ES2172380B1 - Un procedimiento para producir alfa-galactosidasa recombinante de thermus sp. t2 en celulas hospedantes. - Google Patents
Un procedimiento para producir alfa-galactosidasa recombinante de thermus sp. t2 en celulas hospedantes.Info
- Publication number
- ES2172380B1 ES2172380B1 ES200000515A ES200000515A ES2172380B1 ES 2172380 B1 ES2172380 B1 ES 2172380B1 ES 200000515 A ES200000515 A ES 200000515A ES 200000515 A ES200000515 A ES 200000515A ES 2172380 B1 ES2172380 B1 ES 2172380B1
- Authority
- ES
- Spain
- Prior art keywords
- alpha
- thermus
- cells
- galactosidase
- hospeding
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
- 238000000034 method Methods 0.000 title abstract 4
- 241000589497 Thermus sp. Species 0.000 title abstract 3
- 108010030291 alpha-Galactosidase Proteins 0.000 abstract 4
- 102000005840 alpha-Galactosidase Human genes 0.000 abstract 3
- 239000002773 nucleotide Substances 0.000 abstract 3
- 125000003729 nucleotide group Chemical group 0.000 abstract 3
- 241000588724 Escherichia coli Species 0.000 abstract 2
- 102000007056 Recombinant Fusion Proteins Human genes 0.000 abstract 1
- 108010008281 Recombinant Fusion Proteins Proteins 0.000 abstract 1
- 125000003275 alpha amino acid group Chemical group 0.000 abstract 1
- 238000010367 cloning Methods 0.000 abstract 1
- 230000000694 effects Effects 0.000 abstract 1
- 239000013604 expression vector Substances 0.000 abstract 1
- 239000000284 extract Substances 0.000 abstract 1
- 235000013305 food Nutrition 0.000 abstract 1
- 230000002068 genetic effect Effects 0.000 abstract 1
- 230000007062 hydrolysis Effects 0.000 abstract 1
- 238000006460 hydrolysis reaction Methods 0.000 abstract 1
- 238000012986 modification Methods 0.000 abstract 1
- 230000004048 modification Effects 0.000 abstract 1
- 108090000623 proteins and genes Proteins 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
- C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
- C12N9/2465—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1) acting on alpha-galactose-glycoside bonds, e.g. alpha-galactosidase (3.2.1.22)
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Molecular Biology (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Biomedical Technology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Medicinal Chemistry (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Enzymes And Modification Thereof (AREA)
Abstract
Un procedimiento para producir alfa-galactosidasa recombinante de Thermus sp. T2 en células hospedantes. Se describe una secuencia de nucleótidos que corresponde a un gen estructural de Thermus sp. T2 ATCC 27737 que codifica para una alfa-galactosidasa (EC 3.2.1.22) termoestable. Se indica el modo de obtener una construcción genética para la expresión de dicha secuencia de nucleótidos en células de Escherichia coli mediante su clonación en un vector de expresión, con las modificaciones correspondientes en las secuencias de nucleótidos y de aminoácidos a las que la manipulación aboca. Se describe un método para obtener extractos enriquecidos con actividad alfa- galactosidasa termoestable producida en E. coli. La proteína recombinante descrita, debido a su característica de termoestabilidad, que la hace compatible con diversos procedimientos tecnológicos, tiene aplicación principalmente en el sector agroalimentario-sanitario, para la hidrólisis de alfa- galactósidos de mala digestibilidad presentes en diversos alimentos de gran consumo.
Priority Applications (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| ES200000515A ES2172380B1 (es) | 2000-03-03 | 2000-03-03 | Un procedimiento para producir alfa-galactosidasa recombinante de thermus sp. t2 en celulas hospedantes. |
| PCT/ES2001/000078 WO2001064914A1 (es) | 2000-03-03 | 2001-03-02 | Un procedimiento para producir alfa-galactosidasa recombinante dethermus sp. t2 en celulas hospedantes |
| AU37444/01A AU3744401A (en) | 2000-03-03 | 2001-03-02 | Method for producing a recombinant alpha-galactosidase of thermus sp. t2 in hostcells |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| ES200000515A ES2172380B1 (es) | 2000-03-03 | 2000-03-03 | Un procedimiento para producir alfa-galactosidasa recombinante de thermus sp. t2 en celulas hospedantes. |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| ES2172380A1 ES2172380A1 (es) | 2002-09-16 |
| ES2172380B1 true ES2172380B1 (es) | 2003-12-01 |
Family
ID=8492553
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| ES200000515A Expired - Fee Related ES2172380B1 (es) | 2000-03-03 | 2000-03-03 | Un procedimiento para producir alfa-galactosidasa recombinante de thermus sp. t2 en celulas hospedantes. |
Country Status (3)
| Country | Link |
|---|---|
| AU (1) | AU3744401A (es) |
| ES (1) | ES2172380B1 (es) |
| WO (1) | WO2001064914A1 (es) |
Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| ES2351296B8 (es) | 2009-04-08 | 2012-07-03 | Universidade Da Coruña | Cepas de s. cerevisiae capaces de crecer en medios con melibiosa, estaquiosa y rafinosa. |
| CN108359655B (zh) * | 2018-02-08 | 2020-04-24 | 刘丹妮 | 一种热稳定性高的脂肪酶突变体TDL-mut及其编码基因 |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| ES2137115B1 (es) * | 1997-08-07 | 2000-08-16 | Consejo Superior Investigacion | Un procedimiento para producir beta-galactosidasa recombinante de thermus sp. (cepa t2) en celulas hospedantes, y purificarla en un solo paso cromatografico. |
-
2000
- 2000-03-03 ES ES200000515A patent/ES2172380B1/es not_active Expired - Fee Related
-
2001
- 2001-03-02 AU AU37444/01A patent/AU3744401A/en not_active Abandoned
- 2001-03-02 WO PCT/ES2001/000078 patent/WO2001064914A1/es not_active Ceased
Also Published As
| Publication number | Publication date |
|---|---|
| WO2001064914A1 (es) | 2001-09-07 |
| AU3744401A (en) | 2001-09-12 |
| ES2172380A1 (es) | 2002-09-16 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| EC2A | Search report published |
Date of ref document: 20020916 Kind code of ref document: A1 Effective date: 20020916 |
|
| FD1A | Patent lapsed |
Effective date: 20100528 |