ES2150411T3 - Vector de integracion de una expresion genica independiente del emplazamiento en las celulas huesped de mamifero. - Google Patents
Vector de integracion de una expresion genica independiente del emplazamiento en las celulas huesped de mamifero.Info
- Publication number
- ES2150411T3 ES2150411T3 ES90903867T ES90903867T ES2150411T3 ES 2150411 T3 ES2150411 T3 ES 2150411T3 ES 90903867 T ES90903867 T ES 90903867T ES 90903867 T ES90903867 T ES 90903867T ES 2150411 T3 ES2150411 T3 ES 2150411T3
- Authority
- ES
- Spain
- Prior art keywords
- gene
- site
- lambda
- downstream
- xho
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K48/00—Medicinal preparations containing genetic material which is inserted into cells of the living body to treat genetic diseases; Gene therapy
Landscapes
- Genetics & Genomics (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Wood Science & Technology (AREA)
- Organic Chemistry (AREA)
- Biomedical Technology (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Zoology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biophysics (AREA)
- Microbiology (AREA)
- Plant Pathology (AREA)
- Molecular Biology (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Physics & Mathematics (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Ultra Sonic Daignosis Equipment (AREA)
- Image Analysis (AREA)
- Devices For Executing Special Programs (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
Abstract
UN VECTOR PARA LA INTEGRACION DE UN GEN EN EL MATERIAL GENETICO DE UNA CELULA HUESPED DE MAMIFERO, TAL QUE EL GEN PUEDE SER EXPRESADO POR LA CELULA HUESPED. EL VECTOR CONSTA DE UN PROMOTOR, EL GEN Y UNA REGION CONTROL INMONOGLOBULINA DOMINANTE DERIVADA DEL LOCUS DEL GEN DE LA INMONOGLOBULINA DEL RATON LAMBDA CAPAZ DE EXTRAER UNA CELULA HUESPED TIPO RESTRICTIVO, UNA POSICION DE INTEGRACION INDEPENDIENTE, UN NUMERO DE COPIAS DEPENDIENTE DE LA EXPRESION DE DICHO GEN. LAS POSICIONES SUPER HIPERSENSIBLES DE DNASAI EJEMPLIFICADAS SON I) CERCA DE 2.35 KB MAS ARRIBA DE LA POSICION CAP DEL GEN LAMBDA, REORDENADO II) CERCA DE 2.5 KB MAS ARRIBA DEL SEGMENTO GENOMICO VLAMBDA SUB 2 O III) CERCA DE 30 KB HACIA ABAJO EL GEN LAMBDA, ADAPTADO. LAS CELULAS HUESPED DE MAMIFEROS TRANSFORMADAS CON EL VECTOR SON REVELADAS COMO SON MAMIFEROS TRANSGENICOS TRANSFORMADOS CON EL VECTOR Y UN METODO DE UNA PRODUCCION DE UN POLIPEPTIDO INCLUYENDO UN CULTIVO DE CELULA TRANSFORMADA DE MAMIFERO. UN METODO DE TERAPIA GENETICA INCLUYE LOS PASOS DE I) ELIMINAR CELULAS TRONCO DEL CUERPO DE UN MAMIFERO, II) OPCIONALMENTE MATAR EL RESTO DE LAS CELULAS TRONCO DEL CUERPO, III) TRANSFORMACION DE LAS CELULAS TRONCO EXTRAIDAS CON EL VECTOR CONTENIENDO UN GEN DEFICIENTE O AUSENTE EN EL CUERPO, Y IV) TAMBIEN SE REVELA LA SUSTITUCION DE LAS CELULAS TRONCO TRANSFORMADAS EN EL CUERPO. TAMBIEN ES REVELADO EL ESTIMULADOR FUNCIONAL DE LA INMUNOGLOBULINA LAMBDA, DEL RATON, CONSISTIENDO EN UNA SECUENCIA DE DNA CONSTANDO DE TODO O UNA PARTE FUNCIONAL DE LA SECUENCIA DE DNA ENTRE LA POSICION ECORI 3.8 KB MAS ABAJO DE LA POSICION XHOI DEL GEN REORDENADO Y LA POSICION SNABI 10 KB MAS ABAJO DE ESTA POSICION XHOI. EL ESTIMULADOR FUNCIONAL DE LA INMUNOGLOBULINA DEL RATON PUEDE CONTENER TODO O UNA PARTE DE I) EL 1.3KB HINDIII PRIMERO HACIA EL FRAGMENTO DE DNA HINDII HACIA ABAJO DE LA POSICION ECORI 3.8 KB HACIA ABAJO DE LA POSICION XHOI EN EL GEN REORDENADO DEL RATON, II) EL 3.3 KB HINDIII HACIA EL FRAGMENTO DE DNA HINDIII HACIA ABAJO DE LA POSICION ECORI 3.8 KB HACIA ABAJO DE LA POSICION XHO I EN EL GEN LAMBDA, REORDENADO DEL RATON Y ABARCANDO LA POSICION SNABI 10 KB HACIA ABAJO DE ESTA POSICION XHOI.
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| GB898904009A GB8904009D0 (en) | 1989-02-22 | 1989-02-22 | Vector |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| ES2150411T3 true ES2150411T3 (es) | 2000-12-01 |
Family
ID=10652102
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| ES90903867T Expired - Lifetime ES2150411T3 (es) | 1989-02-22 | 1990-02-22 | Vector de integracion de una expresion genica independiente del emplazamiento en las celulas huesped de mamifero. |
Country Status (10)
| Country | Link |
|---|---|
| EP (2) | EP0460042B1 (es) |
| JP (1) | JPH04505552A (es) |
| AT (1) | ATE192498T1 (es) |
| AU (2) | AU5161490A (es) |
| CA (2) | CA2297385A1 (es) |
| DE (1) | DE69033531T2 (es) |
| DK (1) | DK0460042T3 (es) |
| ES (1) | ES2150411T3 (es) |
| GB (1) | GB8904009D0 (es) |
| WO (1) | WO1990010077A1 (es) |
Families Citing this family (30)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB9027917D0 (en) * | 1990-12-21 | 1991-02-13 | Ici Plc | Expression systems |
| DE4228162C1 (de) | 1992-08-25 | 1994-01-13 | Rajewsky Klaus Dr | Verfahren zum Ersetzen homologer Genabschnitte aus Säugern in der Keimbahn von nicht-menschlichen Säugern |
| DE19541450C2 (de) | 1995-11-07 | 1997-10-02 | Gsf Forschungszentrum Umwelt | Genkonstrukt und dessen Verwendung |
| US6270747B1 (en) | 1998-08-18 | 2001-08-07 | The University Of California | In vitro and in vivo assay for agents which treat mucus hypersecretion |
| JP2003527833A (ja) | 1999-10-14 | 2003-09-24 | クロンテック・ラボラトリーズ・インコーポレーテッド | 花虫類に由来する発色団／蛍光体、およびそれらの使用法 |
| JP2003000243A (ja) | 2001-06-25 | 2003-01-07 | Takachika Azuma | 変異体の製造法 |
| WO2003054158A2 (en) | 2001-12-19 | 2003-07-03 | The University Of Chicago | Rapidly maturing fluorescent proteins and methods for using the same |
| US7585501B2 (en) | 2002-06-14 | 2009-09-08 | Stowers Institute For Medical Research | Compositions and methods for treating kidney disease |
| CA2525647A1 (en) | 2003-05-16 | 2005-02-24 | Intermune, Inc. | Synthetic chemokine receptor ligands and methods of use thereof |
| WO2005037214A2 (en) | 2003-10-14 | 2005-04-28 | Intermune, Inc. | Macrocyclic carboxylic acids and acylsulfonamides as inhibitors of hcv replication |
| US7407973B2 (en) | 2003-10-24 | 2008-08-05 | Intermune, Inc. | Use of pirfenidone in therapeutic regimens |
| EP1732944B1 (en) | 2004-04-07 | 2012-09-05 | The University of Chicago | Monomeric red fluorescent proteins |
| US7597884B2 (en) | 2004-08-09 | 2009-10-06 | Alios Biopharma, Inc. | Hyperglycosylated polypeptide variants and methods of use |
| US8106013B2 (en) | 2006-05-19 | 2012-01-31 | Georgia Tech Research Corporation | ABC transporter ligand GATX1 |
| EP2049138A4 (en) | 2006-07-14 | 2011-11-09 | Georgia Tech Res Inst | CLC KANALLIGAND |
| US8680235B2 (en) | 2006-09-22 | 2014-03-25 | Stowers Institute For Medical Research | Branchiostoma derived fluorescent proteins |
| WO2009059305A2 (en) | 2007-11-01 | 2009-05-07 | The University Of Chicago | Red fluorescent proteins with enhanced bacterial expression, increased brightness and reduced aggregation |
| EP3025727A1 (en) | 2008-10-02 | 2016-06-01 | The J. David Gladstone Institutes | Methods of treating liver disease |
| JP5827127B2 (ja) | 2008-12-18 | 2015-12-02 | エラスムス・ユニヴァーシティ・メディカル・センター・ロッテルダム | ヒト化抗体を発現する非ヒトトランスジェニック動物及びその使用 |
| EP2482849B1 (en) | 2009-09-30 | 2018-06-06 | Memorial Sloan-Kettering Cancer Center | Combination immunotherapy for the treatment of cancer |
| US10793829B2 (en) | 2010-07-26 | 2020-10-06 | Trianni, Inc. | Transgenic mammals and methods of use thereof |
| CN103025884B (zh) | 2010-07-26 | 2015-11-25 | 特里安尼公司 | 转基因动物和使用方法 |
| US10662256B2 (en) | 2010-07-26 | 2020-05-26 | Trianni, Inc. | Transgenic mammals and methods of use thereof |
| EP3841876A1 (en) | 2013-03-14 | 2021-06-30 | Erasmus University Medical Center Rotterdam | Transgenic mouse for antibody production |
| DK3993623T3 (da) | 2019-07-01 | 2025-05-26 | Zoetis Services Llc | Transgene gnavere og fremgangsmåder til anvendelse deraf |
| CN114502725B (zh) | 2019-07-01 | 2025-09-12 | 特里安尼公司 | 转基因哺乳动物及使用方法 |
| CN111424054B (zh) * | 2020-04-21 | 2021-08-27 | 鲲石生物科技(深圳)有限公司 | 改进的大型载体在人类细胞中传递方法的发现及应用 |
| CN114763558B (zh) | 2021-01-14 | 2024-02-20 | 上海泰槿生物技术有限公司 | 重组基因组、非人哺乳动物细胞及其产生方法和用途 |
| WO2022235759A1 (en) | 2021-05-05 | 2022-11-10 | Trianni, Inc. | Transgenic rodents expressing chimeric equine-rodent antibodies and methods of use thereof |
| KR20240110577A (ko) | 2021-11-10 | 2024-07-15 | 트리아니, 인코포레이티드 | 유전자이식 포유동물 및 이의 사용 방법 |
Family Cites Families (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO1988005083A1 (en) * | 1986-12-24 | 1988-07-14 | Whitehead Institute For Biomedical Research | Method of inducible gene expression |
| DE3724482C2 (de) * | 1987-07-24 | 1995-12-14 | Boehringer Mannheim Gmbh | Verfahren zur Erweiterung der Zellspezifität von eukaryotischen Expressionsvektoren |
-
1989
- 1989-02-22 GB GB898904009A patent/GB8904009D0/en active Pending
-
1990
- 1990-02-22 JP JP2503939A patent/JPH04505552A/ja active Pending
- 1990-02-22 WO PCT/GB1990/000286 patent/WO1990010077A1/en not_active Ceased
- 1990-02-22 AU AU51614/90A patent/AU5161490A/en not_active Abandoned
- 1990-02-22 AT AT90903867T patent/ATE192498T1/de not_active IP Right Cessation
- 1990-02-22 DK DK90903867T patent/DK0460042T3/da active
- 1990-02-22 CA CA002297385A patent/CA2297385A1/en not_active Abandoned
- 1990-02-22 CA CA002050920A patent/CA2050920A1/en not_active Abandoned
- 1990-02-22 EP EP90903867A patent/EP0460042B1/en not_active Expired - Lifetime
- 1990-02-22 DE DE69033531T patent/DE69033531T2/de not_active Expired - Fee Related
- 1990-02-22 EP EP99202794A patent/EP0962534A3/en not_active Withdrawn
- 1990-02-22 ES ES90903867T patent/ES2150411T3/es not_active Expired - Lifetime
-
1994
- 1994-07-26 AU AU68726/94A patent/AU672910B2/en not_active Ceased
Also Published As
| Publication number | Publication date |
|---|---|
| JPH04505552A (ja) | 1992-10-01 |
| GB8904009D0 (en) | 1989-04-05 |
| EP0460042B1 (en) | 2000-05-03 |
| CA2050920A1 (en) | 1990-08-23 |
| EP0460042A1 (en) | 1991-12-11 |
| DE69033531D1 (de) | 2000-06-08 |
| DE69033531T2 (de) | 2001-01-11 |
| CA2297385A1 (en) | 1990-08-23 |
| DK0460042T3 (da) | 2000-07-31 |
| ATE192498T1 (de) | 2000-05-15 |
| EP0962534A2 (en) | 1999-12-08 |
| WO1990010077A1 (en) | 1990-09-07 |
| AU5161490A (en) | 1990-09-26 |
| AU6872694A (en) | 1994-11-17 |
| EP0962534A3 (en) | 1999-12-22 |
| AU672910B2 (en) | 1996-10-17 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| FG2A | Definitive protection |
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