ES2179214T3 - Metodos de captura y procedimientos de ensayos. - Google Patents
Metodos de captura y procedimientos de ensayos.Info
- Publication number
- ES2179214T3 ES2179214T3 ES96937431T ES96937431T ES2179214T3 ES 2179214 T3 ES2179214 T3 ES 2179214T3 ES 96937431 T ES96937431 T ES 96937431T ES 96937431 T ES96937431 T ES 96937431T ES 2179214 T3 ES2179214 T3 ES 2179214T3
- Authority
- ES
- Spain
- Prior art keywords
- label
- species
- particles
- captured
- support
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
- 238000000034 method Methods 0.000 title abstract 2
- 238000010998 test method Methods 0.000 title 1
- 239000002245 particle Substances 0.000 abstract 8
- 150000007523 nucleic acids Chemical class 0.000 abstract 2
- 102000039446 nucleic acids Human genes 0.000 abstract 2
- 108020004707 nucleic acids Proteins 0.000 abstract 2
- 108090001008 Avidin Proteins 0.000 abstract 1
- 102000004190 Enzymes Human genes 0.000 abstract 1
- 108090000790 Enzymes Proteins 0.000 abstract 1
- 239000011230 binding agent Substances 0.000 abstract 1
- 239000000975 dye Substances 0.000 abstract 1
- 239000007850 fluorescent dye Substances 0.000 abstract 1
- 229910001385 heavy metal Inorganic materials 0.000 abstract 1
- 238000009396 hybridization Methods 0.000 abstract 1
- 230000001900 immune effect Effects 0.000 abstract 1
- 238000002372 labelling Methods 0.000 abstract 1
- 239000007787 solid Substances 0.000 abstract 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
- G01N33/54313—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals the carrier being characterised by its particulate form
- G01N33/54326—Magnetic particles
- G01N33/54333—Modification of conditions of immunological binding reaction, e.g. use of more than one type of particle, use of chemical agents to improve binding, choice of incubation time or application of magnetic field during binding reaction
Landscapes
- Health & Medical Sciences (AREA)
- Immunology (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Hematology (AREA)
- Biomedical Technology (AREA)
- Urology & Nephrology (AREA)
- Molecular Biology (AREA)
- Medicinal Chemistry (AREA)
- Pathology (AREA)
- Cell Biology (AREA)
- Biotechnology (AREA)
- Food Science & Technology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Physics & Mathematics (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Microbiology (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Solid-Sorbent Or Filter-Aiding Compositions (AREA)
- Peptides Or Proteins (AREA)
- Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
- Enzymes And Modification Thereof (AREA)
- Diaphragms For Electromechanical Transducers (AREA)
- Transition And Organic Metals Composition Catalysts For Addition Polymerization (AREA)
- Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
Abstract
METODOS DE CAPTURA Y MARCADO DE UNA ESPECIE, QUE CONSISTEN EN LA ATRACCION DE PARTICULAS MAGNETICAMENTE ATRAIBLES A UN SOPORTE SOLIDO POR FUERZAS MAGNETICAS, PARTICULAS QUE TIENEN AFINIDAD POR LA ESPECIE UTILIZADA, LA PUESTA EN CONTACTO DE LAS PARTICULAS DEL SOPORTE CON UNA MUESTRA QUE CONTIENE LA ESPECIE EN CUESTION PARA CAPTURAR LA ESPECIE EN LAS PARTICULAS DEL SOPORTE, Y LA UNION DE LA ESPECIE CAPTURADA EN LAS PARTICULAS DE FORMA DIRECTA O INDIRECTA A UN MARCADOR DETECTABLE ANTES Y/O DURANTE LA CAPTURA DE LA ESPECIE EN LAS PARTICULAS DEL SOPORTE. EL MARCADOR PUEDE UNIRSE A LA ESPECIE CAPTURADA A TRAVES DE UN ELEMENTO DE UNION INMUNOLOGICO QUE SE UNE DE FORMA SELECTIVA A LA ESPECIE Y QUE PUEDE SER UN MARCADOR FLUORESCENTE, UN MARCADOR LUMINISCENTE, UN MARCADOR ENZIMATICO, UN MARCADOR COLORANTE, UN MARCADOR FLUORESCENTE, UN MARCADOR QUELANTE DE METALES, UN RADIOMARCADOR, UN MARCADOR DE SPIN, UN MARCADOR DE METALES PESADOS, UN MARCADOR DE HIBRIDACION DE ACIDOS NUCLEICOS O DE ANALOGOS DE ACIDOS NUCLEICOS, UN MARCADOR DE AVIDINA O DE TIPO AVIDINA UNIDO DE MANERA APROPIADA O INCORPORADO A PARTICULAS QUE TAMBIEN LLEVAN UN AGENTE DE UNION COMO UN ANTICUERPO QUE HACE QUE LAS PARTICULAS SE UNAN A LA ESPECIE CAPTURADA.
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| GBGB9523045.4A GB9523045D0 (en) | 1995-11-10 | 1995-11-10 | Methods of capture and asssy procedures |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| ES2179214T3 true ES2179214T3 (es) | 2003-01-16 |
Family
ID=10783693
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| ES96937431T Expired - Lifetime ES2179214T3 (es) | 1995-11-10 | 1996-11-08 | Metodos de captura y procedimientos de ensayos. |
Country Status (11)
| Country | Link |
|---|---|
| EP (1) | EP0861441B1 (es) |
| JP (1) | JP2000500230A (es) |
| AT (1) | ATE221995T1 (es) |
| AU (1) | AU711485B2 (es) |
| CA (1) | CA2237104C (es) |
| DE (1) | DE69622877T2 (es) |
| ES (1) | ES2179214T3 (es) |
| GB (1) | GB9523045D0 (es) |
| NZ (1) | NZ321646A (es) |
| PT (1) | PT861441E (es) |
| WO (1) | WO1997017611A1 (es) |
Families Citing this family (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6159689A (en) * | 1995-11-10 | 2000-12-12 | Genera Technologies Limited | Methods of capture and assay procedures |
| CA2383791C (en) | 1999-09-03 | 2012-11-06 | Miltenyi Biotec Gmbh | Methods of modification of selected cells in a magnetic cell separation column |
| US20050123954A1 (en) * | 2003-09-12 | 2005-06-09 | Biocontrol Systems, Inc. | Methods, compositions, and kits for the concentration and detection of microorganisms |
| US12061200B2 (en) | 2018-12-28 | 2024-08-13 | Abbott Laboratories | Direct detection of single molecules on microparticles |
Family Cites Families (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| IL85921A0 (en) * | 1987-06-10 | 1988-09-30 | Miles Inc | Method,test system and test kit for magnetic separation of labeled reagent in an immunometric binding assay |
| US5108933A (en) * | 1988-09-16 | 1992-04-28 | Immunicon Corporation | Manipulation of colloids for facilitating magnetic separations |
| NZ285139A (en) * | 1994-05-11 | 1998-10-28 | Genera Technologies Ltd | Use of electromagnetism and antibodies to capture microorganisms |
| FI944939A0 (fi) * | 1994-10-20 | 1994-10-20 | Labsystems Oy | Foerfarande foer separering av partiklar |
-
1995
- 1995-11-10 GB GBGB9523045.4A patent/GB9523045D0/en active Pending
-
1996
- 1996-11-08 DE DE69622877T patent/DE69622877T2/de not_active Expired - Lifetime
- 1996-11-08 NZ NZ321646A patent/NZ321646A/en not_active IP Right Cessation
- 1996-11-08 ES ES96937431T patent/ES2179214T3/es not_active Expired - Lifetime
- 1996-11-08 AT AT96937431T patent/ATE221995T1/de not_active IP Right Cessation
- 1996-11-08 AU AU75025/96A patent/AU711485B2/en not_active Expired
- 1996-11-08 JP JP9517996A patent/JP2000500230A/ja active Pending
- 1996-11-08 EP EP96937431A patent/EP0861441B1/en not_active Expired - Lifetime
- 1996-11-08 WO PCT/GB1996/002744 patent/WO1997017611A1/en not_active Ceased
- 1996-11-08 CA CA002237104A patent/CA2237104C/en not_active Expired - Lifetime
- 1996-11-08 PT PT96937431T patent/PT861441E/pt unknown
Also Published As
| Publication number | Publication date |
|---|---|
| CA2237104C (en) | 2004-11-02 |
| AU7502596A (en) | 1997-05-29 |
| JP2000500230A (ja) | 2000-01-11 |
| CA2237104A1 (en) | 1997-05-15 |
| DE69622877D1 (de) | 2002-09-12 |
| PT861441E (pt) | 2002-12-31 |
| EP0861441B1 (en) | 2002-08-07 |
| DE69622877T2 (de) | 2003-04-10 |
| ATE221995T1 (de) | 2002-08-15 |
| AU711485B2 (en) | 1999-10-14 |
| EP0861441A1 (en) | 1998-09-02 |
| GB9523045D0 (en) | 1996-01-10 |
| NZ321646A (en) | 1999-10-28 |
| WO1997017611A1 (en) | 1997-05-15 |
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