ES2177536T3 - G-csf exento de metionina, procedente de procariotas. - Google Patents

G-csf exento de metionina, procedente de procariotas.

Info

Publication number
ES2177536T3
ES2177536T3 ES93121015T ES93121015T ES2177536T3 ES 2177536 T3 ES2177536 T3 ES 2177536T3 ES 93121015 T ES93121015 T ES 93121015T ES 93121015 T ES93121015 T ES 93121015T ES 2177536 T3 ES2177536 T3 ES 2177536T3
Authority
ES
Spain
Prior art keywords
protein
procariots
metionin
exempt
csf
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
ES93121015T
Other languages
English (en)
Inventor
Gunter Dr Schumacher
Carola Dr Dony
Thomas F Meyer
Johannes Pohlner
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Max Planck Gesellschaft zur Foerderung der Wissenschaften eV
Original Assignee
Max Planck Gesellschaft zur Foerderung der Wissenschaften eV
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from DE4015922A external-priority patent/DE4015922A1/de
Priority claimed from DE4039415A external-priority patent/DE4039415A1/de
Application filed by Max Planck Gesellschaft zur Foerderung der Wissenschaften eV filed Critical Max Planck Gesellschaft zur Foerderung der Wissenschaften eV
Application granted granted Critical
Publication of ES2177536T3 publication Critical patent/ES2177536T3/es
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/52—Cytokines; Lymphokines; Interferons
    • C07K14/54—Interleukins [IL]
    • C07K14/5403—IL-3
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P37/00—Drugs for immunological or allergic disorders
    • A61P37/02—Immunomodulators
    • A61P37/04—Immunostimulants
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/28—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Vibrionaceae (F)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/52—Cytokines; Lymphokines; Interferons
    • C07K14/53—Colony-stimulating factor [CSF]
    • C07K14/535—Granulocyte CSF; Granulocyte-macrophage CSF
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/52—Cytokines; Lymphokines; Interferons
    • C07K14/54—Interleukins [IL]
    • C07K14/5409—IL-5
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/52—Cytokines; Lymphokines; Interferons
    • C07K14/54—Interleukins [IL]
    • C07K14/55—IL-2
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/705—Receptors; Cell surface antigens; Cell surface determinants
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K19/00—Hybrid peptides, i.e. peptides covalently bound to nucleic acids, or non-covalently bound protein-protein complexes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/62—DNA sequences coding for fusion proteins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/52—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/06—Preparation of peptides or proteins produced by the hydrolysis of a peptide bond, e.g. hydrolysate products
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Medicinal Chemistry (AREA)
  • Biophysics (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Biomedical Technology (AREA)
  • Toxicology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Immunology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Cell Biology (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Animal Behavior & Ethology (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

PARA DESDOBLAMIENTO ENZIMATICO DE PROTEINAS FUSIONADAS Y OBTENCION DE LAS PORCIONES DESEADAS DE ESTA PROTEINA FUSIONADA SE MODIFICAN (1) CON MEDIOS TECNICOS DE GENE UNA REGION DE TRANSICION, EN LA QUE LA SEGUNDA PORCION DE LA PROTEINA FUSIONADA ESTA UNIDA DE TAL MODO, QUE EN ESTA REGION DE TRANSICION SE GENERA AL MENOS UNA POSICION DE RECONOCIMIENTO DE LA PROTEASA IGA CON LA SECUENCIA DE AMINOACIDOS YPRO.!.X-PRO, DONDE X ES UN AMINOACIDO CUALQUIERA E Y PUEDE SER UNO O MULTIPLES AMINOACIDOS HABITUALES, (2) DESDOBLAMIENTO DE LA PROTEINA DE FUSION QUE RESULTA DE LA ETAPA (1) POR MEDIO DE PROTEASA IGA CON LA POSICION CARACTERISTICA .!. DEL LUGAR DE RECONOCIMIENTO Y (3) OBTENCION DESPUES DEL DESDOBLAMIENTO DE UNA O MULTIPLES PORCIONES DESEADAS DE LA PROTEINA FUSIONADA.
ES93121015T 1990-02-03 1991-02-01 G-csf exento de metionina, procedente de procariotas. Expired - Lifetime ES2177536T3 (es)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
DE4003149 1990-02-03
DE4015922A DE4015922A1 (de) 1990-05-17 1990-05-17 Verfahren zur enzymatischen prozessierung von proteinen unter verwendung von iga-proteasen (igase)
DE4015921 1990-05-17
DE4039415A DE4039415A1 (de) 1990-02-03 1990-12-10 Verfahren zur herstellung rekombinanter proteine ohne n-terminalen methioninrest

Publications (1)

Publication Number Publication Date
ES2177536T3 true ES2177536T3 (es) 2002-12-16

Family

ID=27434868

Family Applications (2)

Application Number Title Priority Date Filing Date
ES91902956T Expired - Lifetime ES2076521T3 (es) 1990-02-03 1991-02-01 Procedimiento para la disociacion enzimatica de proteinas recombinantes mediando utilizacion de proteasa de iga.
ES93121015T Expired - Lifetime ES2177536T3 (es) 1990-02-03 1991-02-01 G-csf exento de metionina, procedente de procariotas.

Family Applications Before (1)

Application Number Title Priority Date Filing Date
ES91902956T Expired - Lifetime ES2076521T3 (es) 1990-02-03 1991-02-01 Procedimiento para la disociacion enzimatica de proteinas recombinantes mediando utilizacion de proteasa de iga.

Country Status (19)

Country Link
US (1) US5427927A (es)
EP (2) EP0513073B1 (es)
JP (2) JPH0789952B2 (es)
KR (1) KR960011919B1 (es)
AT (2) ATE124456T1 (es)
AU (1) AU638309B2 (es)
CA (1) CA2074943C (es)
CZ (1) CZ284774B6 (es)
DK (1) DK0513073T3 (es)
ES (2) ES2076521T3 (es)
FI (1) FI109810B (es)
HU (1) HU217103B (es)
IE (1) IE64938B1 (es)
IL (1) IL97119A0 (es)
LV (1) LV10309B (es)
NO (1) NO311142B1 (es)
NZ (1) NZ236819A (es)
PT (1) PT96658B (es)
WO (1) WO1991011520A1 (es)

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US5536495A (en) * 1994-04-15 1996-07-16 Foster; Preston F. Use of G-CSF to reduce acute rejection
WO1996009395A2 (de) * 1994-09-21 1996-03-28 MAX-PLANCK-Gesellschaft zur Förderung der Wissenschaften e.V. Arzneimittel zur prophylaxe und behandlung von und diagnostika zur erkennung von autoimmunkrankheiten und viralen erkrankungen
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US20030190644A1 (en) * 1999-10-13 2003-10-09 Andreas Braun Methods for generating databases and databases for identifying polymorphic genetic markers
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ATE544463T1 (de) 2004-11-05 2012-02-15 Univ Northwestern Verwendung von scf und g-scf bei der behandlung von hirnischämie und neurologischen störungen
UA95446C2 (ru) 2005-05-04 2011-08-10 Іллюміджен Байосайєнсіз, Інк. Мутаци в генах oas1
CN101484469B (zh) * 2006-08-31 2012-12-12 弗·哈夫曼-拉罗切有限公司 生产胰岛素样生长因子-ⅰ的方法
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CR20210429A (es) 2015-06-24 2021-09-24 Hoffmann La Roche ANTICUERPOS ANTI-RECEPTOR DE TRANSFERRINA CON AFINIDAD DISEÑADA (Divisional 2017-0562)
AR106189A1 (es) 2015-10-02 2017-12-20 Hoffmann La Roche ANTICUERPOS BIESPECÍFICOS CONTRA EL A-b HUMANO Y EL RECEPTOR DE TRANSFERRINA HUMANO Y MÉTODOS DE USO
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JP7736395B1 (ja) * 2024-02-29 2025-09-09 積水メディカル株式会社 免疫学的測定方法、免疫学的測定用試薬、免疫学的測定用検体前処理液、免疫学的測定用試薬キット、及び非特異反応抑制剤

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Also Published As

Publication number Publication date
EP0513073A1 (de) 1992-11-19
IL97119A0 (en) 1992-03-29
FI923463A0 (fi) 1992-07-31
ES2076521T3 (es) 1995-11-01
JPH07278195A (ja) 1995-10-24
EP0513073B1 (de) 1995-06-28
ATE124456T1 (de) 1995-07-15
EP0602688B1 (de) 2002-06-19
IE64938B1 (en) 1995-09-20
NO923010D0 (no) 1992-07-30
HU217103B (hu) 1999-11-29
FI109810B (fi) 2002-10-15
JP2766621B2 (ja) 1998-06-18
EP0602688A1 (en) 1994-06-22
NZ236819A (en) 1993-07-27
PT96658A (pt) 1991-10-31
LV10309A (lv) 1994-10-20
JPH0789952B2 (ja) 1995-10-04
CA2074943C (en) 1999-12-07
ATE219518T1 (de) 2002-07-15
FI923463L (fi) 1992-07-31
CZ284774B6 (cs) 1999-02-17
DK0513073T3 (da) 1995-10-30
AU7149691A (en) 1991-08-21
AU638309B2 (en) 1993-06-24
NO923010L (no) 1992-09-14
HU9202511D0 (en) 1992-10-28
US5427927A (en) 1995-06-27
KR960011919B1 (ko) 1996-09-04
JPH05501360A (ja) 1993-03-18
NO311142B1 (no) 2001-10-15
WO1991011520A1 (de) 1991-08-08
PT96658B (pt) 1998-09-30
LV10309B (en) 1995-12-20
HUT63195A (en) 1993-07-28
CA2074943A1 (en) 1991-08-04
CS9100241A2 (en) 1991-08-13
IE910358A1 (en) 1991-08-14

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