ES2183451T3 - Complejo enzima-anticuerpo y un metodo para su fabricacion. - Google Patents

Complejo enzima-anticuerpo y un metodo para su fabricacion.

Info

Publication number
ES2183451T3
ES2183451T3 ES99105375T ES99105375T ES2183451T3 ES 2183451 T3 ES2183451 T3 ES 2183451T3 ES 99105375 T ES99105375 T ES 99105375T ES 99105375 T ES99105375 T ES 99105375T ES 2183451 T3 ES2183451 T3 ES 2183451T3
Authority
ES
Spain
Prior art keywords
group
enzyme
antibody
complex
thiol group
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
ES99105375T
Other languages
English (en)
Inventor
Hirokazu Ohabayashi
Yuriko Kitano
Takashi Kitoh
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Nichirei Corp
Original Assignee
Nichirei Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Nichirei Corp filed Critical Nichirei Corp
Application granted granted Critical
Publication of ES2183451T3 publication Critical patent/ES2183451T3/es
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/531—Production of immunochemical test materials
    • G01N33/532—Production of labelled immunochemicals
    • G01N33/535—Production of labelled immunochemicals with enzyme label or co-enzymes, co-factors, enzyme inhibitors or enzyme substrates
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2474/00—Immunochemical assays or immunoassays characterised by detection mode or means of detection
    • G01N2474/20—Immunohistochemistry assay

Landscapes

  • Health & Medical Sciences (AREA)
  • Immunology (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • Cell Biology (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

La presente invención se refiere a un complejo anticuerpos de encima donde uno o más molécula(s) de enzima, en el cual se introduce un grupo maleimido o un grupo tiol, es/son conjugados de forma covalente con un portador (polilisina, aminodextiano y demás) en el cual un grupo tiol, cuando se introduce un grupo maleimida en la enzima o cuando se introduce un grupo maleimida o un grupo tiol en la enzima mediante ellos, y un grupo maleimida se introduce al menos un grupo amino que permanece en el complejo de arriba y se conjuga de forma covalentes a un grupo maleimida del complejo de arriba mediante un grupo tiol obtenido por reducción de un fragmento anticuerpo o anticuerpo. El complejo resultante es útil como un anticuerpo marcado con una encima, particularmente en inmuno histoquímica y en test de inmunidad de enzimas y permite un ensayo de inmunidad con gran sensibilidad.
ES99105375T 1998-09-16 1999-03-16 Complejo enzima-anticuerpo y un metodo para su fabricacion. Expired - Lifetime ES2183451T3 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
JP27931998A JP3524401B2 (ja) 1998-09-16 1998-09-16 酵素抗体複合体およびその製造方法

Publications (1)

Publication Number Publication Date
ES2183451T3 true ES2183451T3 (es) 2003-03-16

Family

ID=17609526

Family Applications (1)

Application Number Title Priority Date Filing Date
ES99105375T Expired - Lifetime ES2183451T3 (es) 1998-09-16 1999-03-16 Complejo enzima-anticuerpo y un metodo para su fabricacion.

Country Status (6)

Country Link
US (1) US6252053B1 (es)
EP (1) EP0992794B1 (es)
JP (1) JP3524401B2 (es)
DE (1) DE69903899T2 (es)
DK (1) DK0992794T3 (es)
ES (1) ES2183451T3 (es)

Families Citing this family (30)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6958148B1 (en) 1998-01-20 2005-10-25 Pericor Science, Inc. Linkage of agents to body tissue using microparticles and transglutaminase
US6919076B1 (en) 1998-01-20 2005-07-19 Pericor Science, Inc. Conjugates of agents and transglutaminase substrate linking molecules
JP3781934B2 (ja) * 1999-12-22 2006-06-07 株式会社ニチレイバイオサイエンス 酵素−タンパク質複合体
US20030149246A1 (en) * 2002-02-01 2003-08-07 Russell John C. Macromolecular conjugates and processes for preparing the same
US20040248223A1 (en) * 2003-06-05 2004-12-09 Idexx Laboratories, Inc. Reduction of interfering peroxidase activity in peroxidase based detection methods
US20050186642A1 (en) 2004-02-24 2005-08-25 Biocare Medical, Inc. Immunoassay reagents and methods of use thereof
JP4920415B2 (ja) * 2004-07-30 2012-04-18 株式会社先端生命科学研究所 プローブ複合体
US20080153089A1 (en) * 2004-12-28 2008-06-26 Advanced Life Science Institute, Inc. Blocked Enzyme-Probe Complex
CA2609702C (en) 2005-04-28 2013-05-28 Ventana Medical Systems, Inc. Antibody conjugates via heterobifunctional peg linkers
CA2606018A1 (en) * 2005-04-28 2006-11-02 Ventana Medical Systems, Inc. Nanoparticle conjugates
EP1934607B1 (en) * 2005-10-13 2013-08-28 Fundação D. Anna Sommer Champalimaud E Dr. Carlos Montez Champalimaud Multiplex in situ immunohistochemical analysis
US20070117153A1 (en) * 2005-11-23 2007-05-24 Christopher Bieniarz Molecular conjugate
US20090191573A1 (en) * 2006-08-25 2009-07-30 Universite Catholique De Louvain Method and kit for determining antigen content in a sample using double immunohistochemical detection
JP5334691B2 (ja) * 2009-06-04 2013-11-06 キッコーマン株式会社 ペルオキシダーゼ化学発光測定試薬
JP5311662B2 (ja) * 2009-08-24 2013-10-09 キッコーマン株式会社 ペルオキシダーゼ化学発光反応の発光増強方法及びペルオキシダーゼ化学発光反応用発光増強剤
WO2011129357A1 (ja) 2010-04-14 2011-10-20 栄研化学株式会社 標識化プローブ－水溶性担体複合体
US8852592B2 (en) 2011-05-10 2014-10-07 Biocare Medical, Llc Systems and methods for anti-PAX8 antibodies
US10316103B1 (en) 2012-03-30 2019-06-11 Biocare Medical, Llc Systems and methods for anti-Uroplakin III antibodies
EP2900265B1 (en) 2012-09-27 2018-05-30 Biocare Medical, LLC Anti-uroplakin ii antibodies systems and methods
US10429390B2 (en) 2012-12-18 2019-10-01 Biocare Medical, Llc Antibody cocktail systems and methods for classification of histologic subtypes in lung cancer
DK2962113T3 (da) 2013-02-28 2019-07-01 Biocare Medical Llc Systemer og fremgangsmåder med anti-p40-antistoffer
JP6353446B2 (ja) 2013-06-28 2018-07-04 国立大学法人九州大学 タンパク質−ポリマ複合体、ＴＧａｓｅ基質含有ポリマ、ＴＧａｓｅ基質含有モノマ、タンパク質−ポリマ複合体の製造方法、および固液の界面あるいは界面近傍でタンパク質機能を高める方法
US9816997B2 (en) 2013-10-03 2017-11-14 Biocare Medical, Llc Anti-SOX10 antibody systems and methods
EP3136096B1 (en) 2014-04-23 2021-12-01 Nichirei Biosciences Inc. Combination for target marker detection
US11293921B2 (en) 2018-10-03 2022-04-05 Arkray, Inc. Direct electron transfer-type oxidoreductase-modified molecular recognition element
US12061200B2 (en) 2018-12-28 2024-08-13 Abbott Laboratories Direct detection of single molecules on microparticles
CN111830250A (zh) * 2020-08-04 2020-10-27 珠海市丽拓生物科技有限公司 一种信号放大的酶标二抗的制备方法
CN113391059B (zh) * 2021-06-10 2022-06-03 上海百英生物科技有限公司 一种micropolymer-HRP-纳米抗体复合物及其制备方法
CN116448996B (zh) * 2023-03-22 2023-11-21 卡秋(江苏)生物科技有限公司 一种串珠结构的多聚酶-抗体复合物的制备方法
WO2025191541A1 (en) * 2024-03-15 2025-09-18 Tocris Cookson Limited Horseradish peroxidase copolymers, conjugates, and uses thereof

Family Cites Families (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS58225028A (ja) 1982-06-25 1983-12-27 Otsuka Pharmaceut Co Ltd ヒトα型インターフェロン抗体
JPS61205863A (ja) 1985-03-09 1986-09-12 Meidensha Electric Mfg Co Ltd 酵素標識抗体の調製法およびその抗体
US5057313A (en) 1986-02-25 1991-10-15 The Center For Molecular Medicine And Immunology Diagnostic and therapeutic antibody conjugates
US5084560A (en) * 1986-06-30 1992-01-28 Oncogen Immunoconjugates and methods for their use in tumor therapy
JPH0266459A (ja) 1988-08-31 1990-03-06 Sekisui Chem Co Ltd 酵素標識抗体および酵素免疫測定法
US5053520A (en) * 1988-09-22 1991-10-01 Abbott Laboratories Heterobifunctional maleimido containing coupling agents
JPH02201162A (ja) 1989-01-30 1990-08-09 Teijin Ltd 酵素抗体複合体、複合体―抗体の組合せ、キット及び診断方法
JPH0389164A (ja) 1989-08-31 1991-04-15 Meidensha Corp 酵素免疫測定用試薬及びその調製方法
JPH0389165A (ja) 1989-08-31 1991-04-15 Meidensha Corp 酵素免疫測定用試薬及びその調製方法
JPH10507997A (ja) 1993-06-11 1998-08-04 クールター コーポレイション Ｔ細胞の活性化および増殖を誘導するための抗ｃｄ３抗体−アミノデキストラン結合体

Also Published As

Publication number Publication date
DE69903899D1 (de) 2002-12-19
EP0992794A2 (en) 2000-04-12
JP2000088850A (ja) 2000-03-31
EP0992794B1 (en) 2002-11-13
JP3524401B2 (ja) 2004-05-10
US6252053B1 (en) 2001-06-26
DE69903899T2 (de) 2003-07-17
DK0992794T3 (da) 2002-12-30
EP0992794A3 (en) 2000-04-19

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