ES2180641T3 - Inhibicion citoplasmica de la expresion genetica. - Google Patents

Inhibicion citoplasmica de la expresion genetica.

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Publication number
ES2180641T3
ES2180641T3 ES95921458T ES95921458T ES2180641T3 ES 2180641 T3 ES2180641 T3 ES 2180641T3 ES 95921458 T ES95921458 T ES 95921458T ES 95921458 T ES95921458 T ES 95921458T ES 2180641 T3 ES2180641 T3 ES 2180641T3
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Spain
Prior art keywords
genetic expression
invention concerns
plant cell
plant
cytoplasmic inhibition
Prior art date
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Expired - Lifetime
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ES95921458T
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English (en)
Inventor
Monto H Kumagai
Guy R Della-Cioppa
Jonathan Donson
Damon A Harvey
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Large Scale Biology Corp
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Large Scale Biology Corp
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    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
    • C12N15/1137—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing against enzymes
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Abstract

UN ASPECTO DE LA INVENCION ES PRESENTAR NUEVAS ESTRUCTURAS GENETICAS PARA LA EXPRESION DE UN ARN INHIBIDOR EN EL CITOPLASMA DE CELULAS EUCARIOTICAS. EL ARN INHIBIDOR PUEDE SER UN ARN ANTISENTIDO O UN ARN COSUPRESOR. LAS ESTRUCTURAS GENETICAS DE LA INVENCION PUEDEN REPRODUCIRSE EN EL CITOPLASMA DE UNA CELULA EUCARIOTICA E INCLUIR UNA REGION PROMOTORA EN COMBINACION FUNCIONAL CON UN POLINUCLEOTIDO DE CODIFICACION. LAS ESTRUCTURAS GENETICAS SE PUEDEN DISEÑAR DE MODO QUE SE REPRODUZCAN EN EL CITOPLASMA DE CELULAS DE PLANTAS, CELULAS DE LA LEVADURA Y CELULAS DE MAMIFEROS. CUANDO LA CELULA EUCARIOTICA DE INTERES ES UNA CELULA DE UNA PLANTA, LA ESTRUCTURA GENETICA SE DERIVA PREFERENTEMENTE DE UN VIRUS DE ARN DE LA PLANTA. LAS ESTRUCTURAS GENETICAS DERIVADAS DEL VIRUS DE ARN DE LA PLANTA PUEDEN UTILIZAR UN PROMOTOR SUBGENOMICO DE UN VIRUS DE LA PLANTA, INCLUIDOS PROMOTORES SUBGENOMICOS DE TOBAMOVIRUS EN COMBINACION FUNCIONAL CON LA REGION DE CODIFICACION DEL ARN. OTRO ASPECTO DE LA INVENCION ES PRESENTAR CELULAS QUE CONTIENEN LAS ESTRUCTURAS GENETICAS DE LA INVENCION Y UN ORGANISMO QUE CONTIENE UNA PLURALIDAD DE TALES CELULAS. OTRO ASPECTO DE LA INVENCION ES PRESENTAR METODO PARA REDUCIR LA EXPRESION DE UN GEN DE INTERES EN CELULAS EUCARIOTICAS, A SABER, METODOS PARA LA PRODUCCION DE CELULAS EUCARIOTICAS QUE PRESENTAN NIVELES REDUCIDOS DE EXPRESION DE UN GEN DE INTERES. LOS METODOS DE LA INVENCION INCLUYEN UN PASO QUE CONSISTE EN TRANSFECTAR UNA CELULA CON UNA ESTRUCTURA GENETICA DE LA INVENCION EN DONDE LA REGION QUE CODIFICA EL ARN ES ESPECIFICA AL GEN DE INTERES. OTRO ASPECTO DE LA INVENCION ES PRESENTAR CELULAS DE PLANTAS QUE PRODUCEN NIVELES ELEVADOS DEL FITOENO CAROTENOIDE. LOS NIVELES ELEVADOS DE FITOENO SE CONSIGUEN INHIBIENDO LA EXPRESION EN LA DESATURASA DE FITOENO DE LA ENZIMA MEDIANTE LA UTILIZACION DE LOS VECTORES DE LA INVENCION.
ES95921458T 1994-06-16 1995-05-26 Inhibicion citoplasmica de la expresion genetica. Expired - Lifetime ES2180641T3 (es)

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US08/260,546 US5922602A (en) 1988-02-26 1994-06-16 Cytoplasmic inhibition of gene expression

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ES95921458T Expired - Lifetime ES2180641T3 (es) 1994-06-16 1995-05-26 Inhibicion citoplasmica de la expresion genetica.
ES00127988T Expired - Lifetime ES2246210T3 (es) 1994-06-16 1995-05-26 Inhibicion citoplasmatica de la expresion genetica.

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