ES2147180T3 - Procedimiento para la deteccion de acidos nucleicos semejantes. - Google Patents

Procedimiento para la deteccion de acidos nucleicos semejantes.

Info

Publication number
ES2147180T3
ES2147180T3 ES92115065T ES92115065T ES2147180T3 ES 2147180 T3 ES2147180 T3 ES 2147180T3 ES 92115065 T ES92115065 T ES 92115065T ES 92115065 T ES92115065 T ES 92115065T ES 2147180 T3 ES2147180 T3 ES 2147180T3
Authority
ES
Spain
Prior art keywords
nucleic acids
procedure
detection
similar nucleic
nucleotide sequences
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
ES92115065T
Other languages
English (en)
Inventor
Stephan Rust
Harald Dr Funke
Gerd Prof Dr Assmann
Rudiger Dr Ruger
Christoph Dr Kessler
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Roche Diagnostics GmbH
Original Assignee
Roche Diagnostics GmbH
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Roche Diagnostics GmbH filed Critical Roche Diagnostics GmbH
Application granted granted Critical
Publication of ES2147180T3 publication Critical patent/ES2147180T3/es
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6827—Hybridisation assays for detection of mutation or polymorphism
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6858—Allele-specific amplification

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)

Abstract

PROCESO PARA LA DETERMINACION DE ACIDOS NUCLEICOS, CUYAS SECUENCIAS NUCLEOTIDAS SE DIFERENCIAN EN UNA POSICION X, PARA LO CUAL SE EMPLEA UN CONJUNTO DE OLIGONUCLEOTIDOS, CUYOS NUCLEOTIDOS CORRESPONDIENTES A LA POSICION X SOLO SON COMPLEMENTARIOS PARA LA POSICION X DE UNO DE LOS ACIDOS NUCLEICOS SIMILARES, SIN EMBARGO, SUS SECUENCIAS NUCLEOTIDAS SE DIFERENCIAN EN RELACION A LA POSICION DE OTRO DESEQUILIBRIO Y, ASI COMO REACTIVOS APROPIADOS PARA LA REALIZACION DEL PROCESO Y CAMPOS DE APLICACION PARA EL PROCESO. CONTRARAMAL RAMAL
ES92115065T 1991-09-06 1992-09-03 Procedimiento para la deteccion de acidos nucleicos semejantes. Expired - Lifetime ES2147180T3 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
DE4129653A DE4129653A1 (de) 1991-09-06 1991-09-06 Verfahren zum nachweis aehnlicher nukleinsaeuren

Publications (1)

Publication Number Publication Date
ES2147180T3 true ES2147180T3 (es) 2000-09-01

Family

ID=6440005

Family Applications (1)

Application Number Title Priority Date Filing Date
ES92115065T Expired - Lifetime ES2147180T3 (es) 1991-09-06 1992-09-03 Procedimiento para la deteccion de acidos nucleicos semejantes.

Country Status (6)

Country Link
US (2) US5605794A (es)
EP (1) EP0530794B1 (es)
JP (1) JPH0813280B2 (es)
AT (1) ATE191748T1 (es)
DE (2) DE4129653A1 (es)
ES (1) ES2147180T3 (es)

Families Citing this family (42)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6100099A (en) 1994-09-06 2000-08-08 Abbott Laboratories Test strip having a diagonal array of capture spots
US5869252A (en) * 1992-03-31 1999-02-09 Abbott Laboratories Method of multiplex ligase chain reaction
US7001722B1 (en) * 1993-06-22 2006-02-21 Baylor College Of Medicine Parallel primer extension approach to nucleic acid sequence analysis
GB9503808D0 (en) * 1995-02-24 1995-04-12 Univ Nottingham Detection assay
US6703228B1 (en) 1998-09-25 2004-03-09 Massachusetts Institute Of Technology Methods and products related to genotyping and DNA analysis
EP1001037A3 (en) * 1998-09-28 2003-10-01 Whitehead Institute For Biomedical Research Pre-selection and isolation of single nucleotide polymorphisms
DE19855469C2 (de) * 1998-12-01 2001-01-11 Michael Esrich Verfahren zur Bestimmung des Apolipoprotein-E-Genotyps in einer menschlichen Probe
DE60045706D1 (de) 1999-03-18 2011-04-21 Exiqon As Detektion von genmutationen mittels lna primer
AU7125401A (en) * 2000-05-19 2001-12-03 David J Marshall Materials and methods for detection of nucleic acids
WO2002004673A1 (en) * 2000-07-12 2002-01-17 Bionex, Inc. Method for detecting sequence variation of nucleic acid
JP2001137000A (ja) * 2000-09-19 2001-05-22 Nobuyuki Hamashima 遺伝子変異の判別方法
DE60137649D1 (de) * 2000-10-14 2009-03-26 Eragen Biosciences Inc Nachweissysteme auf festen trägern und methoden zur verwendung von nicht-standardbasen
CN100385013C (zh) * 2000-10-14 2008-04-30 伊拉根生物科学公司 使用非标准碱基的固相支持体分析系统和方法
WO2002068684A2 (en) * 2001-02-23 2002-09-06 Pyrosequencing Ab Allele-specific primer extension assay
US7407943B2 (en) 2001-08-01 2008-08-05 Isis Pharmaceuticals, Inc. Antisense modulation of apolipoprotein B expression
US7888324B2 (en) 2001-08-01 2011-02-15 Genzyme Corporation Antisense modulation of apolipoprotein B expression
US20050208491A1 (en) * 2002-02-08 2005-09-22 Rudolf Zirwes Specific multiplex analysis of nucleic acids
CA2506654C (en) * 2002-11-07 2014-02-25 Yoichi Matsubara Method of detecting gene mutation
US7511131B2 (en) 2002-11-13 2009-03-31 Genzyme Corporation Antisense modulation of apolipoprotein B expression
US7514212B2 (en) * 2003-01-17 2009-04-07 Eragen Biosciences, Inc. Nucleic acid amplification using non-standard bases
WO2004090153A2 (en) * 2003-04-01 2004-10-21 Eragen Biosciences,Inc. Polymerase inhibitor and method of using same
WO2005081776A2 (en) * 2004-01-30 2005-09-09 Eragen Biosciences, Inc. Materials and methods for the detection of sars
US20050287558A1 (en) 2004-05-05 2005-12-29 Crooke Rosanne M SNPs of apolipoprotein B and modulation of their expression
EP1623996A1 (en) * 2004-08-06 2006-02-08 Deutsches Krebsforschungszentrum Stiftung des öffentlichen Rechts Improved method of selecting a desired protein from a library
WO2006101913A2 (en) * 2005-03-18 2006-09-28 Eragen Biosciences, Inc. Methods for detecting multiple species and subspecies of neiserria
CA2919210C (en) 2005-06-07 2019-03-05 Luminex Corporation Methods for detection and typing of nucleic acids
US7285387B2 (en) * 2005-10-07 2007-10-23 Matsushita Electric Industrial Co., Ltd. Discrimination method of target base in DNA, and allele specific primer used in the method of the same
JP3859688B1 (ja) * 2005-10-07 2006-12-20 松下電器産業株式会社 Ｄｎａが有する標的塩基を判別する方法
JP3859684B1 (ja) * 2005-11-21 2006-12-20 松下電器産業株式会社 Ｄｎａが有する標的塩基を判別する方法
JP3859686B1 (ja) * 2005-11-21 2006-12-20 松下電器産業株式会社 Ｄｎａが有する標的塩基を判別する方法
JP3859687B1 (ja) 2005-11-21 2006-12-20 松下電器産業株式会社 Ｄｎａが有する標的塩基を判別する方法
US20070264694A1 (en) * 2006-04-07 2007-11-15 Eragen Biosciences, Inc. Use of non-standard bases and proximity effects for gene assembly and conversion of non-standard bases to standard bases during dna synthesis
EP2505646A1 (en) 2006-05-05 2012-10-03 Isis Pharmaceuticals, Inc. Compounds and methods for modulating expression of CRP
US9347061B2 (en) 2007-03-24 2016-05-24 Genzyme Corporation Administering antisense oligonucleotides complementary to human apolipoprotein B
US20090270601A1 (en) * 2008-04-21 2009-10-29 Steven Albert Benner Differential detection of single nucleotide polymorphisms
EP2408796B1 (en) 2009-03-16 2020-04-22 Ionis Pharmaceuticals, Inc. Targeting Apolipoprotein B for the reduction of Apolipoprotein C-III
CN102471777B (zh) 2009-07-10 2014-09-24 巴斯夫植物科学有限公司 用于在植物中胚乳-特异性表达的表达盒
CA2778449C (en) * 2009-10-23 2017-06-20 Luminex Corporation Amplification primers with non-standard bases for increased reaction specificity
DE112010005958T5 (de) 2009-12-03 2013-08-14 Basf Plant Science Company Gmbh Expressionskassetten für die Embryo-spezifische Expression in Pflanzen
EP2455485A1 (de) 2010-11-19 2012-05-23 Anagnostics Bioanalysis GmbH Verfahren zum Nachweis von Nukleinsäuren
US11959086B2 (en) 2016-05-20 2024-04-16 BASF Agro B.V. Dual transit peptides for targeting polypeptides
BR112022017941A2 (pt) 2020-03-11 2022-10-18 BASF Agricultural Solutions Seed US LLC Ácido nucleico isolado, gene recombinante, vetor, célula hospedeira, planta, parte de planta ou semente, métodos para expressar um polinucleotídeo, para produzir uma planta, para fornecer atividade pesticida em uma planta e para produzir alimento e uso do ácido nucleico

Family Cites Families (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4683202A (en) * 1985-03-28 1987-07-28 Cetus Corporation Process for amplifying nucleic acid sequences
DK171161B1 (da) * 1985-03-28 1996-07-08 Hoffmann La Roche Fremgangsmåde til påvisning af forekomst eller fravær af mindst én specifik nukleinsyresekvens i en prøve eller til skelnen mellem to forskellige nukleinsyresekvenser i denne prøve
IE72468B1 (en) * 1987-07-31 1997-04-09 Univ Leland Stanford Junior Selective amplification of target polynucleotide sequences
CA1323293C (en) * 1987-12-11 1993-10-19 Keith C. Backman Assay using template-dependent nucleic acid probe reorganization
CA1340807C (en) * 1988-02-24 1999-11-02 Lawrence T. Malek Nucleic acid amplification process
IE61148B1 (en) * 1988-03-10 1994-10-05 Ici Plc Method of detecting nucleotide sequences
ES2056204T3 (es) * 1988-03-18 1994-10-01 Baylor College Medicine Deteccion de las mutaciones mediante oligonucleotidos cebadores competitivos.
AU3539089A (en) * 1988-04-08 1989-11-03 Salk Institute For Biological Studies, The Ligase-based amplification method
AU3694689A (en) * 1988-04-28 1989-11-24 Mark H. Skolnick Amplified sequence polymorphisms (asps)
ATE138106T1 (de) * 1988-07-20 1996-06-15 David Segev Verfahren zur amplifizierung und zum nachweis von nukleinsäuresequenzen
ZA899593B (en) * 1988-12-16 1990-09-26 Siska Diagnostics Inc Self-sustained,sequence replication system
WO1990012115A1 (en) * 1989-03-21 1990-10-18 Collaborative Research, Inc. A dna diagnostic test using an exonuclease activity
GB8918226D0 (en) * 1989-08-09 1989-09-20 Wellcome Found Dna assays

Also Published As

Publication number Publication date
JPH05244996A (ja) 1993-09-24
EP0530794B1 (de) 2000-04-12
ATE191748T1 (de) 2000-04-15
US5605794A (en) 1997-02-25
DE59209826D1 (de) 2000-05-18
EP0530794A1 (de) 1993-03-10
JPH0813280B2 (ja) 1996-02-14
US5728530A (en) 1998-03-17
DE4129653A1 (de) 1993-03-11

Similar Documents

Publication Publication Date Title
DE59209826D1 (de) Verfahren zum Nachweis ähnlicher Nukleinsäuren
CA2172248A1 (en) Self-sustained sequence replication electrochemiluminescent nucleic acid assay
ES2171529T3 (es) Oligonucleotidos y metodos para la deteccion de chlamydia trachomatis.
ATE141956T1 (de) Verfahren zur vervielfältigung von nukleinsäuresequenzen
ES2143680T3 (es) Metodo para caracterizar dna genomico.
ES2183394T3 (es) Analisis de la secuencia de acidos nucleicos.
ATE340866T1 (de) Zusammensetzungen und verfahren für die gleichzeitige detektion und quantifizierung von einer mehrheit spezifischer nuklein säure sequenzen
FI900602A0 (fi) Bestaemning.
ATE238434T1 (de) Eine in kaskaden verlaufende vervielfältigungsreaktion von nukleinsäuren
CA2189941A1 (en) Fluorescence Polarization Detection of Nucleic Acids
DE59608542D1 (de) C-nukleosid-derivate und deren verwendung in der detektion von nukleinsäuren
DE69332666D1 (de) Verfahren zur einfachen nukleotid-primer-verlängerung zum nachweis spezifischer allele und dafür geeigneter kit
AU2038500A (en) Method for the detection of specific nucleic acid sequences by polymerase nucleotide incorporation
FR2709310B1 (fr) Fragment nucléotidique de l'ARN ribosomique 23S de mycobactéries, sondes et amorces dérivées, réactif et procédé de détection.
FI923239A0 (fi) Immobilisering av nukleinsyror.
DE69815370D1 (de) Fluoreszend-konjugaten von nukleosiden und von nukleotiden, ihrer verfahren zur herstellung und ihrer verwendungen
EP0810291A4 (en) PROBE FOR USE IN NUCLEIC ACID ANALYSIS AND METHOD OF DETECTION
SE9500589L (sv) Nukleinsyremolekyl detektion på fasta substrat vid enmolekyl DNA sekvensering
AU7429591A (en) Nucleic acid probes for the detection of giardia lamblia
WO1998035058A3 (en) Improved process for detection and quantification of nucleic acid molecules
FI904657A7 (fi) Nukleiinihappojen toteamismenetelmä, jossa käytetään erisuuruisia alukepitoisuuksia polymeraasiketjureaktiossa
DE3567908D1 (en) Photoimmune detection of dna and rna
WO1997020068A3 (en) Method for generating multiple double stranded nucleic acids

Legal Events

Date Code Title Description
FG2A Definitive protection

Ref document number: 530794

Country of ref document: ES