ES2173670T3 - Procedimiento de identificacion de acidos nucleicos mediante espectrometria de masas laser de desorcion/ionizacion asistidas por matriz. - Google Patents

Procedimiento de identificacion de acidos nucleicos mediante espectrometria de masas laser de desorcion/ionizacion asistidas por matriz.

Info

Publication number
ES2173670T3
ES2173670T3 ES98966608T ES98966608T ES2173670T3 ES 2173670 T3 ES2173670 T3 ES 2173670T3 ES 98966608 T ES98966608 T ES 98966608T ES 98966608 T ES98966608 T ES 98966608T ES 2173670 T3 ES2173670 T3 ES 2173670T3
Authority
ES
Spain
Prior art keywords
probes
matrix
nucleic acid
desortion
nucleic acids
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
ES98966608T
Other languages
English (en)
Inventor
Ivo Glynne Gut
Kurt Berlin
Hans Lehrach
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Max Planck Gesellschaft zur Foerderung der Wissenschaften eV
Original Assignee
Max Planck Gesellschaft zur Foerderung der Wissenschaften eV
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Max Planck Gesellschaft zur Foerderung der Wissenschaften eV filed Critical Max Planck Gesellschaft zur Foerderung der Wissenschaften eV
Application granted granted Critical
Publication of ES2173670T3 publication Critical patent/ES2173670T3/es
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
    • C12Q1/6872—Methods for sequencing involving mass spectrometry
    • H—ELECTRICITY
    • H01—ELECTRIC ELEMENTS
    • H01J—ELECTRIC DISCHARGE TUBES OR DISCHARGE LAMPS
    • H01J49/00—Particle spectrometers or separator tubes
    • H01J49/02—Details
    • H01J49/04—Arrangements for introducing or extracting samples to be analysed, e.g. vacuum locks; Arrangements for external adjustment of electron- or ion-optical components
    • H01J49/0409—Sample holders or containers
    • H01J49/0418—Sample holders or containers for laser desorption, e.g. matrix-assisted laser desorption/ionisation [MALDI] plates or surface enhanced laser desorption/ionisation [SELDI] plates

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Spectroscopy & Molecular Physics (AREA)
  • Immunology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Other Investigation Or Analysis Of Materials By Electrical Means (AREA)
  • Investigating Or Analysing Biological Materials (AREA)

Abstract

Procedimiento para identificar una secuencia de nucleótidos en una molécula de ácido nucleico, el cual comprende las siguientes etapas: (a) hibridación de moléculas de ácido nucleico con una serie de sondas de distintas secuencias de núcleobases, en que cada sonda posee una masa diferente de todas las demás; (b) separación de las sondas no hibridadas; (c) puesta en contacto de las sondas hibridadas con una matriz que favorece la desorción de las sondas mediante un rayo láser; (d) análisis de las sondas hibridadas y envueltas por la matriz, sobre un soporte de muestras formado por material eléctricamente conductor, en un espectrómetro de masas, y (e) determinación de las moléculas de ácido nucleico que presentan la secuencia, de modo que las posiciones de las sondas en el soporte de muestras permiten una asignacióna la molécula de ácido nucleico con la cual se hibridan.
ES98966608T 1997-12-05 1998-12-04 Procedimiento de identificacion de acidos nucleicos mediante espectrometria de masas laser de desorcion/ionizacion asistidas por matriz. Expired - Lifetime ES2173670T3 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
EP97121471 1997-12-05

Publications (1)

Publication Number Publication Date
ES2173670T3 true ES2173670T3 (es) 2002-10-16

Family

ID=8227757

Family Applications (1)

Application Number Title Priority Date Filing Date
ES98966608T Expired - Lifetime ES2173670T3 (es) 1997-12-05 1998-12-04 Procedimiento de identificacion de acidos nucleicos mediante espectrometria de masas laser de desorcion/ionizacion asistidas por matriz.

Country Status (8)

Country Link
EP (1) EP1036202B1 (es)
JP (1) JP2001526381A (es)
AT (1) ATE217028T1 (es)
CA (1) CA2312052A1 (es)
DE (1) DE59804008D1 (es)
DK (1) DK1036202T3 (es)
ES (1) ES2173670T3 (es)
WO (1) WO1999029898A2 (es)

Families Citing this family (51)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE19938138C2 (de) * 1999-08-16 2003-02-13 November Ag Molekulare Medizin Verfahren und Vorrichtung zur Identifikation einer Biopolymersequenz auf Festkörperoberflächen
DE19957827C2 (de) * 1999-11-25 2003-06-12 Epigenomics Ag Verwendung eines Oligomer-Arrays mit PNA- und/oder DNA-Oligomeren auf einer Oberfläche
WO2001077375A2 (en) * 2000-04-06 2001-10-18 Epigenomics Ag Diagnosis of diseases associated with gene regulation
DE10027120A1 (de) * 2000-05-23 2001-12-06 Epigenomics Ag Probenträger für Massenspektrometer
WO2002002807A2 (en) * 2000-06-30 2002-01-10 Epigenomics Ag Diagnosis of diseases associated with cell signalling
DE10054974A1 (de) * 2000-11-06 2002-06-06 Epigenomics Ag Diagnose von mit Cdk4 assoziierten Krankheiten
DE10061338A1 (de) * 2000-12-06 2002-06-20 Epigenomics Ag Diagnose von mit Angiogenese assoziierten Krankheiten
EP1215199A1 (en) * 2000-12-08 2002-06-19 Sony International (Europe) GmbH Linker molecules for selective metallisation of nucleic acids and their uses
US20040121309A1 (en) 2002-12-06 2004-06-24 Ecker David J. Methods for rapid detection and identification of bioagents in blood, bodily fluids, and bodily tissues
US7226739B2 (en) 2001-03-02 2007-06-05 Isis Pharmaceuticals, Inc Methods for rapid detection and identification of bioagents in epidemiological and forensic investigations
WO2004060278A2 (en) 2002-12-06 2004-07-22 Isis Pharmaceuticals, Inc. Methods for rapid identification of pathogens in humans and animals
US20030027135A1 (en) 2001-03-02 2003-02-06 Ecker David J. Method for rapid detection and identification of bioagents
US7666588B2 (en) 2001-03-02 2010-02-23 Ibis Biosciences, Inc. Methods for rapid forensic analysis of mitochondrial DNA and characterization of mitochondrial DNA heteroplasmy
JP2002372517A (ja) * 2001-05-31 2002-12-26 Inst Of Physical & Chemical Res 質量分析法によるタンパク質の構造解析方法
US7217510B2 (en) 2001-06-26 2007-05-15 Isis Pharmaceuticals, Inc. Methods for providing bacterial bioagent characterizing information
US8073627B2 (en) 2001-06-26 2011-12-06 Ibis Biosciences, Inc. System for indentification of pathogens
EP1488003A1 (en) * 2002-03-25 2004-12-22 Epigenomics AG Method for the analysis of methylation patterns within nucleic acids by means of mass spectrometry
EP1522853A4 (en) * 2002-06-24 2005-10-19 Canon Kk DNA MICROARRAY WITH STANDARD PROBE AND KIT CONTAINING THE MICROARRAY
US7964343B2 (en) 2003-05-13 2011-06-21 Ibis Biosciences, Inc. Method for rapid purification of nucleic acids for subsequent analysis by mass spectrometry by solution capture
US8158354B2 (en) 2003-05-13 2012-04-17 Ibis Biosciences, Inc. Methods for rapid purification of nucleic acids for subsequent analysis by mass spectrometry by solution capture
US8242254B2 (en) 2003-09-11 2012-08-14 Ibis Biosciences, Inc. Compositions for use in identification of bacteria
US8097416B2 (en) 2003-09-11 2012-01-17 Ibis Biosciences, Inc. Methods for identification of sepsis-causing bacteria
US8546082B2 (en) 2003-09-11 2013-10-01 Ibis Biosciences, Inc. Methods for identification of sepsis-causing bacteria
US7666592B2 (en) 2004-02-18 2010-02-23 Ibis Biosciences, Inc. Methods for concurrent identification and quantification of an unknown bioagent
ES2641832T3 (es) 2004-05-24 2017-11-14 Ibis Biosciences, Inc. Espectrometría de masas con filtración de iones selectiva por establecimiento de umbrales digitales
US20050266411A1 (en) 2004-05-25 2005-12-01 Hofstadler Steven A Methods for rapid forensic analysis of mitochondrial DNA
US7811753B2 (en) 2004-07-14 2010-10-12 Ibis Biosciences, Inc. Methods for repairing degraded DNA
WO2006135400A2 (en) 2004-08-24 2006-12-21 Isis Pharmaceuticals, Inc. Methods for rapid identification of recombinant organisms
US8084207B2 (en) 2005-03-03 2011-12-27 Ibis Bioscience, Inc. Compositions for use in identification of papillomavirus
US20060205040A1 (en) 2005-03-03 2006-09-14 Rangarajan Sampath Compositions for use in identification of adventitious viruses
JP2009502137A (ja) 2005-07-21 2009-01-29 アイシス ファーマシューティカルズ インコーポレイティッド 核酸変種の迅速な同定および定量のための方法
EP1775348A1 (en) * 2005-10-17 2007-04-18 Max-Planck-Gesellschaft zur Förderung der Wissenschaften e.V. Method of analyzing nucleic acid with mass spectrometry
EP2010679A2 (en) 2006-04-06 2009-01-07 Ibis Biosciences, Inc. Compositions for the use in identification of fungi
US9149473B2 (en) 2006-09-14 2015-10-06 Ibis Biosciences, Inc. Targeted whole genome amplification method for identification of pathogens
JP4984900B2 (ja) * 2007-01-12 2012-07-25 株式会社島津製作所 ポリマー被覆微粒子を用いる質量分析法
EP2126132B1 (en) 2007-02-23 2013-03-20 Ibis Biosciences, Inc. Methods for rapid foresnsic dna analysis
WO2008151023A2 (en) 2007-06-01 2008-12-11 Ibis Biosciences, Inc. Methods and compositions for multiple displacement amplification of nucleic acids
WO2010033625A1 (en) 2008-09-16 2010-03-25 Ibis Biosciences, Inc. Microplate handling systems and related computer program products and methods
US8148163B2 (en) 2008-09-16 2012-04-03 Ibis Biosciences, Inc. Sample processing units, systems, and related methods
US8550694B2 (en) 2008-09-16 2013-10-08 Ibis Biosciences, Inc. Mixing cartridges, mixing stations, and related kits, systems, and methods
EP2396803A4 (en) 2009-02-12 2016-10-26 Ibis Biosciences Inc IONIZATION PROBE ASSEMBLIES
US9719083B2 (en) 2009-03-08 2017-08-01 Ibis Biosciences, Inc. Bioagent detection methods
WO2010114842A1 (en) 2009-03-30 2010-10-07 Ibis Biosciences, Inc. Bioagent detection systems, devices, and methods
EP2454000A4 (en) 2009-07-17 2016-08-10 Ibis Biosciences Inc SYSTEMS FOR IDENTIFYING BIOLOGICAL SUBSTANCES
US8950604B2 (en) 2009-07-17 2015-02-10 Ibis Biosciences, Inc. Lift and mount apparatus
WO2011014811A1 (en) 2009-07-31 2011-02-03 Ibis Biosciences, Inc. Capture primers and capture sequence linked solid supports for molecular diagnostic tests
EP3098325A1 (en) 2009-08-06 2016-11-30 Ibis Biosciences, Inc. Non-mass determined base compositions for nucleic acid detection
EP2488656B1 (en) 2009-10-15 2015-06-03 Ibis Biosciences, Inc. Multiple displacement amplification
US9758840B2 (en) 2010-03-14 2017-09-12 Ibis Biosciences, Inc. Parasite detection via endosymbiont detection
KR20120090473A (ko) * 2011-02-08 2012-08-17 주식회사 아스타 말디 이미징용 매트릭스 용액 및 이를 이용한 생체 분자의 말디 이미징의 측정 방법
NL2022038B1 (en) * 2018-11-21 2020-06-05 Biosparq B V Method for analysing an analyte sample and matrix material therefore

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2074214C (en) * 1991-07-23 2000-02-22 Will Bloch Improvements in the in situ pcr
GB9315847D0 (en) * 1993-07-30 1993-09-15 Isis Innovation Tag reagent and assay method
GB9504598D0 (en) * 1995-03-03 1995-04-26 Imp Cancer Res Tech Method of nucleic acid analysis
JP3884087B2 (ja) * 1997-01-15 2007-02-21 イクスツィリオン ゲゼルシャフト ミット ベシュレンクテル ハフツング ウント コンパニー コマンディトゲゼルシャフト 質量標識結合ハイブリダイゼーションプローブ

Also Published As

Publication number Publication date
WO1999029898A3 (de) 1999-10-28
ATE217028T1 (de) 2002-05-15
DK1036202T3 (da) 2002-08-12
DE59804008D1 (de) 2002-06-06
EP1036202B1 (de) 2002-05-02
JP2001526381A (ja) 2001-12-18
WO1999029898A2 (de) 1999-06-17
EP1036202A2 (de) 2000-09-20
CA2312052A1 (en) 1999-06-17

Similar Documents

Publication Publication Date Title
ES2173670T3 (es) Procedimiento de identificacion de acidos nucleicos mediante espectrometria de masas laser de desorcion/ionizacion asistidas por matriz.
NZ336769A (en) Method of determining hybridization of a probe to a target nucleic acid
França et al. A review of DNA sequencing techniques
Little et al. MALDI on a chip: analysis of arrays of low-femtomole to subfemtomole quantities of synthetic oligonucleotides and DNA diagnostic products dispensed by a piezoelectric pipet
US6750022B2 (en) Mutation analysis by PCR and mass spectrometry
US5605798A (en) DNA diagnostic based on mass spectrometry
US6428955B1 (en) DNA diagnostics based on mass spectrometry
Köster et al. A strategy for rapid and efficient DNA sequencing by mass spectrometry
AU732167B2 (en) Process for detecting nucleic acids by mass determination
EP1373561B1 (en) Use of mass-matched nucleotides in the analysis of oligonucleotide mixtures and in highly multiplexed nucleic acid sequencing
US7198893B1 (en) DNA diagnostics based on mass spectrometry
JP5382802B2 (ja) 質量分析法を用いた生体分子の検出および定量
US5807677A (en) Method for direct identification of few nucleic acid strands
US5780232A (en) DNA sequencing, mapping, and diagnostic processes using hybridization and stable isotope labels of DNA
KR19990081923A (ko) 비형광 표지를 이용하여 리간드쌍의 결합을 검출하기 위한방법 및 조성물
NO20004962D0 (no) FremgangsmÕte ved pÕvisning av nukleinsyrer
US20040019005A1 (en) Methods for parallel measurement of genetic variations
Banoub et al. Mass spectrometry of nucleosides and nucleic acids
US20040058349A1 (en) Methods for identifying nucleotides at defined positions in target nucleic acids
HUP0003261A2 (hu) Több funkció- vagy mátrixelemen belül, továbbá ezek alkalmazásai
US6770443B2 (en) Method and apparatus for mass spectrometric genotyping
Wunschel et al. Electrospray ionization Fourier transform ion
Allman et al. Laser Desorption Mass Spectrometry for High Throughput DNA Analysis and Its Applications
Isola et al. Matrix‐assisted laser desorption/ionization detection of polymerase chain reaction products by utilizing the 5′‐3′ exonuclease activity of Thermus aquaticus DNA polymerase
Benner et al. DNA base-pair substitutions detected in double-stranded DNA with matrix-assisted laser desorption/ionization time-of-flight mass spectrometry

Legal Events

Date Code Title Description
FG2A Definitive protection

Ref document number: 1036202

Country of ref document: ES