ES2185972T3 - Sistema de expresion regulable en bacterias productoras de acido lactico. - Google Patents

Sistema de expresion regulable en bacterias productoras de acido lactico.

Info

Publication number
ES2185972T3
ES2185972T3 ES97936612T ES97936612T ES2185972T3 ES 2185972 T3 ES2185972 T3 ES 2185972T3 ES 97936612 T ES97936612 T ES 97936612T ES 97936612 T ES97936612 T ES 97936612T ES 2185972 T3 ES2185972 T3 ES 2185972T3
Authority
ES
Spain
Prior art keywords
pct
sequence
lactic acid
orientation
gene
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
ES97936612T
Other languages
English (en)
Inventor
Soeren Michael Madsen
Astrid Vrang
Jose Arnau
Peter Ravn
Johnsen Mads Groenvald
Hans Israelsen
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Danish Technological Institute
Original Assignee
Danish Technological Institute
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Danish Technological Institute filed Critical Danish Technological Institute
Application granted granted Critical
Publication of ES2185972T3 publication Critical patent/ES2185972T3/es
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • C12N15/746—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for lactic acid bacteria (Streptococcus; Lactococcus; Lactobacillus; Pediococcus; Enterococcus; Leuconostoc; Propionibacterium; Bifidobacterium; Sporolactobacillus)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/35—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Mycobacteriaceae (F)

Landscapes

  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Zoology (AREA)
  • Molecular Biology (AREA)
  • Wood Science & Technology (AREA)
  • Biophysics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Biomedical Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Microbiology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Medicinal Chemistry (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Fodder In General (AREA)

Abstract

LA PRESENTE INVENCION SE REFIERE A VECTORES DE EXPRESION QUE PUEDEN REPLICARSE EN CELULAS DE BACTERIAS LACTICAS Y QUE TIENEN UN ELEMENTO DE SECUENCIA (A) Y POR LO MENOS OTRO ELEMENTO DE SECUENCIA NUCLEOTIDO (B). LAS FUNCIONES DEL ELEMENTO DE SECUENCIA PROMOTOR (A) SE PUEDEN REGULAR POR UN FACTOR AMBIENTAL O UN FACTOR DE CONDICION DE CRECIMIENTO. LA POSICION, ORIENTACION, PRESENCIA Y/O LA SECUENCIA DEL ELEMENTO DE SECUENCIA NUCLEOTIDO (B) TIENEN UN EFECTO DE REGULACION SOBRE LA EXPRESION DE UN GEN QUE PRESENTA UNA RELACION FUNCIONAL CON LA REGION PROMOTORA EN LOS VECTORES DE LA CUAL LA POSICION, ORIENTACION, PRESENCIA Y/O LA SECUENCIA DE POR LO MENOS UNO DE LOS DOS ELEMENTOS CONSIDERADOS (A O B) SE MODIFICAN CON RELACION A LA POSICION, ORIENTACION, PRESENCIA Y/O SECUENCIA DEL ELEMENTO NO MODIFICADO CORRESPONDIENTE. POR ELLO, HAY UNA ALTERACION DE LA EXPRESION DEL GEN, Y UNA PRODUCCION DE UNA BACTERIA LACTICA QUE TIENE UN VECTOR CONFORME A LAS ESPECIFICACIONES ENUNCIADAS. LAS CELULAS DE RECOMBINACION QUE CONTIENE ESTE SISTEMA REGULABLE O ADAPTATIVO DE EXPRESION GENICA CONVIENEN EN PARTICULAR COMO CULTIVOS DE SIEMBRA DE PRODUCTOS DESTINADOS A LA ALIMENTACION HUMANA O ANIMAL, O COMO CEPAS QUE PERMITEN LA PRODUCCION DE PRODUCTOS DE INGENIERIA GENETICA, COMO POR EJEMPLO COMPUESTOS FARMACEUTICOS E INMUNOLOGICAMENTE ACTIVOS QUE TIENEN OLIGOPEPTIDOS O POLIPEPTIDOS DERIVADOS DE UNA DE LAS ESPECIES MYCOBACTERIUM, INCLUYENDO M. TUBERCULOSIS.
ES97936612T 1996-09-06 1997-08-22 Sistema de expresion regulable en bacterias productoras de acido lactico. Expired - Lifetime ES2185972T3 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US71143496A 1996-09-06 1996-09-06

Publications (1)

Publication Number Publication Date
ES2185972T3 true ES2185972T3 (es) 2003-05-01

Family

ID=24858080

Family Applications (1)

Application Number Title Priority Date Filing Date
ES97936612T Expired - Lifetime ES2185972T3 (es) 1996-09-06 1997-08-22 Sistema de expresion regulable en bacterias productoras de acido lactico.

Country Status (10)

Country Link
US (1) US6133023A (es)
EP (1) EP0925364B1 (es)
AT (1) ATE226637T1 (es)
CA (1) CA2264495C (es)
DE (1) DE69716622T2 (es)
DK (1) DK0925364T3 (es)
ES (1) ES2185972T3 (es)
NZ (1) NZ334521A (es)
PT (1) PT925364E (es)
WO (1) WO1998010079A1 (es)

Families Citing this family (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6436694B1 (en) 1998-01-09 2002-08-20 Cubist Pharmaceuticals, Inc. Regulable gene expression in gram-positive bacteria
ATE351912T1 (de) 1999-08-06 2007-02-15 Bioneer As Signalpeptide aus lactococcus lactis
AU779846B2 (en) * 1999-10-07 2005-02-17 Corixa Corporation Methods of using a mycobacterium tuberculosis coding sequence to facilitate stable and high yield expression of heterologous proteins
US7009042B1 (en) * 1999-10-07 2006-03-07 Corixa Corporation Methods of using a Mycobacterium tuberculosis coding sequence to facilitate stable and high yield expression of the heterologous proteins
DK1248536T3 (da) * 2000-01-21 2007-02-26 Hansens Lab Porphyrinholdige mælkesyrebakterieceller og anvendelse deraf
AU2002210402A1 (en) * 2000-10-20 2002-04-29 Bioteknologisk Institut Regulation of promoter activity using a polypeptide isolated from lactococcus lactis
PT1356073E (pt) * 2000-10-20 2010-02-15 Bioneer As Método de fermentação aperfeiçoado para a produção de produtos genéticos heterólogos em bactérias do ácido láctico
EP1239032A1 (en) 2001-03-02 2002-09-11 Société des Produits Nestlé S.A. Lactic acid bacteria as agents for treating and preventing allergy
KR20020090546A (ko) * 2001-05-28 2002-12-05 주식회사 알엔에이 유산균 염색체 ｄｎａ로의 삽입을 위한 삽입 벡터
EP1917278A2 (en) * 2005-08-26 2008-05-07 Cerenis Therapeutics Holding SA Compositions and methods for producing apolipoprotein gene products in lactic acid bacteria
CA2861033C (en) 2012-01-12 2017-07-25 Auxilium International Holdings, Inc. Clostridium histolyticum enzymes and methods for the use thereof
KR20240001279A (ko) 2017-03-28 2024-01-03 엔도 벤쳐즈 리미티드 개선된 콜라게나제 생성 방법
SG10201901828TA (en) * 2019-02-28 2020-09-29 Nat Univ Singapore Recombinant bacteria and uses thereof
CN119736230A (zh) * 2025-02-28 2025-04-01 山东信合生物制药有限公司 一种融合表达n-gpv vp2蛋白重组组成型乳酸菌的构建及应用

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0273040B1 (en) * 1986-03-26 1994-06-22 GX BioSystems A/S Biological containment
GB9006400D0 (en) * 1990-03-22 1990-05-23 Ciba Geigy Ag Bacterial vectors
AU652920B2 (en) * 1990-08-30 1994-09-15 Valio Ltd. Promoter probe vectors, able to replicate in E.coli, B.subtilis, lactococci and lactobacillus as well as uses thereof
WO1994016086A1 (en) * 1992-12-30 1994-07-21 Bioteknologisk Institut Recombinant lactic acid bacterium containing an inserted promoter and method of constructing same
US5837509A (en) * 1992-12-30 1998-11-17 Bioteknologisk Institut Recombinant lactic acid bacterium containing an inserted promoter and method of constructing same
JPH10500846A (ja) * 1994-05-12 1998-01-27 クウエスト・インターナシヨナル・ベー・ベー 乳酸菌（ｌａｂ）のファージから得ることができる誘導性複合プロモーターシステム及び所望のタンパク質の産生のためのｌａｂにおけるその使用
EP0712935B1 (en) * 1994-11-18 2002-06-19 Stichting Nederlands Instituut Voor Zuivelonderzoek Method for controlling the gene expression in lactic acid bacteria

Also Published As

Publication number Publication date
DK0925364T3 (da) 2003-02-24
WO1998010079A1 (en) 1998-03-12
DE69716622T2 (de) 2003-07-10
NZ334521A (en) 2000-03-27
EP0925364B1 (en) 2002-10-23
CA2264495C (en) 2007-04-17
EP0925364A1 (en) 1999-06-30
ATE226637T1 (de) 2002-11-15
DE69716622D1 (de) 2002-11-28
CA2264495A1 (en) 1998-03-12
PT925364E (pt) 2003-03-31
US6133023A (en) 2000-10-17

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