ES2155379A1 - Procedimiento para producir plaquetas humanas a partir de celulas en cultivo. - Google Patents

Procedimiento para producir plaquetas humanas a partir de celulas en cultivo.

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Publication number
ES2155379A1
ES2155379A1 ES9900666A ES9900666A ES2155379A1 ES 2155379 A1 ES2155379 A1 ES 2155379A1 ES 9900666 A ES9900666 A ES 9900666A ES 9900666 A ES9900666 A ES 9900666A ES 2155379 A1 ES2155379 A1 ES 2155379A1
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Spain
Prior art keywords
plaquettes
human
platelets
blood
cell lines
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
ES9900666A
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English (en)
Other versions
ES2155379B1 (es
Inventor
Serrano Javier Leon
Flamarique Ana Lerga
Espiga Carlos Richard
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Universidad de Cantabria
Original Assignee
Universidad de Cantabria
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Filing date
Publication date
Application filed by Universidad de Cantabria filed Critical Universidad de Cantabria
Priority to ES9900666A priority Critical patent/ES2155379B1/es
Publication of ES2155379A1 publication Critical patent/ES2155379A1/es
Application granted granted Critical
Publication of ES2155379B1 publication Critical patent/ES2155379B1/es
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

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  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

Procedimiento para producir plaquetas humanas a partir de células en cultivo. Se describe un procedimiento para obtener cantidades ilimitadas de plaquetas humanas a partir de las líneas celulares K562 y KU812, derivadas de leucemias mieloides. El procedimiento se basa en el tratamiento con estaurosporina a concentraciones 50 a 100 nM durante 3 a 6 días. Las células pueden crecerse en suspensión y las plaquetas pueden ser separadas de las células por métodos estándar. Las plaquetas son reconocibles morfológicamente y comparten características bioquímicas y ultraestructurales con las plaquetas aisladas de sangre. Se puede obtener así una preparación de plaquetas humanas a partir de precursores celulares genéticamente idénticos y libre de otros productos biológicos de origen humano. Pueden ser usadas para fines analíticos como servir de estándar en ensayos plaquetarios biológicos o bioquímicos.
ES9900666A 1999-04-05 1999-04-05 Procedimiento para producir plaquetas humanas a partir de celulas en cultivo. Expired - Fee Related ES2155379B1 (es)

Priority Applications (1)

Application Number Priority Date Filing Date Title
ES9900666A ES2155379B1 (es) 1999-04-05 1999-04-05 Procedimiento para producir plaquetas humanas a partir de celulas en cultivo.

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
ES9900666A ES2155379B1 (es) 1999-04-05 1999-04-05 Procedimiento para producir plaquetas humanas a partir de celulas en cultivo.

Publications (2)

Publication Number Publication Date
ES2155379A1 true ES2155379A1 (es) 2001-05-01
ES2155379B1 ES2155379B1 (es) 2001-12-01

Family

ID=8307875

Family Applications (1)

Application Number Title Priority Date Filing Date
ES9900666A Expired - Fee Related ES2155379B1 (es) 1999-04-05 1999-04-05 Procedimiento para producir plaquetas humanas a partir de celulas en cultivo.

Country Status (1)

Country Link
ES (1) ES2155379B1 (es)

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ES2136103T3 (es) * 1992-06-22 1999-11-16 Kyowa Hakko Kogyo Kk Procedimiento para la preparacion de derivados de estaurosporina.

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ES2136103T3 (es) * 1992-06-22 1999-11-16 Kyowa Hakko Kogyo Kk Procedimiento para la preparacion de derivados de estaurosporina.

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
ADACHI M. et al. "Induction of smg p21/rap1A p21/krev-1 p21 gen expression during phorbol ester-induced differentiation of a human megakaryocytic leukemia cell line". 1992. Oncogene. Vol. 7 (2), paginas 323-9. *
MURGO A. et al. "K562 leukemia cells express P2T (adenosine diphosphate) purinergic receptors". 1992. J. Pharmacol. Exp. Ther., Vol. 261 (2), paginas 580-5. *
YEN A. et al. "12-O-Tetradecanoylphorbol-13-acetate and staurosporine induce increased retinoblastoma tumor suppressor gene expression with megakaryocytic differentiation of leukemia cells". 1993. Cancer Res., Vol. 53 (13), paginas 3085-91. *

Also Published As

Publication number Publication date
ES2155379B1 (es) 2001-12-01

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