013-03-26 Luc Montagnier System and method for the analysis of DNA sequences in biological fluids
ES2743029T3 (es) 2008-10-24 2020-02-18 Epicentre Tech Corporation Composiciones de extremo del transposón y métodos para modificar ácidos nucleicos
US9080211B2 (en) 2008-10-24 2015-07-14 Epicentre Technologies Corporation Transposon end compositions and methods for modifying nucleic acids
JP5785942B2 (ja) * 2009-06-29 2015-09-30 ルミネックス コーポレーション ヘアピンコンフォメーションを有するキメラプライマーおよびその使用法
MX357803B (es) * 2012-03-27 2018-07-24 Curevac Ag Moléculas de ácido nucleico artificiales.
AU2014233145A1 (en) 2013-03-15 2015-09-17 Theranos Ip Company, Llc Nucleic acid amplification
CA2903874C (en) 2013-03-15 2024-02-20 Theranos, Inc. Nucleic acid amplification
US10450595B2 (en) 2013-03-15 2019-10-22 Theranos Ip Company, Llc Nucleic acid amplification
JP2016521120A (ja) 2013-03-15 2016-07-21 セラノス， インコーポレイテッド 核酸増幅
US10155977B2 (en) * 2013-08-09 2018-12-18 Genebio Systems, Inc. DNA amplification via scissor-like structures (DASL)
WO2015035260A1 (en) 2013-09-06 2015-03-12 Theranos, Inc. Systems and methods for detecting infectious diseases
US10017759B2 (en) 2014-06-26 2018-07-10 Illumina, Inc. Library preparation of tagged nucleic acid
CN107109495B (zh) 2014-11-11 2021-12-24 伊鲁米那股份有限公司 使用crispr-cas系统的多核苷酸扩增
EP3455372B1 (en) 2016-05-11 2019-10-30 Illumina, Inc. Polynucleotide enrichment and amplification using argonaute systems
EP3565907B1 (en) 2017-01-06 2022-05-04 Editas Medicine, Inc. Methods of assessing nuclease cleavage
US10914729B2 (en) 2017-05-22 2021-02-09 The Trustees Of Princeton University Methods for detecting protein binding sequences and tagging nucleic acids
WO2019166530A1 (en) 2018-03-02 2019-09-06 F. Hoffmann-La Roche Ag Generation of single-stranded circular dna templates for single molecule sequencing
CN110129311B (zh) * 2019-04-02 2023-04-11 沈阳航空航天大学 一种无立足点和分支迁移域的dna链置换新方法
KR20230154078A (ko) 2021-03-09 2023-11-07 일루미나, 인코포레이티드 CAS-gRNA 리보핵단백질을 사용한 게놈 라이브러리 제작 및 표적화된 후성적 검정
BR112023018157A2 (pt) 2021-03-09 2023-10-31 Illumina Inc Análise de expressão de variantes codificantes de proteína em células
ES3035187T3 (en) 2021-08-11 2025-08-29 Illumina Inc Detection of analytes using targeted epigenetic assays, proximity-induced tagmentation, strand invasion, restriction, or ligation

Family Cites Families (28)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4683195A (en) 1986-01-30 1987-07-28 Cetus Corporation Process for amplifying, detecting, and/or-cloning nucleic acid sequences
US4683202A (en) 1985-03-28 1987-07-28 Cetus Corporation Process for amplifying nucleic acid sequences
US4800159A (en) 1986-02-07 1989-01-24 Cetus Corporation Process for amplifying, detecting, and/or cloning nucleic acid sequences
FR2607507B1 (fr) 1986-12-02 1990-04-13 Centre Nat Rech Scient Nouveaux derives a-d-oligonucleotides, leur preparation et leur emploi
IL86724A (en) 1987-06-19 1995-01-24 Siska Diagnostics Inc Methods and kits for amplification and testing of nucleic acid sequences
AU622426B2 (en) 1987-12-11 1992-04-09 Abbott Laboratories Assay using template-dependent nucleic acid probe reorganization
CA1340807C (en) 1988-02-24 1999-11-02 Lawrence T. Malek Nucleic acid amplification process
US5130238A (en) 1988-06-24 1992-07-14 Cangene Corporation Enhanced nucleic acid amplification process
WO1990001069A1 (en) 1988-07-20 1990-02-08 Segev Diagnostics, Inc. Process for amplifying and detecting nucleic acid sequences
WO1990006995A1 (en) 1988-12-16 1990-06-28 Siska Diagnostics, Inc. Self-sustained sequence replication system
GB8903567D0 (en) * 1989-02-16 1989-04-05 British Telecomm An optical network
US5043272A (en) * 1989-04-27 1991-08-27 Life Technologies, Incorporated Amplification of nucleic acid sequences using oligonucleotides of random sequence as primers
US5040193A (en) * 1989-07-28 1991-08-13 At&T Bell Laboratories Receiver and digital phase-locked loop for burst mode data recovery
US5215899A (en) * 1989-11-09 1993-06-01 Miles Inc. Nucleic acid amplification employing ligatable hairpin probe and transcription
US5103466A (en) * 1990-03-26 1992-04-07 Intel Corporation CMOS digital clock and data recovery circuit
DE4010465A1 (de) * 1990-03-31 1991-10-02 Boehringer Mannheim Gmbh Verfahren zur spezifischen vervielfaeltigung von nukleinsaeuretemplates und zur bestimmung von nukleinsaeuren
US5194370A (en) * 1990-05-16 1993-03-16 Life Technologies, Inc. Promoter ligation activated transcription amplification of nucleic acid sequences
FR2663040B1 (fr) 1990-06-11 1995-09-15 Bio Merieux Procede de detection d'une sequence nucleotidique selon la technique d'hybridation sandwich.
AU8997991A (en) 1991-01-31 1992-08-06 Becton Dickinson & Company Exonuclease mediated strand displacement amplification
FR2679255B1 (fr) 1991-07-17 1993-10-22 Bio Merieux Procede d'immobilisation d'un fragment nucleique par fixation passive sur un support solide, support solide ainsi obtenu et son utilisation.
CA2073630C (en) * 1991-08-30 2007-12-11 Atsushi Ohshima Method for synthesizing single-stranded stem-loop dnas, the products and uses therefor
EP0648281A4 (en) * 1991-09-10 1997-06-04 Jack D Love TARGETED AMPLIFICATION OF DNA AND RNA AND SIGNAL AMPLIFICATION.
DE4213029A1 (de) * 1991-09-26 1993-04-01 Boehringer Mannheim Gmbh Verfahren zur spezifischen vervielfaeltigung von nukleins aeuresequenzen
DE4132133A1 (de) * 1991-09-26 1993-04-01 Boehringer Mannheim Gmbh Verfahren zur spezifischen herstellung von ribonukleinsaeuren
US5411875A (en) * 1991-11-01 1995-05-02 University Of Iowa Research Foundation Method for retrieval of unknown flanking DNA sequence
US5270184A (en) * 1991-11-19 1993-12-14 Becton, Dickinson And Company Nucleic acid target generation
WO1993017127A1 (en) 1992-02-20 1993-09-02 The State Of Oregon Acting By And Through The Oregon State Board Of Higher Education On Behalf Of Oregon State University Boomerand dna amplification
US5400370A (en) * 1993-02-24 1995-03-21 Advanced Micro Devices Inc. All digital high speed algorithmic data recovery method and apparatus using locally generated compensated broad band time rulers and data edge position averaging

Also Published As

Publication number Publication date
DE69512003D1 (de) 1999-10-14
DK0713922T3 (da) 2000-03-27
FR2726277A1 (fr) 1996-05-03
ATE184324T1 (de) 1999-09-15
EP0713922B1 (fr) 1999-09-08
DE69512003T2 (de) 2000-05-25
FR2726277B1 (fr) 1996-12-27
CA2161577C (fr) 2010-08-24
EP0713922A1 (fr) 1996-05-29
CA2161577A1 (fr) 1996-04-29
US5874260A (en) 1999-02-23

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