ES2187664T3 - Crioconservacion de diversas celulas vegetales. - Google Patents

Crioconservacion de diversas celulas vegetales.

Info

Publication number
ES2187664T3
ES2187664T3 ES96923280T ES96923280T ES2187664T3 ES 2187664 T3 ES2187664 T3 ES 2187664T3 ES 96923280 T ES96923280 T ES 96923280T ES 96923280 T ES96923280 T ES 96923280T ES 2187664 T3 ES2187664 T3 ES 2187664T3
Authority
ES
Spain
Prior art keywords
cells
crioconservation
vegetable
crioprotector
agent
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
ES96923280T
Other languages
English (en)
Inventor
Prakash G Kadkade
Christopher B Bare
Barbara Schnabel-Preikstas
Bin Yu
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Phyton Inc
Original Assignee
Phyton Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Phyton Inc filed Critical Phyton Inc
Application granted granted Critical
Publication of ES2187664T3 publication Critical patent/ES2187664T3/es
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N3/00—Preservation of plants or parts thereof, e.g. inhibiting evaporation, improvement of the appearance of leaves or protection against physical influences such as UV radiation using chemical compositions; Grafting wax

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • General Health & Medical Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Plant Pathology (AREA)
  • Toxicology (AREA)
  • Agronomy & Crop Science (AREA)
  • Dentistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Environmental Sciences (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Cultivation Of Plants (AREA)

Abstract

LA PRESENTE INVENCION TRATA DE PROCEDIMIENTOS PARA LA CRIOCONSERVACION DE CELULAS VEGETALES Y DE PROCEDIMIENTOS PARA RECUPERAR CELULAS VEGETALES VIABLES PARA UNA CRIOCONSERVACION A CORTO O LARGO PLAZO. LAS CELULAS VEGETALES QUE SE VAN A CRIOCONSERVAR PUEDEN SER CULTIVADAS EN UN CULTIVO Y PRETRATADAS CON UNA SOLUCION QUE CONTIENE UN AGENTE CRIOPROTECTOR, Y OPCIONALMENTE UN ESTABILIZANTE. LOS ESTABILIZANTES SON PREFERIBLEMENTE ESTABILIZANTES DE MEMBRANA, TALES COMO LOS INHIBIDORES DEL ETILENO, LOS CAPTADORES DE RADICALES DE OXIGENO, Y CATIONES DIVALENTES. LAS CELULAS TAMBIEN PUEDEN ESTABILIZARSE SOMETIENDO AL CULTIVO A UN CHOQUE DE CALOR. LAS CELULAS PRETRATADAS SE ACLIMATAN A UNA TEMPERATURA REDUCIDA Y SE CARGAN CON UN AGENTE CRIOPROTECTOR, TAL COMO DMSO, PROPILENGLICOL O POLIETILENGLICOL. LAS CELULAS CARGADAS SE INCUBAN CON UNA SOLUCION DE VITRIFICACION QUE INCLUYE, POR EJEMPLO, UNA SOLUCION CON UNA ELEVADA CONCENTRACION DEL AGENTE CRIOPROTECTOR. LAS CELULAS VITRIFICADAS RETIENEN MENOS DE UN 20 % DE CONTENIDO EN AGUA Y PUEDEN CONGELARSE A TEMPERATURAS DE CRIOCONSERVACION DURANTE LARGOS PERIODOS DE TIEMPO SIN QUE SE ALTEREN DE FORMA SIGNIFICATIVA EL CARACTER GENOTIPICO O FENOTIPICO DE LAS CELULAS. LAS CELULAS VEGETALES TAMBIEN PUEDEN CRIOCONSERVARSE MEDIANTE LA LIOFILIZACION DE LAS CELULAS ANTES DE SU EXPOSICION A LA SOLUCION DE VITRIFICACION. LA COMBINACION DE LIOFILIZACION Y VITRIFICACION ELIMINA APROXIMADAMENTE DESDE UN 80 % A UN 95 % DEL AGUA DE LAS CELULAS VEGETALES. LAS CELULAS PUEDEN SER CRIOCONSERVADAS CON EXITO DURANTE LARGOS PERIODOS DE TIEMPO Y RECUPERADAS DE FORMA VIABLE. LA INVENCION TAMBIEN TRATA DE PROCEDIMIENTOS PARA LA RECUPERACION DE CELULAS VEGETALES VIABLES PROCEDENTES DE LA CRIOCONSERVACION. LAS CELULAS SE DESCONGELAN HASTA APROXIMADAMENTE LA TEMPERATURA AMBIENTE Y SE INCUBAN EN UN MEDIO QUE CONTIENE UN AGENTE CRIOPROTECTOR Y UN ESTABILIZANTE. EL AGENTE CRIOPROTECTOR SE RETIRA Y LAS CELULAS SE INCUBAN Y SE RECUPERAN CON EXITO EN UN MEDIO DE CRECIMIENTO LIQUIDO O SEMI SOLIDO. LA INVENCION TAMBIEN TRATA DE LAS CELULAS CRIOCONSERVADAS Y DE CELULAS VEGETALES VIABLES QUE HAN SIDO RECUPERADAS PROCEDENTES DE UNA CRIOCONSERVACION A CORTO O LARGO PLAZO.
ES96923280T 1995-06-07 1996-06-07 Crioconservacion de diversas celulas vegetales. Expired - Lifetime ES2187664T3 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US08/486,204 US5965438A (en) 1995-06-07 1995-06-07 Cryopreservation of plant cells

Publications (1)

Publication Number Publication Date
ES2187664T3 true ES2187664T3 (es) 2003-06-16

Family

ID=23931007

Family Applications (1)

Application Number Title Priority Date Filing Date
ES96923280T Expired - Lifetime ES2187664T3 (es) 1995-06-07 1996-06-07 Crioconservacion de diversas celulas vegetales.

Country Status (14)

Country Link
US (4) US5965438A (es)
EP (1) EP0830059B1 (es)
JP (2) JPH11507380A (es)
KR (1) KR100763858B1 (es)
AT (1) ATE227071T1 (es)
AU (1) AU701381B2 (es)
CA (1) CA2223959A1 (es)
DE (1) DE69624701T2 (es)
DK (1) DK0830059T3 (es)
ES (1) ES2187664T3 (es)
GB (1) GB2316854B (es)
NZ (1) NZ312329A (es)
PT (1) PT830059E (es)
WO (1) WO1996039812A2 (es)

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AU6383696A (en) 1996-12-30
US5965438A (en) 1999-10-12
EP0830059A2 (en) 1998-03-25
PT830059E (pt) 2003-03-31
CA2223959A1 (en) 1996-12-19
WO1996039812A2 (en) 1996-12-19
GB9725211D0 (en) 1998-01-28
KR19990022412A (ko) 1999-03-25
JPH11507380A (ja) 1999-06-29
GB2316854B (en) 1998-11-11
DK0830059T3 (da) 2003-03-03
JP2008005846A (ja) 2008-01-17
AU701381B2 (en) 1999-01-28
ATE227071T1 (de) 2002-11-15
KR100763858B1 (ko) 2008-11-07
DE69624701T2 (de) 2003-10-16
GB2316854A (en) 1998-03-11
WO1996039812A3 (en) 1997-05-29
US6753182B1 (en) 2004-06-22
US20030031998A1 (en) 2003-02-13
US6127181A (en) 2000-10-03
DE69624701D1 (de) 2002-12-12
EP0830059B1 (en) 2002-11-06

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