ES2178101T3 - Genes de virulencia en la region vgc2 de salmonella. - Google Patents

Genes de virulencia en la region vgc2 de salmonella.

Info

Publication number
ES2178101T3
ES2178101T3 ES98201907T ES98201907T ES2178101T3 ES 2178101 T3 ES2178101 T3 ES 2178101T3 ES 98201907 T ES98201907 T ES 98201907T ES 98201907 T ES98201907 T ES 98201907T ES 2178101 T3 ES2178101 T3 ES 2178101T3
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ES
Spain
Prior art keywords
microorganism
gene
microorganisms
mutant
marker
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
ES98201907T
Other languages
English (en)
Inventor
David William Holden
Jacqueline Elizabeth Shea
Michael Hensel
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Emergent Product Development UK Ltd
Ip2ipo Innovations Ltd
Original Assignee
Microscience Ltd
Imperial College Innovations Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from GBGB9424921.6A external-priority patent/GB9424921D0/en
Priority claimed from GBGB9501881.8A external-priority patent/GB9501881D0/en
Priority claimed from GBGB9509239.1A external-priority patent/GB9509239D0/en
Application filed by Microscience Ltd, Imperial College Innovations Ltd filed Critical Microscience Ltd
Application granted granted Critical
Publication of ES2178101T3 publication Critical patent/ES2178101T3/es
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
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    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
    • C12Q1/04—Determining presence or kind of microorganism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor
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    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/21—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Pseudomonadaceae (F)
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
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    • A61P31/04—Antibacterial agents
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    • A61P31/10—Antimycotics
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    • C07K14/24—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Enterobacteriaceae (F), e.g. Citrobacter, Serratia, Proteus, Providencia, Morganella, Yersinia
    • C07K14/255—Salmonella (G)
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    • C07K14/3156—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Streptococcus (G), e.g. Enterococci from Streptococcus pneumoniae (Pneumococcus)
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    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
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Abstract

UN MICROORGANISMO CON ADAPTACION REDUCIDA A UN ENTORNO PARTICULAR, IDENTIFICADO CON UN METODO QUE INCLUYE LOS PASOS DE: (1) OBTENER MULTIPLES MICROORGANISMOS CADA UNO DE LOS CUALES ES INDEPENDIENTEMENTE MUTADO MEDIANTE INACTIVACION POR INSERCION DE UN GEN CON UN ACIDO NUCLEICO QUE INCLUYE UNA SECUENCIA DE MARCADOR UNICA DE MODO QUE CADA MUTANTE CONTIENE UNA SECUENCIA DE MARCADOR DIFERENTE, O CLONES DE DICHO MICROORGANISMO; (2) OBTENER DE FORMA INDIVIDUAL UNA MUESTRA ALMACENADA DE CADA MUTANTE PRODUCIDO EN EL PASO (1) Y OBTENER DE FORMA INDIVIDUAL ACIDOS NUCLEICOS ALMACENADOS QUE CONTIENEN LA SECUENCIA DE MARCADOR UNICA DE CADA MUTANTE INDIVIDUAL; (3) INTRODUCIR MULTIPLES MUTANTES PRODUCIDOS MEDIANTE EL PASO (1) EN DICHO ENTORNO PARTICULAR, Y PERMITIR QUE LOS MICROORGANISMOS CAPACES DE CRECER EN DICHO ENTORNO LO HAGAN; (4) EXTRAER MICROORGANISMOS DE DICHO ENTORNO O UNA PARTE SELECCIONADA DEL MISMO, Y AISLAR EL ACIDO NUCLEICO DE LOS MICROORGANISMOS EXTRAIDOS; (5) COMPARAR LAS SECUENCIAS DE MARCADOR DE LOS ACIDOS NUCLEICOS AISLADOS EN EL PASO (4) CON LA SECUENCIA DE MARCADOR UNICA DE CADA MUTANTE INDIVIDUAL ALMACENADO EN EL PASO (2); Y (6) SELECCIONAR UN MUTANTE INDIVIDUAL QUE NO CONTIENE NINGUNA DE LAS SECUENCIAS DE MARCADOR AISLADAS EN EL PASO (4). ADEMAS, LA INVENCION SE REFIERE A: UN GEN AISLADO DEL MICROORGANISMO, UNA VACUNA QUE INCLUYE EL MICROORGANISMO, UN POLIPEPTIDO CODIFICADO POR EL GEN, UN METODO PARA IDENTIFICAR COMPUESTOS QUE INTERFIEREN CON LA FUNCION DEL GEN, P.EJ: POR ANTISENTIDO. EL MICROORGANISMO SE EJEMPLIFICA CON SALMONELLA TYPHIMURIUM Y SU GEN VGC2.
ES98201907T 1994-12-09 1995-12-11 Genes de virulencia en la region vgc2 de salmonella. Expired - Lifetime ES2178101T3 (es)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
GBGB9424921.6A GB9424921D0 (en) 1994-12-09 1994-12-09 Identification of genes
GBGB9501881.8A GB9501881D0 (en) 1995-01-31 1995-01-31 Identification of genes
GBGB9509239.1A GB9509239D0 (en) 1995-05-05 1995-05-05 Identification of genes

Publications (1)

Publication Number Publication Date
ES2178101T3 true ES2178101T3 (es) 2002-12-16

Family

ID=27267507

Family Applications (2)

Application Number Title Priority Date Filing Date
ES98201907T Expired - Lifetime ES2178101T3 (es) 1994-12-09 1995-12-11 Genes de virulencia en la region vgc2 de salmonella.
ES95939362T Expired - Lifetime ES2126332T3 (es) 1994-12-09 1995-12-11 Metodo para la identificacion de genes implicados en la adaptacion de un microorganismo a su entorno.

Family Applications After (1)

Application Number Title Priority Date Filing Date
ES95939362T Expired - Lifetime ES2126332T3 (es) 1994-12-09 1995-12-11 Metodo para la identificacion de genes implicados en la adaptacion de un microorganismo a su entorno.

Country Status (20)

Country Link
US (7) US5876931A (es)
EP (3) EP1285960A3 (es)
JP (3) JP3522285B2 (es)
KR (4) KR100551104B1 (es)
CN (3) CN1912141B (es)
AT (2) ATE215986T1 (es)
AU (1) AU711524C (es)
CA (2) CA2206515C (es)
CZ (1) CZ296981B6 (es)
DE (3) DE69526377T2 (es)
DK (2) DK0796341T3 (es)
ES (2) ES2178101T3 (es)
FI (1) FI121601B (es)
HU (1) HUT76975A (es)
NO (2) NO320492B1 (es)
NZ (3) NZ511170A (es)
PT (1) PT889120E (es)
RU (1) RU2370541C2 (es)
SG (4) SG56084A1 (es)
WO (1) WO1996017951A2 (es)

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WO1999037759A2 (en) * 1998-01-22 1999-07-29 Vrije Universiteit Brussel Live attenuated salmonella vaccine
EP0943681A1 (en) * 1998-01-22 1999-09-22 Vrije Universiteit Brussel Live attenuated salmonella vaccine
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WO1999047643A1 (en) * 1998-03-18 1999-09-23 Quark Biotech, Inc. Selection subtraction approach to gene identification
WO1999059630A1 (en) * 1998-05-15 1999-11-25 University Of California Los Angeles Type iii bordetella secretion system
DE69939264D1 (de) * 1998-09-04 2008-09-18 Emergent Product Dev Uk Ltd Attenuierte salmonella spi2 mutante als antigen-träger
WO2000018964A1 (en) * 1998-09-30 2000-04-06 Boston Medical Center Corporation Identification of virulence determinant activators in prokaryotic pathogens
US6720139B1 (en) 1999-01-27 2004-04-13 Elitra Pharmaceuticals, Inc. Genes identified as required for proliferation in Escherichia coli
US6299850B1 (en) 1999-03-16 2001-10-09 The United States Of America As Represented By The Department Of Energy Carbon activation process for increased surface accessibility in electrochemical capacitors
HK1045856A1 (zh) 1999-04-09 2002-12-13 法玛西雅厄普约翰美国公司 抗细菌疫苗组合物
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GB9910812D0 (en) * 1999-05-10 1999-07-07 Microscience Ltd Vaccine composition
AU5559300A (en) * 1999-07-06 2001-01-22 Institut Pasteur Method of making and identifying attenuated microorganisms, compositions utilizing the sequences responsible for attenuation, and preparations containing attenuated microorganisms
US6589738B1 (en) 1999-11-09 2003-07-08 Elitra Pharmaceuticals, Inc. Genes essential for microbial proliferation and antisense thereto
AU2001230403A1 (en) * 2000-02-03 2001-08-14 Microscience Limited Virulence genes, proteins, and their use
US6783985B1 (en) 2000-02-18 2004-08-31 Elitra Pharmaceuticals Inc. Gene disruption methodologies for drug target discovery
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