ES2126332T3 - Metodo para la identificacion de genes implicados en la adaptacion de un microorganismo a su entorno. - Google Patents

Metodo para la identificacion de genes implicados en la adaptacion de un microorganismo a su entorno.

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Publication number
ES2126332T3
ES2126332T3 ES95939362T ES95939362T ES2126332T3 ES 2126332 T3 ES2126332 T3 ES 2126332T3 ES 95939362 T ES95939362 T ES 95939362T ES 95939362 T ES95939362 T ES 95939362T ES 2126332 T3 ES2126332 T3 ES 2126332T3
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Prior art keywords
stage
environment
microorganisms
nucleic acid
mutant
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Expired - Lifetime
Application number
ES95939362T
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English (en)
Inventor
David William Holden
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Ip2ipo Innovations Ltd
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Imperial College Innovations Ltd
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Filing date
Publication date
Priority claimed from GBGB9424921.6A external-priority patent/GB9424921D0/en
Priority claimed from GBGB9501881.8A external-priority patent/GB9501881D0/en
Priority claimed from GBGB9509239.1A external-priority patent/GB9509239D0/en
Application filed by Imperial College Innovations Ltd filed Critical Imperial College Innovations Ltd
Application granted granted Critical
Publication of ES2126332T3 publication Critical patent/ES2126332T3/es
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
    • C12Q1/04—Determining presence or kind of microorganism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/21—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Pseudomonadaceae (F)
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
    • A61P31/04—Antibacterial agents
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
    • A61P31/10—Antimycotics
    • A—HUMAN NECESSITIES
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    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
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    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/24—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Enterobacteriaceae (F), e.g. Citrobacter, Serratia, Proteus, Providencia, Morganella, Yersinia
    • C07K14/255—Salmonella (G)
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    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/315—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Streptococcus (G), e.g. Enterococci
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    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/315—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Streptococcus (G), e.g. Enterococci
    • C07K14/3156—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Streptococcus (G), e.g. Enterococci from Streptococcus pneumoniae (Pneumococcus)
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    • C07K14/37—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from fungi
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    • C12N1/00—Microorganisms; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
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    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
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    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
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    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00—Medicinal preparations containing antigens or antibodies
    • A61K2039/51—Medicinal preparations containing antigens or antibodies comprising whole cells, viruses or DNA/RNA
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    • Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change

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Abstract

SE DESCRIBE UN METODO PARA IDENTIFICAR MICROORGANISMOS CON ADAPTACION REDUCIDA A UN ENTORNO PARTICULAR. EL METODO CONSTA DE LAS SIGUIENTES ETAPAS: (1) CONSEGUIR UNA PLURALIDAD DE MICROORGANISMOS CADA UNO DE LOS CUALES ES MUTADO INDEPENDIENTEMENTE CON INACTIVACION POR INSERCION DE UN GEN CUYO ACIDO NUCLEICO CONTIENE UNA SECUENCIA MARCADORA UNICA, DE MANERA QUE CADA MUTANTE CONTENGA UNA SECUENCIA MARCADORA DIFERENTE, O CLONES DE DICHO MICROORGANISMO; (2) CONSERVAR UNA MUESTRA DE CADA MUTANTE OBTENIDO EN LA ETAPA (1) Y EL ACIDO NUCLEICO QUE CONTIENE LA SECUENCIA MARCADORA UNICA DE CADA UNO DE LOS MUTANTES; (3) INTRODUCIR UNA PLURALIDAD DE MUTANTES OBTENIDOS EN LA ETAPA (1) EN EL ENTORNO ESPECIFICO Y PERMITIR QUE LOS MICROORGANISMOS QUE SEAN CAPACES DE CRECER LO HAGAN EN ESE ENTORNO; (4) RECUPERAR LOS MICROORGANISMOS DEL ENTORNO O UNA PARTE DE ELLOS Y AISLAR EL ACIDO NUCLEICO DE LOS MICROORGANISMOS RECUPERADOS; (5) COMPARAR LAS SECUENCIA DE LOS MARCADORES DE ACIDO NUCLEICO OBTENIDAS EN LA ETAPA(4) CON LA SECUENCIA DEL MARCADOR UNICO DE CADA MUTANTE CONSERVADO SEGUN SE INDICA EN LA ETAPA (2); Y (6) SELECCIONAR UN MUTANTE QUE NO CONTENGA NINGUNA DE LAS SECUENCIAS MARCADORAS AISLADAS SEGUN SE INDICA EN LA ETAPA (4).
ES95939362T 1994-12-09 1995-12-11 Metodo para la identificacion de genes implicados en la adaptacion de un microorganismo a su entorno. Expired - Lifetime ES2126332T3 (es)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
GBGB9424921.6A GB9424921D0 (en) 1994-12-09 1994-12-09 Identification of genes
GBGB9501881.8A GB9501881D0 (en) 1995-01-31 1995-01-31 Identification of genes
GBGB9509239.1A GB9509239D0 (en) 1995-05-05 1995-05-05 Identification of genes

Publications (1)

Publication Number Publication Date
ES2126332T3 true ES2126332T3 (es) 1999-03-16

Family

ID=27267507

Family Applications (2)

Application Number Title Priority Date Filing Date
ES95939362T Expired - Lifetime ES2126332T3 (es) 1994-12-09 1995-12-11 Metodo para la identificacion de genes implicados en la adaptacion de un microorganismo a su entorno.
ES98201907T Expired - Lifetime ES2178101T3 (es) 1994-12-09 1995-12-11 Genes de virulencia en la region vgc2 de salmonella.

Family Applications After (1)

Application Number Title Priority Date Filing Date
ES98201907T Expired - Lifetime ES2178101T3 (es) 1994-12-09 1995-12-11 Genes de virulencia en la region vgc2 de salmonella.

Country Status (20)

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US (7) US5876931A (es)
EP (3) EP1285960A3 (es)
JP (3) JP3522285B2 (es)
KR (4) KR100551104B1 (es)
CN (3) CN1510137A (es)
AT (2) ATE171477T1 (es)
AU (1) AU711524C (es)
CA (2) CA2206515C (es)
CZ (1) CZ296981B6 (es)
DE (3) DE69526377T2 (es)
DK (2) DK0889120T3 (es)
ES (2) ES2126332T3 (es)
FI (1) FI121601B (es)
HU (1) HUT76975A (es)
NO (2) NO320492B1 (es)
NZ (3) NZ511170A (es)
PT (1) PT889120E (es)
RU (1) RU2370541C2 (es)
SG (4) SG119177A1 (es)
WO (1) WO1996017951A2 (es)

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