PT663921E - Metodo para aumentar a expressao e reduzir a variabilidade de expressao de genes estranhos em celulas vegetais - Google Patents

Metodo para aumentar a expressao e reduzir a variabilidade de expressao de genes estranhos em celulas vegetais

Info

Publication number
PT663921E
PT663921E PT93922757T PT93922757T PT663921E PT 663921 E PT663921 E PT 663921E PT 93922757 T PT93922757 T PT 93922757T PT 93922757 T PT93922757 T PT 93922757T PT 663921 E PT663921 E PT 663921E
Authority
PT
Portugal
Prior art keywords
expression
transcription initiation
initiation region
variability
reducing
Prior art date
Application number
PT93922757T
Other languages
English (en)
Inventor
William F Thomson
Steven L Spiker
George C Allen
Gerald E Hall
Lisa C Childs
Original Assignee
Univ North Carolina State
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Univ North Carolina State filed Critical Univ North Carolina State
Publication of PT663921E publication Critical patent/PT663921E/pt

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/37—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from fungi
    • C07K14/39—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from fungi from yeasts
    • C07K14/395—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from fungi from yeasts from Saccharomyces
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C12N15/822—Reducing position variability, e.g. by the use of scaffold attachment region/matrix attachment region (SAR/MAR); Use of SAR/MAR to regulate gene expression

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Cell Biology (AREA)
  • Mycology (AREA)
  • Physics & Mathematics (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Preparation Of Fruits And Vegetables (AREA)
PT93922757T 1992-10-05 1993-09-27 Metodo para aumentar a expressao e reduzir a variabilidade de expressao de genes estranhos em celulas vegetais PT663921E (pt)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US95642092A 1992-10-05 1992-10-05

Publications (1)

Publication Number Publication Date
PT663921E true PT663921E (pt) 2002-10-31

Family

ID=25498228

Family Applications (1)

Application Number Title Priority Date Filing Date
PT93922757T PT663921E (pt) 1992-10-05 1993-09-27 Metodo para aumentar a expressao e reduzir a variabilidade de expressao de genes estranhos em celulas vegetais

Country Status (10)

Country Link
US (1) US5773689A (pt)
EP (1) EP0663921B1 (pt)
AT (1) ATE217319T1 (pt)
AU (1) AU673859B2 (pt)
CA (1) CA2147006C (pt)
DE (1) DE69331908T2 (pt)
DK (1) DK0663921T3 (pt)
ES (1) ES2176208T3 (pt)
PT (1) PT663921E (pt)
WO (1) WO1994007902A1 (pt)

Families Citing this family (30)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6239328B1 (en) 1992-10-05 2001-05-29 North Carolina State University Method for reducing expression variability of transgenes in plant cells
WO1994024293A1 (en) * 1993-04-19 1994-10-27 Sandoz Ltd. Genetic stabilizing elements
US6040185A (en) * 1993-04-19 2000-03-21 Novartis Ag Genetic stabilizing elements
US5773695A (en) * 1996-01-26 1998-06-30 North Carolina State University Plant nuclear scaffold attachment region and method for increasing gene expression in transgenic cells
US6037525A (en) * 1996-08-01 2000-03-14 North Carolina State University Method for reducing expression variability of transgenes in plant cells
KR20000049209A (ko) * 1996-10-17 2000-07-25 메리 이. 보울러 스캐폴드 부착 부위를 이용한 단자엽 세포 군집에서 증진된 트랜스진의 발현
CA2283463A1 (en) * 1997-03-28 1998-10-08 Mycogen Plant Science, Inc. Improved sar plant transformation process
EP1003849A4 (en) * 1997-06-03 2004-06-16 Univ North Carolina State METHOD FOR REDUCING THE EXPRESSION VARIANCE OF TRANSGENES IN PLANT CELLS
US6245974B1 (en) * 1997-08-06 2001-06-12 North Carolina State University Matrix attachment regions
US6518485B1 (en) * 1998-06-04 2003-02-11 Westvaco Corporation Particle-mediated conifer transformation
WO2000006757A1 (en) * 1998-07-31 2000-02-10 Mycogen Plant Science, Inc. Improved plant transformation process by scaffold attachment regions (sar)
WO2000018938A1 (en) * 1998-09-29 2000-04-06 Pioneer Hi-Bred International, Inc. Mar/sar elements flanking rsyn7-driven construct
US6482646B1 (en) 1998-11-06 2002-11-19 E. I. Du Pont De Nemours And Company Plant proteins that interact with nuclear matrix proteins and function as transcriptional activators
WO2000032800A1 (en) * 1998-12-01 2000-06-08 Dow Agrosciences Llc Artificial matrix attachment region for increasing expression of genes introduced in plant cells
US6395962B1 (en) 1999-06-22 2002-05-28 University Of South Carolina Enhancing expression of a silenced target sequence in plants using plant viral enhancers and amplicons
US7126041B1 (en) 1999-12-10 2006-10-24 North Carolina State Unversity High efficiency gene targeting in plants
KR100408844B1 (ko) 2000-07-29 2003-12-06 한국산업기술평가원 동물세포 발현벡터
CA2426856A1 (en) * 2000-10-25 2002-08-08 Washington State University Research Foundation Thuja plicata dirigent protein promoters
DK1395669T3 (da) 2001-01-26 2009-11-16 Selexis Sa Matriks bindingsregioner og fremgangsmåder til anvendelse af disse
DE10114209A1 (de) * 2001-03-23 2002-12-05 Icon Genetics Ag Ortsgerichtete Transformation durch Verwendung von Amplifikationsvektoren
EP1279737A1 (en) 2001-07-27 2003-01-29 Coöperatieve Verkoop- en Productievereniging, van Aardappelmeel en Derivaten AVEBE B.A. Transformation method for obtaining marker-free plants
US20040016014A1 (en) * 2002-07-17 2004-01-22 Nguyen Thanh-Tuyen T. High efficiency germline transformation system
US20040016015A1 (en) * 2002-07-17 2004-01-22 Nguyen Thanh-Tuyen T. High efficiency germline transformation system
AU2004266694A1 (en) * 2003-08-19 2005-03-03 22Nd Century Limited, Llc Reduced-exposure tobacco products
KR101269656B1 (ko) 2003-10-24 2013-05-30 셀렉시스 에스. 에이. Ｍａｒ 서열의 복합 트랜스펙션 방법에 의한 포유동물 세포에서의 고효율 유전자 전달 및 발현
AU2006312126A1 (en) 2005-11-04 2007-05-18 Dow Agrosciences Llc Preparation of vaccine master cell lines using recombinant plant suspension cultures
AU2007353773A1 (en) 2006-12-22 2008-11-27 Dow Agrosciences Llc Plant-made West Nile virus (WNV) vaccines, vectors and plant codon optimized sequences
PL2655629T3 (pl) * 2010-12-24 2024-10-28 BASF Agricultural Solutions Seed US LLC Roślina brassica zawierająca zmutowany allel alcatraz
EP2761003A4 (en) * 2011-09-15 2015-11-11 Basf Plant Science Co Gmbh REGULATORY NUCLEIC ACID MOLECULES FOR RELIABLE GENE EXPRESSION IN PLANTS
EP3782465B1 (en) 2019-08-23 2023-08-30 Universität Potsdam Transgenic zebrafish model for stroke

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5122466A (en) * 1989-06-13 1992-06-16 North Carolina State University Ballistic transformation of conifers
US5550318A (en) * 1990-04-17 1996-08-27 Dekalb Genetics Corporation Methods and compositions for the production of stably transformed, fertile monocot plants and cells thereof
AU7583691A (en) * 1990-03-05 1991-10-10 Upjohn Company, The Protein expression via seed specific regulatory sequences
US5187267A (en) * 1990-06-19 1993-02-16 Calgene, Inc. Plant proteins, promoters, coding sequences and use
MX9200621A (es) * 1991-02-14 1993-02-01 Du Pont Gen de una proteina con alto contenido de azufre de una semilla y metodo para aumentar el contenido de azufre en aminoacidos de las plantas.
DK1394257T3 (da) * 1992-03-19 2007-12-10 Du Pont Nukleinsyrefragmenter og fremgangsmåder til forögelse af lysin- og threonin-indholdet i fröene fra planter
WO1994024293A1 (en) * 1993-04-19 1994-10-27 Sandoz Ltd. Genetic stabilizing elements

Also Published As

Publication number Publication date
DE69331908D1 (de) 2002-06-13
DE69331908T2 (de) 2002-12-12
EP0663921A1 (en) 1995-07-26
AU5165593A (en) 1994-04-26
WO1994007902A1 (en) 1994-04-14
ATE217319T1 (de) 2002-05-15
CA2147006C (en) 2004-11-30
AU673859B2 (en) 1996-11-28
EP0663921A4 (en) 1996-07-17
US5773689A (en) 1998-06-30
ES2176208T3 (es) 2002-12-01
DK0663921T3 (da) 2002-09-02
EP0663921B1 (en) 2002-05-08
CA2147006A1 (en) 1994-04-14

Similar Documents

Publication Publication Date Title
PT663921E (pt) Metodo para aumentar a expressao e reduzir a variabilidade de expressao de genes estranhos em celulas vegetais
NZ333998A (en) Method for reducing expression variability of transgenes in plant cells using DNA constructs comprising insulator elements
NZ313474A (en) Root cortex specific gene promoter
BR8401781A (pt) Processo para modificar geneticamente uma celula vegetal e vegetal,tecido vegetal ou celula vegetal produzido por tal processo
CA2193094A1 (en) The cytoplasmic inhibition of gene expression
WO1997004114A3 (fr) Sequence adn isolee pouvant servir de zone de regulation dans un gene chimere utilisable pour la transformation des plantes
ATE104699T1 (de) Hervorrufung physischer veraenderungen in pflanzen durch verwendung von negativstrangigen rnas.
DE3471688D1 (en) Dna vectors and their use in recombinant dna technology
EP0260148A3 (en) Improved recombinant expression method, vector and transformed cells
EP0513849A3 (en) Zea mays plants and transgenic zea mays plants regenerated from protoplasts or protoplast-derived cells
ZA939767B (en) Nematode-resistant transgenic plants
ATE64416T1 (de) Die verwendung des gal 1 hefe-promotors.
HUT52158A (en) Process for producing new chimeric gene
EP0249676A3 (en) Method for the expression of genes in plants
EP0320500A3 (en) Non-oncogenic ti plasmid vector system and recombinant dna molecules for the introduction of expressible genes into plant cell genomes
ATE92530T1 (de) Transformation und expression eines fremden gens in -i(brassica) spezies.
FR2772787B1 (fr) Promoteur h3c4 de mais associe au premier intron de l'actine de riz, gene chimere le comprenant et plante transformee
EP0854916A4 (en) NEW ACC SYNTHASE GENES
FI924909A0 (fi) Biosyntetiskt foerfarande foer framstaellning av kemiska foereningar
EP0336383A3 (en) Novel recombinant human lymphotoxin
CA2244204A1 (en) A plant nuclear scaffold attachment region which increases gene expression
MX9701598A (es) Un promotor de plantas util para dirigir la expresion de proteinas extrañas a la epidermis de la planta.
KR960023060A (ko) 에리스로포이에틴의 제조방법
CA2309028A1 (en) The cytoplasmic inhibition of gene expression
ATE122097T1 (de) Expressionsvektor und verfahren zur regulierbaren herstellung von proteinen in eukaryonten.