ES2140209T3 - Procedimiento y dispositivo de cromatografia de liquidos de alto rendimiento. - Google Patents

Procedimiento y dispositivo de cromatografia de liquidos de alto rendimiento.

Info

Publication number
ES2140209T3
ES2140209T3 ES97904852T ES97904852T ES2140209T3 ES 2140209 T3 ES2140209 T3 ES 2140209T3 ES 97904852 T ES97904852 T ES 97904852T ES 97904852 T ES97904852 T ES 97904852T ES 2140209 T3 ES2140209 T3 ES 2140209T3
Authority
ES
Spain
Prior art keywords
column
fluids
eluant
injections
entrance
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
ES97904852T
Other languages
English (en)
Inventor
Hubert M Quinn
Rebecca A Menapace
Charles J Oberhauser
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Cohesive Technologies Inc
Original Assignee
Cohesive Technologies Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Cohesive Technologies Inc filed Critical Cohesive Technologies Inc
Application granted granted Critical
Publication of ES2140209T3 publication Critical patent/ES2140209T3/es
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
    • G01N30/26—Conditioning of the fluid carrier; Flow patterns
    • G01N30/28—Control of physical parameters of the fluid carrier
    • G01N30/32—Control of physical parameters of the fluid carrier of pressure or speed
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/10—Selective adsorption, e.g. chromatography characterised by constructional or operational features
    • B01D15/16—Selective adsorption, e.g. chromatography characterised by constructional or operational features relating to the conditioning of the fluid carrier
    • B01D15/163—Pressure or speed conditioning
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
    • G01N30/04—Preparation or injection of sample to be analysed
    • G01N30/16—Injection
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/10—Selective adsorption, e.g. chromatography characterised by constructional or operational features
    • B01D15/20—Selective adsorption, e.g. chromatography characterised by constructional or operational features relating to the conditioning of the sorbent material
    • B01D15/203—Equilibration or regeneration
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/42—Selective adsorption, e.g. chromatography characterised by the development mode, e.g. by displacement or by elution
    • B01D15/424—Elution mode
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
    • G01N30/26—Conditioning of the fluid carrier; Flow patterns
    • G01N30/28—Control of physical parameters of the fluid carrier
    • G01N30/32—Control of physical parameters of the fluid carrier of pressure or speed
    • G01N2030/324—Control of physical parameters of the fluid carrier of pressure or speed speed, flow rate
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
    • G01N30/26—Conditioning of the fluid carrier; Flow patterns
    • G01N30/38—Flow patterns
    • G01N2030/387—Turbulent flow of mobile phase

Landscapes

  • Chemical & Material Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Treatment Of Liquids With Adsorbents In General (AREA)

Abstract

APARATO DE CROMATOGRAFIA LIQUIDA DE ALTA PRESION EN CUYA COLUMNA SE CARGA UNA MEZCLA DE FLUIDOS QUE CONTIENE COMO MINIMO UN SOLUTO QUE ES REACTIVO CON LAS SUPERFICIES CROMATOGRAFICAMENTE REACTIVAS DE LA COLUMNA, Y SE INYECTAN UN NUMERO DE VOLUMENES DE DIFERENTES FLUIDOS ELUYENTES EN ESA COLUMNA. LAS INYECCIONES SE REALIZAN DE FORMA QUE SE MINIMICE LA CANTIDAD REQUERIDA DE ELUYENTE. LAS INYECCIONES CORRESPONDIENTES A UNA OPERACION SE EFECTUAN DE FORMA QUE SE ASEGURE QUE, COMO MINIMO, SE PRODUZCA EL FLUJO DE LOS LIQUIDOS ELUYENTES POR LA COLUMNA, PREFERIBLEMENTE CON UN FRENTE DE ONDA PRACTICAMENTE PLANO, A UNAS VELOCIDADES CORRESPONDIENTES A UNAS VELOCIDADES REDUCIDAS SUPERIORES A 5.000, POR EJEMPLO, Y UNOS FLUJOS SUFICIENTES PARA PROVOCAR UN FLUJO TURBULENTO EN AQUELLOS FLUIDOS, LO QUE MINIMIZA EL TIEMPO REQUERIDO PARA QUE PASE TODA LA MEZCLA Y LOS FLUIDOS ELUYENTES POR LA COLUMNA. EN OTRAS OPERACIONES, LAS INYECCIONES SE REALIZAN PRACTICAMENTE SIMULTANEAS EN PUNTOS ESPACIALMENTE SEPARADOS ADYACENTES A LA ENTRADA DE LA COLUMNA O EN SECUENCIA EN UNA UNICA LOCALIZACION ADYACENTE A LA ENTRADA DE LA COLUMNA, PERO EN TODOS LOS CASOS LOS FLUIDOS TIENDEN A VIAJAR POR LA COLUMNA A UNA VELOCIDAD DE GRUPO COMUN, COMO SI SE TRATARA DE CILINDROS DE FLUIDOS ESTRECHAMENTE AGRUPADOS.
ES97904852T 1996-01-19 1997-01-21 Procedimiento y dispositivo de cromatografia de liquidos de alto rendimiento. Expired - Lifetime ES2140209T3 (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US58887496A 1996-01-19 1996-01-19
US2721696P 1996-09-30 1996-09-30

Publications (1)

Publication Number Publication Date
ES2140209T3 true ES2140209T3 (es) 2000-02-16

Family

ID=26702215

Family Applications (1)

Application Number Title Priority Date Filing Date
ES97904852T Expired - Lifetime ES2140209T3 (es) 1996-01-19 1997-01-21 Procedimiento y dispositivo de cromatografia de liquidos de alto rendimiento.

Country Status (12)

Country Link
US (2) US5795469A (es)
EP (1) EP0840893B1 (es)
JP (1) JP3452936B2 (es)
AT (1) ATE184702T1 (es)
AU (1) AU712735B2 (es)
CA (1) CA2242885C (es)
DE (1) DE69700523T2 (es)
DK (1) DK0840893T3 (es)
ES (1) ES2140209T3 (es)
NZ (1) NZ330867A (es)
PL (1) PL327920A1 (es)
WO (1) WO1997026532A1 (es)

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Also Published As

Publication number Publication date
AU1753797A (en) 1997-08-11
JP3452936B2 (ja) 2003-10-06
US5795469A (en) 1998-08-18
CA2242885A1 (en) 1997-07-24
JP2000504414A (ja) 2000-04-11
US5968367A (en) 1999-10-19
NZ330867A (en) 1998-11-25
WO1997026532A1 (en) 1997-07-24
AU712735B2 (en) 1999-11-11
CA2242885C (en) 2006-08-15
ATE184702T1 (de) 1999-10-15
DE69700523T2 (de) 2000-04-27
PL327920A1 (en) 1999-01-04
EP0840893B1 (en) 1999-09-15
EP0840893A1 (en) 1998-05-13
DE69700523D1 (de) 1999-10-21
DK0840893T3 (da) 2000-04-03

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