ES2149901T3 - Procedimiento de obtencion de un edulcorante natural proteico. - Google Patents

Procedimiento de obtencion de un edulcorante natural proteico.

Info

Publication number
ES2149901T3
ES2149901T3 ES95105973T ES95105973T ES2149901T3 ES 2149901 T3 ES2149901 T3 ES 2149901T3 ES 95105973 T ES95105973 T ES 95105973T ES 95105973 T ES95105973 T ES 95105973T ES 2149901 T3 ES2149901 T3 ES 2149901T3
Authority
ES
Spain
Prior art keywords
taumatin
expression
genes
plasmide
fungi
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
ES95105973T
Other languages
English (en)
Inventor
Juan Uriach-Marsal
Victor Rubio-Susan
Cristina Patino-Martin
Kalo-Koenova Eliza Iossif
Catalina Del-Moral-Juarez
Ignacio Faus-Santasusana
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Urquima SA
Original Assignee
Urquima SA
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Urquima SA filed Critical Urquima SA
Application granted granted Critical
Publication of ES2149901T3 publication Critical patent/ES2149901T3/es
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/415—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from plants
    • C07K14/43—Sweetening agents, e.g. thaumatin, monellin
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/02—Fusion polypeptide containing a localisation/targetting motif containing a signal sequence

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Biophysics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • General Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • Zoology (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Mycology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Plant Pathology (AREA)
  • Medicinal Chemistry (AREA)
  • Microbiology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Botany (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Seasonings (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)

Abstract

TAUMATIN II O TAUMATIN I PUEDEN SER OBTENIDOS MEDIANTE LA EXPRESION, NO DE SUS GENES NATURALES, SINO DE LOS SUSTANCIALMENTE OPTIMIZADOS GENES SINTETICOS Y ARTIFICIALES SIGUIENDO REGLAS ESPECIFICAS. PREFERIBLEMENTE LA EXPRESION ES LLEVADA A CABO EN FILAMENTOS DE HONGOS, ESPECIALMENTE HONGOS GRAS Y PARTICULARMENTE LA ESPECIES PENICILLIUM ROQUEFORTII, ASPERGILLUS NIGER Y LA VARIANTE AWAMORI DEL ASPERGILLUS NIGER. PREPARANDO GENES ARTIFICIALES OPTIMIZADOS SUSTANCIALMENTE POR FILAMENTOS DE HONGOS, DESARROLLADOS AQUI POR PRIMERA VEZ EN EL CASO DEL TAUMATIN, PERMITE PARA EXPRESION ALTA DE PROTEINA, HECIENDO EL PROCESO UTIL PARA LA PRODUCCION INDUSTRIAL DE ESTE VALIOSO EDULCORANTE. TAUMATINAS PUEDEN SER OBTENIDAS EXTRACELULARMENTE USANDO UNA PLASMIDA CON UNA PLASMIDA CON UNA SECRECION SEÑAL, Y TAMBIEN INTRACELULARMENTE. EL METODO MAS RECIENTE PUEDE SER USADO EN ALIMENTACION ANIMAL SIN SEPARACION ANTERIOR DEL MICELIO FUNGICO.
ES95105973T 1994-04-21 1995-04-21 Procedimiento de obtencion de un edulcorante natural proteico. Expired - Lifetime ES2149901T3 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
ES09400836A ES2080689B1 (es) 1994-04-21 1994-04-21 Procedimiento de obtencion de un edulcorante natural proteico.

Publications (1)

Publication Number Publication Date
ES2149901T3 true ES2149901T3 (es) 2000-11-16

Family

ID=8285957

Family Applications (2)

Application Number Title Priority Date Filing Date
ES09400836A Expired - Fee Related ES2080689B1 (es) 1994-04-21 1994-04-21 Procedimiento de obtencion de un edulcorante natural proteico.
ES95105973T Expired - Lifetime ES2149901T3 (es) 1994-04-21 1995-04-21 Procedimiento de obtencion de un edulcorante natural proteico.

Family Applications Before (1)

Application Number Title Priority Date Filing Date
ES09400836A Expired - Fee Related ES2080689B1 (es) 1994-04-21 1994-04-21 Procedimiento de obtencion de un edulcorante natural proteico.

Country Status (11)

Country Link
US (1) US5932438A (es)
EP (1) EP0684312B1 (es)
JP (1) JPH0847396A (es)
AT (1) ATE194386T1 (es)
CA (1) CA2147541C (es)
DE (1) DE69517750T2 (es)
DK (1) DK0684312T3 (es)
ES (2) ES2080689B1 (es)
FI (1) FI112794B (es)
GR (1) GR3034500T3 (es)
PT (1) PT684312E (es)

Families Citing this family (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ES2143408B1 (es) * 1998-04-02 2000-12-01 Urquima Sa Promotor y construcciones para expresion de proteinas recombinantes en hongos filamentosos.
AU2002356880A1 (en) 2001-10-31 2003-05-12 Phytex, Llc Phytase-containing animal food and method
AU2003206684A1 (en) * 2002-02-20 2003-09-09 Novozymes A/S Plant polypeptide production
AU2003286128A1 (en) * 2002-12-05 2004-06-23 Novozymes A/S Expression of polypeptides in filamentous fungi
EP1724341A4 (en) * 2004-01-28 2007-08-01 Mitsukan Group Corp NOVEL, TASTE MANAGEMENT MODIFYING PEPTIDE NAS, ITS DNA AND ITS USE
DK4171199T3 (da) * 2020-06-25 2026-04-13 Mycotechnology Inc Sødt protein fra trøffel
JP2024529755A (ja) * 2021-08-20 2024-08-08 ノマド バイオサイエンス ゲゼルシャフト ミット ベシュレンクテル ハフツング タウマチン及び１種又は複数種のレバウジオシドを含む甘味料ブレンド
WO2023021220A1 (en) * 2021-08-20 2023-02-23 Nomad Bioscience Gmbh Sweetening composition comprising thaumatin and mogrosides
JP2025507085A (ja) * 2022-03-09 2025-03-13 フィックス４４ ラブズ プライベート リミテッド 新規タンパク質組成物及び乳製品の配合におけるその使用

Family Cites Families (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE3175357D1 (en) * 1980-12-12 1986-10-23 Unilever Nv Dna sequences encoding various allelic forms of mature thaumatin, recombinant plasmids comprising said dna's and a process for their preparation, bacterial cultures comprising said recombinant plasmids, and method for producing mature thaumatin
DE3177281T2 (de) * 1980-12-12 1992-12-10 Unilever Nv Strukturgene, die fuer die verschiedenen allelischen und reifungsformen von praeprothaumatin und deren mutanten kodieren, diese strukturgene enthaltende klonierungsvektoren und deren expression in mikrobiellen wirtzellen.
JPS59501572A (ja) * 1982-05-19 1984-09-06 ユニリーバー　ナームローゼ　ベンノートシヤープ クルイベロミセス属酵母およびその製造法
AU577334B2 (en) * 1983-10-11 1988-09-22 International Genetic Engineering, Inc. Synthetic thaumatin genes
ATE253119T1 (de) * 1985-04-15 2003-11-15 Dsm Ip Assets Bv Verwendung des glucoamylasepromotors aus apergillus
CA1310923C (en) * 1985-11-13 1992-12-01 Joachim Ludwig Weickmann Dna encoding [asp --] and [lys -, asp --] thaumatin i
GB2200118A (en) * 1987-01-23 1988-07-27 Allelix Inc Synthetic chymosin-and prochymosin-encoding DNA segments
EP0396612B1 (en) * 1988-01-11 1996-07-24 Xoma Corporation Novel plasmid vector with pectate lyase signal sequence
US5221624A (en) * 1988-11-08 1993-06-22 International Genetic Engineering, Inc. DNA encoding (Lys46, Asp97, Asp113) and (Lys46, Asp.sup.137) thaumatin I polypeptides
JP3986929B2 (ja) * 2002-08-27 2007-10-03 富士通株式会社 電力線搬送通信における漏洩電磁界抑圧送信方法及び漏洩電磁界抑圧送信装置

Also Published As

Publication number Publication date
JPH0847396A (ja) 1996-02-20
DE69517750D1 (de) 2000-08-10
PT684312E (pt) 2000-12-29
FI951868A0 (fi) 1995-04-19
EP0684312B1 (en) 2000-07-05
EP0684312A3 (en) 1996-07-31
CA2147541A1 (en) 1995-10-22
US5932438A (en) 1999-08-03
ATE194386T1 (de) 2000-07-15
ES2080689A1 (es) 1996-02-01
FI951868L (fi) 1995-10-22
DK0684312T3 (da) 2000-11-13
GR3034500T3 (en) 2000-12-29
CA2147541C (en) 2000-10-24
DE69517750T2 (de) 2001-03-01
EP0684312A2 (en) 1995-11-29
FI112794B (fi) 2004-01-15
ES2080689B1 (es) 1996-09-01

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