ES2167620T3 - Procedimiento para la determinacion de la expresion del ligando cd95 en celulas. - Google Patents

Procedimiento para la determinacion de la expresion del ligando cd95 en celulas.

Info

Publication number
ES2167620T3
ES2167620T3 ES96945752T ES96945752T ES2167620T3 ES 2167620 T3 ES2167620 T3 ES 2167620T3 ES 96945752 T ES96945752 T ES 96945752T ES 96945752 T ES96945752 T ES 96945752T ES 2167620 T3 ES2167620 T3 ES 2167620T3
Authority
ES
Spain
Prior art keywords
binding
procedure
determination
expression
cells
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
ES96945752T
Other languages
English (en)
Inventor
Klaus-Michael Debatin
Ingrid Herr
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Deutsches Krebsforschungszentrum DKFZ
Original Assignee
Deutsches Krebsforschungszentrum DKFZ
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Deutsches Krebsforschungszentrum DKFZ filed Critical Deutsches Krebsforschungszentrum DKFZ
Application granted granted Critical
Publication of ES2167620T3 publication Critical patent/ES2167620T3/es
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6883—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6851—Quantitative amplification
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/158—Expression markers

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Analytical Chemistry (AREA)
  • Zoology (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Pathology (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)

Abstract

LA PRESENTE INVENCION TRATA DE UN PROCEDIMIENTO DESTINADO A LA DETERMINACION CUANTITATIVA DEL LIGANDO CD95 Y COMPRENDE LOS SIGUIENTES PASOS: (A) AISLAMIENTO DEL ARN TOTAL DE LAS CELULAS; (B) TRANSCRIPCION DEL ARN TOTAL DE (A) EN CADN MEDIANTE TRANSCRIPCION INVERSA; Y (C) AMPLIFICACION DEL CADN DE (B) Y DE UN FRAGMENTO COMPETIDOR DE LIGANDO CD95 MEDIANTE UN PRIMER ESPECIFICO DEL LIGANDO CD95 EN UNA REACCION DE PCR. EL PROCEDIMIENTO ES APTO PARA DETERMINAR LA MAGNITUD Y/O EL CURSO DE LA APOPTOSIS.
ES96945752T 1995-11-28 1996-11-27 Procedimiento para la determinacion de la expresion del ligando cd95 en celulas. Expired - Lifetime ES2167620T3 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
DE19544332A DE19544332C2 (de) 1995-11-28 1995-11-28 Verfahren zum Nachweis der Expression von CD95 Ligand in Zellen

Publications (1)

Publication Number Publication Date
ES2167620T3 true ES2167620T3 (es) 2002-05-16

Family

ID=7778609

Family Applications (1)

Application Number Title Priority Date Filing Date
ES96945752T Expired - Lifetime ES2167620T3 (es) 1995-11-28 1996-11-27 Procedimiento para la determinacion de la expresion del ligando cd95 en celulas.

Country Status (8)

Country Link
US (1) US6153385A (es)
EP (1) EP0870061B1 (es)
JP (1) JP2000500657A (es)
AT (1) ATE207129T1 (es)
DE (2) DE19544332C2 (es)
DK (1) DK0870061T3 (es)
ES (1) ES2167620T3 (es)
WO (1) WO1997020067A2 (es)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1087993B1 (en) 1998-06-18 2008-04-02 Imed Ab Fas peptides and antibodies for modulating apoptosis

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5219727A (en) * 1989-08-21 1993-06-15 Hoffmann-Laroche Inc. Quantitation of nucleic acids using the polymerase chain reaction
JPH08127594A (ja) * 1993-11-10 1996-05-21 Mochida Pharmaceut Co Ltd Ｆａｓ抗原に結合する新規蛋白質およびそれをコードするＤＮＡ

Also Published As

Publication number Publication date
EP0870061A2 (de) 1998-10-14
EP0870061B1 (de) 2001-10-17
WO1997020067A3 (de) 1997-10-02
US6153385A (en) 2000-11-28
DE19544332A1 (de) 1997-06-05
DE59607965D1 (de) 2001-11-22
WO1997020067A2 (de) 1997-06-05
ATE207129T1 (de) 2001-11-15
DE19544332C2 (de) 1999-07-08
DK0870061T3 (da) 2002-02-11
JP2000500657A (ja) 2000-01-25

Similar Documents

Publication Publication Date Title
BR9801878A (pt) Iniciadores de amplificação de ácido nucleìco modificado
Duarte et al. Repair and mutagenic potential of oxaluric acid, a major product of singlet oxygen-mediated oxidation of 8-oxo-7, 8-dihydroguanine
Palom et al. Relative toxicities of DNA cross-links and monoadducts: new insights from studies of decarbamoyl mitomycin C and mitomycin C
Samiec et al. Expression of pluripotency‐related genes is highly dependent on trichostatin A‐assisted epigenomic modulation of porcine mesenchymal stem cells analysed for apoptosis and subsequently used for generating cloned embryos
Oh et al. Anti‐senescence effects of DNA methyltransferase inhibitor RG108 in human bone marrow mesenchymal stromal cells
EP1185546A4 (en) HIGHLY SPECIFIC PRIMERS, EXPRESSION PROCEDURES AND "KIT"
Miller et al. Stereoselective excision of thymine glycol from oxidatively damaged DNA
ES2721631T3 (es) Método para aislar pequeñas moléculas de ARN
BR0205268A (pt) Processos e composições para a mplificação de sequências de rna
NO20076430L (no) 4c
NO20022359L (no) Inhibering av genekspresjon
MX2021003625A (es) Complejos de transposoma ligados a superficie de complejo.
BRPI0510811A (pt) nano-pcr: métodos e dispositivos para amplificação e detecção de ácido nucléico
AR025754A1 (es) Secretasa en la enfermedad de alzheimer, sustratos de la app y sus usos
AU8023898A (en) Combined product associating a nucleic acid with a substance breaking up the extracellular matrix for gene therapy
WO2008011157A3 (en) Methods, compositions, and kits for the selective activation of protoxins through combinatorial targeting
DE60122138D1 (de) Isolierung von nukleinsäuren
Kolb et al. Hexitol nucleic acid-containing aptamers are efficient ligands of HIV-1 TAR RNA
Yamamoto et al. Characterization of rapid and high‐affinity uptake of L‐serine in neurons and astrocytes in primary culture
AR020110A1 (es) Construccion; plastido de celula de planta; planta, semilla de planta, celula de planta o progenie de la misma; metodo para producir tolerancia a unherbicida en una celula de planta
PT942999E (pt) Construcao armadilha de genes para a identificacao e isolamento de genes
Bhattacharyya et al. DnaB helicase affects the initiation specifity of Escherichia coli primase on single-stranded DNA templates
ES473276A1 (es) Procedimiento para la fermentacion continua de microorganis-mos.
ES2167620T3 (es) Procedimiento para la determinacion de la expresion del ligando cd95 en celulas.
DK1003525T3 (da) Præparat og fremgangsmåde til regulering af celleproliferation og celledød