GR3033802T3 - Apparatus for applying of biological samples to an electrophoresis plate. - Google Patents
Apparatus for applying of biological samples to an electrophoresis plate.Info
- Publication number
- GR3033802T3 GR3033802T3 GR20000401501T GR20000401501T GR3033802T3 GR 3033802 T3 GR3033802 T3 GR 3033802T3 GR 20000401501 T GR20000401501 T GR 20000401501T GR 20000401501 T GR20000401501 T GR 20000401501T GR 3033802 T3 GR3033802 T3 GR 3033802T3
- Authority
- GR
- Greece
- Prior art keywords
- lands
- porous membrane
- sample
- membrane
- land
- Prior art date
Links
- 238000001962 electrophoresis Methods 0.000 title abstract 8
- 239000012472 biological sample Substances 0.000 title abstract 7
- 239000012528 membrane Substances 0.000 abstract 18
- 239000000523 sample Substances 0.000 abstract 8
- 239000000463 material Substances 0.000 abstract 6
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 abstract 4
- 239000012530 fluid Substances 0.000 abstract 4
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 abstract 3
- 239000011148 porous material Substances 0.000 abstract 3
- 239000003351 stiffener Substances 0.000 abstract 3
- 239000000080 wetting agent Substances 0.000 abstract 3
- PUPZLCDOIYMWBV-UHFFFAOYSA-N (+/-)-1,3-Butanediol Chemical compound CC(O)CCO PUPZLCDOIYMWBV-UHFFFAOYSA-N 0.000 abstract 2
- 229920002678 cellulose Polymers 0.000 abstract 2
- 229920002301 cellulose acetate Polymers 0.000 abstract 2
- 238000001514 detection method Methods 0.000 abstract 2
- 230000008020 evaporation Effects 0.000 abstract 2
- 238000001704 evaporation Methods 0.000 abstract 2
- 235000011187 glycerol Nutrition 0.000 abstract 2
- 238000009736 wetting Methods 0.000 abstract 2
- DQEFEBPAPFSJLV-UHFFFAOYSA-N Cellulose propionate Chemical compound CCC(=O)OCC1OC(OC(=O)CC)C(OC(=O)CC)C(OC(=O)CC)C1OC1C(OC(=O)CC)C(OC(=O)CC)C(OC(=O)CC)C(COC(=O)CC)O1 DQEFEBPAPFSJLV-UHFFFAOYSA-N 0.000 abstract 1
- 239000000020 Nitrocellulose Substances 0.000 abstract 1
- 229910019142 PO4 Inorganic materials 0.000 abstract 1
- 239000013504 Triton X-100 Substances 0.000 abstract 1
- 229920004890 Triton X-100 Polymers 0.000 abstract 1
- FJWGYAHXMCUOOM-QHOUIDNNSA-N [(2s,3r,4s,5r,6r)-2-[(2r,3r,4s,5r,6s)-4,5-dinitrooxy-2-(nitrooxymethyl)-6-[(2r,3r,4s,5r,6s)-4,5,6-trinitrooxy-2-(nitrooxymethyl)oxan-3-yl]oxyoxan-3-yl]oxy-3,5-dinitrooxy-6-(nitrooxymethyl)oxan-4-yl] nitrate Chemical compound O([C@@H]1O[C@@H]([C@H]([C@H](O[N+]([O-])=O)[C@H]1O[N+]([O-])=O)O[C@H]1[C@@H]([C@@H](O[N+]([O-])=O)[C@H](O[N+]([O-])=O)[C@@H](CO[N+]([O-])=O)O1)O[N+]([O-])=O)CO[N+](=O)[O-])[C@@H]1[C@@H](CO[N+]([O-])=O)O[C@@H](O[N+]([O-])=O)[C@H](O[N+]([O-])=O)[C@H]1O[N+]([O-])=O FJWGYAHXMCUOOM-QHOUIDNNSA-N 0.000 abstract 1
- 239000002253 acid Substances 0.000 abstract 1
- 239000001913 cellulose Substances 0.000 abstract 1
- 229920006218 cellulose propionate Polymers 0.000 abstract 1
- 239000003795 chemical substances by application Substances 0.000 abstract 1
- 230000002209 hydrophobic effect Effects 0.000 abstract 1
- 239000007788 liquid Substances 0.000 abstract 1
- 229920001220 nitrocellulos Polymers 0.000 abstract 1
- NBIIXXVUZAFLBC-UHFFFAOYSA-K phosphate Chemical compound [O-]P([O-])([O-])=O NBIIXXVUZAFLBC-UHFFFAOYSA-K 0.000 abstract 1
- 239000010452 phosphate Substances 0.000 abstract 1
- 229920000136 polysorbate Polymers 0.000 abstract 1
- 239000004627 regenerated cellulose Substances 0.000 abstract 1
- 239000011780 sodium chloride Substances 0.000 abstract 1
- 239000004094 surface-active agent Substances 0.000 abstract 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
- G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
- G01N27/416—Systems
- G01N27/447—Systems using electrophoresis
- G01N27/44704—Details; Accessories
- G01N27/44743—Introducing samples
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D57/00—Separation, other than separation of solids, not fully covered by a single other group or subclass, e.g. B03C
- B01D57/02—Separation, other than separation of solids, not fully covered by a single other group or subclass, e.g. B03C by electrophoresis
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Molecular Biology (AREA)
- Electrochemistry (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Physics & Mathematics (AREA)
- Environmental & Geological Engineering (AREA)
- Engineering & Computer Science (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Sampling And Sample Adjustment (AREA)
- Peptides Or Proteins (AREA)
- Manufacture Of Porous Articles, And Recovery And Treatment Of Waste Products (AREA)
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| FR9100092A FR2671290B1 (fr) | 1991-01-04 | 1991-01-04 | Dispositif pour l'application d'echantillons biologiques sur une plaque d'electrophorese. |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| GR3033802T3 true GR3033802T3 (en) | 2000-10-31 |
Family
ID=9408472
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| GR20000401501T GR3033802T3 (en) | 1991-01-04 | 2000-06-28 | Apparatus for applying of biological samples to an electrophoresis plate. |
Country Status (10)
| Country | Link |
|---|---|
| US (1) | US5464515A (el) |
| EP (2) | EP0493996B1 (el) |
| JP (2) | JP3124808B2 (el) |
| AT (2) | ATE191365T1 (el) |
| DE (2) | DE69133456D1 (el) |
| DK (1) | DK0493996T3 (el) |
| ES (1) | ES2147176T3 (el) |
| FR (1) | FR2671290B1 (el) |
| GR (1) | GR3033802T3 (el) |
| SG (1) | SG44795A1 (el) |
Families Citing this family (34)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| FR2715229B1 (fr) * | 1994-01-14 | 1996-05-10 | Sebia Sa | Dispositif pour l'immunofixation sur un support (plaqué) unique, d'échantillons différents. |
| US5683915A (en) * | 1995-10-31 | 1997-11-04 | Isolab, Inc. | Sample deposition device and method |
| US5681437A (en) * | 1995-10-31 | 1997-10-28 | Isolab, Inc. | Sample deposition device |
| EP1197750B1 (en) * | 1996-05-06 | 2008-07-02 | Helena Laboratories Corporation | A system for the application of samples on a substrate |
| DE19700626A1 (de) * | 1997-01-10 | 1998-07-16 | Europ Lab Molekularbiolog | Probenzuführung |
| GB9718320D0 (en) * | 1997-09-01 | 1997-11-05 | Medical Res Council | Improvements in or relating to gel loading |
| US5939022A (en) * | 1997-12-09 | 1999-08-17 | Pharmacia Biotech, Inc. | Article for transporting biological samples during analysis |
| DE19830989C1 (de) * | 1998-07-10 | 2000-04-13 | Lion Bioscience Ag | Verwendung von porösen Membranmaterialien als Beladungsmaterialien bei der Gelelektrophorese |
| FR2805175B1 (fr) | 2000-02-22 | 2002-05-10 | Sebia Sa | Compositions utilisables pour l'hydratation d'un support d'electrophorese, pour l'amelioration de l'electrophorese de zone |
| FR2806161B1 (fr) | 2000-03-10 | 2002-09-20 | Sebia Sa | Methode de separation, d'identification et de quantification des isophosphatases alcalines par electrophorese |
| FR2809818B1 (fr) * | 2000-05-31 | 2003-10-24 | Sebia Sa | Identification positive d'echantillons analyses par electrophorese sur support poreux |
| US6582577B1 (en) | 2000-08-31 | 2003-06-24 | Visible Genetics Inc. | Electrophoresis gel cassette |
| WO2002055189A2 (en) * | 2001-01-12 | 2002-07-18 | Syngenta Participations Ag | Nanoporous membrane reactor for miniaturized reactions and enhanced reaction kinetics |
| JP4601460B2 (ja) * | 2005-03-02 | 2010-12-22 | 株式会社常光 | 簡易塗布器 |
| JP4601461B2 (ja) * | 2005-03-02 | 2010-12-22 | 株式会社常光 | 電気泳動装置における検体塗布機構 |
| EP1979410B1 (en) * | 2005-12-29 | 2012-08-22 | Life Technologies Corporation | Compositions and methods for improving resolution of biomolecules separated on polyacrylamide gels |
| US8562804B2 (en) | 2006-07-20 | 2013-10-22 | The Board Of Trustees Of The Leland Stanford Junior University | Fluorescent finger prints for indirect detection in isotachophoresis |
| US8865401B2 (en) | 2007-12-14 | 2014-10-21 | The Johns Hopkins University | Purification and concentration of proteins and DNA from a complex sample using isotachophoresis and a device to perform the purification |
| WO2009079028A1 (en) * | 2007-12-14 | 2009-06-25 | Johns Hopkins University | Purification and concentration of proteins and dna from a complex sample using isotachophoresis and a device to perform the purification |
| EP2277036A1 (en) * | 2008-04-14 | 2011-01-26 | Phorexy | Apparatus and method for non immersed gel electrophoresis |
| JP4957917B2 (ja) * | 2008-07-15 | 2012-06-20 | 凸版印刷株式会社 | 電気泳動器具および電気泳動方法 |
| US8846314B2 (en) * | 2009-03-03 | 2014-09-30 | The Board Of Trustees Of The Leland Stanford Junior University | Isotachophoretic focusing of nucleic acids |
| US8449745B2 (en) | 2009-06-26 | 2013-05-28 | Yi Wang | Monolithic electrophoresis flat gel system |
| US8361293B2 (en) | 2009-06-26 | 2013-01-29 | Yi Wang | Monolithic electrophoresis gel system |
| US8361294B2 (en) | 2009-06-26 | 2013-01-29 | Yi Wang | Monolithic electrophoresis gel system |
| US8721858B2 (en) * | 2010-03-12 | 2014-05-13 | The Board Of Trustees Of The Leland Stanford Junior University | Non-focusing tracers for indirect detection in electrophoretic displacement techniques |
| US8986529B2 (en) | 2010-09-13 | 2015-03-24 | The Board Of Trustees Of The Leland Stanford Junior University | Isotachophoresis having interacting anionic and cationic shock waves |
| US8524061B2 (en) | 2010-11-29 | 2013-09-03 | The Board Of Trustees Of The Leland Stanford Junior University | On-chip hybridization coupled with ITP based purification for fast sequence specific identification |
| US20150160156A1 (en) * | 2012-06-04 | 2015-06-11 | Sharp Kabushiki Kaisha | Cassette for electrophoresis and electrophoresis method |
| WO2015144618A1 (en) * | 2014-03-28 | 2015-10-01 | Ge Healthcare Bio-Sciences Ab | Electrophoresis separation method |
| EP3916090B1 (en) | 2016-01-29 | 2025-07-23 | Purigen Biosystems, Inc. | Isotachophoresis for purification of nucleic acids |
| EP3655768B1 (en) * | 2017-07-21 | 2026-04-29 | Helena Laboratories Corporation | Method for depositing antisera in immunofixation electrophoresis |
| SG11202000871WA (en) | 2017-08-02 | 2020-02-27 | Purigen Biosystems Inc | Systems, devices, and methods for isotachophoresis |
| JP2022525842A (ja) | 2019-02-11 | 2022-05-20 | アジレント・テクノロジーズ・インク | 処理前の流体サンプルの平面状への予整形 |
Family Cites Families (18)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US3317418A (en) * | 1963-02-28 | 1967-05-02 | Beckman Instruments Inc | Electrophoresis apparatus with adjustable sample application |
| US3616387A (en) * | 1969-11-13 | 1971-10-26 | Bio Rad Laboratories | Method and apparatus for the transfer of fluid samples |
| US3930973A (en) * | 1972-03-10 | 1976-01-06 | Nerenberg Samuel T | Electrophoretic process |
| US3855846A (en) * | 1972-04-07 | 1974-12-24 | Marine Colloids Inc | Sample applicator |
| GB1448581A (en) * | 1973-01-23 | 1976-09-08 | Shandon Southern Instr Ltd | Applicators |
| US3839183A (en) * | 1973-06-15 | 1974-10-01 | Beckman Instruments Inc | Electrophoresis sample applicator |
| JPS5343437B2 (el) * | 1974-06-27 | 1978-11-20 | ||
| US3928203A (en) * | 1975-03-27 | 1975-12-23 | Schleicher & Schuell Inc | Chromatographic apparatus |
| US4004548A (en) * | 1975-07-02 | 1977-01-25 | Eastman Kodak Company | Chromatographic spotter |
| US4086372A (en) * | 1975-10-08 | 1978-04-25 | Helena Laboratories Corporation | Method for applying an organic liquid sample |
| DE2614192C2 (de) * | 1976-04-02 | 1986-09-18 | FMC Corp., Wilmington, Del. | Analysenvorrichtung und Analysenverfahren |
| JPS5739789Y2 (el) * | 1977-12-15 | 1982-09-01 | ||
| JPS55161238U (el) * | 1979-05-04 | 1980-11-19 | ||
| US4272381A (en) * | 1979-10-22 | 1981-06-09 | Schleicher & Schuell, Inc. | Thin-layer chromatography spotting |
| US4351800A (en) * | 1981-02-06 | 1982-09-28 | Biochemical Diagnostics, Inc. | Thin layer plate chromatography apparatus |
| US4696187A (en) * | 1981-02-06 | 1987-09-29 | Biochemical Diagnostics, Inc. | Chromatography method |
| US4812241A (en) * | 1987-08-14 | 1989-03-14 | Laser Precision Corporation | Sample transfer for infrared analysis in thin layer chromatography-structure & method |
| JPH0178945U (el) * | 1987-11-17 | 1989-05-26 |
-
1991
- 1991-01-04 FR FR9100092A patent/FR2671290B1/fr not_active Expired - Lifetime
- 1991-12-12 DK DK91403377T patent/DK0493996T3/da active
- 1991-12-12 EP EP91403377A patent/EP0493996B1/fr not_active Expired - Lifetime
- 1991-12-12 DE DE69133456T patent/DE69133456D1/de not_active Expired - Lifetime
- 1991-12-12 AT AT91403377T patent/ATE191365T1/de not_active IP Right Cessation
- 1991-12-12 ES ES91403377T patent/ES2147176T3/es not_active Expired - Lifetime
- 1991-12-12 AT AT99100556T patent/ATE294957T1/de not_active IP Right Cessation
- 1991-12-12 SG SG1996007719A patent/SG44795A1/en unknown
- 1991-12-12 DE DE69132095T patent/DE69132095T2/de not_active Expired - Lifetime
- 1991-12-12 EP EP99100556A patent/EP0911631B1/fr not_active Expired - Lifetime
- 1991-12-27 JP JP03358129A patent/JP3124808B2/ja not_active Expired - Lifetime
-
1993
- 1993-06-04 US US08/072,866 patent/US5464515A/en not_active Expired - Lifetime
-
2000
- 2000-06-28 GR GR20000401501T patent/GR3033802T3/el unknown
- 2000-08-30 JP JP2000260123A patent/JP3215100B2/ja not_active Expired - Lifetime
Also Published As
| Publication number | Publication date |
|---|---|
| EP0493996B1 (fr) | 2000-04-05 |
| ATE191365T1 (de) | 2000-04-15 |
| EP0911631B1 (fr) | 2005-05-04 |
| JPH0587765A (ja) | 1993-04-06 |
| DE69133456D1 (de) | 2005-06-09 |
| JP3215100B2 (ja) | 2001-10-02 |
| HK1014680A1 (en) | 1999-09-30 |
| EP0493996A1 (fr) | 1992-07-08 |
| FR2671290B1 (fr) | 1993-04-16 |
| SG44795A1 (en) | 1997-12-19 |
| US5464515A (en) | 1995-11-07 |
| ATE294957T1 (de) | 2005-05-15 |
| FR2671290A1 (fr) | 1992-07-10 |
| ES2147176T3 (es) | 2000-09-01 |
| JP2001091498A (ja) | 2001-04-06 |
| DK0493996T3 (da) | 2000-09-04 |
| DE69132095T2 (de) | 2000-09-14 |
| DE69132095D1 (de) | 2000-05-11 |
| EP0911631A1 (fr) | 1999-04-28 |
| JP3124808B2 (ja) | 2001-01-15 |
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