ES2183166T3 - Ensayo de cribado por triple hibrido. - Google Patents

Ensayo de cribado por triple hibrido.

Info

Publication number
ES2183166T3
ES2183166T3 ES97921370T ES97921370T ES2183166T3 ES 2183166 T3 ES2183166 T3 ES 2183166T3 ES 97921370 T ES97921370 T ES 97921370T ES 97921370 T ES97921370 T ES 97921370T ES 2183166 T3 ES2183166 T3 ES 2183166T3
Authority
ES
Spain
Prior art keywords
molecula
binding
hybrid
expression
vector
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
ES97921370T
Other languages
English (en)
Inventor
Jun Liu
Edward J Licitra
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Massachusetts Institute of Technology
Original Assignee
Massachusetts Institute of Technology
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Massachusetts Institute of Technology filed Critical Massachusetts Institute of Technology
Application granted granted Critical
Publication of ES2183166T3 publication Critical patent/ES2183166T3/es
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/536—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase
    • G01N33/542—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase with steric inhibition or signal modification, e.g. fluorescent quenching
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1055—Protein x Protein interaction, e.g. two hybrid selection
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/62—DNA sequences coding for fusion proteins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6897—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids involving reporter genes operably linked to promoters
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/61—Fusion polypeptide containing an enzyme fusion for detection (lacZ, luciferase)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/70—Fusion polypeptide containing domain for protein-protein interaction
    • C07K2319/71—Fusion polypeptide containing domain for protein-protein interaction containing domain for transcriptional activaation, e.g. VP16
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/70—Fusion polypeptide containing domain for protein-protein interaction
    • C07K2319/71—Fusion polypeptide containing domain for protein-protein interaction containing domain for transcriptional activaation, e.g. VP16
    • C07K2319/715—Fusion polypeptide containing domain for protein-protein interaction containing domain for transcriptional activaation, e.g. VP16 containing a domain for ligand dependent transcriptional activation, e.g. containing a steroid receptor domain
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/80—Fusion polypeptide containing a DNA binding domain, e.g. Lacl or Tet-repressor
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/80—Fusion polypeptide containing a DNA binding domain, e.g. Lacl or Tet-repressor
    • C07K2319/81—Fusion polypeptide containing a DNA binding domain, e.g. Lacl or Tet-repressor containing a Zn-finger domain for DNA binding

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Biomedical Technology (AREA)
  • Organic Chemistry (AREA)
  • Molecular Biology (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • Wood Science & Technology (AREA)
  • Immunology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Plant Pathology (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Cell Biology (AREA)
  • Medicinal Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Food Science & Technology (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)

Abstract

SE PROPORCIONAN UN PROCEDIMIENTO Y KIT PARA IDENTIFICAR Y CARACTERIZAR UN COMPONENTE CELULAR O MOLECULA PEQUEÑA. EL PROCEDIMIENTO Y KIT INCLUYEN UNA MOLECULA HIBRIDA DE LIGANDO (7) FORMADA POR DOS LIGANDOS, DE MANERA QUE UNO DE ELLOS SEA LA MOLECULA PEQUEÑA (7B) CUYA ESPECIFICIDAD SE DESEA DETERMINAR, MIENTRAS QUE EL OTRO LIGANDO TIENE ESPECIFICIDAD PARA UN BLANCO PREDETERMINADO (7A). EL LIGANDO HIBRIDO SE INTRODUCE EN UN ENTORNO QUE CONTIENE: UN PRIMER VECTOR DE EXPRESION QUE CODIFICA UN BLANCO PARA UNO DE LOS LIGANDOS VINCULADOS A UNA SECUENCIA DE CODIFICACION PARA UN PRIMER MODULO TRANSCRIPCIONAL (8); Y UN SEGUNDO VECTOR DE EXPRESION QUE INCLUYE UN BLANCO PARA EL OTRO LIGANDO VINCULADO A UNA SECUENCIA DE CODIFICACION DE UN SEGUNDO MODULO TRANSCRIPCIONAL (9). EL ENTORNO CONTIENE ADEMAS UN TERCER VECTOR (6) QUE INCLUYE UN GEN INFORMADOR, EN EL QUE LA EXPRESION DEL GEN REPORTER (5) DEPENDE DE LA PROXIMIDAD DE LOS MODULOS TRANSCRIPCIONALES PRIMERO Y SEGUNDO, QUE QUEDA FACILITADA POR LA FIJACION DE AMBOS LIGANDOS DE LA MOLECULA HIBRIDA DE LIGANDOS A SUS BLANCOS.
ES97921370T 1996-04-26 1997-04-25 Ensayo de cribado por triple hibrido. Expired - Lifetime ES2183166T3 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US1734196P 1996-04-26 1996-04-26

Publications (1)

Publication Number Publication Date
ES2183166T3 true ES2183166T3 (es) 2003-03-16

Family

ID=21782024

Family Applications (1)

Application Number Title Priority Date Filing Date
ES97921370T Expired - Lifetime ES2183166T3 (es) 1996-04-26 1997-04-25 Ensayo de cribado por triple hibrido.

Country Status (8)

Country Link
US (1) US5928868A (es)
EP (1) EP0907750B1 (es)
JP (1) JP2000508923A (es)
AT (1) ATE224454T1 (es)
CA (1) CA2252886C (es)
DE (1) DE69715621T2 (es)
ES (1) ES2183166T3 (es)
WO (1) WO1997041255A1 (es)

Families Citing this family (60)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6972193B1 (en) * 1993-02-12 2005-12-06 Board Of Trustees Of Leland Stanford Junior University Regulated transcription of targeted genes and other biological events
US6891021B2 (en) * 1993-02-12 2005-05-10 Board Of Trustees Of The Leland Stanford Junior University Regulated apoptosis
US6841347B1 (en) * 1998-02-05 2005-01-11 The General Hospital Corporation In vivo construction of DNA libraries
US6187535B1 (en) 1998-02-18 2001-02-13 Institut Pasteur Fast and exhaustive method for selecting a prey polypeptide interacting with a bait polypeptide of interest: application to the construction of maps of interactors polypeptides
EP1088229A4 (en) * 1998-06-25 2002-05-08 Caliper Techn Corp HIGH THROUGHPUT METHODS, SYSTEMS AND APPARATUS FOR CELL SCREENING TEST
CA2338247A1 (en) * 1998-07-28 2000-02-10 Vimal D. Mehta General screening method for ligand-protein interactions
EP1792989A1 (en) 1999-04-12 2007-06-06 Agensys, Inc. 13 Transmembrane protein expressed in prostate cancer
IL145775A0 (en) 1999-04-12 2002-07-25 Agensys Inc 13 transmembrane protein expressed in prostate cancer
DE60045201D1 (de) 1999-08-12 2010-12-16 Agensys Inc C-Typ-Lektin-Transmembran-Antigen, das in menschlichem Prostatakrebs exprimiert wird, und Verwendungen dieses Antigens
DK1220913T3 (da) 1999-10-05 2009-03-16 Agensys Inc G-protein-koblet-receptor opreguleret i prostatacancer og anvendelser deraf
US6893818B1 (en) * 1999-10-28 2005-05-17 Agensys, Inc. Gene upregulated in cancers of the prostate
DE60038127T2 (de) * 2000-01-06 2009-03-05 Caliper Life Sciences, Inc., Mountain View Methoden und syteme zur überwachung intrazellulärer bindereaktionen
EP1254179A4 (en) * 2000-01-24 2005-06-01 Univ Columbia IN VIVO SCREEN USING CHEMICAL INDUCTORS
US6406863B1 (en) * 2000-06-23 2002-06-18 Genetastix Corporation High throughput generation and screening of fully human antibody repertoire in yeast
US6410246B1 (en) 2000-06-23 2002-06-25 Genetastix Corporation Highly diverse library of yeast expression vectors
US6410271B1 (en) 2000-06-23 2002-06-25 Genetastix Corporation Generation of highly diverse library of expression vectors via homologous recombination in yeast
ATE429643T1 (de) 2000-07-12 2009-05-15 Agensys Inc Neues tumor antigen das für diagnose und therapie von blasen-, eierstock-, lungen- und nierenkrebs verwendet werden kann
CA2420543C (en) 2000-08-22 2013-04-02 Agensys, Inc. Nucleic acid and corresponding protein named 158p1d7 useful in the treatment and detection of bladder and other cancers
ES2311540T3 (es) 2000-08-28 2009-02-16 Agensys, Inc. Acido nucleico y proteina correspondiente llamada 85p1b3 util en el tratamiento y deteccion del cancer.
HRP20010018A2 (en) 2001-01-09 2002-12-31 Pliva D D Novel anti-inflammatory compounds
US20020168737A1 (en) * 2001-01-24 2002-11-14 Cornish Virginia W. Binding and catalysis screen for high throughput determination of protein function using chemical inducers of dimerization
US20030165873A1 (en) * 2001-03-02 2003-09-04 Come Jon H. Three hybrid assay system
US6924358B2 (en) 2001-03-05 2005-08-02 Agensys, Inc. 121P1F1: a tissue specific protein highly expressed in various cancers
US7271240B2 (en) 2001-03-14 2007-09-18 Agensys, Inc. 125P5C8: a tissue specific protein highly expressed in various cancers
US20030191073A1 (en) 2001-11-07 2003-10-09 Challita-Eid Pia M. Nucleic acid and corresponding protein entitled 161P2F10B useful in treatment and detection of cancer
EP2280030A3 (en) 2001-04-10 2011-06-15 Agensys, Inc. Nucleic acids and corresponding proteins useful in the detection and treatment of various cancers
CA2444857A1 (en) * 2001-04-20 2002-10-31 President And Fellows Of Harvard College Compositions and methods for the identification of protein interactions in vertebrate cells
AU2002336446B2 (en) 2001-09-06 2008-03-06 Agensys, Inc. Nucleic acid and corresponding protein entitled STEAP-1 useful in treatment and detection of cancer
DE10220593A1 (de) * 2001-11-28 2003-06-12 Graffinity Pharmaceuticals Ag SPR-Sensorflächenträger
AU2002352069A1 (en) * 2001-11-28 2003-06-10 Graffinity Pharmaceuticals Ag Surface plasmon resonance (spr) sensor surface support
WO2003046198A2 (de) * 2001-11-28 2003-06-05 Graffinity Pharmaceuticals Ag Verfahren zur selektion und identifikation von peptid- oder proteinmolekülen mittels phage display
US20030138785A1 (en) * 2001-12-21 2003-07-24 Stephan Kopytek In vivo protein screen based on enzyme-assisted chemically induced dimerization ("CID")
US20040186063A1 (en) * 2002-02-15 2004-09-23 Hans-Jurgen Gutke Conjugates of biologically active compounds, methods for their preparation and use, formulation and pharmaceutical applications thereof
WO2003070174A2 (en) * 2002-02-15 2003-08-28 Sympore Gmbh Conjugates of biologically active compounds, methods for their preparation and use, formulation and pharmaceutical applications thereof
WO2003070254A1 (en) * 2002-02-15 2003-08-28 Sympore Gmbh Antibiotic conjugates
EP1483579A4 (en) * 2002-02-15 2006-07-12 Merckle Gmbh CONJUGATES OF BIOLOGICALLY ACTIVE COMPOUNDS, PROCESS FOR THEIR MANUFACTURE AND USE, FORMULATION AND PHARMACEUTICAL APPLICATIONS THEREOF
US7083918B2 (en) * 2002-04-24 2006-08-01 The Trustees Of Columbia University In The City Of New York Bacterial small-molecule three-hybrid system
EP1551865B1 (en) 2002-07-08 2009-04-22 GlaxoSmithKline istrazivacki centar Zagreb d.o.o. Hybrid molecules of macrolides with steroidal/non-steroidal anti-inflammatory molecules
NZ537716A (en) 2002-07-08 2007-05-31 Glaxosmithkline Zagreb Novel nonsteroidal anti-inflammatory substances, compositions and methods for their use
AU2003255851B2 (en) 2002-07-08 2009-07-23 Glaxosmithkline Istrazivacki Centar Zagreb D.O.O. New compounds, compositions and methods for treatment of inflammatory diseases and conditions
JP2005532071A (ja) * 2002-07-10 2005-10-27 ストラタジーン カリフォルニア 足場としてのヒト化レニラ・レニフォルミス緑色蛍光タンパク質
US20040067532A1 (en) * 2002-08-12 2004-04-08 Genetastix Corporation High throughput generation and affinity maturation of humanized antibody
AU2003243151A1 (en) 2002-08-16 2004-03-03 Agensys, Inc. Nucleic acid and corresponding protein entitled 251p5g2 useful in treatment and detection of cancer
EP2343315A3 (en) 2003-02-10 2011-11-23 Agensys, Inc. Nucleic acid and corresponding protein named 158P1D7 useful in the treatment and detection of bladder and other cancers
KR101291787B1 (ko) 2003-05-30 2013-08-07 어젠시스 인코포레이티드 전립선 줄기 세포 항원 (ｐｓｃａ) 변이체 및 그의서브서열
EP2428522A1 (en) 2004-05-28 2012-03-14 Agensys, Inc. Antibodies that bind to PSCA proteins for diagnosis of cancer
US8034553B2 (en) 2004-06-24 2011-10-11 Kimberly-Clark Worldwide, Inc. Biomarkers for wound healing
WO2006008092A1 (en) * 2004-07-16 2006-01-26 Novartis Ag Use of a steroid for enhancement of skin permeability
WO2006046694A1 (ja) * 2004-10-29 2006-05-04 Eisai R & D Management Co., Ltd. 誘導可能に発現するエルゴステロール合成酵素を有する酵母を利用した遺伝子のスクリーニング法
PL1858916T4 (pl) 2005-03-15 2015-04-30 Fujirebio Europe N V Warianty wirusa zapalenia wątroby typu b z obniżoną wrażliwością na analogi nukleozydów i ich zastosowanie
BRPI0609655A2 (pt) 2005-03-31 2010-03-16 Agensys Inc anticorpos e moléculas relacionadas que ligam-se às proteìnas 161p2f10b
CA2615581A1 (en) * 2005-07-26 2007-02-01 Merckle Gmbh Macrolide conjugates of pyrrolizine and indolizine compounds as inhibitors of 5-lipooxygenase and cyclooxygenase
JP5346583B2 (ja) * 2005-09-28 2013-11-20 アッタゲネ インコーポレーテッド 生物検体の生物活性を解析するための方法及び構築物、並びに生物の状態を測定するための方法及び構築物
WO2007042771A1 (en) * 2005-10-10 2007-04-19 Astrazeneca Ab Three hybrid screening assay using reverse transfected mammalian cell arrays
EP3124497B1 (en) 2007-09-14 2020-04-15 Adimab, LLC Rationally designed, synthetic antibody libraries and uses therefor
US12529164B2 (en) 2007-09-14 2026-01-20 Adimab, Llc Rationally designed, synthetic antibody libraries and uses therefor
US8877688B2 (en) 2007-09-14 2014-11-04 Adimab, Llc Rationally designed, synthetic antibody libraries and uses therefor
US20090162858A1 (en) * 2007-09-18 2009-06-25 Cornish Virginia W Orthogonal chemical inducer of dimerization
CN101131363B (zh) * 2007-09-30 2010-12-01 南京大学 一种基于酵母三杂交的小分子化合物检测方法
KR102024922B1 (ko) 2010-07-16 2019-09-25 아디맵 엘엘씨 항체 라이브러리

Family Cites Families (16)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1991004321A1 (en) * 1989-09-25 1991-04-04 President And Fellows Of Harvard College Receptor for fk-506
US5283173A (en) * 1990-01-24 1994-02-01 The Research Foundation Of State University Of New York System to detect protein-protein interactions
US5115098A (en) * 1990-02-28 1992-05-19 President And Fellows Of Harvard College End-blocked peptides inhibiting binding capacity of gp120
US5362629A (en) * 1991-08-05 1994-11-08 President And Fellows Of Harvard College Detection of immunosuppressants
US5498597A (en) * 1992-01-17 1996-03-12 Dana-Farber Cancer Institute, Inc. FKBP-13, an FK506-binding immunophilin
EP1978095A1 (en) * 1993-02-12 2008-10-08 The Board Of Trustees Of The Leland Stanford Junior University Chimeric receptor proteins for controlling signal transduction and ligands binding thereto
ATE207118T1 (de) * 1993-03-31 2001-11-15 Cadus Pharmaceutical Corp Hefe zellen so konstruiert, dass sie proteinsurrogate des pheromensystems produzieren und anwendungen dafuer
JPH09503645A (ja) * 1993-07-16 1997-04-15 ザ・ボード・オブ・トラスティーズ・オブ・ザ・リランド・スタンフォード・ジュニアー・ユニバーシティ 制御されたアポトーシス
EP0750630A4 (en) * 1994-03-11 1998-01-07 Ariad Pharma Inc METHOD AND MATERIALS FOR IDENTIFYING INHIBITORS OF MOLECULAR INTERACTIONS, WHICH ARE BROUGHT BY THE SH3 DOMAINS
WO1995030012A1 (en) * 1994-04-26 1995-11-09 Cadus Pharmaceutical Corporation Functional expression of mammalian adenylyl cyclase in yeast
US5989808A (en) * 1994-06-14 1999-11-23 American Cyanamid Company Identification of compounds affecting specific interaction of peptide binding pairs
EP0773952B1 (en) * 1994-07-20 2003-11-12 The General Hospital Corporation Interaction trap systems for detecting protein interactions
EP0776335A4 (en) * 1994-08-18 1999-09-15 Ariad Gene Therapeutics Inc REGULATABLE ELIMINATION OF GENE EXPRESSION, GENE PRODUCT FUNCTION AND GENE TECHNOLOGICALLY MANUFACTURED CELLS
AU3367995A (en) * 1994-08-18 1996-03-14 Ariad Pharmaceuticals, Inc. New multimerizing agents
DE69534551T2 (de) * 1994-11-01 2006-06-22 Ariad Gene Therapeutics, Inc., Cambridge Neue Methode zum Auffinden und Bewerten biologisch aktiver Moleküle
US5610015A (en) * 1995-03-23 1997-03-11 Wisconsin Alumni Research Foundation System to detect protein-RNA interactions

Also Published As

Publication number Publication date
CA2252886C (en) 2008-02-12
WO1997041255A1 (en) 1997-11-06
DE69715621T2 (de) 2003-05-22
EP0907750A1 (en) 1999-04-14
CA2252886A1 (en) 1997-11-06
DE69715621D1 (de) 2002-10-24
ATE224454T1 (de) 2002-10-15
JP2000508923A (ja) 2000-07-18
US5928868A (en) 1999-07-27
EP0907750B1 (en) 2002-09-18

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