ES2147176T3 - Dispositivo para la aplicacion de muestras biologicas sobre una placa de electroforesis. - Google Patents
Dispositivo para la aplicacion de muestras biologicas sobre una placa de electroforesis.Info
- Publication number
- ES2147176T3 ES2147176T3 ES91403377T ES91403377T ES2147176T3 ES 2147176 T3 ES2147176 T3 ES 2147176T3 ES 91403377 T ES91403377 T ES 91403377T ES 91403377 T ES91403377 T ES 91403377T ES 2147176 T3 ES2147176 T3 ES 2147176T3
- Authority
- ES
- Spain
- Prior art keywords
- lands
- porous membrane
- sample
- membrane
- land
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
- 238000001962 electrophoresis Methods 0.000 title abstract 8
- 239000012472 biological sample Substances 0.000 title abstract 7
- 239000012528 membrane Substances 0.000 abstract 18
- 239000000523 sample Substances 0.000 abstract 8
- 239000000463 material Substances 0.000 abstract 6
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 abstract 4
- 239000012530 fluid Substances 0.000 abstract 4
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 abstract 3
- 239000011148 porous material Substances 0.000 abstract 3
- 239000003351 stiffener Substances 0.000 abstract 3
- 239000000080 wetting agent Substances 0.000 abstract 3
- PUPZLCDOIYMWBV-UHFFFAOYSA-N (+/-)-1,3-Butanediol Chemical compound CC(O)CCO PUPZLCDOIYMWBV-UHFFFAOYSA-N 0.000 abstract 2
- 229920002678 cellulose Polymers 0.000 abstract 2
- 229920002301 cellulose acetate Polymers 0.000 abstract 2
- 238000001514 detection method Methods 0.000 abstract 2
- 230000008020 evaporation Effects 0.000 abstract 2
- 238000001704 evaporation Methods 0.000 abstract 2
- 235000011187 glycerol Nutrition 0.000 abstract 2
- 238000009736 wetting Methods 0.000 abstract 2
- DQEFEBPAPFSJLV-UHFFFAOYSA-N Cellulose propionate Chemical compound CCC(=O)OCC1OC(OC(=O)CC)C(OC(=O)CC)C(OC(=O)CC)C1OC1C(OC(=O)CC)C(OC(=O)CC)C(OC(=O)CC)C(COC(=O)CC)O1 DQEFEBPAPFSJLV-UHFFFAOYSA-N 0.000 abstract 1
- 239000000020 Nitrocellulose Substances 0.000 abstract 1
- 229910019142 PO4 Inorganic materials 0.000 abstract 1
- 239000013504 Triton X-100 Substances 0.000 abstract 1
- 229920004890 Triton X-100 Polymers 0.000 abstract 1
- FJWGYAHXMCUOOM-QHOUIDNNSA-N [(2s,3r,4s,5r,6r)-2-[(2r,3r,4s,5r,6s)-4,5-dinitrooxy-2-(nitrooxymethyl)-6-[(2r,3r,4s,5r,6s)-4,5,6-trinitrooxy-2-(nitrooxymethyl)oxan-3-yl]oxyoxan-3-yl]oxy-3,5-dinitrooxy-6-(nitrooxymethyl)oxan-4-yl] nitrate Chemical compound O([C@@H]1O[C@@H]([C@H]([C@H](O[N+]([O-])=O)[C@H]1O[N+]([O-])=O)O[C@H]1[C@@H]([C@@H](O[N+]([O-])=O)[C@H](O[N+]([O-])=O)[C@@H](CO[N+]([O-])=O)O1)O[N+]([O-])=O)CO[N+](=O)[O-])[C@@H]1[C@@H](CO[N+]([O-])=O)O[C@@H](O[N+]([O-])=O)[C@H](O[N+]([O-])=O)[C@H]1O[N+]([O-])=O FJWGYAHXMCUOOM-QHOUIDNNSA-N 0.000 abstract 1
- 239000002253 acid Substances 0.000 abstract 1
- 239000001913 cellulose Substances 0.000 abstract 1
- 229920006218 cellulose propionate Polymers 0.000 abstract 1
- 239000003795 chemical substances by application Substances 0.000 abstract 1
- 230000002209 hydrophobic effect Effects 0.000 abstract 1
- 239000007788 liquid Substances 0.000 abstract 1
- 229920001220 nitrocellulos Polymers 0.000 abstract 1
- NBIIXXVUZAFLBC-UHFFFAOYSA-K phosphate Chemical compound [O-]P([O-])([O-])=O NBIIXXVUZAFLBC-UHFFFAOYSA-K 0.000 abstract 1
- 239000010452 phosphate Substances 0.000 abstract 1
- 229920000136 polysorbate Polymers 0.000 abstract 1
- 239000004627 regenerated cellulose Substances 0.000 abstract 1
- 239000011780 sodium chloride Substances 0.000 abstract 1
- 239000004094 surface-active agent Substances 0.000 abstract 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
- G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
- G01N27/416—Systems
- G01N27/447—Systems using electrophoresis
- G01N27/44704—Details; Accessories
- G01N27/44743—Introducing samples
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D57/00—Separation, other than separation of solids, not fully covered by a single other group or subclass, e.g. B03C
- B01D57/02—Separation, other than separation of solids, not fully covered by a single other group or subclass, e.g. B03C by electrophoresis
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Molecular Biology (AREA)
- Electrochemistry (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Physics & Mathematics (AREA)
- Environmental & Geological Engineering (AREA)
- Engineering & Computer Science (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Sampling And Sample Adjustment (AREA)
- Peptides Or Proteins (AREA)
- Manufacture Of Porous Articles, And Recovery And Treatment Of Waste Products (AREA)
Abstract
LA INVENCION SE REFIERE A UN DISPOSITIVO PARA LA APLICACION DE MUESTRAS BIOLOGICAS, EN PARTICULAR SOBRE UN GEL DE ELECTROFORESIS QUE COMPRENDE: ATERIAL POROSO SOBRE LOS QUE UNA MUESTRA BIOLOGICA ES SUSCEPTIBLE DE SER DEPOSITADA, SIENDO ESTOS ELEMENTOS PLANOS EN PARTICULAR LLEVADOS POR EL BORDE DE UNA MEMBRANA PLANA POROSA, SOLIDARIOS DE LA MEMBRANA POROSA Y EN LA PROLONGACION DEL PLANO DE ESTA, Y EN VOLADIZO RESPECTO DE DICHA MEMBRANA POROSA, NDOSE A CONTINUACION ESTOS ELEMENTOS "ELEMENTOS EN VOLADIZO", COMPRENDIENDO LA PARTE NO INTRODUCIDA EN LA MEMBRANA POROSA DE LOS ELEMENTOS EN VOLADIZO UN EXTREMO LIBRE, SIENDO TALES DICHOS ELEMENTOS EN VOLADIZO QUE TIENEN TODOS AL MENOS UN PUNTO SUSCEPTIBLE DE SER PUESTO EN CONTACTO CON LA SUPERFICIE PLANA DE LA PLACA DE ELECTROFORESIS PARA DEPOSITAR SOBRE DICHA PLACA UNA O VARIAS MUESTRAS BIOLOGICAS, SIENDO CADA MUESTRA BIOLOGICA PREVIAMENTE DEPOSITADA SOBRE UN ELEMENTO DE LOS ELEMENTOS EN VOLADIZO, SIENDO ESTOS DIFERENTES PUNTOS SUSCEPTIBLES DE SER PUESTOS SIMULTANEAMENTE EN CONTACTO CON LA SUPERFICIE DE DICHA PLACA SEGUN UNA ALINEACION, CUANDO DICHA MEMBRANA POROSA, O DICHOS MEDIOS DE RIGIDIFICACION, SE DEPOSITAN EN UN PLANO INCLINADO O PERPENDICULAR RESPECTO DE LA SUPERFICIE DE DICHA PLACA.
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| FR9100092A FR2671290B1 (fr) | 1991-01-04 | 1991-01-04 | Dispositif pour l'application d'echantillons biologiques sur une plaque d'electrophorese. |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| ES2147176T3 true ES2147176T3 (es) | 2000-09-01 |
Family
ID=9408472
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| ES91403377T Expired - Lifetime ES2147176T3 (es) | 1991-01-04 | 1991-12-12 | Dispositivo para la aplicacion de muestras biologicas sobre una placa de electroforesis. |
Country Status (10)
| Country | Link |
|---|---|
| US (1) | US5464515A (es) |
| EP (2) | EP0493996B1 (es) |
| JP (2) | JP3124808B2 (es) |
| AT (2) | ATE191365T1 (es) |
| DE (2) | DE69133456D1 (es) |
| DK (1) | DK0493996T3 (es) |
| ES (1) | ES2147176T3 (es) |
| FR (1) | FR2671290B1 (es) |
| GR (1) | GR3033802T3 (es) |
| SG (1) | SG44795A1 (es) |
Families Citing this family (34)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| FR2715229B1 (fr) * | 1994-01-14 | 1996-05-10 | Sebia Sa | Dispositif pour l'immunofixation sur un support (plaqué) unique, d'échantillons différents. |
| US5683915A (en) * | 1995-10-31 | 1997-11-04 | Isolab, Inc. | Sample deposition device and method |
| US5681437A (en) * | 1995-10-31 | 1997-10-28 | Isolab, Inc. | Sample deposition device |
| EP1197750B1 (en) * | 1996-05-06 | 2008-07-02 | Helena Laboratories Corporation | A system for the application of samples on a substrate |
| DE19700626A1 (de) * | 1997-01-10 | 1998-07-16 | Europ Lab Molekularbiolog | Probenzuführung |
| GB9718320D0 (en) * | 1997-09-01 | 1997-11-05 | Medical Res Council | Improvements in or relating to gel loading |
| US5939022A (en) * | 1997-12-09 | 1999-08-17 | Pharmacia Biotech, Inc. | Article for transporting biological samples during analysis |
| DE19830989C1 (de) * | 1998-07-10 | 2000-04-13 | Lion Bioscience Ag | Verwendung von porösen Membranmaterialien als Beladungsmaterialien bei der Gelelektrophorese |
| FR2805175B1 (fr) | 2000-02-22 | 2002-05-10 | Sebia Sa | Compositions utilisables pour l'hydratation d'un support d'electrophorese, pour l'amelioration de l'electrophorese de zone |
| FR2806161B1 (fr) | 2000-03-10 | 2002-09-20 | Sebia Sa | Methode de separation, d'identification et de quantification des isophosphatases alcalines par electrophorese |
| FR2809818B1 (fr) * | 2000-05-31 | 2003-10-24 | Sebia Sa | Identification positive d'echantillons analyses par electrophorese sur support poreux |
| US6582577B1 (en) | 2000-08-31 | 2003-06-24 | Visible Genetics Inc. | Electrophoresis gel cassette |
| WO2002055189A2 (en) * | 2001-01-12 | 2002-07-18 | Syngenta Participations Ag | Nanoporous membrane reactor for miniaturized reactions and enhanced reaction kinetics |
| JP4601460B2 (ja) * | 2005-03-02 | 2010-12-22 | 株式会社常光 | 簡易塗布器 |
| JP4601461B2 (ja) * | 2005-03-02 | 2010-12-22 | 株式会社常光 | 電気泳動装置における検体塗布機構 |
| EP1979410B1 (en) * | 2005-12-29 | 2012-08-22 | Life Technologies Corporation | Compositions and methods for improving resolution of biomolecules separated on polyacrylamide gels |
| US8562804B2 (en) | 2006-07-20 | 2013-10-22 | The Board Of Trustees Of The Leland Stanford Junior University | Fluorescent finger prints for indirect detection in isotachophoresis |
| US8865401B2 (en) | 2007-12-14 | 2014-10-21 | The Johns Hopkins University | Purification and concentration of proteins and DNA from a complex sample using isotachophoresis and a device to perform the purification |
| WO2009079028A1 (en) * | 2007-12-14 | 2009-06-25 | Johns Hopkins University | Purification and concentration of proteins and dna from a complex sample using isotachophoresis and a device to perform the purification |
| EP2277036A1 (en) * | 2008-04-14 | 2011-01-26 | Phorexy | Apparatus and method for non immersed gel electrophoresis |
| JP4957917B2 (ja) * | 2008-07-15 | 2012-06-20 | 凸版印刷株式会社 | 電気泳動器具および電気泳動方法 |
| US8846314B2 (en) * | 2009-03-03 | 2014-09-30 | The Board Of Trustees Of The Leland Stanford Junior University | Isotachophoretic focusing of nucleic acids |
| US8449745B2 (en) | 2009-06-26 | 2013-05-28 | Yi Wang | Monolithic electrophoresis flat gel system |
| US8361293B2 (en) | 2009-06-26 | 2013-01-29 | Yi Wang | Monolithic electrophoresis gel system |
| US8361294B2 (en) | 2009-06-26 | 2013-01-29 | Yi Wang | Monolithic electrophoresis gel system |
| US8721858B2 (en) * | 2010-03-12 | 2014-05-13 | The Board Of Trustees Of The Leland Stanford Junior University | Non-focusing tracers for indirect detection in electrophoretic displacement techniques |
| US8986529B2 (en) | 2010-09-13 | 2015-03-24 | The Board Of Trustees Of The Leland Stanford Junior University | Isotachophoresis having interacting anionic and cationic shock waves |
| US8524061B2 (en) | 2010-11-29 | 2013-09-03 | The Board Of Trustees Of The Leland Stanford Junior University | On-chip hybridization coupled with ITP based purification for fast sequence specific identification |
| US20150160156A1 (en) * | 2012-06-04 | 2015-06-11 | Sharp Kabushiki Kaisha | Cassette for electrophoresis and electrophoresis method |
| WO2015144618A1 (en) * | 2014-03-28 | 2015-10-01 | Ge Healthcare Bio-Sciences Ab | Electrophoresis separation method |
| EP3916090B1 (en) | 2016-01-29 | 2025-07-23 | Purigen Biosystems, Inc. | Isotachophoresis for purification of nucleic acids |
| EP3655768B1 (en) * | 2017-07-21 | 2026-04-29 | Helena Laboratories Corporation | Method for depositing antisera in immunofixation electrophoresis |
| SG11202000871WA (en) | 2017-08-02 | 2020-02-27 | Purigen Biosystems Inc | Systems, devices, and methods for isotachophoresis |
| JP2022525842A (ja) | 2019-02-11 | 2022-05-20 | アジレント・テクノロジーズ・インク | 処理前の流体サンプルの平面状への予整形 |
Family Cites Families (18)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US3317418A (en) * | 1963-02-28 | 1967-05-02 | Beckman Instruments Inc | Electrophoresis apparatus with adjustable sample application |
| US3616387A (en) * | 1969-11-13 | 1971-10-26 | Bio Rad Laboratories | Method and apparatus for the transfer of fluid samples |
| US3930973A (en) * | 1972-03-10 | 1976-01-06 | Nerenberg Samuel T | Electrophoretic process |
| US3855846A (en) * | 1972-04-07 | 1974-12-24 | Marine Colloids Inc | Sample applicator |
| GB1448581A (en) * | 1973-01-23 | 1976-09-08 | Shandon Southern Instr Ltd | Applicators |
| US3839183A (en) * | 1973-06-15 | 1974-10-01 | Beckman Instruments Inc | Electrophoresis sample applicator |
| JPS5343437B2 (es) * | 1974-06-27 | 1978-11-20 | ||
| US3928203A (en) * | 1975-03-27 | 1975-12-23 | Schleicher & Schuell Inc | Chromatographic apparatus |
| US4004548A (en) * | 1975-07-02 | 1977-01-25 | Eastman Kodak Company | Chromatographic spotter |
| US4086372A (en) * | 1975-10-08 | 1978-04-25 | Helena Laboratories Corporation | Method for applying an organic liquid sample |
| DE2614192C2 (de) * | 1976-04-02 | 1986-09-18 | FMC Corp., Wilmington, Del. | Analysenvorrichtung und Analysenverfahren |
| JPS5739789Y2 (es) * | 1977-12-15 | 1982-09-01 | ||
| JPS55161238U (es) * | 1979-05-04 | 1980-11-19 | ||
| US4272381A (en) * | 1979-10-22 | 1981-06-09 | Schleicher & Schuell, Inc. | Thin-layer chromatography spotting |
| US4351800A (en) * | 1981-02-06 | 1982-09-28 | Biochemical Diagnostics, Inc. | Thin layer plate chromatography apparatus |
| US4696187A (en) * | 1981-02-06 | 1987-09-29 | Biochemical Diagnostics, Inc. | Chromatography method |
| US4812241A (en) * | 1987-08-14 | 1989-03-14 | Laser Precision Corporation | Sample transfer for infrared analysis in thin layer chromatography-structure & method |
| JPH0178945U (es) * | 1987-11-17 | 1989-05-26 |
-
1991
- 1991-01-04 FR FR9100092A patent/FR2671290B1/fr not_active Expired - Lifetime
- 1991-12-12 DK DK91403377T patent/DK0493996T3/da active
- 1991-12-12 EP EP91403377A patent/EP0493996B1/fr not_active Expired - Lifetime
- 1991-12-12 DE DE69133456T patent/DE69133456D1/de not_active Expired - Lifetime
- 1991-12-12 AT AT91403377T patent/ATE191365T1/de not_active IP Right Cessation
- 1991-12-12 ES ES91403377T patent/ES2147176T3/es not_active Expired - Lifetime
- 1991-12-12 AT AT99100556T patent/ATE294957T1/de not_active IP Right Cessation
- 1991-12-12 SG SG1996007719A patent/SG44795A1/en unknown
- 1991-12-12 DE DE69132095T patent/DE69132095T2/de not_active Expired - Lifetime
- 1991-12-12 EP EP99100556A patent/EP0911631B1/fr not_active Expired - Lifetime
- 1991-12-27 JP JP03358129A patent/JP3124808B2/ja not_active Expired - Lifetime
-
1993
- 1993-06-04 US US08/072,866 patent/US5464515A/en not_active Expired - Lifetime
-
2000
- 2000-06-28 GR GR20000401501T patent/GR3033802T3/el unknown
- 2000-08-30 JP JP2000260123A patent/JP3215100B2/ja not_active Expired - Lifetime
Also Published As
| Publication number | Publication date |
|---|---|
| EP0493996B1 (fr) | 2000-04-05 |
| ATE191365T1 (de) | 2000-04-15 |
| EP0911631B1 (fr) | 2005-05-04 |
| JPH0587765A (ja) | 1993-04-06 |
| DE69133456D1 (de) | 2005-06-09 |
| JP3215100B2 (ja) | 2001-10-02 |
| HK1014680A1 (en) | 1999-09-30 |
| EP0493996A1 (fr) | 1992-07-08 |
| GR3033802T3 (en) | 2000-10-31 |
| FR2671290B1 (fr) | 1993-04-16 |
| SG44795A1 (en) | 1997-12-19 |
| US5464515A (en) | 1995-11-07 |
| ATE294957T1 (de) | 2005-05-15 |
| FR2671290A1 (fr) | 1992-07-10 |
| JP2001091498A (ja) | 2001-04-06 |
| DK0493996T3 (da) | 2000-09-04 |
| DE69132095T2 (de) | 2000-09-14 |
| DE69132095D1 (de) | 2000-05-11 |
| EP0911631A1 (fr) | 1999-04-28 |
| JP3124808B2 (ja) | 2001-01-15 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| FG2A | Definitive protection |
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