ES2174521T3 - Instalacion de deteccion de fluorescencia para la determinacion de parametros de vegetacion importantes. - Google Patents

Instalacion de deteccion de fluorescencia para la determinacion de parametros de vegetacion importantes.

Info

Publication number
ES2174521T3
ES2174521T3 ES98961105T ES98961105T ES2174521T3 ES 2174521 T3 ES2174521 T3 ES 2174521T3 ES 98961105 T ES98961105 T ES 98961105T ES 98961105 T ES98961105 T ES 98961105T ES 2174521 T3 ES2174521 T3 ES 2174521T3
Authority
ES
Spain
Prior art keywords
signal
fluorescence
excitation
laser
electronic
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
ES98961105T
Other languages
English (en)
Inventor
Wilhelm Ludeker
Kurt Gunther
Hans-Gunter Dahn
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Deutsches Zentrum fuer Luft und Raumfahrt eV
Original Assignee
Deutsches Zentrum fuer Luft und Raumfahrt eV
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Deutsches Zentrum fuer Luft und Raumfahrt eV filed Critical Deutsches Zentrum fuer Luft und Raumfahrt eV
Application granted granted Critical
Publication of ES2174521T3 publication Critical patent/ES2174521T3/es
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

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Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/6486—Measuring fluorescence of biological material, e.g. DNA, RNA, cells
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/645—Specially adapted constructive features of fluorimeters
    • G01N21/6456—Spatial resolved fluorescence measurements; Imaging
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N2021/635—Photosynthetic material analysis, e.g. chrorophyll
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/84—Systems specially adapted for particular applications
    • G01N2021/8466—Investigation of vegetal material, e.g. leaves, plants, fruits
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2201/00—Features of devices classified in G01N21/00
    • G01N2201/06—Illumination; Optics
    • G01N2201/061—Sources
    • G01N2201/06113—Coherent sources; lasers
    • G01N2201/0612—Laser diodes

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biochemistry (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Physics & Mathematics (AREA)
  • Chemical & Material Sciences (AREA)
  • Analytical Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Engineering & Computer Science (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)

Abstract

Instalación de detección de fluorescencia para la detección de parámetros de vegetación relevantes, que comprende una fuente de excitación (1) que consiste en un dispositivo láser de baja potencia con una longitud de onda de excitación en la región espectral del rojo, un dispositivo óptico (2) que forma un haz de luz, un divisor de haz dicroico (5), un sistema básico detector de fluorescencia (3) que incluye un dispositivo óptico de entrada (4) que recibe la emisión de fluorescencia a través de dicho divisor de haz dicroico (5) y un filtro de interferencias (10) que bloquea la señal de retrodispersión elástica, un dispositivo electrónico de detección (7) para detectar una señal de fluorescencia, y un dispositivo electrónico disparador y temporizador (6), caracterizada porque el citado dispositivo láser de baja potencia previsto en la fuente de excitación (1) es un dispositivo láser pulsatorio de alta repetición con longitudde impulsos de algunos nanosegundos y una longitud de onda de excitación preferida en la región espectral del rojo de preferentemente 670 nm, porque el citado divisor de haz dicroico (5) acopla el haz de excitación formado coaxialmente al eje óptico (9) de una óptica receptora y dirigiendo este haz formado sin guiado óptico de ondas hasta un blanco de vegetación sujeto a ser investigado, porque dicho sistema básico detector de fluorescencia (3) forma una imagen del punto de excitación en el área sensible del detector, porque el citado dispositivo electrónico de detección (7) opera en el régimen de repetición de doble impulso de dicha fuente láser de excitación (1) y toma muestras de la señal de fluorescencia activa sincrónicamente con la emisión de láser por una parte y por otra parte con la señal de fondo pasiva con un retardo fijo del orden de microsegundos antes o después de la señal activa, registrando las señales por medio de un circuito de muestra rápida y mantenimiento acoplado a un convertidor analógico a digital que permite un tratamiento de señal digital, porque dicho dispositivo electrónico de detección (7) comprende además medios para determinar la señal de fluorescencia pura sustrayendo la señal de fondo de la señal de fluorescencia activa, electrónicamente o en una operación de tratamiento posterior, y porque el citado dispositivo electrónico disparador y temporizador (6) sincroniza los impulsos de láser con los intervalos de muestra de dicho dispositivo electrónico de detección (7).
ES98961105T 1998-10-28 1998-10-28 Instalacion de deteccion de fluorescencia para la determinacion de parametros de vegetacion importantes. Expired - Lifetime ES2174521T3 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
PCT/EP1998/006815 WO2000025114A1 (en) 1998-10-28 1998-10-28 Fluorescence detection assembly for determination of significant vegetation parameters

Publications (1)

Publication Number Publication Date
ES2174521T3 true ES2174521T3 (es) 2002-11-01

Family

ID=8167113

Family Applications (1)

Application Number Title Priority Date Filing Date
ES98961105T Expired - Lifetime ES2174521T3 (es) 1998-10-28 1998-10-28 Instalacion de deteccion de fluorescencia para la determinacion de parametros de vegetacion importantes.

Country Status (9)

Country Link
US (1) US6563122B1 (es)
EP (1) EP1125111B1 (es)
AT (1) ATE218705T1 (es)
AU (1) AU752897B2 (es)
CA (1) CA2348594C (es)
DE (1) DE69805862T2 (es)
ES (1) ES2174521T3 (es)
UA (1) UA66892C2 (es)
WO (1) WO2000025114A1 (es)

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CN113092428B (zh) * 2021-04-02 2023-07-25 安图实验仪器(郑州)有限公司 一种多重荧光检测方法、装置、设备和系统

Also Published As

Publication number Publication date
CA2348594A1 (en) 2000-05-04
EP1125111B1 (en) 2002-06-05
EP1125111A1 (en) 2001-08-22
DE69805862D1 (de) 2002-07-11
CA2348594C (en) 2006-12-12
DE69805862T2 (de) 2003-01-16
ATE218705T1 (de) 2002-06-15
UA66892C2 (uk) 2004-06-15
WO2000025114A1 (en) 2000-05-04
AU752897B2 (en) 2002-10-03
AU1664699A (en) 2000-05-15
US6563122B1 (en) 2003-05-13

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