ES2153395T3 - Polimerasa termoestable del acido nucleico. - Google Patents

Polimerasa termoestable del acido nucleico.

Info

Publication number
ES2153395T3
ES2153395T3 ES94106811T ES94106811T ES2153395T3 ES 2153395 T3 ES2153395 T3 ES 2153395T3 ES 94106811 T ES94106811 T ES 94106811T ES 94106811 T ES94106811 T ES 94106811T ES 2153395 T3 ES2153395 T3 ES 2153395T3
Authority
ES
Spain
Prior art keywords
polymerases
polymerase
pyrodictium
activity
whose
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
ES94106811T
Other languages
English (en)
Inventor
David H Gelfand
Alice M Wang
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
F Hoffmann La Roche AG
Original Assignee
F Hoffmann La Roche AG
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by F Hoffmann La Roche AG filed Critical F Hoffmann La Roche AG
Application granted granted Critical
Publication of ES2153395T3 publication Critical patent/ES2153395T3/es
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241—Nucleotidyltransferases (2.7.7)
    • C12N9/1252—DNA-directed DNA polymerase (2.7.7.7), i.e. DNA replicase

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Molecular Biology (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Saccharide Compounds (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

LA INVENCION ES RELATIVA A POLIMERASAS DE DNA TERMOESTABLES PURIFICADAS A PARTIR DE ESPECIES DE PYRODICTIUM, TALES COMO PYRODICTIUM OCCULTUM O PIRODICTIUM ABYSSI, CUYAS POLIMERASAS CATALIZAN LA COMBINACION DE TRIFOSFATOS NUCLEOSIDO PARA FORMAR UNA CADENA DE ACIDO NUCLEICO COMPLEMENTARIA PARA UNA CADENA MODELO DE ACIDO NUCLEICO. LAS POLIMERASAS PREFERIDAS SE CARACTERIZAN POR SU FACULTAD PARA FUNCIONAR EFICIENTEMENTE EN UNA REACCION EN CADENA DE POLIMERASA, EN DONDE DICHA REACCION INCLUYE EXPOSICIONES REPETIDAS A UNA TEMPERATURA DE DESNATURALIZACION DE ALREDEDOR DE 100 ESENTAN ACTIVIDAD EXONUCLEASA 5'' 3'', P.E. SON ENZIMAS DE CORRECCION DE PRUEBAS. LA INVENCION TAMBIEN PROPORCIONA DNAS QUE CODIFICAN LA ACTIVIDAD DE LA POLIMERASA DE DNA DE LAS ESPECIES PYRODICTIUM, CUYOS DNAS PUEDEN SER USADOS PARA CONSTRUIR VECTORES RECOMBINANTES Y CELULAS HUESPED TRANSFORMADAS PARA PRODUCCION DE POLIPEPTIDOS QUE TIENEN DICHA ACTIVIDAD. LA INVENCION TAMBIEN ES RELATIVA A LA PREPARACION DE DICHAS POLIMERASAS DEDNA TERMOESTABLES, AL USO DE DICHAS POLIMERASAS PARA AMPLIFICAR ACIDOS NUCLEICOS ASI COMO A EQUIPOS QUE CONTIENEN UNA POLIMERASA DE LA PRESENTE INVENCION.
ES94106811T 1993-05-14 1994-05-02 Polimerasa termoestable del acido nucleico. Expired - Lifetime ES2153395T3 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US08/062,368 US5491086A (en) 1993-05-14 1993-05-14 Purified thermostable nucleic acid polymerase and DNA coding sequences from pyrodictium species

Publications (1)

Publication Number Publication Date
ES2153395T3 true ES2153395T3 (es) 2001-03-01

Family

ID=22042027

Family Applications (1)

Application Number Title Priority Date Filing Date
ES94106811T Expired - Lifetime ES2153395T3 (es) 1993-05-14 1994-05-02 Polimerasa termoestable del acido nucleico.

Country Status (10)

Country Link
US (1) US5491086A (es)
EP (1) EP0624641B1 (es)
JP (1) JP3626226B2 (es)
AT (1) ATE198083T1 (es)
CA (1) CA2123354C (es)
DE (1) DE69426400T2 (es)
DK (1) DK0624641T3 (es)
ES (1) ES2153395T3 (es)
GR (1) GR3035477T3 (es)
PT (1) PT624641E (es)

Families Citing this family (43)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5756334A (en) * 1990-04-26 1998-05-26 New England Biolabs, Inc. Thermostable DNA polymerase from 9°N-7 and methods for producing the same
US5688668A (en) * 1994-06-15 1997-11-18 Novo Nordisk A/S Pyrodictium xylanase amylase and pullulanase
JP3112148B2 (ja) * 1995-05-31 2000-11-27 東洋紡績株式会社 核酸の増幅方法およびその試薬
US6033859A (en) * 1996-05-24 2000-03-07 Toyo Boseki Kabushiki Kaisha Thermostable DNA polymerase from a hyperthermophilic archaeon strain KOD1
US6001645A (en) * 1995-06-07 1999-12-14 Promega Corporation Thermophilic DNA polymerases from thermotoga neapolitana
US6077664A (en) * 1995-06-07 2000-06-20 Promega Corporation Thermophilic DNA polymerases from Thermotoga neapolitana
CN1552869A (zh) 1995-12-27 2004-12-08 宝酒造株式会社 新型dna聚合酶
US7070962B1 (en) * 1996-02-01 2006-07-04 Aventis Pharmaceuticals Inc. Positive controls in polynucleotide amplification
FR2750999B1 (fr) 1996-07-10 1998-11-20 Appligene Oncor Adn polymerase thermostable d'archaebacteries du genre pyrococcus sp
US6265193B1 (en) * 1997-03-12 2001-07-24 Pe Corporation (Ny) DNA polymerases having improved labeled nucleotide incorporation properties
US7056703B2 (en) 1997-08-06 2006-06-06 Diversa Corporation Polypeptides having polymerase activity and methods of use thereof
US5948666A (en) * 1997-08-06 1999-09-07 Diversa Corporation Isolation and identification of polymerases
US7049101B1 (en) * 1997-08-06 2006-05-23 Diversa Corporation Enzymes having high temperature polymerase activity and methods of use thereof
US5976842A (en) * 1997-10-30 1999-11-02 Clontech Laboratories, Inc. Methods and compositions for use in high fidelity polymerase chain reaction
US6300075B1 (en) * 1999-02-03 2001-10-09 Ortho-Clinical Diagnostics, Inc Enhancement of the specificity of nucleic acid amplification by carrier nucleic acid
ATE278783T1 (de) * 1999-03-06 2004-10-15 Roche Diagnostics Gmbh Dna polymerase aus i(pyrobaculum islandicum)
US6864364B1 (en) * 1999-04-15 2005-03-08 University Of Utah Research Foundation MinK-related genes, formation of potassium channels and association with cardiac arrhythmia
US20040214221A1 (en) * 1999-05-07 2004-10-28 Klaus Muehlegger High density labeling of DNA with modified or "chromophore" carrying nucleotides and DNA polymerases used
US6544750B1 (en) 1999-08-17 2003-04-08 Thromgen, Inc. Peptide analogs as selective inhibitors of thrombin activation of protease activated receptor 1
WO2001061015A2 (en) 2000-02-17 2001-08-23 Qiagen Gmbh Thermostable chimeric nucleic acid polymerases and uses thereof
DE10049211A1 (de) * 2000-10-05 2002-04-18 Qiagen Gmbh Thermostabile Polymerase aus Thermococcus pacificus
CA2426540A1 (en) * 2000-10-20 2002-07-25 Biocardia, Inc. Leukocyte expression profiling
US6509175B2 (en) 2001-04-11 2003-01-21 Incyte Genomics, Inc. cDNA libraries and methods for their production
US6617136B2 (en) 2001-04-24 2003-09-09 3M Innovative Properties Company Biological sample processing methods and compositions that include surfactants
US7026121B1 (en) * 2001-06-08 2006-04-11 Expression Diagnostics, Inc. Methods and compositions for diagnosing and monitoring transplant rejection
US7235358B2 (en) 2001-06-08 2007-06-26 Expression Diagnostics, Inc. Methods and compositions for diagnosing and monitoring transplant rejection
US6905827B2 (en) * 2001-06-08 2005-06-14 Expression Diagnostics, Inc. Methods and compositions for diagnosing or monitoring auto immune and chronic inflammatory diseases
EP1275735A1 (en) * 2001-07-11 2003-01-15 Roche Diagnostics GmbH Composition and method for hot start nucleic acid amplification
US20050042629A1 (en) 2002-09-13 2005-02-24 Applera Corporation Thermus scotoductus nucleic acid polymerases
US7052877B2 (en) 2001-11-30 2006-05-30 Applera Corporation Thermus brockianus nucleic acid polymerases
US7820030B2 (en) * 2003-04-16 2010-10-26 Handylab, Inc. System and method for electrochemical detection of biological compounds
US7892745B2 (en) * 2003-04-24 2011-02-22 Xdx, Inc. Methods and compositions for diagnosing and monitoring transplant rejection
CA2533910A1 (en) * 2003-07-29 2005-02-24 Sigma-Aldrich Co. Methods and compositions for amplification of dna
WO2006029184A2 (en) * 2004-09-08 2006-03-16 Expression Diagnostics, Inc. Genes useful for diagnosing and monitoring inflammation related disorders
EP1885889A4 (en) * 2005-05-11 2010-01-20 Expression Diagnostics Inc METHOD FOR MONITORING THE OPERATING STATUS OF TRANSPLANTS OVER GENLISTS
EP2007909A4 (en) * 2006-04-07 2011-01-26 Xdx Inc EXPRESSION OF NUCLEIC ACID IN RESPONSE TO STEROIDS AND PREDICTION OF DISEASE ACTIVITY
WO2008021431A2 (en) * 2006-08-14 2008-02-21 Xdx, Inc. Methods and compositions for diagnosing and monitoring the status of transplant rejection and immune disorders
US20080124768A1 (en) * 2006-10-31 2008-05-29 Sigma Aldrich Co. Methods and Compositions for Amplification of DNA
US8148067B2 (en) * 2006-11-09 2012-04-03 Xdx, Inc. Methods for diagnosing and monitoring the status of systemic lupus erythematosus
WO2012170907A2 (en) 2011-06-08 2012-12-13 Life Technologies Corporation Polymerization of nucleic acids using proteins having low isoelectric points
FI3461807T3 (fi) 2011-06-08 2023-09-07 Life Technologies Corp Uudenlaisten detergenttien suunnittelu ja kehitys pcr-järjestelmissä käyttöä varten
CN102766611B (zh) * 2012-07-12 2014-02-12 昆明理工大学 硫化叶菌病毒脱氧尿苷焦磷酸酶和编码这种酶的多核苷酸
EP3539944A1 (en) 2013-10-25 2019-09-18 Life Technologies Corporation Novel compounds for use in pcr systems and applications thereof

Family Cites Families (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4683202A (en) * 1985-03-28 1987-07-28 Cetus Corporation Process for amplifying nucleic acid sequences
ES8706823A1 (es) * 1985-03-28 1987-06-16 Cetus Corp Un procedimiento para detectar la presencia o ausencia de al menos una secuencia especifica de acidos nucleicos en una muestra
US4965188A (en) * 1986-08-22 1990-10-23 Cetus Corporation Process for amplifying, detecting, and/or cloning nucleic acid sequences using a thermostable enzyme
CA1338457C (en) * 1986-08-22 1996-07-16 Henry A. Erlich Purified thermostable enzyme
US5407800A (en) * 1986-08-22 1995-04-18 Hoffmann-La Roche Inc. Reverse transcription with Thermus thermophilus polymerase
AU627809B2 (en) * 1988-12-16 1992-09-03 Abbott Laboratories Isolating thermostable enzymes from engineered mesophiles
US5242817A (en) * 1989-09-12 1993-09-07 Johns Hopkins University Proteolytic enzymes from hyperthermophilic bacteria and processes for their production
US5210036A (en) * 1990-04-26 1993-05-11 New England Biolabs, Inc. Purified thermostable DNA polymerase obtainable from thermococcus litoralis
US5352778A (en) * 1990-04-26 1994-10-04 New England Biolabs, Inc. Recombinant thermostable DNA polymerase from archaebacteria
US5322785A (en) * 1990-04-26 1994-06-21 New England Biolabs, Inc. Purified thermostable DNA polymerase obtainable from thermococcus litoralis
US5223414A (en) * 1990-05-07 1993-06-29 Sri International Process for nucleic acid hybridization and amplification
US5210015A (en) * 1990-08-06 1993-05-11 Hoffman-La Roche Inc. Homogeneous assay system using the nuclease activity of a nucleic acid polymerase
WO1992009689A1 (en) * 1990-12-03 1992-06-11 Stratagene PURIFIED THERMOSTABLE $i(PYROCOCCUS FURIOSUS)
US5338671A (en) * 1992-10-07 1994-08-16 Eastman Kodak Company DNA amplification with thermostable DNA polymerase and polymerase inhibiting antibody

Also Published As

Publication number Publication date
EP0624641A2 (en) 1994-11-17
JPH06343466A (ja) 1994-12-20
DE69426400D1 (de) 2001-01-18
DE69426400T2 (de) 2001-06-21
EP0624641A3 (en) 1995-01-11
PT624641E (pt) 2001-04-30
CA2123354A1 (en) 1994-11-15
CA2123354C (en) 2006-11-28
DK0624641T3 (da) 2001-04-17
JP3626226B2 (ja) 2005-03-02
US5491086A (en) 1996-02-13
GR3035477T3 (en) 2001-05-31
EP0624641B1 (en) 2000-12-13
ATE198083T1 (de) 2000-12-15

Similar Documents

Publication Publication Date Title
ES2153395T3 (es) Polimerasa termoestable del acido nucleico.
Maki et al. MutT protein specifically hydrolyses a potent mutagenic substrate for DNA synthesis
Alderton et al. Heat adaptation and ion exchange in Bacillus megaterium spores
ATE172493T1 (de) Rekombinante dns-mittel und verfahren
NO173741C (no) DNA-sekvens for modifisert protein C, vektor inneholdende denne, patte-dyrceller transfektert med vektorer, og fremgangsmaate for fremstilling av nevnte protein
ATE526408T1 (de) Modulatoren von dem körpergewicht, entsprechende nukleinsaüre und proteine, und diagnostische und therapeutische anwendungen davon
DE69331550D1 (de) Klonierung und expression von biologisch aktiver alpha-galaktosidase a
CA2079454A1 (en) Steroid 5.alpha.-reductases
DK0559884T3 (da) Rekombinante virusvektorer til ekspression i muskelceller
ATE181106T1 (de) Mutationen in der 5'-3'-exonukleaseaktivität thermostabiler dna-polymerasen
BR0010114A (pt) Vetor biologicamente funcional, molécula de dna, molécula de dna codificada para ribozima, método de clonagem de uma transferase g1cnac-a-1.3-fucosil, método de preparação de células hospedeiras recombinantes, células de plantas recombinantes, insetos ou células de insetos recombinantes, molécula de ácido nucléico de peptìdeo (pna), método de produção de plantas ou insetos ou células, método de produção de glicoproteìnas recombinantes, método de produção de glicoproteìnas humanas, recombinantes, método de produção de gligoproteìnas humanas, recombinantes para uso médico, método de seleção de moléculas de dna codificada para uma transferase g1cnac-a-1,-3-fucosil em uma amostra
BR9711556A (pt) Planta geneticamente engenhada, processo de produção de uma planta, proteìna hemoglobina de vitreoscilla mutante, polinucleotìdeo, célula hospedeira, e, processos para gerar um polinucleotìdeo codificador de proteìna hemoglobina de vitreoscilla mutante e de alteraçào da distribuição de metabólitos de secundários em uma planta.
AR016059A1 (es) Proteina sustancialmente purificada que presenta propiedades insecticidas, secuencias de adn, vector, molecula aislada de nucleotidos, organismo, metodopara controlar el gusano de la raiz del maiz, celula vegetal transformada.
HUP9800329A2 (hu) Nukleinsavkonstrukciók strukturális gének sejtciklus-függő módon szabályozott expresszálására
DE3751632D1 (de) Expression von biologisch-aktiven PDGF-Analogen in eukaryotischen Zellen
ATE113982T1 (de) Verzweigungsenzym und dessen verwendung.
DK87190A (da) Fremgangsmaade til at regulere plantevaekst under anvendelse af svovlholdige organiske syrer
KR960017845A (ko) 부위-지정 변이유발방법
ATE80912T1 (de) Menschliche proteine des chinolinesterase-typs und ihre herstellung.
DK513689D0 (da) Gen for bacillus thurigiensis p-2 toksin, fremgangsmaade til fremstilling af toksinet, rekombinant vektor indeholdende genet og plante, der er transformeret med genets dna-sekvens eller en del eller et derivat deraf
BR9914130A (pt) Promotores de plantas e terminadores de plantas
EP1728859A4 (en) SEQUENCE CAPABLE OF ACCELERATING GENE EXPRESSION AT A MODERATELY LOW TEMPERATURE
AR038137A1 (es) Composicion que comprende un polipeptido de hidroxilasa de acido graso lesquerella modificado, molecula de adn recombinante, vector de expresion y organismo transformado con la molecula de adn recombinante; planta, celula de planta y semilla de planta transformada; aceite obtenido de dicha semilla;
WO1997045547A3 (de) Lokalisierter zelltod in pflanzen
BR9916831A (pt) Genes do ciclo celular e seu uso

Legal Events

Date Code Title Description
FG2A Definitive protection

Ref document number: 624641

Country of ref document: ES