ES2188926T3 - Aislamiento del acido nucleico. - Google Patents

Aislamiento del acido nucleico.

Info

Publication number
ES2188926T3
ES2188926T3 ES97915528T ES97915528T ES2188926T3 ES 2188926 T3 ES2188926 T3 ES 2188926T3 ES 97915528 T ES97915528 T ES 97915528T ES 97915528 T ES97915528 T ES 97915528T ES 2188926 T3 ES2188926 T3 ES 2188926T3
Authority
ES
Spain
Prior art keywords
nucleic acid
adsorption
phase
ionic strength
polymer
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
ES97915528T
Other languages
English (en)
Inventor
Philippe Cros
Abdelhamid Elaissari
Claude Mabilat
Christian Pichot
Marc Rodrigue
Lise Santoro
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Biomerieux SA
Original Assignee
Biomerieux SA
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Biomerieux SA filed Critical Biomerieux SA
Application granted granted Critical
Publication of ES2188926T3 publication Critical patent/ES2188926T3/es
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
    • C12N15/101—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers by chromatography, e.g. electrophoresis, ion-exchange, reverse phase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Biomedical Technology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Analytical Chemistry (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Plant Pathology (AREA)
  • Immunology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
  • Saccharide Compounds (AREA)

Abstract

METODO DE AISLAMIENTO EN FASE ACUOSA DE UN MATERIAL NUCLEICO PRESENTE EN UNA MUESTRA QUE COMPRENDE UNA ETAPA DE ADSORCION DEL MENCIONADO MATERIAL NUCLEICO SOBRE UN SOPORTE DE PARTICULAS, CARACTERIZADO PORQUE: EN UNA ETAPA (A) LLAMADA DE OBTENCION DEL REACTIVO DE ADSORCION SE DISPONE DE UN REACTIVO DE ADSORCION QUE COMPRENDE UN SOL FORMADO POR UNA FASE CONTINUA ACUOSA Y UNA FASE DISCONTINUA DE SOPORTE DE LAS PARTICULAS QUE CONSTA DE UN POLIMERO PARTICULADO, FUNCIONALIZADO OBTENIDO MEDIANTE POLIMERIZACION DE (1) UN PRIMER MONOMERO HIDROSOLUBLE DE ACRILAMIDA O UN DERIVADO DE LA ACRILAMIDA, (2) AL MENOS UN AGENTE DE RETICULACION Y (3) AL MENOS UN SEGUNDO MONOMERO, FUNCIONAL, CATIONICO E HIDROSOLUBLE; DICHO POLIMERO PRESENTA UNA TEMPERATURA CRITICA INFERIOR DE SOLUBILIDAD (LCST) PREDETERMINADA QUE ESTA COMPRENDIDA ENTRE 25 Y 45 PUESTA EN CONTACTO SE PONE EN CONTACTO EL REACTIVO DE ADSORCION CON LA MUESTRA QUE CONTIENE EL MATERIAL NUCLEICO; EN UNA ETAPA (C) LLAMADA DE ADSORCION, PARA LA PUESTA ENCONTACTO SEGUN (B), SE ELIGE AL MENOS UNO DE LOS PARAMETROS SIGUIENTES PARA EL MEDIO DE REACCION: PH AL MENOS IGUAL A 7; FUERZA IONICA AL MENOS IGUAL A 10 SUP,-2}M; TEMPERATURA INFERIOR A LA LCST DEL POLIMERO; EN UNA ETAPA (D) LLAMADA DE SEPARACION, DESPUES DE HABER OBSERVADO OPCIONALMENTE QUE LA ADSORCION SE HA PRODUCIDO, SE SEPARA LA FASE DISCONTINUA DE LA FASE CONTINUA; Y EN UNA ETAPA (E) LLAMADA DE DESORCION, SE DISOCIA POR DESORCION EL MATERIAL NUCLEICO DEL SOPORTE DE PARTICULAS AUMENTANDO LA FUERZA IONICA HASTA UN VALOR SUPERIOR A 10 SUP,-2}M.
ES97915528T 1996-03-20 1997-03-20 Aislamiento del acido nucleico. Expired - Lifetime ES2188926T3 (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
FR9603753 1996-03-20
FR9604691 1996-04-09

Publications (1)

Publication Number Publication Date
ES2188926T3 true ES2188926T3 (es) 2003-07-01

Family

ID=26232612

Family Applications (1)

Application Number Title Priority Date Filing Date
ES97915528T Expired - Lifetime ES2188926T3 (es) 1996-03-20 1997-03-20 Aislamiento del acido nucleico.

Country Status (8)

Country Link
US (1) US6737235B1 (es)
EP (1) EP0842184B1 (es)
JP (1) JP4693944B2 (es)
AT (1) ATE231875T1 (es)
CA (1) CA2222192A1 (es)
DE (1) DE69718752T2 (es)
ES (1) ES2188926T3 (es)
WO (1) WO1997034909A1 (es)

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Also Published As

Publication number Publication date
JP4693944B2 (ja) 2011-06-01
ATE231875T1 (de) 2003-02-15
JPH11505426A (ja) 1999-05-21
DE69718752T2 (de) 2003-12-24
CA2222192A1 (fr) 1997-09-25
WO1997034909A1 (fr) 1997-09-25
DE69718752D1 (de) 2003-03-06
EP0842184A1 (fr) 1998-05-20
EP0842184B1 (fr) 2003-01-29
US6737235B1 (en) 2004-05-18

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