ES2182121T3 - Metodos para marcar nucleotidos, nucleotidos marcados e intermediarios utiles. - Google Patents

Metodos para marcar nucleotidos, nucleotidos marcados e intermediarios utiles.

Info

Publication number
ES2182121T3
ES2182121T3 ES97943223T ES97943223T ES2182121T3 ES 2182121 T3 ES2182121 T3 ES 2182121T3 ES 97943223 T ES97943223 T ES 97943223T ES 97943223 T ES97943223 T ES 97943223T ES 2182121 T3 ES2182121 T3 ES 2182121T3
Authority
ES
Spain
Prior art keywords
nucleotides
chain
spacer
moiety
linker
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
ES97943223T
Other languages
English (en)
Inventor
Hendrik Jan Houthoff
Jan Reedijk
Tinka Jelsma
Robert Jochem Heetebrij
Herman Hendricus Volkers
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Kreatech Biotechnology BV
Original Assignee
Kreatech Biotechnology BV
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Kreatech Biotechnology BV filed Critical Kreatech Biotechnology BV
Application granted granted Critical
Publication of ES2182121T3 publication Critical patent/ES2182121T3/es
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H23/00—Compounds containing boron, silicon or a metal, e.g. chelates or vitamin B12
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S436/00—Chemistry: analytical and immunological testing
    • Y10S436/80—Fluorescent dyes, e.g. rhodamine
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
    • Y10T436/00—Chemistry: analytical and immunological testing
    • Y10T436/14—Heterocyclic carbon compound [i.e., O, S, N, Se, Te, as only ring hetero atom]
    • Y10T436/142222—Hetero-O [e.g., ascorbic acid, etc.]
    • Y10T436/143333—Saccharide [e.g., DNA, etc.]

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Genetics & Genomics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Engineering & Computer Science (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Cold Cathode And The Manufacture (AREA)
  • Inorganic Insulating Materials (AREA)
  • Medicinal Preparation (AREA)

Abstract

EN LA PRESENTE INVENCION SE PRESENTAN PROCEDIMIENTOS MEJORADOS PARA ETIQUETAR NUCLEOTIDOS. EL PROCEDIMIENTO DE LA INVENCION DE ETIQUETADO DE NUCLEOTIDOS CONSISTE EN LOS PASOS SIGUIENTES: SE HACE REACCIONAR UNA FRACCION REACTIVA DE UN LINKER, SIENDO EL LINKER UN COMPUESTO DE PLATINO QUE TIENE UN PUENTE DE ESTABILIZACION Y DOS FRACCIONES REACTIVAS, CON UNA FRACCION DONADORA DE ELECTRONES DE UN SEPARADOR, COMPRENDIENDO EL SEPARADOR UNA CADENA QUE TIENE AL MENOS CUATRO ATOMOS Y AL MENOS UN HETEROATOMO EN LA CADENA, Y COMPRENDIENDO ADEMAS EL SEPARADOR DICHA FRACCION DONADORA DE ELECTRONES EN UN EXTREMO DE LA CADENA Y UNA FRACCION REACTIVA EN EL OTRO EXTREMO DE LA CADENA; SE HACE REACCIONAR LA FRACCION REACTIVA DE DICHO SEPARADOR CON UNA ETIQUETA; Y SE HACE REACCIONAR LA OTRA UNIDAD REACTIVA DEL LINKER ANTEDICHO CON UN NUCLEOTIDO. UNA GRAN VENTAJA DE LA INVENCION ES QUE SEGUN EL PROCEDIMIENTO DE LA INVENCION SE PUEDE ETIQUETAR TODO TIPO DE NUCLEOTIDOS CUANDO HASTA LA FECHA LA APLICACION DE UNA ETIQUETA QUEDABA SOBRE TODO RESTRINGIDA A UN NUCLEOTIDO O A CIERTO TIPO DE NUCLEOTIDOS.
ES97943223T 1996-10-08 1997-10-08 Metodos para marcar nucleotidos, nucleotidos marcados e intermediarios utiles. Expired - Lifetime ES2182121T3 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
EP96202792 1996-10-08

Publications (1)

Publication Number Publication Date
ES2182121T3 true ES2182121T3 (es) 2003-03-01

Family

ID=8224469

Family Applications (1)

Application Number Title Priority Date Filing Date
ES97943223T Expired - Lifetime ES2182121T3 (es) 1996-10-08 1997-10-08 Metodos para marcar nucleotidos, nucleotidos marcados e intermediarios utiles.

Country Status (12)

Country Link
US (4) US6338943B1 (es)
EP (1) EP0937090B1 (es)
JP (2) JP4981197B2 (es)
AT (1) ATE221894T1 (es)
AU (1) AU726692B2 (es)
CA (1) CA2267133C (es)
DE (1) DE69714600T2 (es)
DK (1) DK0937090T3 (es)
ES (1) ES2182121T3 (es)
NZ (1) NZ334803A (es)
PT (1) PT937090E (es)
WO (1) WO1998015564A1 (es)

Families Citing this family (19)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ATE221894T1 (de) * 1996-10-08 2002-08-15 Kreatech Biotech Bv Verfahren zur markierung von nukleotiden, markierte nukleotide und nuetzliche zwischenprodukte
US6458373B1 (en) 1997-01-07 2002-10-01 Sonus Pharmaceuticals, Inc. Emulsion vehicle for poorly soluble drugs
EP1006199A1 (en) * 1998-12-03 2000-06-07 Kreatech Biotechnology B.V. Applications with and methods for producing selected interstrand crosslinks in nucleic acid
EP1218547A4 (en) * 1999-10-15 2005-04-20 Ventana Med Syst Inc METHOD OF IN-SITU DETECTION OF INDIVIDUAL GENOCOPIES
US6376669B1 (en) * 1999-11-05 2002-04-23 3M Innovative Properties Company Dye labeled imidazoquinoline compounds
GB0016836D0 (en) 2000-07-07 2000-08-30 Lee Helen Improved dipstick assays (1)
EP1373572B1 (en) 2001-03-02 2006-07-26 Stratagene California Compositions and methods using platinum compounds for nucleic acid labeling
ATE376675T1 (de) * 2001-05-28 2007-11-15 Kreatech Biotech Bv Ein verfahren zur differentiellen markierung mittels platin-komplexen
US7166478B2 (en) * 2002-03-12 2007-01-23 Enzo Life Sciences, Inc., C/O Enzo Biochem, Inc. Labeling reagents and labeled targets, target labeling processes and other processes for using same in nucleic acid determinations and analyses
JP2005538735A (ja) * 2002-09-17 2005-12-22 パーキネルマー ラス インコーポレイテッド 核酸反応のリアルタイム検出法
US20050028450A1 (en) * 2003-08-07 2005-02-10 Wen-Qing Xu CMP slurry
US20060281100A1 (en) * 2005-06-14 2006-12-14 Shen Gene G Thiotriphosphate nucleotide dye terminators
EP1745802A1 (en) 2005-07-20 2007-01-24 Kreatech Biotechnology B.V. Method of conjugating therapeutic compounds to cell targeting moieties via metal complexes
JP5256026B2 (ja) * 2006-04-27 2013-08-07 株式会社ワンセル ヌクレオチド−遷移金属錯体触媒
EP2944647A1 (en) 2007-07-26 2015-11-18 Cellay, Inc. Highly visible chromosome-specific probes and related methods
KR101072900B1 (ko) * 2007-12-04 2011-10-17 주식회사 파나진 고정화된 펩티드핵산 프로브를 사용한 표적핵산의 선택적 표지 및 검출 방법
JP6120416B2 (ja) 2012-01-06 2017-04-26 リンクシス ビー．ヴイ． 細胞標的コンジュゲートを調製するための方法、及び得られる錯体
CN109030698B (zh) * 2018-08-06 2020-09-04 泰山医学院 一种液相色谱法检测固体福美钠纯度的方法
CN108760961B (zh) * 2018-08-06 2020-09-22 青岛中科荣达新材料有限公司 一种液相色谱法检测固体福美钾纯度的方法

Family Cites Families (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4207416A (en) * 1974-09-05 1980-06-10 Engelhard Minerals & Chemicals Corporation Ethylenediamineplatinum(II) 2,4-dioxopyrimidine complexes
JPS6087295A (ja) * 1983-10-19 1985-05-16 Nippon Kayaku Co Ltd 新規白金錯体
DE3568063D1 (en) * 1985-01-10 1989-03-09 Molecular Diagnostics Inc Photochemical method of labelling nucleic acids for detection in hybridization assays
JPS63203692A (ja) * 1987-02-19 1988-08-23 Nippon Kayaku Co Ltd 新規白金錯体
WO1989004317A1 (fr) * 1987-11-06 1989-05-18 Sagami Chemical Research Center Nouveaux complexes de platine (ii)
US5552541A (en) * 1990-06-20 1996-09-03 Beckman Instruments, Inc. Haptenic probes for detecting capture polynucleotides
NL9001639A (nl) * 1990-07-19 1992-02-17 Amc Amsterdam Pt-houdende verbinding, werkwijze voor de bereiding ervan, alsmede toepassing van dergelijke verbindingen.
US5714327A (en) * 1990-07-19 1998-02-03 Kreatech Diagnostics Platinum-containing compounds, methods for their preparation and applications thereof
EP1138334A3 (en) * 1992-06-09 2002-04-03 Neorx Corporation Pretargeting methods and compounds
JPH0698797A (ja) * 1992-09-22 1994-04-12 Toyobo Co Ltd 核酸検出法
DE4344742A1 (de) * 1993-06-09 1994-12-15 Boehringer Mannheim Gmbh Verfahren zur Immobilisierung von Nukleinsäuren
DE4326466A1 (de) * 1993-08-06 1995-02-09 Boehringer Mannheim Gmbh Infrarot-Farbstoff-markierte Nucleotide und ihre Verwendung in der Nucleinsäure-Detektion
WO1996035696A1 (en) * 1995-05-09 1996-11-14 Kreatech Biotechnology B.V. Methods for the production of platinum-based linkers between labels and bio-organic molecules, for labelling bio-organic molecules, for detecting biological substances of interest and diagnostic test kits
ATE221894T1 (de) * 1996-10-08 2002-08-15 Kreatech Biotech Bv Verfahren zur markierung von nukleotiden, markierte nukleotide und nuetzliche zwischenprodukte

Also Published As

Publication number Publication date
CA2267133A1 (en) 1998-04-16
DK0937090T3 (da) 2002-12-02
US20020160396A1 (en) 2002-10-31
JP4981197B2 (ja) 2012-07-18
CA2267133C (en) 2006-07-18
JP2010166914A (ja) 2010-08-05
AU726692B2 (en) 2000-11-16
US6338943B1 (en) 2002-01-15
JP2001503742A (ja) 2001-03-21
PT937090E (pt) 2002-12-31
DE69714600D1 (de) 2002-09-12
EP0937090B1 (en) 2002-08-07
US7217813B2 (en) 2007-05-15
US6797818B2 (en) 2004-09-28
US20050118621A1 (en) 2005-06-02
AU4474697A (en) 1998-05-05
NZ334803A (en) 2000-05-26
WO1998015564A1 (en) 1998-04-16
ATE221894T1 (de) 2002-08-15
EP0937090A1 (en) 1999-08-25
DE69714600T2 (de) 2003-03-27
USRE40557E1 (en) 2008-10-28

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