ES2168509T3 - Procedimientos de determinacion in vitro de la bioactividad de la eritropoyetina. - Google Patents

Procedimientos de determinacion in vitro de la bioactividad de la eritropoyetina.

Info

Publication number
ES2168509T3
ES2168509T3 ES96933065T ES96933065T ES2168509T3 ES 2168509 T3 ES2168509 T3 ES 2168509T3 ES 96933065 T ES96933065 T ES 96933065T ES 96933065 T ES96933065 T ES 96933065T ES 2168509 T3 ES2168509 T3 ES 2168509T3
Authority
ES
Spain
Prior art keywords
epo
sample
vitro
cells
activity
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
ES96933065T
Other languages
English (en)
Inventor
Peter J Lisi
Jeffrey K Glenn
Chi-Kwong So
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Janssen Pharmaceuticals Inc
Original Assignee
Ortho McNeil Pharmaceutical Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Ortho McNeil Pharmaceutical Inc filed Critical Ortho McNeil Pharmaceutical Inc
Application granted granted Critical
Publication of ES2168509T3 publication Critical patent/ES2168509T3/es
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • G01N33/5008—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/74—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving hormones or other non-cytokine intercellular protein regulatory factors such as growth factors, including receptors to hormones and growth factors
    • G01N33/746—Erythropoetin

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Immunology (AREA)
  • Chemical & Material Sciences (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Food Science & Technology (AREA)
  • General Physics & Mathematics (AREA)
  • Cell Biology (AREA)
  • Biotechnology (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Pathology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Toxicology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Endocrinology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Investigating Or Analysing Biological Materials (AREA)

Abstract

LA PRESENTE INVENCION SE REFIERE A UN PROCEDIMIENTO IN VITRO DE DETERMINACION DE LA ACTIVIDAD IN VIVO DE LA ERITROPOYETINA (EPO) PRESENTE EN UNA MUESTRA. MAS ESPECIALMENTE, ESTE PROCEDIMIENTO CONSISTE EN TRATAR UNA MUESTRA QUE CONTIENE EPO EN UNAS CONDICIONES QUE SUPRIMEN EL EPO DESIALILADO Y EN MEDIR LA ACTIVIDAD IN VITRO DE LA EPO DE LA MUESTRA TRATADA RESULTANTE. EN UNA REALIZACION PREFERIDA, LA EPO DESIALILADA SE SUPRIME DE LA MUESTRA POR INCUBACION DE DICHA MUESTRA CON CELULAS DE LA LINEA CELULAR DEL HEPATOCARCINOMA HUMANO HEPG2, Y SE DETERMINA LA ACTIVIDAD IN VITRO DE LA EPO POR INCUBACION DE LA MUESTRA TRATADA CON CELULAS DE UNA LINEA CELULAR QUE REACCIONA A LA EPO, Y POR MEDIDA DE LA PROLIFERACION O DE LA VIABILIDAD DE LAS CELULAS QUE REACCIONAN A LA EPO. LA PRESENTE INVENCION ES UTIL, POR EJEMPLO, PARA LA CUANTIFICACION DE LA EPO BIOLOGICAMENTE ACTIVA EN TIPOS DE MUESTRAS DIFERENTES.
ES96933065T 1996-09-20 1996-09-20 Procedimientos de determinacion in vitro de la bioactividad de la eritropoyetina. Expired - Lifetime ES2168509T3 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
PCT/US1996/015049 WO1998012558A1 (en) 1996-09-20 1996-09-20 Methods for in vitro determination of erythropoietin bioactivity

Publications (1)

Publication Number Publication Date
ES2168509T3 true ES2168509T3 (es) 2002-06-16

Family

ID=22255824

Family Applications (1)

Application Number Title Priority Date Filing Date
ES96933065T Expired - Lifetime ES2168509T3 (es) 1996-09-20 1996-09-20 Procedimientos de determinacion in vitro de la bioactividad de la eritropoyetina.

Country Status (13)

Country Link
EP (1) EP0935752B1 (es)
JP (1) JP3764487B2 (es)
KR (1) KR100295506B1 (es)
AT (1) ATE209354T1 (es)
AU (1) AU715237B2 (es)
CA (1) CA2265989C (es)
DE (1) DE69618630T2 (es)
DK (1) DK0935752T3 (es)
ES (1) ES2168509T3 (es)
IL (1) IL129028A0 (es)
NO (1) NO321047B1 (es)
PT (1) PT935752E (es)
WO (1) WO1998012558A1 (es)

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
IL73544A (en) * 1984-11-19 1988-11-15 Yissum Res Dev Co In vitro bioassay of erythropoietin in the absence of serum involving dna synthesis by erythroid precursors
AU4104889A (en) * 1988-09-07 1990-03-15 Bioclones (Pty) Ltd Purification of erythropoietin
JP2750600B2 (ja) * 1989-02-17 1998-05-13 雪印乳業株式会社 エリスロポエチンの生物活性測定方法
JPH05502463A (ja) * 1990-09-28 1993-04-28 オーソ・フアーマシユーチカル・コーポレーシヨン ハイブリツド成長因子

Also Published As

Publication number Publication date
DE69618630T2 (de) 2002-05-08
NO991338L (no) 1999-03-19
NO321047B1 (no) 2006-03-06
JP3764487B2 (ja) 2006-04-05
DK0935752T3 (da) 2002-05-21
EP0935752B1 (en) 2001-11-21
NO991338D0 (no) 1999-03-19
IL129028A0 (en) 2000-02-17
KR100295506B1 (ko) 2001-08-07
WO1998012558A1 (en) 1998-03-26
CA2265989C (en) 2010-11-02
AU7162696A (en) 1998-04-14
CA2265989A1 (en) 1998-03-26
EP0935752A1 (en) 1999-08-18
AU715237B2 (en) 2000-01-20
ATE209354T1 (de) 2001-12-15
JP2001500736A (ja) 2001-01-23
PT935752E (pt) 2002-03-28
DE69618630D1 (de) 2002-02-21
KR20000048492A (ko) 2000-07-25

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