ES2170797T3 - Procedimiento de amplificacion enzimatica (in vitro) de un fragmento de adn con la ayuda de iniciadores en escalera. - Google Patents

Procedimiento de amplificacion enzimatica (in vitro) de un fragmento de adn con la ayuda de iniciadores en escalera.

Info

Publication number
ES2170797T3
ES2170797T3 ES95920951T ES95920951T ES2170797T3 ES 2170797 T3 ES2170797 T3 ES 2170797T3 ES 95920951 T ES95920951 T ES 95920951T ES 95920951 T ES95920951 T ES 95920951T ES 2170797 T3 ES2170797 T3 ES 2170797T3
Authority
ES
Spain
Prior art keywords
staircase
dna fragment
enzymatic amplification
vitro
amplification procedure
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
ES95920951T
Other languages
English (en)
Inventor
Ronald Colimon
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Argene SA
Original Assignee
Argene SA
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Argene SA filed Critical Argene SA
Application granted granted Critical
Publication of ES2170797T3 publication Critical patent/ES2170797T3/es
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/686—Polymerase chain reaction [PCR]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6858—Allele-specific amplification
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/70—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving virus or bacteriophage
    • C12Q1/701—Specific hybridization probes
    • C12Q1/702—Specific hybridization probes for retroviruses
    • C12Q1/703—Viruses associated with AIDS

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Virology (AREA)
  • AIDS & HIV (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

LA PRESENTE INVENCION SE REFIERE A UN PROCEDIMIENTO DE AMPLIFICACION ENZIMATICA IN VITRO DE UN FRAGMENTO DE ADN EN EL QUE SE UTILIZAN DOS CEBOS RESPECTIVAMENTE CORRIENTE ARRIBA Y CORRIENTE ABAJO DE LOS QUE UNO AL MENOS ES UN CEBO LLAMADO EN "ESCALERA" QUE CONSISTE EN UNA MEZCLA DE OLIGONUCLEOTIDOS, ESTANDO LOS OLIGONUCLEOTIDOS QUE CONSTITUYEN LA MEZCLA EN CANTIDADES EQUIMOLARES, DE TAMAÑOS DIFERENTES, DE SECUENCIAS COMPLEMENTARIAS A LA MISMA REGION CORRIENTE ARRIBA O CORRIENTE ABAJO DE DICHO FRAGMENTO A AMPLIFICAR Y TENIENDO CADA OLIGONUCLEOTIDO, A PARTIR DE SU EXTREMO 5'', EN EL SENTIDO 5'' HACIA 3'', LA MISMA SECUENCIA QUE LOS OLIGONUCLEOTIDOS MAS PEQUEÑOS DE LA MEZCLA. LA PRESENTE INVENCION SE REFIERE A UN PROCEDIMIENTO DE DIAGNOSTICO Y A UN KIT DE DIAGNOSTICO, PARTICULARMENTE DE ENFERMEDADES VIRALES.
ES95920951T 1994-05-18 1995-05-18 Procedimiento de amplificacion enzimatica (in vitro) de un fragmento de adn con la ayuda de iniciadores en escalera. Expired - Lifetime ES2170797T3 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
FR9406055A FR2720077B1 (fr) 1994-05-18 1994-05-18 Procédé d'amplification enzymatique in vitro d'un fragment d'ADN à l'aide d'amorces en escalier.

Publications (1)

Publication Number Publication Date
ES2170797T3 true ES2170797T3 (es) 2002-08-16

Family

ID=9463303

Family Applications (1)

Application Number Title Priority Date Filing Date
ES95920951T Expired - Lifetime ES2170797T3 (es) 1994-05-18 1995-05-18 Procedimiento de amplificacion enzimatica (in vitro) de un fragmento de adn con la ayuda de iniciadores en escalera.

Country Status (5)

Country Link
EP (1) EP0720660B1 (es)
DE (1) DE69525435T2 (es)
ES (1) ES2170797T3 (es)
FR (1) FR2720077B1 (es)
WO (1) WO1995031568A2 (es)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2012131616A1 (en) * 2011-03-30 2012-10-04 Chromous Biotech Pvt. Ltd. Primer set, kit and methods thereof
US10100349B2 (en) * 2013-09-30 2018-10-16 President And Fellows Of Harvard College Methods of determining polymorphisms

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2013317A1 (en) * 1989-04-17 1990-10-17 Fred T. Oakes Diagnostic kit, primer composition and their use for replication or detection of nucleic acids
US5196305A (en) * 1989-09-12 1993-03-23 Eastman Kodak Company Diagnostic and amplification methods using primers having thymine at 3' end to overcome primer-target mismatch at the 3' end
IE66125B1 (en) * 1991-01-31 1995-12-13 Zeneca Ltd Detection method for variant nucleotides
EP0592546B1 (en) * 1991-06-14 2003-05-28 Virogenetics Corporation Immunodeficiency virus recombinant poxvirus vaccine

Also Published As

Publication number Publication date
EP0720660B1 (fr) 2002-02-13
DE69525435T2 (de) 2002-10-31
FR2720077B1 (fr) 1996-08-09
FR2720077A1 (fr) 1995-11-24
WO1995031568A3 (fr) 1995-12-14
DE69525435D1 (de) 2002-03-21
EP0720660A1 (fr) 1996-07-10
WO1995031568A2 (fr) 1995-11-23

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