ES2177602T3 - Metodo para controlar la expresion de genes en acido lactico. - Google Patents

Metodo para controlar la expresion de genes en acido lactico.

Info

Publication number
ES2177602T3
ES2177602T3 ES95203153T ES95203153T ES2177602T3 ES 2177602 T3 ES2177602 T3 ES 2177602T3 ES 95203153 T ES95203153 T ES 95203153T ES 95203153 T ES95203153 T ES 95203153T ES 2177602 T3 ES2177602 T3 ES 2177602T3
Authority
ES
Spain
Prior art keywords
genes
expression
promoter
dna fragment
refers
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
ES95203153T
Other languages
English (en)
Inventor
Oscar Paul Kuipers
Vos Willem Meindert De
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Stichting Nederlands Instituut Voor Zuivelonderzoek (Nizo)
Original Assignee
Stichting Nederlands Instituut Voor Zuivelonderzoek (Nizo)
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from NL9401935A external-priority patent/NL9401935A/nl
Application filed by Stichting Nederlands Instituut Voor Zuivelonderzoek (Nizo) filed Critical Stichting Nederlands Instituut Voor Zuivelonderzoek (Nizo)
Application granted granted Critical
Publication of ES2177602T3 publication Critical patent/ES2177602T3/es
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01031—Beta-glucuronidase (3.2.1.31)
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23C—DAIRY PRODUCTS, e.g. MILK, BUTTER OR CHEESE; MILK OR CHEESE SUBSTITUTES; PREPARATION THEREOF
    • A23C9/00—Milk preparations; Milk powder or milk powder preparations
    • A23C9/12—Fermented milk preparations; Treatment using microorganisms or enzymes
    • A23C9/123—Fermented milk preparations; Treatment using microorganisms or enzymes using only microorganisms of the genus lactobacteriaceae; Yoghurt
    • A23C9/1238—Fermented milk preparations; Treatment using microorganisms or enzymes using only microorganisms of the genus lactobacteriaceae; Yoghurt using specific L. bulgaricus or S. thermophilus microorganisms; using entrapped or encapsulated yoghurt bacteria; Physical or chemical treatment of L. bulgaricus or S. thermophilus cultures; Fermentation only with L. bulgaricus or only with S. thermophilus
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23C—DAIRY PRODUCTS, e.g. MILK, BUTTER OR CHEESE; MILK OR CHEESE SUBSTITUTES; PREPARATION THEREOF
    • A23C19/00—Cheese; Cheese preparations; Making thereof
    • A23C19/02—Making cheese curd
    • A23C19/032—Making cheese curd characterised by the use of specific microorganisms, or enzymes of microbial origin
    • A23C19/0323—Making cheese curd characterised by the use of specific microorganisms, or enzymes of microbial origin using only lactic acid bacteria, e.g. Pediococcus and Leuconostoc species; Bifidobacteria; Microbial starters in general
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • C12N15/746—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for lactic acid bacteria (Streptococcus; Lactococcus; Lactobacillus; Pediococcus; Enterococcus; Leuconostoc; Propionibacterium; Bifidobacterium; Sporolactobacillus)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2434—Glucanases acting on beta-1,4-glucosidic bonds
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23C—DAIRY PRODUCTS, e.g. MILK, BUTTER OR CHEESE; MILK OR CHEESE SUBSTITUTES; PREPARATION THEREOF
    • A23C2220/00—Biochemical treatment
    • A23C2220/20—Treatment with microorganisms
    • A23C2220/202—Genetic engineering of microorganisms used in dairy technology

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Polymers & Plastics (AREA)
  • Food Science & Technology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Dairy Products (AREA)
  • Coloring Foods And Improving Nutritive Qualities (AREA)
  • Peptides Or Proteins (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)

Abstract

LA INVENCION SE REFIERE A UN METODO PARA LA EXPRESION CONTROLADA, EN UNA BACTERIA DEL ACIDO LACTICO, DE UN FRAGMENTO DE DNA QUE CONTIENE UNO O MAS GENES QUE CODIFICAN UNA CARACTERISTICA DESEADA, QUE SE CARACTERIZA EN QUE: - EL FRAGMENTO DE DNA ESTA BAJO EL CONTROL DE UN PROMOTOR PARA UN GEN MICROBIANO QUE CODIFICA UN PEPTIDO ANTIMICROBIANO, Y - EL GEN/LOS GENES SON PUESTOS EN EXPRESION SOBRE EL FRAGMENTO DE DNA MEDIANTE LA ADICION DE UN FACTOR DE INDUCCION ADECUADO PARA LA ACTIVACION DE LA TRANSCRIPCION. EL PROMOTOR ES PREFERIBLEMENTE EL PROMOTOR "NIS/A" DEL "LACTOCOCCUS LACTIS". EL INDUCTOR ES PREFERIBLEMENTE ACEPTABLE PARA PRODUCTOS ALIMENTICIOS, Y MAS PREFERENTEMENTE ES NISINA O UN DERIVADO DE LA MISMA. LA INVENCION TAMBIEN SE REFIERE A UN METODO PARA LA PRODUCCION DE PROTEINAS O RNA, ASI COMO A UN METODO PARA LA PRODUCCION DE PRODUCTOS DERIVADOS DE LA LECHE UTILIZANDO EL SISTEMA DE EXPRESION DE ACUERDO CON LA INVENCION. LA INVENCION FINALMENTE SE REFIERE A UNA BACTERIA DEL ACIDO LACTICOY A VECTORES DE EXPRESION PARA SU USO EN EL METODO DE ACUERDO CON LA INVENCION.
ES95203153T 1994-11-18 1995-11-17 Metodo para controlar la expresion de genes en acido lactico. Expired - Lifetime ES2177602T3 (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
NL9401934 1994-11-18
NL9401935A NL9401935A (nl) 1994-11-18 1994-11-18 Werkwijze voor het regelen van de gen-expressie in melkzuurbacteriën.

Publications (1)

Publication Number Publication Date
ES2177602T3 true ES2177602T3 (es) 2002-12-16

Family

ID=26647280

Family Applications (1)

Application Number Title Priority Date Filing Date
ES95203153T Expired - Lifetime ES2177602T3 (es) 1994-11-18 1995-11-17 Metodo para controlar la expresion de genes en acido lactico.

Country Status (5)

Country Link
US (1) US5914248A (es)
EP (2) EP0712935B1 (es)
DE (1) DE69527126T2 (es)
DK (1) DK0712935T3 (es)
ES (1) ES2177602T3 (es)

Families Citing this family (17)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
NO954575D0 (no) * 1995-11-13 1995-11-13 Vincent G H Eijsink Ekspresjonssystem i mikroorganisme og dets anvendelse til å uttrykke heterologe og homologe proteiner
DE69716622T2 (de) * 1996-09-06 2003-07-10 Bioteknologisk Institut, Hoersholm Milchsäurebakterielles regulierbares expressionssystem
US6613364B2 (en) 1999-08-31 2003-09-02 Kraft Foods Holdings, Inc. Stabilization of cooked meat and meat-vegetable compositions using whey from nisin-producing cultures and product thereof
US6833260B1 (en) 1999-10-08 2004-12-21 Protein Scientific, Inc. Lactose hydrolysis
US6797308B2 (en) 2001-02-08 2004-09-28 Kraft Foods Holdings, Inc. Stabilization of cooked pasta compositions using whey from nisin-producing cultures
US7186426B2 (en) 2001-02-23 2007-03-06 Kraft Foods Holdings, Inc. Prevention of lactic acid bacteria spoilage of beer through use of bacteriocin-containing fermented wort
AU2002314626B2 (en) 2001-06-11 2008-01-03 Applied Nanosystems B.V. Methods for binding AcmA-type protein anchor fusions to cell-wall material of micro-organisms
EP1269993A1 (en) * 2001-06-21 2003-01-02 Applied NanoSystems B.V. Delivery of small hydrophilic molecules packaged into lipid vesicles
US6861236B2 (en) * 2002-05-24 2005-03-01 Applied Nanosystems B.V. Export and modification of (poly)peptides in the lantibiotic way
US20070231833A1 (en) * 2005-05-23 2007-10-04 Arcidiacono Steven M Labeled antimicrobial peptides and method of using the same to detect microorganisms of interest
AU2006282722A1 (en) * 2005-08-26 2007-03-01 Cerenis Therapeutics Holdings S.A. Compositions and methods for producing apolipoprotein gene products in lactic acid bacteria
US20140220177A1 (en) * 2011-04-29 2014-08-07 Compagnie Gervais Danone Use of Nisin Resistant Mutant Strains of Lactobacilli for Reducing the Post Acidification in Food Products
WO2014031842A1 (en) 2012-08-22 2014-02-27 Kraft Foods Group Brands Llc Processed cheese with cultured dairy components and method of manufacturing
EP2897638A1 (en) 2012-09-24 2015-07-29 Montana State University-Bozeman Recombinant lactococcus lactis expressing escherichia coli colonization factor antigen i (cfa/i) fimbriae and their methods of use
ES2778727T3 (es) 2014-08-25 2020-08-11 Geneweave Biosciences Inc Partículas de transducción no replicativas y sistemas indicadores basados en partículas de transducción
US11008602B2 (en) 2017-12-20 2021-05-18 Roche Molecular Systems, Inc. Non-replicative transduction particles and transduction particle-based reporter systems
EP4568490A1 (en) * 2022-08-12 2025-06-18 Chr. Hansen A/S <smallcaps/>? ? ?lactococcus lactis? ? ? ? ?controlling the flavour potential and phage risk ofin cheddar cheese

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU6128086A (en) * 1985-07-01 1987-01-30 Trustees Of Columbia University In The City Of New York, The Plasmids which include promoters for bacteriocins adapted for expression of foreign polypeptides in escherichia coli
NL8801529A (nl) * 1988-06-15 1990-01-02 Nl Zuivelonderzoek Inst Werkwijze voor het selecteren en stabiel handhaven van recombinant dna in melkzuurbacterien.
WO1992004451A1 (en) * 1990-08-30 1992-03-19 Genesit Oy Promoter probe vectors, able to replicate in e.coli, b.subtilis, lactococci and lactobacillus as well as uses thereof
GB9207267D0 (en) * 1992-04-02 1992-05-13 Agricultural & Food Res Nisins
AU4329793A (en) * 1992-06-30 1994-01-24 Viagen Oy (lactobacillus) expression system using surface protein gene sequences

Also Published As

Publication number Publication date
DE69527126D1 (de) 2002-07-25
EP0712935A2 (en) 1996-05-22
DK0712935T3 (da) 2002-07-15
EP0712935A3 (en) 1997-11-26
DE69527126T2 (de) 2002-12-12
EP1162271A2 (en) 2001-12-12
EP1162271A3 (en) 2003-01-15
US5914248A (en) 1999-06-22
EP0712935B1 (en) 2002-06-19

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